EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 30, Issue 11, Page 1695-1706, Year 2023
掲載日	2023年9月28日
著者	Si Hoon Park / Juhyun Han / Byung-Cheon Jeong / Ju Han Song / Se Hwan Jang / Hyeongseop Jeong / Bong Heon Kim / Young-Gyu Ko / Zee-Yong Park / Kyung Eun Lee / Jaekyung Hyun / Hyun Kyu Song /
PubMed 要旨	Defects in plasma membrane repair can lead to muscle and heart diseases in humans. Tripartite motif-containing protein (TRIM)72 (mitsugumin 53; MG53) has been determined to rapidly nucleate vesicles ...Defects in plasma membrane repair can lead to muscle and heart diseases in humans. Tripartite motif-containing protein (TRIM)72 (mitsugumin 53; MG53) has been determined to rapidly nucleate vesicles at the site of membrane damage, but the underlying molecular mechanisms remain poorly understood. Here we present the structure of Mus musculus TRIM72, a complete model of a TRIM E3 ubiquitin ligase. We demonstrated that the interaction between TRIM72 and phosphatidylserine-enriched membranes is necessary for its oligomeric assembly and ubiquitination activity. Using cryogenic electron tomography and subtomogram averaging, we elucidated a higher-order model of TRIM72 assembly on the phospholipid bilayer. Combining structural and biochemical techniques, we developed a working molecular model of TRIM72, providing insights into the regulation of RING-type E3 ligases through the cooperation of multiple domains in higher-order assemblies. Our findings establish a fundamental basis for the study of TRIM E3 ligases and have therapeutic implications for diseases associated with membrane repair.
リンク	Nat Struct Mol Biol / PubMed:37770719 / PubMed Central
手法	EM (トモグラフィー) / EM (サブトモグラム平均) / X線回折
解像度	2.75 - 26.0 Å
構造データ	EMDB-31139: Reconstituted proteoliposomes of TRIM72 in negative curvature #1 手法: EM (トモグラフィー) EMDB-31150: Reconstituted proteoliposomes of TRIM72 in negative curvature #2 手法: EM (トモグラフィー) EMDB-31151: Reconstituted proteoliposomes of TRIM72 in positive curvature #1 手法: EM (トモグラフィー) EMDB-31152: Reconstituted proteoliposomes of TRIM72 in positive curvature #2 手法: EM (トモグラフィー) EMDB-33569: Higher-ordered assembly of mouse TRIM72 WT on the Phosphatidylserine/Cholesterol liposome bilayer 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-33582: Higher-ordered assembly of mouse TRIM72 M138R on the Phosphatidylserine/Cholesterol liposome bilayer 手法: EM (サブトモグラム平均) / 解像度: 26.0 Å PDB-7xv2: TRIM E3 ubiquitin ligase 手法: X-RAY DIFFRACTION / 解像度: 2.75 Å PDB-7xyy: TRIM E3 ubiquitin ligase WT 手法: X-RAY DIFFRACTION / 解像度: 7.1 Å PDB-7xyz: TRIM E3 ubiquitin ligase 手法: X-RAY DIFFRACTION / 解像度: 4.62 Å PDB-7xz0: TRIM E3 ubiquitin ligase 手法: X-RAY DIFFRACTION / 解像度: 3.28 Å PDB-7xz1: TRIM E3 ubiquitin ligase 手法: X-RAY DIFFRACTION / 解像度: 5.2 Å PDB-7xz2: TRIM E3 ubiquitin ligase 手法: X-RAY DIFFRACTION / 解像度: 3.5 Å
化合物	ChemComp-ZN: Unknown entry
由来	mus musculus (ハツカネズミ)
キーワード	MEMBRANE PROTEIN / TRIM / Tripartite motif / Ubiquitin ligase / Coiled coil / B-box / PRY-SPRY / LIGASE / METAL BINDING PROTEIN / TRIM72 / MG53