EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms.
ジャーナル・号・ページ	Science, Vol. 387, Issue 6739, Page eadr3314, Year 2025
掲載日	2025年3月14日
著者	Xian Xia / Michelle M Shimogawa / Hui Wang / Samuel Liu / Angeline Wijono / Gerasimos Langousis / Ahmad M Kassem / James A Wohlschlegel / Kent L Hill / Z Hong Zhou /
PubMed 要旨	The flagellum of drives the parasite's characteristic screw-like motion and is essential for its replication, transmission, and pathogenesis. However, the molecular details of this process remain ...The flagellum of drives the parasite's characteristic screw-like motion and is essential for its replication, transmission, and pathogenesis. However, the molecular details of this process remain unclear. Here, we present high-resolution (up to 2.8 angstrom) cryo-electron microscopy structures of flagellar doublet microtubules (DMTs). Integrated modeling identified 154 different axonemal proteins inside and outside the DMT and, together with genetic and proteomic interrogation, revealed conserved and trypanosome-specific foundations of flagellum assembly and motility. We captured axonemal dynein motors in their pre-power stroke state. Comparing atomic models between pre- and post-power strokes defined how dynein structural changes drive sliding of adjacent DMTs during flagellar beating. This study illuminates structural dynamics underlying flagellar motility and identifies pathogen-specific proteins to consider for therapeutic interventions targeting neglected diseases.
リンク	Science / PubMed:40080582 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 7.6 Å
構造データ	EMDB-47409: Structure of 48-nm repeat DMT from Trypanosoma brucei FAP106A-KD 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-47410: Cryo-EM structure of 48-nm repeat of DMT from Trypanosoma brucei FAP106B-KD 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-47411: Cryo-EM structure of 48-nm repeat of DMT from Trypanosoma brucei PBP36-KD 手法: EM (単粒子) / 解像度: 3.5 Å 47451 9e2g EMDB-47451, PDB-9e2g: Cryo-EM structure of 48 nm repeat of microtubule doublet from T. brucei flagellum 手法: EM (単粒子) / 解像度: 2.8 Å 47524 9e5c EMDB-47524, PDB-9e5c: Cryo-EM structure of 96 nm repeat of microtubule doublet from T. brucei flagellum 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47559: Focus refine map of T. brucei DMT 48 nm repeat part1-1 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47560: Focus refine map of T. brucei DMT 48 nm repeat part1-2 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47561: Focus refine map of T. brucei DMT 48 nm repeat part1-3 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47562: Focus refine map of T. brucei DMT 48 nm repeat part1-4 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47563: Focus refine map of T. brucei DMT 48 nm repeat part1-5 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-47567: Focus refine map of T. brucei DMT 48 nm repeat part1-6 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-47568: Focus refine map of T. brucei DMT 48 nm repeat part1-7 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47569: Focus refine map of T. brucei DMT 48 nm repeat part2-1 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47574: Focus refine map of T. brucei DMT 48 nm repeat part2-2 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-47575: Focus refine map of T. brucei DMT 48 nm repeat part2-3 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47576: Focus refine map of T. brucei DMT 48 nm repeat part2-4 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47579: Focus refine map of T. brucei DMT 48 nm repeat part2-5 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-47581: Focus refine map of T. brucei DMT 48 nm repeat part2-6 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47582: Focus refine map of T. brucei DMT 48 nm repeat part2-7 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-47590: Overall map of T. brucei DMT 48 nm repeat 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-47591: Focus refine map of T. brucei DMT 96 nm repeat DMT part1 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-47593: Focus refine map of T. brucei DMT 96 nm repeat DMT part2 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47594: Focus refine map of T. brucei DMT 96 nm repeat radial spoke 1 stalk 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-47595: Focus refine map of T. brucei DMT 96 nm repeat DMT part3 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47596: Focus refine map of T. brucei DMT 96 nm repeat IDA-a base 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-47597: Focus refine map of T. brucei DMT 96 nm repeat IDA-b base 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-47598: Focus refine map of T. brucei DMT 96 nm repeat radial spoke 2 stalk 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-47599: Focus refine map of T. brucei DMT 96 nm repeat N-DRC base 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47602: Focus refine map of T. brucei DMT 96 nm repeat radial spoke 3 base 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-47603: Focus refine map of T. brucei DMT 96 nm repeat radial spoke 3 MOP84 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-47621: Focus refine map of T. brucei DMT 96 nm repeat radial spoke 1 head region lobe1 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-47623: Focus refine map of T. brucei DMT 96 nm repeat radial spoke 1 head region lobe2 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-47625: Focus refine map of T. brucei DMT 96 nm repeat radial spoke 2 head region lobe1 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-47626: Focus refine map of T. brucei DMT 96 nm repeat radial spoke 2 head region lobe2 手法: EM (単粒子) / 解像度: 6.9 Å EMDB-47629: Focus refine map of T. brucei DMT 96 nm repeat ODA IC-LC region 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-47631: Focus refine map of T. brucei DMT 96 nm repeat ODA-beta motor domain 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-47632: Focus refine map of T. brucei DMT 96 nm repeat ODA-alpha motor domain 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47634: Focus refine map of T. brucei DMT 96 nm repeat IDAf IC-LC region 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-47637: Focus refine map of T. brucei DMT 96 nm repeat IDAf head region 手法: EM (単粒子) / 解像度: 7.2 Å EMDB-47652: Focus refine map of T. brucei DMT 96 nm repeat IDA-a 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-47653: Focus refine map of T. brucei DMT 96 nm repeat IDA-b 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-47654: Focus refine map of T. brucei DMT 96 nm repeat N-DRC distal region 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-47656: overall map of T. brucei DMT 96 nm repeat 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-47773: Focus refine map of T. brucei 48 nm repear FAP106A-KD part1-1 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-47778: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part1-2 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-47779: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part1-3 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-47780: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part1-4 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-47781: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part1-5 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-47782: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part1-6 手法: EM (単粒子) / 解像度: 5.4 Å EMDB-47783: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part1-7 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-47784: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part2-1 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-47785: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part2-2 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-47786: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part2-3 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-47787: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part2-4 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-47788: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part2-5 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-47789: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part2-6 手法: EM (単粒子) / 解像度: 5.4 Å EMDB-47790: Focus refine map of T. brucei 48 nm repeat FAP106A-KD part2-7 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-47794: Overall map of T. brucei 48 nm repeat FAP106A-KD 手法: EM (単粒子) / 解像度: 5.8 Å EMDB-47803: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part2-5 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-47806: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part1-1 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47807: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part1-2 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47808: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part1-3 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-47809: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part1-4 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47810: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part1-5 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-47811: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part1-6 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-47812: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part1-7 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-47813: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part2-1 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47814: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part2-2 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47815: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part2-3 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-47816: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part2-4 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-47817: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part2-6 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-47818: Focus refine map of T. brucei 48 nm repeat FAP106B-KD part2-7 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-47819: Overall map of T. brucei 48 nm repeat FAP106B-KD 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-47859: Focus refine map of T. brucei 48 nm repeat PBP36-KD part1-1 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47860: Focus refine map of T. brucei 48 nm repeat PBP36-KD part1-2 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47861: Focus refine map of T. brucei 48 nm repeat PBP36-KD part1-3 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-47862: Focus refine map of T. brucei 48 nm repeat PBP36-KD part1-4 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47863: Focus refine map of T. brucei 48 nm repeat PBP36-KD part1-5 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-47864: Focus refine map of T. brucei 48 nm repeat PBP36-KD part1-6 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-47865: Focus refine map of T. brucei 48 nm repeat PBP36-KD part1-7 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-47866: Focus refine map of T. brucei 48 nm repeat PBP36-KD part2-1 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47867: Focus refine map of T. brucei 48 nm repeat PBP36-KD part2-2 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-47868: Focus refine map of T. brucei 48 nm repeat PBP36-KD part2-3 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47869: Focus refine map of T. brucei 48 nm repeat PBP36-KD part2-4 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-47870: Focus refine map of T. brucei 48 nm repeat PBP36-KD part2-5 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-47871: Focus refine map of T. brucei 48 nm repeat PBP36-KD part2-6 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-47872: Focus refine map of T. brucei 48 nm repeat PBP36-KD part2-7 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-47873: Overall map of T. brucei 48 nm repeat PBP36-KD 手法: EM (単粒子) / 解像度: 5.0 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM
由来	trypanosoma brucei brucei treu927 (トリパノソーマ)
キーワード	MOTOR PROTEIN / flagella / microtubule