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-Structure paper
タイトル | Co-evolution of HIV Envelope and Apex-Targeting Neutralizing Antibody Lineage Provides Benchmarks for Vaccine Design. |
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ジャーナル・号・ページ | Cell Rep, Vol. 23, Issue 11, Page 3249-3261, Year 2018 |
掲載日 | 2018年6月12日 |
著者 | Kimmo Rantalainen / Zachary T Berndsen / Sasha Murrell / Liwei Cao / Oluwarotimi Omorodion / Jonathan L Torres / Mengyu Wu / Jeffrey Umotoy / Jeffrey Copps / Pascal Poignard / Elise Landais / James C Paulson / Ian A Wilson / Andrew B Ward / |
PubMed 要旨 | Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. Longitudinal analyses of both neutralizing antibody lineages and viruses at ...Broadly neutralizing antibodies (bnAbs) targeting the HIV envelope glycoprotein (Env) typically take years to develop. Longitudinal analyses of both neutralizing antibody lineages and viruses at serial time points during infection provide a basis for understanding the co-evolutionary contest between HIV and the humoral immune system. Here, we describe the structural characterization of an apex-targeting antibody lineage and autologous clade A viral Env from a donor in the Protocol C cohort. Comparison of Ab-Env complexes at early and late time points reveals that, within the antibody lineage, the CDRH3 loop rigidifies, the bnAb angle of approach steepens, and surface charges are mutated to accommodate glycan changes. Additionally, we observed differences in site-specific glycosylation between soluble and full-length Env constructs, which may be important for tuning optimal immunogenicity in soluble Env trimers. These studies therefore provide important guideposts for design of immunogens that prime and mature nAb responses to the Env V2-apex. |
リンク | Cell Rep / PubMed:29898396 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.643 - 30.0 Å |
構造データ | EMDB-7858, PDB-6dcq: EMDB-7859: EMDB-7860: EMDB-7861: EMDB-7862: EMDB-7863: EMDB-7864: EMDB-7865: EMDB-7866: PDB-6ca6: PDB-6ca7: PDB-6ca9: |
化合物 | ChemComp-PEG: ChemComp-GOL: ChemComp-HOH: ChemComp-NAG: |
由来 |
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キーワード | IMMUNE SYSTEM / Antibody / neutralizing / IgG1 / HIV-1 / bNAb / VIRAL PROTEIN / Glycoprotein / Env |