EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of an active type III-A CRISPR effector complex.
ジャーナル・号・ページ	Structure, Vol. 30, Issue 8, Page 1109-11128.e6, Year 2022
掲載日	2022年8月4日
著者	Eric M Smith / Sé Ferrell / Valerie L Tokars / Alfonso Mondragón /
PubMed 要旨	Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) provide many prokaryotes with an adaptive immune system against invading genetic material. ...Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) provide many prokaryotes with an adaptive immune system against invading genetic material. Type III CRISPR systems are unique in that they can degrade both RNA and DNA. In response to invading nucleic acids, they produce cyclic oligoadenylates that act as secondary messengers, activating cellular nucleases that aid in the immune response. Here, we present seven single-particle cryo-EM structures of the type III-A Staphylococcus epidermidis CRISPR effector complex. The structures reveal the intact S. epidermidis effector complex in an apo, ATP-bound, cognate target RNA-bound, and non-cognate target RNA-bound states and illustrate how the effector complex binds and presents crRNA. The complexes bound to target RNA capture the type III-A effector complex in a post-RNA cleavage state. The ATP-bound structures give details about how ATP binds to Cas10 to facilitate cyclic oligoadenylate production.
リンク	Structure / PubMed:35714601 / PubMed Central
手法	EM (単粒子)
解像度	3.49 - 4.97 Å
構造データ	26920 7uzw EMDB-26920, PDB-7uzw: Staphylococcus epidermidis RP62a CRISPR effector subcomplex 手法: EM (単粒子) / 解像度: 3.55 Å 26921 7uzx EMDB-26921, PDB-7uzx: Staphylococcus epidermidis RP62a CRISPR effector subcomplex with non-self target RNA bound 手法: EM (単粒子) / 解像度: 3.49 Å 26922 7uzy EMDB-26922, PDB-7uzy: Staphylococcus epidermidis RP62A CRISPR effector complex with non-self target RNA 2 手法: EM (単粒子) / 解像度: 4.05 Å 26923 7uzz EMDB-26923, PDB-7uzz: Staphylococcus epidermidis RP62a CRISPR tall effector complex 手法: EM (単粒子) / 解像度: 4.45 Å 26924 7v00 EMDB-26924, PDB-7v00: Staphylococcus epidermidis RP62a CRISPR tall effector complex with bound ATP 手法: EM (単粒子) / 解像度: 3.87 Å 26925 7v01 EMDB-26925, PDB-7v01: Staphylococcus epidermidis RP62a CRISPR short effector complex with self RNA target and ATP 手法: EM (単粒子) / 解像度: 3.67 Å EMDB-26926: Staphylococcus epidermidis RP62A CRISPR effector complex with non-self target RNA 1 手法: EM (単粒子) / 解像度: 4.06 Å 26927 7v02 EMDB-26927, PDB-7v02: Staphylococcus epidermidis RP62A CRISPR short effector complex 手法: EM (単粒子) / 解像度: 4.97 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM
由来	staphylococcus epidermidis rp62a (表皮ブドウ球菌) staphylococcus epidermidis (表皮ブドウ球菌)
キーワード	RNA BINDING PROTEIN/RNA / Type IIIA CRISPR / effector complex / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex / HYDROLASE/RNA / HYDROLASE-RNA complex