EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular architecture of the 90S small subunit pre-ribosome.
ジャーナル・号・ページ	Elife, Vol. 6, Year 2017
掲載日	2017年2月28日
著者	Qi Sun / Xing Zhu / Jia Qi / Weidong An / Pengfei Lan / Dan Tan / Rongchang Chen / Bing Wang / Sanduo Zheng / Cheng Zhang / Xining Chen / Wei Zhang / Jing Chen / Meng-Qiu Dong / Keqiong Ye /
PubMed 要旨	Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the ...Eukaryotic small ribosomal subunits are first assembled into 90S pre-ribosomes. The complete 90S is a gigantic complex with a molecular mass of approximately five megadaltons. Here, we report the nearly complete architecture of 90S determined from three cryo-electron microscopy single particle reconstructions at 4.5 to 8.7 angstrom resolution. The majority of the density maps were modeled and assigned to specific RNA and protein components. The nascent ribosome is assembled into isolated native-like substructures that are stabilized by abundant assembly factors. The 5' external transcribed spacer and U3 snoRNA nucleate a large subcomplex that scaffolds the nascent ribosome. U3 binds four sites of pre-rRNA, including a novel site on helix 27 but not the 3' side of the central pseudoknot, and crucially organizes the 90S structure. The 90S model provides significant insight into the principle of small subunit assembly and the function of assembly factors.
リンク	Elife / PubMed:28244370 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.9 - 8.7 Å
構造データ	6695 5wyj EMDB-6695, PDB-5wyj: Cryo-EM structure of the 90S small subunit pre-ribosome (Dhr1-depleted, Enp1-TAP, state 1) 手法: EM (単粒子) / 解像度: 8.7 Å 6696 5wyk EMDB-6696, PDB-5wyk: Cryo-EM structure of the 90S small subunit pre-ribosome (Mtr4-depleted, Enp1-TAP) 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-6697: Cryo-EM structure of the 90S small subunit pre-ribosome (Noc4-TAP) 手法: EM (単粒子) / 解像度: 8.7 Å PDB-5wwn: Crystal structure of Tsr1 手法: X-RAY DIFFRACTION / 解像度: 2.805 Å PDB-5wwo: Crystal structure of Enp1 手法: X-RAY DIFFRACTION / 解像度: 2.4 Å PDB-5wxl: Crystal structure of the Rrs1 and Rpf2 complex 手法: X-RAY DIFFRACTION / 解像度: 1.9 Å PDB-5wxm: Crystal structure of the Imp3 and Mpp10 complex 手法: X-RAY DIFFRACTION / 解像度: 2.304 Å PDB-5wy3: Crystal structure of Chaetomium thermophilum Utp10 middle domain 手法: X-RAY DIFFRACTION / 解像度: 3.2 Å PDB-5wyl: Crystal structure of Chaetomium thermophilum Utp10 N-terminal domain in complex with Utp17 C-terminal helices 手法: X-RAY DIFFRACTION / 解像度: 2.638 Å
化合物	ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-HOH: WATER
由来	Saccharomyces cerevisiae (パン酵母) Baker's yeast (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) saccharomyces cerevisiae s288c (パン酵母) chaetomium thermophilum var. thermophilum dsm 1495 (菌類)
キーワード	RNA BINDING PROTEIN / ribosome biogenesis protein / component of 90S preribosome / NUCLEAR PROTEIN / protein complex / components of 90S preribosome / RIBOSOMAL PROTEIN / nucleolar protein / RIBOSOME / 90S / pre-ribosome / protein-RNA complex / RIBOSOMAL PROTEIN/NUCLEAR PROTEIN / RIBOSOMAL PROTEIN-NUCLEAR PROTEIN complex