EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of V-ATPase from the mammalian brain.
ジャーナル・号・ページ	Science, Vol. 367, Issue 6483, Page 1240-1246, Year 2020
掲載日	2020年3月13日
著者	Yazan M Abbas / Di Wu / Stephanie A Bueler / Carol V Robinson / John L Rubinstein /
PubMed 要旨	In neurons, the loading of neurotransmitters into synaptic vesicles uses energy from proton-pumping vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases). These membrane protein complexes ...In neurons, the loading of neurotransmitters into synaptic vesicles uses energy from proton-pumping vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases). These membrane protein complexes possess numerous subunit isoforms, which complicates their analysis. We isolated homogeneous rat brain V-ATPase through its interaction with SidK, a effector protein. Cryo-electron microscopy allowed the construction of an atomic model, defining the enzyme's ATP:proton ratio as 3:10 and revealing a homolog of yeast subunit f in the membrane region, which we tentatively identify as RNAseK. The c ring encloses the transmembrane anchors for cleaved ATP6AP1/Ac45 and ATP6AP2/PRR, the latter of which is the (pro)renin receptor that, in other contexts, is involved in both Wnt signaling and the renin-angiotensin system that regulates blood pressure. This structure shows how ATP6AP1/Ac45 and ATP6AP2/PRR enable assembly of the enzyme's catalytic and membrane regions.
リンク	Science / PubMed:32165585 / PubMed Central
手法	EM (単粒子)
解像度	3.6 - 5.7 Å
構造データ	21317 6vq6 EMDB-21317: Mammalian V-ATPase from rat brain with the Legionella pneumophila effector protein SidK - rotational state 1 non-uniform refinement PDB-6vq6: Mammalian V-ATPase from rat brain - composite model of rotational state 1 bound to ADP and SidK (built from focused refinement models) 手法: EM (単粒子) / 解像度: 3.9 Å 21318 6vq7 EMDB-21318: Mammalian V-ATPase from rat brain with the Legionella pneumophila effector protein SidK - rotational state 2 non-uniform refinement PDB-6vq7: Mammalian V-ATPase from rat brain - composite model of rotational state 2 bound to ADP and SidK (built from focused refinement models) 手法: EM (単粒子) / 解像度: 4.0 Å 21319 6vq8 EMDB-21319: Mammalian V-ATPase from rat brain with the Legionella pneumophila effector protein SidK - rotational state 3 non-uniform refinement PDB-6vq8: Mammalian V-ATPase from rat brain - composite model of rotational state 3 bound to ADP and SidK (built from focused refinement models) 手法: EM (単粒子) / 解像度: 3.9 Å 21345 6vq9 EMDB-21345, PDB-6vq9: Mammalian V-ATPase from rat brain soluble V1 region rotational state 1 with SidK and ADP (from focused refinement) 手法: EM (単粒子) / 解像度: 3.6 Å 21346 6vqa EMDB-21346, PDB-6vqa: Mammalian V-ATPase from rat brain soluble V1 region rotational state 2 with SidK and ADP (from focused refinement) 手法: EM (単粒子) / 解像度: 3.7 Å 21347 6vqb EMDB-21347, PDB-6vqb: Mammalian V-ATPase from rat brain soluble V1 region rotational state 2 with SidK and ADP (from focused refinement) 手法: EM (単粒子) / 解像度: 3.6 Å 21348 6vqc EMDB-21348, PDB-6vqc: Mammalian V-ATPase from rat brain membrane-embedded Vo region rotational state 1 (from focused refinement) 手法: EM (単粒子) / 解像度: 3.8 Å 21349 6vqg EMDB-21349, PDB-6vqg: Mammalian V-ATPase from rat brain membrane-embedded Vo region rotational state 2 (from focused refinement) 手法: EM (単粒子) / 解像度: 4.2 Å 21350 6vqh EMDB-21350, PDB-6vqh: Mammalian V-ATPase from rat brain membrane-embedded Vo region rotational state 3 (from focused refinement) 手法: EM (単粒子) / 解像度: 4.4 Å 21351 6vqi EMDB-21351, PDB-6vqi: Mammalian V-ATPase from rat brain collar and peripheral stalks rotational state 1 (from focused refinement) 手法: EM (単粒子) / 解像度: 4.3 Å 21352 6vqj EMDB-21352, PDB-6vqj: Mammalian V-ATPase from rat brain collar and peripheral stalks rotational state 2 (from focused refinement) 手法: EM (単粒子) / 解像度: 5.7 Å 21353 6vqk EMDB-21353, PDB-6vqk: Mammalian V-ATPase from rat brain collar and peripheral stalks rotational state 3 (from focused refinement) 手法: EM (単粒子) / 解像度: 5.7 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	rattus norvegicus (ドブネズミ) legionella pneumophila subsp. pneumophila (strain philadelphia 1 / atcc 33152 / dsm 7513) (バクテリア)
キーワード	PROTON TRANSPORT / membrane protein complex / rotary atpase