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-Structure paper
タイトル | 90 pre-ribosome transformation into the primordial 40 subunit. |
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ジャーナル・号・ページ | Science, Vol. 369, Issue 6510, Page 1470-1476, Year 2020 |
掲載日 | 2020年9月18日 |
著者 | Jingdong Cheng / Benjamin Lau / Giuseppe La Venuta / Michael Ameismeier / Otto Berninghausen / Ed Hurt / Roland Beckmann / |
PubMed 要旨 | Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this ...Production of small ribosomal subunits initially requires the formation of a 90 precursor followed by an enigmatic process of restructuring into the primordial pre-40 subunit. We elucidate this process by biochemical and cryo-electron microscopy analysis of intermediates along this pathway in yeast. First, the remodeling RNA helicase Dhr1 engages the 90 pre-ribosome, followed by Utp24 endonuclease-driven RNA cleavage at site A, thereby separating the 5'-external transcribed spacer (ETS) from 18 ribosomal RNA. Next, the 5'-ETS and 90 assembly factors become dislodged, but this occurs sequentially, not en bloc. Eventually, the primordial pre-40 emerges, still retaining some 90 factors including Dhr1, now ready to unwind the final small nucleolar U3-18 RNA hybrid. Our data shed light on the elusive 90 to pre-40 transition and clarify the principles of assembly and remodeling of large ribonucleoproteins. |
リンク | Science / PubMed:32943521 |
手法 | EM (単粒子) |
解像度 | 3.5 - 7.1 Å |
構造データ | EMDB-11357, PDB-6zqa: EMDB-11358, PDB-6zqb: EMDB-11359, PDB-6zqc: EMDB-11360, PDB-6zqd: EMDB-11361, PDB-6zqe: EMDB-11362, PDB-6zqf: EMDB-11363, PDB-6zqg: |
化合物 | ChemComp-ZN: ChemComp-MG: ChemComp-GTP: |
由来 |
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キーワード | RIBOSOME / 90S pre-ribosome / 40S pre-ribosome / A1 cleavage / Dhr1 |