EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	FCHO controls AP2's initiating role in endocytosis through a PtdIns(4,5)P-dependent switch.
ジャーナル・号・ページ	Sci Adv, Vol. 8, Issue 17, Page eabn2018, Year 2022
掲載日	2022年4月29日
著者	Nathan R Zaccai / Zuzana Kadlecova / Veronica Kane Dickson / Kseniya Korobchevskaya / Jan Kamenicky / Oleksiy Kovtun / Perunthottathu K Umasankar / Antoni G Wrobel / Jonathan G G Kaufman / Sally R Gray / Kun Qu / Philip R Evans / Marco Fritzsche / Filip Sroubek / Stefan Höning / John A G Briggs / Bernard T Kelly / David J Owen / Linton M Traub /
PubMed 要旨	Clathrin-mediated endocytosis (CME) is the main mechanism by which mammalian cells control their cell surface proteome. Proper operation of the pivotal CME cargo adaptor AP2 requires membrane- ...Clathrin-mediated endocytosis (CME) is the main mechanism by which mammalian cells control their cell surface proteome. Proper operation of the pivotal CME cargo adaptor AP2 requires membrane-localized Fer/Cip4 homology domain-only proteins (FCHO). Here, live-cell enhanced total internal reflection fluorescence-structured illumination microscopy shows that FCHO marks sites of clathrin-coated pit (CCP) initiation, which mature into uniform-sized CCPs comprising a central patch of AP2 and clathrin corralled by an FCHO/Epidermal growth factor potential receptor substrate number 15 (Eps15) ring. We dissect the network of interactions between the FCHO interdomain linker and AP2, which concentrates, orients, tethers, and partially destabilizes closed AP2 at the plasma membrane. AP2's subsequent membrane deposition drives its opening, which triggers FCHO displacement through steric competition with phosphatidylinositol 4,5-bisphosphate, clathrin, cargo, and CME accessory factors. FCHO can now relocate toward a CCP's outer edge to engage and activate further AP2s to drive CCP growth/maturation.
リンク	Sci Adv / PubMed:35486718 / PubMed Central
手法	EM (単粒子) / EM (トモグラフィー) / EM (サブトモグラム平均) / X線回折
解像度	1.41 - 12.0 Å
構造データ	14517 7z5c EMDB-14517: Chimaera of AP2 with FCHO2 linker domain as a fusion on Cmu2 subunit PDB-7z5c: Chimera of AP2 with FCHO2 linker domain as a fusion on Cmu2 subunit 手法: EM (単粒子) / 解像度: 4.16 Å EMDB-14518: Chimaera of AP2 with FCHO2 linker domain, N1-N2 enriched population 手法: EM (単粒子) / 解像度: 6.7 Å EMDB-14525: AP2 adaptor protein recruited on the membrane in the presence of FCHO2 linker 手法: EM (トモグラフィー) / 解像度: 9.9 Å EMDB-14526: AP2 on the membrane without cargo peptide 手法: EM (サブトモグラム平均) / 解像度: 12.0 Å PDB-7ofp: Apo Structure of Mu2 Adaptin Subunit (Ap50) Of AP2 Clathrin Adaptor 手法: X-RAY DIFFRACTION / 解像度: 1.92 Å PDB-7og1: AP2 clathrin adaptor core in complex with cargo peptide and FCHO2 手法: X-RAY DIFFRACTION / 解像度: 3.25 Å PDB-7ohi: FCHO1-peptide-AP2 alpha ear complex 手法: X-RAY DIFFRACTION / 解像度: 1.41 Å PDB-7oho: Crystal structure of AP2 FCHO2 chimera 手法: X-RAY DIFFRACTION / 解像度: 2.88 Å PDB-7ohz: Crystal structure of AP2 Mu2 - FCHO2 chimera (His6-tagged) 手法: X-RAY DIFFRACTION / 解像度: 2.27 Å PDB-7oi5: Crystal structure of AP2 Mu2 - FCHO2 chimera (GST cleaved) 手法: X-RAY DIFFRACTION / 解像度: 2.61 Å PDB-7oiq: Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (C2 crystal form) 手法: X-RAY DIFFRACTION / 解像度: 1.85 Å PDB-7oit: Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (P3221 crystal form) 手法: X-RAY DIFFRACTION / 解像度: 1.65 Å
化合物	ChemComp-GOL: GLYCEROL / グリセロ-ル ChemComp-CIT: CITRIC ACID / クエン酸 ChemComp-HOH: WATER ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-MES: 2-(N-MORPHOLINO)-ETHANESULFONIC ACID / 2-モルホリノエタンスルホン酸 / pH緩衝剤YM ChemComp-IHP*: INOSITOL HEXAKISPHOSPHATE / フィチン酸
由来	Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌) rattus norvegicus (ドブネズミ) homo sapiens (ヒト) mus musculus (ハツカネズミ)
キーワード	ENDOCYTOSIS / clathrin-mediated endocytosis (CME) / YxxPhi motif / Pi(4 / 5)P2 / protein recycling / plasma membrane / PROTEIN TRANSPORT / adaptor complex / AP2 / trafficking / clathrin / CCP