EMDB-47451: Cryo-EM structure of 48 nm repeat of microtubule doublet from T. ...

Basic information

Entry

Database: EMDB / ID: EMD-47451

• Title: Cryo-EM structure of 48 nm repeat of microtubule doublet from T. brucei flagellum

Sample

• Organelle or cellular component: Trypanosoma brucei flagellum
  • Protein or peptide: x 60 types
• Ligand: x 4 types

• Keywords: flagella / microtubule / MOTOR PROTEIN

Function / homology

Function:

intraciliary transport particle / microtubule anchoring / cilium-dependent cell motility / regulation of cilium beat frequency involved in ciliary motility / axonemal microtubule / negative regulation of microtubule depolymerization / nucleoside-diphosphate kinase / ciliary plasm / nuclear lumen / UTP biosynthetic process / CTP biosynthetic process / motile cilium / nucleoside diphosphate kinase activity / GTP biosynthetic process / positive regulation of cell motility / post-transcriptional regulation of gene expression / cyclosporin A binding / cortical cytoskeleton / phosphate ion binding / mitotic cytokinesis / axoneme / cilium assembly / alpha-tubulin binding / cytoplasmic microtubule / Hsp70 protein binding / mitotic spindle organization / meiotic cell cycle / RNA polymerase II CTD heptapeptide repeat P3 isomerase activity / RNA polymerase II CTD heptapeptide repeat P6 isomerase activity / peptidylprolyl isomerase / Hsp90 protein binding / structural constituent of cytoskeleton / microtubule cytoskeleton organization / mitotic spindle / protein folding / mitotic cell cycle / microtubule binding / microtubule / cytoskeleton / hydrolase activity / calmodulin binding / ciliary basal body / cilium / ribosome / GTPase activity / calcium ion binding / GTP binding / mitochondrion / nucleoplasm / ATP binding / metal ion binding / nucleus / cytoplasm

Domain/homology:

Cilia- and flagella-associated protein 97 / Uncharacterized 22kDa protein in aldolase locus / Tubby, N-terminal / Hemingway/CFA97 / Hemingway/CFA97 / CCDC81, HU domain 1 / Protein of unknown function DUF4586 / CCDC81, HU domain 2 / CCDC81 eukaryotic HU domain 1 / Cilia-and flagella-associated protein 96 / CCDC81 eukaryotic HU domain 2 / : / Cilia- and flagella-associated protein 161-like domain / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Enkurin domain / : / Calmodulin-binding / Enkurin domain profile. / : / CFA20 domain / Cilia- and flagella-associated protein 20/CFAP20DC / CFA20 domain / Parkin co-regulated protein / Parkin co-regulated protein / Nucleoside diphosphate kinase-like domain / Nucleoside diphosphate kinase / NDK / Nucleoside diphosphate kinase-like domain superfamily / Quinoprotein alcohol dehydrogenase-like superfamily / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain profile. / Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain / Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD / Cyclophilin-like domain superfamily / cAMP/cGMP binding motif profile. / Cyclic nucleotide-binding domain / Tubulin-beta mRNA autoregulation signal. / Alpha tubulin / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / EF-hand domain pair / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Armadillo-type fold / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / WD domain, G-beta repeat / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily

Component:

Peptidyl-prolyl cis-trans isomerase / Calpain-like cysteine peptidase, putative / Trichohyalin-plectin-homology domain-containing protein / CCDC81 HU domain-containing protein / Uncharacterized protein / Uncharacterized protein / EF-hand domain-containing protein / Uncharacterized protein / T. brucei spp.-specific protein / Cilia- and flagella-associated protein 52 / Uncharacterized protein / Flagellar associated protein / Uncharacterized protein / Enkurin domain-containing protein / Trichohyalin-plectin-homology domain-containing protein / Uncharacterized protein / DM10 domain-containing protein / Cyclic nucleotide-binding domain-containing protein / EF-hand domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / Rib72 protein-like protein / Uncharacterized protein / EF-hand domain-containing protein / KIAA1430 homologue / Uncharacterized protein / CFA20 domain-containing protein / Nucleoside diphosphate kinase, putative / Uncharacterized protein / T. brucei spp.-specific protein / EF-hand domain-containing protein / Tubulin alpha chain / Tubulin beta chain / Enkurin domain-containing protein / Uncharacterized protein / Flagellar protofilament ribbon protein, putative / Cilia- and flagella-associated protein 45 / Uncharacterized protein / Cilia- and flagella-associated protein 53 / EF-hand domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein / STOP axonemal protein / Uncharacterized protein / Starmaker / Uncharacterized protein / EF-hand domain-containing family member C2 / Uncharacterized protein / EF-hand domain-containing protein / Nucleoside diphosphate kinase, putative / Uncharacterized protein / Uncharacterized protein / Enkurin domain-containing protein / Enkurin domain-containing protein / Calcium-binding protein, putative / Uncharacterized protein / Meiosis-specific nuclear structural protein 1

• Biological species: Trypanosoma brucei brucei TREU927 (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 2.8 Å
• Authors: Xia X / Shimogawa MM / Wang H / Liu S / Wijono A / Langousis G / Kassem AM / Wohlschlegel JA / Hill K / Zhou ZH

Funding support

United States, 2 items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	R01GM071940	United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	R01AI052348	United States

• Citation: Journal: Science / Year: 2025; Title: Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms.; Authors: Xian Xia / Michelle M Shimogawa / Hui Wang / Samuel Liu / Angeline Wijono / Gerasimos Langousis / Ahmad M Kassem / James A Wohlschlegel / Kent L Hill / Z Hong Zhou / ; Abstract: The flagellum of drives the parasite's characteristic screw-like motion and is essential for its replication, transmission, and pathogenesis. However, the molecular details of this process remain unclear. Here, we present high-resolution (up to 2.8 angstrom) cryo-electron microscopy structures of flagellar doublet microtubules (DMTs). Integrated modeling identified 154 different axonemal proteins inside and outside the DMT and, together with genetic and proteomic interrogation, revealed conserved and trypanosome-specific foundations of flagellum assembly and motility. We captured axonemal dynein motors in their pre-power stroke state. Comparing atomic models between pre- and post-power strokes defined how dynein structural changes drive sliding of adjacent DMTs during flagellar beating. This study illuminates structural dynamics underlying flagellar motility and identifies pathogen-specific proteins to consider for therapeutic interventions targeting neglected diseases.

History

Deposition	Oct 22, 2024	-
Header (metadata) release	Mar 12, 2025	-
Map release	Mar 12, 2025	-
Update	Mar 26, 2025	-
Current status	Mar 26, 2025	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_47451.map.gz / Format: CCP4 / Size: 329.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.1 Å

Density

Contour Level	By AUTHOR: 0.17
Minimum - Maximum	0.0 - 1.8754638
Average (Standard dev.)	0.03525728 (±0.10453439)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 326 454 583 Spacing 454 326 583
Cell	A: 499.40002 Å / B: 358.6 Å / C: 641.3 Å α=β=γ: 90.0 °

Supplemental data

Additional map: part1-7

• File: emd_47451_additional_1.map
• Annotation: part1-7

Additional map: part2-3

• File: emd_47451_additional_10.map
• Annotation: part2-3

Additional map: part2-4

• File: emd_47451_additional_11.map
• Annotation: part2-4

Additional map: part2-5

• File: emd_47451_additional_12.map
• Annotation: part2-5

Additional map: part2-6

• File: emd_47451_additional_13.map
• Annotation: part2-6

Additional map: part2-7

• File: emd_47451_additional_14.map
• Annotation: part2-7

Additional map: part1-2

• File: emd_47451_additional_2.map
• Annotation: part1-2

Additional map: part1-5

• File: emd_47451_additional_3.map
• Annotation: part1-5

Additional map: part1-6

• File: emd_47451_additional_4.map
• Annotation: part1-6

Additional map: part1-4

• File: emd_47451_additional_5.map
• Annotation: part1-4

Additional map: part1-1

• File: emd_47451_additional_6.map
• Annotation: part1-1

Additional map: part1-3

• File: emd_47451_additional_7.map
• Annotation: part1-3

Additional map: part2-1

• File: emd_47451_additional_8.map
• Annotation: part2-1

Additional map: part2-2

• File: emd_47451_additional_9.map
• Annotation: part2-2

Sample components

Entire : Trypanosoma brucei flagellum

• Entire: Name: Trypanosoma brucei flagellum

Components

• Organelle or cellular component: Trypanosoma brucei flagellum
  • Protein or peptide: Tubulin beta chain
  • Protein or peptide: Tubulin alpha chain
  • Protein or peptide: MC7
  • Protein or peptide: FAP96C/MC15
  • Protein or peptide: MOP23C
  • Protein or peptide: KIAA1430 homologue
  • Protein or peptide: Starmaker
  • Protein or peptide: PON4
  • Protein or peptide: MOP23B
  • Protein or peptide: PON3
  • Protein or peptide: MOP23A
  • Protein or peptide: Enkurin domain-containing protein
  • Protein or peptide: Enkurin domain-containing protein
  • Protein or peptide: FAP96B
  • Protein or peptide: PBP36
  • Protein or peptide: MC4
  • Protein or peptide: CCDC81 HU domain-containing protein
  • Protein or peptide: Enkurin domain-containing protein
  • Protein or peptide: MC5
  • Protein or peptide: Enkurin domain-containing protein
  • Protein or peptide: Trichohyalin-plectin-homology domain-containing protein
  • Protein or peptide: MC8
  • Protein or peptide: STOP axonemal protein
  • Protein or peptide: Trichohyalin-plectin-homology domain-containing protein
  • Protein or peptide: MC3
  • Protein or peptide: Cilia- and flagella-associated protein 45
  • Protein or peptide: FAP90
  • Protein or peptide: Flagellar associated protein
  • Protein or peptide: EF-hand domain-containing protein
  • Protein or peptide: FAP107/MC11
  • Protein or peptide: Peptidyl-prolyl cis-trans isomerase
  • Protein or peptide: FAP129
  • Protein or peptide: Calpain-like cysteine peptidase, putative
  • Protein or peptide: FAP21
  • Protein or peptide: T. brucei spp.-specific protein
  • Protein or peptide: T. brucei spp.-specific protein
  • Protein or peptide: EF-hand domain-containing protein
  • Protein or peptide: TbRib26b
  • Protein or peptide: Cilia- and flagella-associated protein 20
  • Protein or peptide: PACRGB
  • Protein or peptide: PACRGA
  • Protein or peptide: Cilia- and flagella-associated protein 52
  • Protein or peptide: Nucleoside diphosphate kinase, putative
  • Protein or peptide: TbMIP23
  • Protein or peptide: Cyclic nucleotide-binding domain-containing protein
  • Protein or peptide: EF-hand domain-containing protein
  • Protein or peptide: EF-hand domain-containing protein
  • Protein or peptide: FAP141
  • Protein or peptide: Nucleoside diphosphate kinase, putative
  • Protein or peptide: FAP95/MC6
  • Protein or peptide: Calcium-binding protein, putative
  • Protein or peptide: EF-hand domain-containing protein
  • Protein or peptide: EF-hand domain-containing protein
  • Protein or peptide: Flagellar protofilament ribbon protein, putative
  • Protein or peptide: Cilia- and flagella-associated protein 53
  • Protein or peptide: Meiosis-specific nuclear structural protein 1
  • Protein or peptide: EF-hand domain-containing family member C2
  • Protein or peptide: EF-hand domain-containing family member C2
  • Protein or peptide: Rib72 protein-like protein
  • Protein or peptide: CMF34/CARP4
• Ligand: ZINC ION
• Ligand: GUANOSINE-5'-TRIPHOSPHATE
• Ligand: MAGNESIUM ION
• Ligand: GUANOSINE-5'-DIPHOSPHATE

Supramolecule #1: Trypanosoma brucei flagellum

• Supramolecule: Name: Trypanosoma brucei flagellum / type: organelle_or_cellular_component / ID: 1 / Parent: 0 ; Macromolecule list: #60, #59, #51-#52, #56-#58, #50, #55, #49, #54, #47-#48, #53, #46, #36, #38, #34, #37, #35, #41, #43, #42, #40, #44, #39, #45, #28, #21-#22, #20, #25, #16, #27, #26, #23, #19, #29, #32, #31, #30, #33, #8, #13, #12, #11, #10, #14, #9, #24, #18, #15, #17, #5-#7, #1-#4; Details: Demembraned flagella were split by mild protease treatment
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)

Macromolecule #1: EF-hand domain-containing family member C2

• Macromolecule: Name: EF-hand domain-containing family member C2 / type: protein_or_peptide / ID: 1 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 86.973781 KDa

Sequence

String:

MAYQQSRALK KEFSLPMVPG MTCGEEMLRR SYHRTQVHGR KYDTNTHIDG VPEDMSRFNL QTVSSISKYA PEMLPTATQT QKSDEQNVD LTGRVLRFYA YTKELVPESF VERERVRKFV FNVFLEDNTM SVVEDVADNS GIAMPASLKR HIVPLPDGSP I TFANFRVG ETITFYGRTY MVYDADKFTR DFYSQSGLEL DPALPLPFDA YTELQNRPKK IYAVRTIAAS DPTNLTLLPE QV RATQQFL KHDGEVLRCD CVWDDMEALH GTKHYLTLYY FLSDDSIALV EKDYPNSGRD PFPRFFRRQR VAKPKDGRFD PTS LGTLTF EDTSNRDYYT DADIRIGNCL HVFGRDVLIY DYDEYTQHHL LKKFGITSYD PIPGGKNPPA APIGCHRREK TAQE LEEVQ MRKRAENRMR EYGDVTVKFL MRLDNAKYED EIRRFVLTVY PADDTISIFE PVIRNMGIVG GKFLQRQRSK RPNGE FYTA KDFFVGARLT INGFPFVILS SDERSLSYME TKHDEFIRSD INYVVRKLRA MLLSRKTGLV EAFREADKEN STGLKM DVF LDIMNRLKLD ISEQELLSLL RYFDKQNESY VSYEEFMSRV MPEGVAVASD DRPWEVIDAQ SAEEELAAFV VDPRIDE EK RLRAEQISLA ARGAEEFLTL YDQRRQLVLK EFRAMTDYSP EGVIGAKEFK MCIRRKLFVQ TIPDAALDAL CDKLFPPE M PKLSLEELTR VFNGTSTLPR NMKDIKAGES K

UniProtKB: DM10 domain-containing protein

Macromolecule #2: EF-hand domain-containing family member C2

• Macromolecule: Name: EF-hand domain-containing family member C2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 88.366906 KDa

Sequence

String:

MNSTSTSSYE HDVPVKYIRT LDARLLPPRV GHNWLDAAFR SVQGKPQQLE EEFRGKRAFM PPGVYDHTPP EGLGLTARQL MQALSTRRK DGPAPDGRPI FTTLSDKVLR FYAFFSEKAP EGCCEEYWHR CVVINFYPED DTVLIQEPPI PNSGLPGGTF L KRQKVRAD PRQREQFPSD EFLTINHFNV GYSVRINCVE FFLYDCDAFT RDFLTEIGVD VGEPMQYPDS SFMSQWKHQQ EQ RATTNYG IVSNNYYRDD AVRAARFVLD AGKVLRFYGL LDERDKTTGG AVRKLEVLYF VEDDSIAVVE RPTTNEAVPA LFL SRGWLP KAGSIEKTLE FTFAHRVNGM REPYVGPGGC YTARDLGVGA TINVLGRGVF LYDCDDFTRS YYKETFGVEL AEAI DGLSQ YGLPSKPDVV SFRSNATPAS APATVAPKVV ETNVIIKKGD VLRFLLRLSA PCTSAERMRR FTLTHYTATG DSMVY ESPI KNSGYVGGCF SSRSRIPNPA GGPGAYYTHE DFKVGSIIVI NAHKFEVMNM DEHTANFLAC KGETALNEEQ LRLLVD AFR LFLRTRFHSF RDAFLGFDRD KDSVISVTEF VDHVTHLQIT DRRMDAQALF DSICQNPETG YLTLETFVDW INQKGSE SC GDAKSSQPIN IDERALMRKA LCQLCERLEA RCLNSLQMFR LASTMPRAYS GRRADCYSLT NPHRDAYITP VQLRRCIE E VLGGNPSPRE LDALLFFFFP ALPPEEYRVK RDISLEHSLD LKAFQKKYHE MCTLQQLS

UniProtKB: EF-hand domain-containing family member C2

Macromolecule #3: Rib72 protein-like protein

• Macromolecule: Name: Rib72 protein-like protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 82.953188 KDa

Sequence

String:

MTDRTIYDAL PKLPGFSFPE LNPPPTNGIP QLCTIRKGIR TVFDPSGSSK RSEKQGGDGC GGLQPAQIFA KFPDWKSLDD KALRFFGYY VERVDESSIE KMRVRKVKMY LHLSDGSISV YETPAVVNSG LRRGLTVSRT IIDGVGVRSL FVGSVVNIRG L QYHIVDCD GATREFCEAM GIPQAEPLDY PSDTFEQSVL VQRNPKDELH VDLRHNVEVM AATAAGTHVS LLTPEERETA RN FFEHDRE VLRFAATWEQ RAFKLLYYIA DKTMSVMVES VRNDGRDPNP VFIRRTKIPK YPVTRVKETE TLNVPLTRPV EYI TEDDLQ TGQTINLMTR EFYIYDCDKF TRDYYAAKGV GQPSFPKPKT ESDSLMPTVK PSKQPTKPTA RKEARGVSTM TFED TVVEK DRLKLIHYCN DVFRFAARLV SDRYEDEGRK FLFCYYLADD TVGMYEIPVH NSGHLGGKCF ARSPVAEIPE PSKLY VGAK VKLAGAEYEL IDMDERTKRY IEMGFPHMDE SYFSTQELIG HVKNVIFQRF SNVTDAFRHF KSREEGLTGE DLKRLF LEC GRRLDAAEFD RVMASVDKDN DQIISMTEFC ENLLCQQFLS DFSQTKDNGL PNVSGPLRSQ QDLEAYKNRE KEAHEAL RN LISCVEARRT LLIRAFQQEA NASYDGNLAM EDFKRALTER MGLTFTDKQM DSLIFKFYSV PGTTDWSRRR LPLKEIKR L IMF

UniProtKB: Rib72 protein-like protein

Macromolecule #4: CMF34/CARP4

• Macromolecule: Name: CMF34/CARP4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 90.251391 KDa

Sequence

String:

MKNSVARTQP VRKYENFTLE NNLPLALGAN FHDDPICRDT NRTSHTLLLP RNVDYAPHTE YVFNGGGFNR ENKMRTIDEE FVDEDEPVF DGWMTVNFDN PDDAKDHVVS FLAYFVEDIP EGTETKIVRK VCIRYYTQDN SISVQEAKQQ NSGIVQSTIL S RRQVPRRM DNINDIVMLE DFQIGGTITL FSREYHILDM DARSRLYYKK VLGQTVPEPL PWPIEIDKFT TMQAQLSKST HR LATSEDM DQKRAIEQQL TGIYTKHPTE DILTAQNFLR HNINEHLTFL ALWDDRESLS GDLRFVVIRL YLENNTVEII ERR QENSGR MGSSVILGRQ RVARPGAEGS KIRFQEHTFG VILKRDFLVA EDMKVGETYH IHGRPYFIYD ADEATRRYMK NELG IELAP CVDIKPILAS DEKKPIIFFP PPPNGFGSER ENRSSWLTLN PRPMRRDVEK IEKEEGRVMN FLAELANPLV RGDEK RRFV ISFFRETDEM SIYEKPERNS GYLAGRFLAK GVYRKPMPDG STVPYTAEDF QVGKEITILE RPFRLLDMSE ETKRIL TVT EQLPSEQRLK ELLLLFKQQI QLKFTRGHEA YCTLAPKGVL GYRQVREFLR SCSCSITEDE ALLLVHNLVP SSAGVIS FN EFMDLVNITS SEHMDEASLT VRSVKSVNMT KDESLKTVAI KTEDVKRRKQ LAVELRQKLI QRKGSVQEQF RLIGCHSA S SRLNRDVFRH SLNEVMHFNV PKTDEDMLVS LLFDGRADEN GDITYKQFQE FLEVQDDI

Macromolecule #5: Flagellar protofilament ribbon protein, putative

• Macromolecule: Name: Flagellar protofilament ribbon protein, putative / type: protein_or_peptide / ID: 5 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 46.95675 KDa

Sequence

String:

MEPAAETINV ASNSSSLPQL ETATTREKKR LFMMQRAERL KDPKMRHMGI DKEALDRQVR EREALRQLEK ERNDFYDRQA LLMDRHAQA LQKEVNEIRA NREKQLLDYR ETYQKKETQR EWDLNDPHWK AKDLPGRVGD NDPRTGVSSL QKFEGEDLDY K NRRAAQQR QQREWARQQT EEKLAKKWME EEANRVFDER NEETNRRIYD IEQGIAEQRR MIHKNQAEFN KALAEQKRRE AI RDKEEDT RKALEEIRFH MEGDFLNETE TVVSELGKKV KAERYKGMTE EQKRKFLEDR ARQRDLLRRR RFMEVEEERR WAQ QDNLQL RMANALERQK ERERHAERLS IAAEQMKQRE ASQIRKKQLD ELYTNQVDED YFKYWDLCM

UniProtKB: Flagellar protofilament ribbon protein, putative

Macromolecule #6: Cilia- and flagella-associated protein 53

• Macromolecule: Name: Cilia- and flagella-associated protein 53 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 58.689828 KDa

Sequence

String:

MAHVAIRRQR EEEQRAREQA QAVEKRMRLA ANFETRSEKV YEQKDLMRRL DLVRAKHDDA LVARRQRLAA MLLREKEEHE AMLNNLTET DEQRRDRLIR KARELRAQQQ HHLRVDAQKR HERLFREKID CLRLAESRLR VMQVANARFE QLALAERRKE E QQREEEFF AQQRVEENRL ANERAQKDLE EDYIRKQAVV KALAAQVEGN KMRAEQHQLE VKKENEAFCR AVEEERAAEA QK KMEARIA RAALAKEMSE FNEQLRTARR QEYERLQKED REVLDRMLAE LAEQEQEEKR RKHELRANAR LHLKEVERQM NQR KEDMEN LDKLWEEENN KVWEKREAHW RADEEKRRKL LRNVLIVRRQ QVLDKRQQEK EAVERAEVER QEFRNMIAGL ADID AMERA QRFAVAKENQ KYLESQVQRR NAEKEEVRMA MKTALTAEQE KEKVHAERIK REIENLERAK PERYKDVPLL PRQRF PPI

UniProtKB: Cilia- and flagella-associated protein 53

Macromolecule #7: Meiosis-specific nuclear structural protein 1

• Macromolecule: Name: Meiosis-specific nuclear structural protein 1 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 52.699703 KDa

Sequence

String:

MDAGDARVER LRRVNRYKAV QAELAREREE AEFQAMRERK ISAAARDEAL AKELAERQRL ELKDAKMLQF VRDLPELRNL EAQLKHARM KVDRSDQVDE CCKRREERLQ EEREYNAYLA EKEAKEKAEE EEKRRKAIQA FNEHQAAQLK LIEERRAQAE R DAEQSRQE RFAVDAVAAR LQEKEFLEAL ERREKQRQLQ AEQDEFYRLR KEIKENERLR QQREDEAIEA YLAEKGRRRE TD EKLLREK EAVKARILEE QSKKIMEERL KREELESLLS DYYEAERISR ERQALADAKE RSEKLADAVK QENWNLIQDR IKA RDLERQ EEAMMRQKAV EDLAQQAKAK RLERERQIEI KKQKILETER RLEKFQELKR EEQRLAAEVE ERERKRAEEL QEYI RRARA QLLEEYVPTL GQHVPARLLT VDEKKKFGIT K

UniProtKB: Meiosis-specific nuclear structural protein 1

Macromolecule #8: Nucleoside diphosphate kinase, putative

• Macromolecule: Name: Nucleoside diphosphate kinase, putative / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO / EC number: nucleoside-diphosphate kinase
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 37.147473 KDa

Sequence

String:

MTALERYAFK VDYCDPQANL VRQYLLLYFA EDSTIEMHDL KTKRVFLKRC AYPSLTPREL FIGATVGVFS RSLKLVDYGD EVTRRHFSG SEAEFVVFIQ EGGLCHMGSI IDRMHTWELR ITNIRLVDLP DSLCRDLGVS RRCVAILFKG SNAIEKVGGL S TEFPNMTV VVAEPSDVNS VRGAAFGPGG TTAVMKNCSV CVIKPHAIMS GYQGAIIQRL IDEGFHITAL GMYSLTVADA ED FLEVYNG VVPEYQRLVE QMSSGPCWAV QVCAENSVSA LRAICGPHDP DVCHVLFPHT IRSKYGVDRT RNGVHCTDLE EDA PLESEF FFSLLQNA

UniProtKB: Nucleoside diphosphate kinase, putative

Macromolecule #9: Nucleoside diphosphate kinase, putative

• Macromolecule: Name: Nucleoside diphosphate kinase, putative / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO / EC number: nucleoside-diphosphate kinase
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 38.993656 KDa

Sequence

String:

MARSLQDPRL SFYCEQYDHI AHRMNHYVLQ FYFEDRTVEI REVTKNRLHL KRAHFPHLNR DDFKVGSSLS LLGGVIKLTA YADEVTREL CGERGEVTAV MFGEQLLPQL GRCLAVLTEE CGFVALEMQM AWLPVETAAA YGVPPDLVEG RIVVVKCANT N ALQRGIDF MARMPGARAA ESVEEVGRWE QIVEKAKEQP VAILGDPNST VVIIKPHALQ KLAGGVIVQQ LIDAGLEISG IS LTNMTSQ QANELLKPYK GVLPDFPDTM RSLMGTVWVL QFVSLDEGVD VVSVAREVCG PFDPVIAKEL RPTSIRARFG VDR AHNAVH CCDLHEEGPL YSNFFFRPED VDE

UniProtKB: Nucleoside diphosphate kinase, putative

Macromolecule #10: EF-hand domain-containing protein

• Macromolecule: Name: EF-hand domain-containing protein / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 47.731914 KDa

Sequence

String:

MPLLYASRAK HTRFKSIVQR TRRLLCNGAS GANGIRKLSR GCGIAVDSGG QSMEKAKFVE ALEESGVSLD SEDIEAIVHV LDRSGDGVL DPTDFIAALR RNLTPLKLTW ITRVWYTFTQ SKDGSVYIDE VLSSYNAAGH PDVVQNIRSE QGVRSEFEAA F STTTNPDG AITRQEFEQY CSGVAALCAN DLEFLTLMRG VWPASVRTPL DEETMRTHRE QNPCNMTFSS YQTAAEKGAV TD VRTTVAV VDDIILSSHR PVVIQSPLAV RQLSIALRRQ DVQRNFFLSR ETFLEVLRGH RLYLKDPESA LTVLDTAGDG SVD YLLYMN LLLPPLPPAR LMMLERLWEL FPKDTCGTAD VIELHKRFSA EDGEEQDAFL TAWDVRQALY RRFTFEEIVE WHTP LSAMF ELDNDFETML KKRWDFS

UniProtKB: EF-hand domain-containing protein

Macromolecule #11: EF-hand domain-containing protein

• Macromolecule: Name: EF-hand domain-containing protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 48.071531 KDa

Sequence

String:

MIGGQHKKER ISERLQNCQN QPKNRCYLPG TQLLTGGYST KTLQGNWSEE RADAGYYDGK AIVPTHLSKI WTTEYTVMTN HAMKRAQEQ APVFDQATLV DIVDRNHRAY PTHQPHLDPQ LPKLKEEAFK TTMRTSFNHP QEVKLREEAY VRPVIGNTPA A QARAIIMR FRRQLLISME GQSAFPGNVL RQVRLALERN DVVGNGVLNV EETFRGFTEA GVETSIPECV ALVRGLDMKG DN MLSIRKV MDEMRGEERD RRYSIIEGVY ELLKKLCSNG VVRLHHLVDL IDVDSMESVL NGTVSSADAL RAFTTQWDLP LEA HISFET FHTFFRDSSF ELKTDQEFEI LMRNVWHLSG GNGKNVNTSC RRLNVVHKDG RASVQEVKDD LDIKDDDPNL MERI LANLA TQGIKDVSSL TIIPKRR

UniProtKB: EF-hand domain-containing protein

Macromolecule #12: Cyclic nucleotide-binding domain-containing protein

• Macromolecule: Name: Cyclic nucleotide-binding domain-containing protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 37.578066 KDa

Sequence

String:

MSFDYAVLSP TEIIIYKERN APILKKVTNL LLRGGAFGYL NLEKMLHRST EKDSDDSKKG RRINPVTFKS VMVQCGVLLT PEEHKSLRA AYSDEGGFIV DQFLELVCPL RCLREEQISM LMGMYTDYDS APMIPLDVLR RTLEEALVAR SATPEAGESP V IASALVEL QTVFTPSLYP KGYVPPRDVL NFFAAILLNA VGDEESVVDW LSMVRFSPES PHEVAPALPP VLPLTQRRER GF DYYTDRD NKDEWIRGRE ERPPGEMYKR FLPGYAGHIP TYCSKFGRTF HTIEESAPTL TRPVQKLDPV PEDRYGPGVE LKP SRMSRH NFKLA

UniProtKB: Cyclic nucleotide-binding domain-containing protein

Macromolecule #13: TbMIP23

• Macromolecule: Name: TbMIP23 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 23.452754 KDa

Sequence

String:

MLMFHLNANR HLLQPPTKNR RGEVVSQTQR NRVCPAAIRA YRFLSDDISS QLNICCAVLQ VPFSGIPGAP PSTVVTKESV HPSSMLVVW NDTTAAEIAM SLLREVRGDV DSAFAAAESA TKAEGADEVV GAANPEDNLG ASRDDSATAV YVIKLSVGAV S ADGNATLS PIASVELRQV IRGEPLVFPS RRAGMPAVDV PISTLPYRLG DPIFFTCKRL R

UniProtKB: Uncharacterized protein

Macromolecule #14: FAP141

• Macromolecule: Name: FAP141 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 14.63142 KDa

Sequence

String:

MRYYPTGLDR DSHISGVRPG GGAPVSHDVN KRRDNLPDHV FASIREEVLM EERMRHVNAE ARARAQLIQA SGTHGKRMAG GSVGDDRRE LERVSSAEAV NARHRALLEL YSLEMEGWKE ELAERGLSIE M

UniProtKB: Uncharacterized protein

Macromolecule #15: EF-hand domain-containing protein

• Macromolecule: Name: EF-hand domain-containing protein / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 30.499936 KDa

Sequence

String:

MDVLIAKAVE RLGKKKLWVL VHVVDKLPES ERTVGGVRNA FRSINVFIND IDFAMLASQF GRRCGGVGIL RGVTTVTDES APFDVETFK RSFLPSLSPR RQYVVSLSLK RVPTDGSGFI TFDELMKVYD TARHPKLLLG WEAQALERMF LNDFWEAHSD G GITVEELT AYLVGISHKT VRDEDFELHC IRSFSLDRPK VSLEERMASA ASTDIRLKSI HGTKQHPLYQ ASSSDYGKGW ET VKYDGKF ACKYTFTKNL QSQCTMGPNT MNM

UniProtKB: EF-hand domain-containing protein

Macromolecule #16: Calpain-like cysteine peptidase, putative

• Macromolecule: Name: Calpain-like cysteine peptidase, putative / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 33.172227 KDa

Sequence

String:

MPRKALLGNW FEEEAYMRDR KRLLDSCDRG VVDAARETQR IIAKVKHHNS AYPMAEPHED GYLHFYAPLM LQNAATLGFL SLDLEDRTL RPTGWHVACS TAPAAGPALR NCFVLVPAPT GPTDMIPAPP DEQDIVHYGQ PFFIMTVPEL CDNPLSLLSE P KGPLSASK VTGKHQDVFF SPDGASAEAM WVADFANPDH REDMRDLPIK ADAVLVIRHN HTNTPLASSK AVFFNDFGPE NE VCCGRFV NNPGTPCGPM KDENYWTFVH SENGEGDVEK GGETTTPVAE NEVSVMTSTN GDKL

UniProtKB: Calpain-like cysteine peptidase, putative

Macromolecule #17: EF-hand domain-containing protein

• Macromolecule: Name: EF-hand domain-containing protein / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 61.839383 KDa

Sequence

String:

MNELKNPMRY SPGVLVGNWY EDMRVAEDKI KSYRSKMGHD ADILGSQRLE ESCVDADTHS MTMGDVIMLG KPLRLLNVAT EAVLAVDTA WTHPQRLPHQ FLLTATGNTA PRQRVEWVLM RAEDENNVGY TKQLKEENVL HYGQHIRIAN EAAHSEGFLY L HSSIRDVG QSGAQLAVAS LGTSKDNIFV VAKPGEKRDD IRYGAPVRVG DRFVLYHAAT NQPLRCIKKL QRTSFGFEYG MD CSFAGDN HSRSVAAVTT EPTNLFVVVA ANYGVTNTMS VSSLRNLTGR GNVGVLSESY EVDLSAIISL IREGVLYFGG RLG FRLLSK VLGVACNEQC VTPVRRQDIF HGISLMGVTI HPGELDVIFK KLDRVGNGFV VAQEFLRELR CELPQSRLQG VISA FQQLV IEGGGSVDYK DMLNLFVFNA CFHPDVEEGI ASREEIIFDF INCWPNMNST SSVTTDMFVA YYTDVSPAIE SDERF FKML KRCWKIPETD AYKSMKPCRS VTVFRSDNTS SIIYLPDSSV LNIKDLSSVR RFLTQCGVKD IKDIRLNM

UniProtKB: EF-hand domain-containing protein

Macromolecule #18: Calcium-binding protein, putative

• Macromolecule: Name: Calcium-binding protein, putative / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 41.708535 KDa

Sequence

String:

MPHSMDTTLF SDENRIDRGD SLLFHCVQLS QGGTDSHRYF FGCYFPRWRG FYMDEARELP GPLGYNVTRH FPAFPFDVYL KDDGEHFLT DDFQIGSIFT LGGPLNQRDD GQKRYKVVHC DDSQLRTRTG KTLAFIGNNV SGLLQQTHRV SGEAIDALKR I REAYIFNV GNGIPEVGIK AMGRHFRKVG SDGRRWMSYE GIVRFVKDSR NFNATLSFSD TQRTEEDVNT VATCIYNAFP KN EEECIDY DFFMDYVRGP MSQERKDAVW NIFRRMDYDR DGNLNIIDIQ ACYNTQDHPT CSVDHLFQSD KMLKGFLTIW DEN ERCGLV PYAEFLDYYN GVSAVLEDDK VFFDVLNNQW KLL

UniProtKB: Calcium-binding protein, putative

Macromolecule #19: EF-hand domain-containing protein

• Macromolecule: Name: EF-hand domain-containing protein / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 34.213496 KDa

Sequence

String:

MIYSDNNNPR EDSVFLRVKR AVRCGGRGPV TGPIQMVDFL RDFRCLEEEQ RASGRKGVTH KQFVKLMEQY GTKLREGDAA YLCKAFDDD NDGYINPERF VRHFTGLNQR RHNAVLRAWA SLPKDAKGRV RRNHLNERFS ETVTHGDVWG TFSSTVPREA Q ANNADDDS DDPTLCFEEF LAFYAAVSVE IPLDEKFELF LLREWCADSS RAPVMNSTLR EWGQGGDPLA IGKPLYVQDV LD RPLGLST KSYNYEHMKR VHPYIPPLPP LQLPYLSTMR KDYREFSTQE RALSNTLHGR

UniProtKB: EF-hand domain-containing protein

Macromolecule #20: Peptidyl-prolyl cis-trans isomerase

• Macromolecule: Name: Peptidyl-prolyl cis-trans isomerase / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO / EC number: peptidylprolyl isomerase
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 30.680295 KDa

Sequence

String:

MSLSSTTINC LGCTTTQGST ASGTQYVSHR HRLQRTSCWM ELACFNGFPE TPSLSGSLVQ QRHNLLDSVK SAGPPGPVFR VEFELFDDE SPKTCANFRN LCAGRTTSRK GETYWFQGVV PSYRGTYFHK IIPQFCAQGG DLTMRVDNGA NHLSSFGRGW F ADEYKRRR MNEVGLLAMA NNGPNSNGSQ FFITTSDSNE KALNGRHVCF GRVVQGLKEF MHEVAPFGDS AGYPSRFIVV TD CGEGPLP ATLGSTLPDK TPAADISEEG ARECGRVSVK SPSGG

UniProtKB: Peptidyl-prolyl cis-trans isomerase

Macromolecule #21: EF-hand domain-containing protein

• Macromolecule: Name: EF-hand domain-containing protein / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 36.100395 KDa

Sequence

String:

MVTPEQAALI EGAFRSMDRD GTGLVRLEDI FRVFDDSRHP RVRDGELAPA ATRDMLMHQF GATAQAHGGV SFDVFMRFHE RMAEDAAVA KVNDKELFLT DTIIGVWRLG TLLQPTLIRP LFPVNVRPSG LYATQYMSLV WVDEVAGPGS FVVHVVRDVV R PIFSRGDL PPQLRGMFAY PTELAGMKII EERLQIATQR WLDFVWEYEE GKHAAVPGII SARVDPDTLP QYLRDMIVEH DV AKAIPSL FFVPTSVAVN PMYKRSSEEY GYGVPEEVKR MSRWKDLTYS GQACGLIYHG RVGKFTRERL NAPVQDVASA MNI

UniProtKB: EF-hand domain-containing protein

Macromolecule #22: FAP107/MC11

• Macromolecule: Name: FAP107/MC11 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 28.127812 KDa

Sequence

String:

MRCHVMGPSG HKSEYHCATL LGNWQEERRS FGFVDPAREP LSYMTVYKDS FRGTKTDEEL ANAKPPSCFH ENALPELLFY HNEITNPPK NLLTVNELSY TNAKGGEEVN TLHDILHKEG VSKKRDKTIA IRRELGFIGY GAPHEGSLLA SKGATATPLD Y TMRCGTTT DQNFLESTRG GCYKPPVIPA IGRMQEKQRL LTTKNVTVDA TGEYLKDNID EYPATSSECW GMFMKFTDNM KG KKLRECY ED

UniProtKB: Uncharacterized protein

Macromolecule #23: T. brucei spp.-specific protein

• Macromolecule: Name: T. brucei spp.-specific protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 28.844326 KDa

Sequence

String:

MSHLLRSRKP YVPIDTCSPA GTWIEEKHYN KERHKDGATC SRKVLCGNWQ EEKVLEDDLL AQQPVPSDPK GQMDFTSTTV LRASLDAIR PGKNDRFTGG YSSTPYVTSD ARNPHSRFLQ TTNREDYCEK NADLRTLRRP DLGARSRVLL QVALNAAREE K NATEAERK QLTNVGMDGN NVYLSTYQKV HCLPEELPVV DSLCNDYLSD EPITLYTGNP ETGCTMTVHG KTPVEPVEGR SR FGRHTYF TERKYCL

UniProtKB: T. brucei spp.-specific protein

Macromolecule #24: FAP95/MC6

• Macromolecule: Name: FAP95/MC6 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 20.151945 KDa

Sequence

String:

MHTGDKRVLP CFDNHYNSGV LVDNWFDARI NEAHGKFTIA VDGQSPLPSS VYKADYSTPC VTAVRPMLRQ RGLGKSLIFG SGLPTVDEV VATSKDYGAT QRYLIDSRYG KPKHHCKPLN TLSGVDRFAL TGKLSKPVNQ PTVDDAMVDR FRTTKSAMDV T ILNHHVQR QLSAGRNCGA RTQM

UniProtKB: Uncharacterized protein

Macromolecule #25: FAP129

• Macromolecule: Name: FAP129 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 42.654492 KDa

Sequence

String:

MITLKTAVNT RAPLRERAVP LDRDKRLVPS QSSLHHGIYT TFDEAYGSAP ARTDPSQGNH YLTLLRSVRC GANKTNEATA DTKTTVDDK ACGGEMKPRG HLEGTMKSSV ASLFDGVATR HSPSEWITEQ QASSQSVRPV AQRDFYSTAR RVERIDDDMR S GLVGNTQR TVDIMRKRAT SPTLCPNPDV FPVFPAQRRL LDTDADGRCA RSCLDIVDCQ RLAPPSENHL GFEYAPLDRL AP KLPVSPA LAVQQRLITD MSSSMPLFAG TAKVQKYAIP RYAGHVPSFP RNVDALHGND TCPLRKWSKS YVTLATVGCG TDA GRRNKN PLVRNRSGTK APETKPMKPK TSEVIKMTVE GSMLQTTLTQ LTDAEQTLNT RVDKKPRKLF

UniProtKB: Uncharacterized protein

Macromolecule #26: T. brucei spp.-specific protein

• Macromolecule: Name: T. brucei spp.-specific protein / type: protein_or_peptide / ID: 26 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 46.540539 KDa

Sequence

String:

MEEGTYAGQL GTQNLDSVVE TDETNFLHER LSSKISNGFA SSAYWGATGE LPPREPDDVA GKKPHCFEME LNRKLVPFPE DKTSLPRIL DYSHVGLHRL RDGAEDPPKL NEAQLRALEA GGSASGDNTL KRRGLPLLER TTTQGRTIGK GILGPEALNA L REGNANIS AAEANREQLK SKPFTSADPN AYRPTSWDYC DMTGIDPSSY WVTALDQESV GMPAVYKSRY NLVEKEGPVR RE RTTLMLE RGKTVDKKQL RDTLDGINAE AVPQGYKTWS AGHWMSTTHD AHAPYDIGGA TEINKRNATV PLPRTYHTLT PVH EETVLS QTQRHLNRHN GKWATEYSVS YKDSFDEAEV NKAYSKRSIF DIRDGAYTMH PYAHHPRDDT ATGENYTPAQ IVPG QYTSI ARQPLHARNA IK

UniProtKB: T. brucei spp.-specific protein

Macromolecule #27: FAP21

• Macromolecule: Name: FAP21 / type: protein_or_peptide / ID: 27 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 46.442445 KDa

Sequence

String:

MSSPMCSVHS NPLGGSRSRL HIAPQIIPAG RQGSRDGTTV RELTSNHYSS GRVTPELQRT YHRFGEVGCT RRHYGRARDP PIDETFRHG IRTEAGEGAR GCLQPETGGR MMALMEQQLE RAYLSNVRRP LGKVPAAMYD VQVPHSGFGI PSEKSESVKT L LYAGRDSG TVHPVGECKN RGYDWERAGI NPMHHRFGWC EQRGEATAGE VMCETKLVTR LLPKVVTDVR KLTKQEVGKG LP PPWDTKY FDDTLESRTI RRNGRGEGDA VRQLLSSWMH HPFPPKRQLV AEEEDASLKG TGSLEGSRKE GLEKDALRSR FLC TYRRGG RYNSADHTRL DDDVRAPHVL YPCHYVQMGV NSSRFAGGCT LENVRDLCKS VGMDLAENQM QEVFNHVAVD GVCG IEQFK NKAVEMGYLS

UniProtKB: Uncharacterized protein

Macromolecule #28: Flagellar associated protein

• Macromolecule: Name: Flagellar associated protein / type: protein_or_peptide / ID: 28 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 45.935605 KDa

Sequence

String:

MFGGSKSDLI MSPPPHPKAN MLDLYMTRYD YDYNRDIDPS GQGEKKSNFT VSGEVRNRPI FRAGAQTGGE GTTYRSHYVK HDVEGKDFR DILQSCCRAG EPETAFVHGR STHVMGCSTG DGVPPNTTYK TDYVYNGRSI GGEPQLYAGA KATAFSPNLL A IPPTEEEL RKMAEVAPKI TSIESLAPKP KPSLEGLPAN KEDLLASRRP QLTTGGHPTD YYCTSWVYGD KSLVYPSQLP CG LTNSQNG HLIGTIQNKA ELLALLAGRK KAAAKATNPM PDTTNPIAID KAAQPYCGVT RRLENEGHVK MSMYKSNYID QAV LPELPD AAVQAGNHDP NDESDTTNRR AATTNAGTLT KRMHRLGTLR NSHGYVHKQR ALDSDIDLQT WRRMRIIEKR IDVD KADPH RHKLNHRAHQ

UniProtKB: Flagellar associated protein

Macromolecule #29: TbRib26b

• Macromolecule: Name: TbRib26b / type: protein_or_peptide / ID: 29 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 26.226729 KDa

Sequence

String:

MSFVPASQTA SVDRDVFINH GGAYWINPVP GGTFAPRSTY TSSKKGDLID PFGNPKVRDV PDLFGRSRYS RVPIGSSGDG AIRETSGAE GDGNGGVDAV EGVKAGTHGN DGGLQLGSGL AGTVGDHGQQ GETIGTCINS AEGKQPFSTG DIMDKQLPLD T ANPERRIA HPGNHTDRIP PTGGSEYNGT VTEPWCAPAA VDNTYLRLPL IYESTTHYQQ RTGRYYTEDQ APPHGMSLPP KC SLPTREW

UniProtKB: Uncharacterized protein

Macromolecule #30: PACRGA

• Macromolecule: Name: PACRGA / type: protein_or_peptide / ID: 30 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 33.834922 KDa

Sequence

String:

MSYEIQPILK GTKRDPATRK YNRAAGKGPF GAFPPGYAPK QEKPSIPIEG PVAVQGVRFA YKGTVQRTGG TTTSLYKGRQ QHESAVAFT TNGAGDSKPP KAGAFKRRLI PPTEFRRYYD RGDLPLSVAH GNRPTIDWKV DVERLDYHHY LPIFFDGIRE T EEPYMFLA RQGCLDLLKR GGPKILPTIP QLIIPIKTAL NTRHPEIICA TLRILQQLIV SGDLIGEALV PYYRQILPMF NL FKSRHKN RARGDAIDFG QRKRDDVGDL VIETLQLLEV HGGDDAYINI KYMVPTYESC IFS

UniProtKB: Uncharacterized protein

Macromolecule #31: PACRGB

• Macromolecule: Name: PACRGB / type: protein_or_peptide / ID: 31 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 35.401648 KDa

Sequence

String:

MAFSRKERKQ LLRDRDLVPQ LAESPFGPFP PGYGIKNDIT VTKKGCVVRG DPATKPASAD VQKRGKQDTE SDQRPKQILV GHTGTGGMT FSTFKNNGFR QSLKIGCFGD PKGPEKAGAY RKRVIPPTEF RNYYVRGDLP LTIQQGASRT LSWRLNVSSL D YHHFLPIF FDGLRELEEP FIFVALQGCY DLLERGGDKI LPTIPQLIVP IKTALNTRHP TVIINVLKVL QKLVESADFV GQ ALVPYYR QILPVFNLYK NRNKNIGDRI DYSQTKRQNI SDLVNETLHL LEQYGGEDAY INIKYMVPTY ESCLM

UniProtKB: Uncharacterized protein

Macromolecule #32: Cilia- and flagella-associated protein 20

• Macromolecule: Name: Cilia- and flagella-associated protein 20 / type: protein_or_peptide / ID: 32 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 34.593465 KDa

Sequence

String:

MFHNSYQSGF LSILYSIGSR PLQIWDTKVR NGHVKRITDE DIQSSVLEVV GNNVSTCYIT CPVDPKKTLG IKLPFLVMII KNLKRYFSF EVTILDDKGI RRRFRASNYQ TTTRVKPFTC TMPMRLDEGW NQIQFNLADF TRRAYGTNYL ETLRVQLHAN C RVRRLYFS DRLYSEEELP PEFKLFLPIA QQQQQQQQQQ QQQQHMQKQA PPEPQQQQHH QQQQVHSEAH QQQQAPPEAQ HQ QAHSEAH QQQQYQQQVQ HSQFQDAAPP SQQQTHEEMP QGEEEEQQQQ TSPVEGA

UniProtKB: CFA20 domain-containing protein

Macromolecule #33: Cilia- and flagella-associated protein 52

• Macromolecule: Name: Cilia- and flagella-associated protein 52 / type: protein_or_peptide / ID: 33 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 67.770891 KDa

Sequence

String:

MAAKEYETTP RLTLDGVIGY SGRIPNSILA HPNGEHLIYA LGACIVIQKI SDRSSSDFLY GHNDKISYLA VSASGRYIAS GQMAHPGFQ ADVCIFDFEQ RRMIHRMLLH KVKVQALAFS SDERYLASIG GIDDKAVVVW DVATGRPLCG APAHHTESKT V VFYNNSSD KLITAGIGSL RVWTIDGKDR KMTAEDVNVG NTRRCITSVV VEATDRYAYC GTTTGYVMCV LLERDALAYK MS GPQQMLS GGITSMVLDP SGDVLVGSGS GEVALLSKIN LTILKTVTVQ GSVTGICTVP HGFLVGTMSS NVYLVEGGNF RAE LRLTCH SDTINDVVFP EGLSALFATC CGPDIRVWNA ASSAELLRIE IAGLTCNCIQ FSKDGSMIVS GWDDGKLRAF GPQS GKLIF AVNDAHKKEG LKSANGVTGV TAVCTDNSSE RIISGGADGL VRVWQVRETH CTLEASLSEH KGIVNAIAIT RDNTQ CVSA SDDGSCIVWD LVRHVRRDVI YSQTRFRAVA YYVDESQLLT TGTNKNITWW DSVDCGAIRE VPGSKTAEVN SLSLST DGR FFVSGGADRI VKVWGYDEGS CAAVGLAHSC NITKVRVSPD GKKIVSVGDE GAIMIWSVCD LEFKTL

UniProtKB: Cilia- and flagella-associated protein 52

Macromolecule #34: Enkurin domain-containing protein

• Macromolecule: Name: Enkurin domain-containing protein / type: protein_or_peptide / ID: 34 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 30.26426 KDa

Sequence

String:

MSGESVFNLI EPDVESVQPQ PLRKVKLGKG TAAPPTASTF GFHGTSAVVA NVGGENTEPS VHPAKKPTGT FGREASSSVN PSKFLKKNE GVGTASRGVP IVDATKFVKA EKDASKRKDD VPNRYDKPVM GVKTEKNYVV ANAVENVLAL PTKCIPTPMN R AVDRADFG KVPKYLKEVK ADIEERHALV ERLKAAKREA EERWSELSGE ELEQLKQGLQ RRWDSLNKDY QSRGFSKLQT PS QKAQHEA VGKELNAVEF AMQKLSRAHV FVYDDRN

UniProtKB: Enkurin domain-containing protein

Macromolecule #35: Enkurin domain-containing protein

• Macromolecule: Name: Enkurin domain-containing protein / type: protein_or_peptide / ID: 35 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 42.281645 KDa

Sequence

String:

MSGENIKNLL APQPTVDQGP YSGKYEKRMV DLPKPTYSTF YNKNKEFDST HPRYFKQRDA VIGPIVNDTV DPKNFLRTGQ GIKHIVPPV PHKKQHRKDF LDCGDIRKRT QGAMEPADQQ QKESSGMGGM GSLANTNGVG GQAGDGIREH DMSSNDPERK G EQANGGDT GLRGTGNVES AVGSCDNKNN FQGAATGDNG RCHLNSLDRS RCKKDFVTSN IIDVDNMVPK RRKDQPEDPT RR KTFGRVP GYIGRVKEVI EKERMQLKAI GEARTNNRLE RVGKFVYRLD EQERLNMISK LRAKLTEKGA ELNRMPFAKD TYI QMKRKS DLEKRIKEIE SSLEKLEKDA VFIYSDDPRV VHWTKDAAFE EARLFASD

UniProtKB: Enkurin domain-containing protein

Macromolecule #36: MC4

• Macromolecule: Name: MC4 / type: protein_or_peptide / ID: 36 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 40.548527 KDa

Sequence

String:

MFSTTTGSFC GPSLSQPVSY SLLVLPPKKE LRKKGYNMTD INTTSTRVHP LARWQTHVLK HGATYRDALD AVEEANTKHW GFLKARIQF SCGSFESFVR TNPNDPSTLK GVSTYDPNGV FHKETLDCTL KNRSTLLPRL RAIVDGRGHH LSGSTPPARS F HPQVLYKN CPPPVLSQAG YDFTPMSHNA FLLRTNDHPQ GVRDVKSDFM KGSCDYRPRA YLRDEVSGGV NSRHCHCAEV YQ VGDYTMD LARGAEIDHR NRTVNFEYTK KGTLKSGSNI VGKRHARVPR FPCDHSVKGA CADAGMDTRD GGELPLVSGE AVA EGAEQG GATTSPALRS PRRHCGAGNG KGSSDSPVGR SQEREKQVQV LVD

UniProtKB: Uncharacterized protein

Macromolecule #37: MC5

• Macromolecule: Name: MC5 / type: protein_or_peptide / ID: 37 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 30.760701 KDa

Sequence

String:

MSTIYNAVDA STLNDRCRTD LLDTVAQLGR HCETLPKKAV TTADAPLPSC TFNTTVSRSQ KEINCSNEAA LDKYIEEKGA TRWRTTQAL ASTYFKDHKP KFHGALRTQD CFRDTRDITQ LSSKEKDFVH RYAEAKMKKS IPREESDDVA KKVATEAYTE A FRKFKGRD PTEKELLEVE RLYFAYADDG AVGVVQKPHF HGPHMHVSIC NRKYVTCPNQ VYRASLPAVR NARSRFPGPI PV WTSTKNL HAVASNDTYR PGVHNVSKFG ETSSK

UniProtKB: Uncharacterized protein

Macromolecule #38: CCDC81 HU domain-containing protein

• Macromolecule: Name: CCDC81 HU domain-containing protein / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 29.888291 KDa

Sequence

String:

MEPSKTSTRR NPDQCYACYS EIANIVAQMG GLYCHQSRNA GPQLLCRIWR TLCESVEETL VRKRSCIIPD FLHASIKVQR VSPFDGEIN LYKPQMVLLP DFLSKFHVQN VLTFRDAHYH ATVPNIISFD EIAAIVGTDR HIVKAAVSES VREIGKYLDR N KCAVLSID VGVAFLEFRG REYRIKWADT FLERLKREVG PRSFVEPYIP PTMAPDARGC RFQSECLTKE SLHGSLNDTL QR ESRTQVV EMCGSQNKQF TLSPS

UniProtKB: CCDC81 HU domain-containing protein

Macromolecule #39: Cilia- and flagella-associated protein 45

• Macromolecule: Name: Cilia- and flagella-associated protein 45 / type: protein_or_peptide / ID: 39 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 58.198273 KDa

Sequence

String:

MIGMMARSGA GVFPPRRPGQ TDGDLRKELN DRNAPRDSTI LTRTELDIIR EMISGKNIMT TLTRSAVRTR SVEAEEHKRR MQQYDEEQR LCGGNDKPLE QIEEEQQRRL NLERAKTLLD EQYDEVKAMN QIVDEARCIA VRNAQIRERE LRKEEEMEYE R KMEEMMTA EAEKAAKLYN EREEQQVVAR KKTLAVIKAQ LEQHDVERVR KLELLQHERE AMTRHLELLR EEAQAEKLQQ QE KERRIME AVALANAQQI SLKKRQQELD EEEDRRIAEF IKRKQERDRL YAEEQQRIRD EKEREVARLR AEQQRAQNTQ ALL DDIRAQ RAQEEYARDM RRKEKERKER EAAVLQDLAQ MREKQIEERK RMKAEERRLE EEEVERINAV QKVALEQERE RKMW ARKQH EENSLAVLKQ IMDVEERRRR ERQEYVAEGN SIMMQIRERE AAIEAIRQRK LKELEELGVP EEYCQALQKK MKVKV ARR

UniProtKB: Cilia- and flagella-associated protein 45

Macromolecule #40: Trichohyalin-plectin-homology domain-containing protein

• Macromolecule: Name: Trichohyalin-plectin-homology domain-containing protein; type: protein_or_peptide / ID: 40 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 61.310469 KDa

Sequence

String:

MVGQTQSTKV LSSSTSQVGR ARVDPKRRYG FANAVAKGSL ARRVGVMSES ELCNIRHILT ADEDSYNAYR RHVDEQRAEA SKARVADWP DTLQAKQEAF LRLREQEKKE EERRKAMLIE LSGQHQEEER KQKQAHMAMK LLQEDPRSHH VRSLILLDEA I KDRDAQLA VKAQVKKAEE EQQKREQEIL MSGAHDHILK EQQEKYDRIA REVDLKNNHL QQMMFQIAER KKLKALSKDD AI EAKRAAE EEEQENLEEF MDMRKKMAEV DKYNRSIAKP PLSKHGRLLE RIKRDELEEK EHSRQEQALE EAKKDIKARI ERK REYFER AKEISHKAFE AEHRATQQIA QTQDVFEKRW TDMVGRMAAD DDARKQQMVE ERRRKAEELR RRTMGEVEPE VLPE NIRKA QTHRAGFMDD EEARAYQLEM RKHPERVRME QRLEAERLRR EAELLQHIHK LQAEERKENE RREEAMELEA QRLLE EAVK EDEERYRAYV ESQLPANMNP YLRQKAMELH V

UniProtKB: Trichohyalin-plectin-homology domain-containing protein

Macromolecule #41: Trichohyalin-plectin-homology domain-containing protein

• Macromolecule: Name: Trichohyalin-plectin-homology domain-containing protein; type: protein_or_peptide / ID: 41 / Number of copies: 9 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 41.736418 KDa

Sequence

String:

MPLPPVEDAP NSMARRHYLV ERNRLRVKKY EPTRQAFEEE TVKLSKQRVE QRVAMLNSWK SSVPLHTDTT RPLPGAARRQ KEKDEPAAK HINLQILDED AALKRERRAL LRADILQQKK DREEYLAKWR ANEKAYDSAL LATNAEFARQ MQEQERQAAV A TKQYMDMM RASNLKELEA KRAKQREKEE ADVAALRTMQ ENLRLKMEAD ERRAKDMKRL MQIENEENHS LFKKKQAEDK AR EDAWIRT MMEHNAALAE RERREAEQKR QQFKADFEDT IAKQKEFRRT HDYDEPQELI RKRNEEAAAS AVLIRQEERL RNN EQRKQY REELMKQMRE KYEWQLSHLD GV

UniProtKB: Trichohyalin-plectin-homology domain-containing protein

Macromolecule #42: STOP axonemal protein

• Macromolecule: Name: STOP axonemal protein / type: protein_or_peptide / ID: 42 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 30.09023 KDa

Sequence

String:

MTTLHTISSL DYGDGRTCVC RICVCGKHRC TDPKLPFFGE TTYRDEYVPK STVRDIGRAR PSTVFPMKAD PGHFKTTKQE AWEPLEGKI VRPAESFKPR TVLGEPLPFN ATTTHRNDFP GHLPDHHRAR YKQEPLPKLK GTYETTNQAM QEPLNKHLEA G EMPKPPQP FRGDATLLRS LPFDAMATYT VDYPPKEALQ QPRPPQKQPF ESMPDVRDFG TTHSESYKVP PHRVRRVCPA SQ LAPRPPS VDGHYKLSVY AIPSGEIAD

UniProtKB: STOP axonemal protein

Macromolecule #43: MC8

• Macromolecule: Name: MC8 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 31.647955 KDa

Sequence

String:

MFSRRRKVAV VAERPSLTWH LMREEYHPIF VTDSSERIEY WYNKLATQHQ RENFRRICKE ISQQDPERGL PKMSALYSLQ YCICEADSM IRHYGFMFSV LGKKLARLWI MHDASHRDKN TFTELFTGLQ TDFIPKSVMK TDFQPPEQGD YEGIDRSHFL S SIDWFNPE SKRNLPRVDI QKFGNKIAST RASLPVKKPK NQFDGVSPLA VLGYTGDLDT EIEKLREKQQ KSEEAERYYV ED GCTVYVA GRKENRRAAT LSKNRVIATF YGD

UniProtKB: Uncharacterized protein

Macromolecule #44: MC3

• Macromolecule: Name: MC3 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 37.3275 KDa

Sequence

String:

MPLRTDYARY PRDVREKQNV TRPAGERRLA DLHLTAYPSV LNSNALAAAD SSETPPKPVA QNKPYMEKPP VELMTTNQLT ALLSKMAKE EALKDTTKGT PLGGVECTEL DRKTTYRLDY CNKDVPGFHT RQRVIPDYMK NWTETEKDDI REARKYPYLS I PQSVLNCR PTTYRRDYIP TAQPNEYRTL SHKTNLQDMP PSFAYSTYAL DDPRRIIKYD EVVAACSKPK CMLWKSYSPR DS FRAKTGV AKDVAEELRL LQELRGPSNR SSESSSVAGK QRESSVECKK PILLPGLRGM GYRMAPGDGK EYIWLPRDHF CHR IVYDY

UniProtKB: Uncharacterized protein

Macromolecule #45: FAP90

• Macromolecule: Name: FAP90 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 21.434402 KDa

Sequence

String:

MPKKKPPCPS VTPQLYEKSH KPSVTVGMGM KGSAASTACW RDQVDKEEQL HAIWAKRYDR RSNARLVESL KRTLQREKDS AEQYRKDND ALSTILFSKD VSDAGRLTTV YLKARNRLAP QEKYLRAQTA AQEVGWGIPE AMRGGDSVEF TGTPALPRLT K ASAMAYRR PDDEDHAELF GYTLMCSFCG GKP

UniProtKB: Uncharacterized protein

Macromolecule #46: PBP36

• Macromolecule: Name: PBP36 / type: protein_or_peptide / ID: 46 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 36.147027 KDa

Sequence

String:

MTEGRFETIH NLRPKNWDGR RHWTNWHHLY DCEKDHLARE SCPFHDLRSG GQFQYENWGG GEFKPLIPPN HLNNRPCGDR MDFSKGHGT QIGGLGDIPL DVEGGKPTQH NKHPGKTVMF TRKDPLKRGL FSSYPYIPEA STGAASAGGP AGPNIKTGKV N VFGQAPEW IADPYDGKTD RGRIFHFKAG PLNYRPDDRL AWMPEGEPER RKKRIHGVFR AGKPCGIIND VEWVPDPLQE AK VKKQVRP FRTWHTRTKW SMPTHAPWST GKITAEPFRG PNLNITQSGL PCLDTFKRVN MTQTIGKEAA LAPVELPGPA RLK

UniProtKB: Uncharacterized protein

Macromolecule #47: Enkurin domain-containing protein

• Macromolecule: Name: Enkurin domain-containing protein / type: protein_or_peptide / ID: 47 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 31.393762 KDa

Sequence

String:

MIGGAVLNPH KPATEKPSTE TPQRPSRLPT AAAAQHDKKY HHCDCYGHPL GLPNYKVLAQ PRTNNYYNAT GGCPCIPRSA AHPGSWRNK REQLRSTANH FPFGCTKKRP GTTCCVHAGE TYGWLPGPHK VLAKNCAASG LCDTFGNMAF KKPIRSQDKD S DVSSVTTQ KLAKLEELIL REHGERRRIE MDVDELEDIK KRGAENDYKV DYENEKKRKQ AMAASEGQLK ELLREVRGIV NR PLNQTNI DILRGIVDRQ EQFMQLRSLR AHEDFPPIIH P

UniProtKB: Enkurin domain-containing protein

Macromolecule #48: Enkurin domain-containing protein

• Macromolecule: Name: Enkurin domain-containing protein / type: protein_or_peptide / ID: 48 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 30.82373 KDa

Sequence

String:

MAQTSNNVTH SDKYHVCECY GHTQQLPNYK KLTNPRTLQG FYAKQKVSAC PCIPNKREKN FNTTYCSENP AKRNVAFPEE TYRDFEPGK HRVHKAASVP GDGATLRDPV TRARLVFPNA CNYGGTVAKH TFPAIGQPRR LTPGERKYEQ MYDRKEFLKA V LRDEICHR AEAEKQLEEL ENETGLRGSR HARLLAEAAA AAREAQLSED HEEPKTVGEQ YDAVMDELRF VTRQPVGSKL NI IRMYYTL EEQERMRNFA RSFGSQKTSG RV

UniProtKB: Enkurin domain-containing protein

Macromolecule #49: PON3

• Macromolecule: Name: PON3 / type: protein_or_peptide / ID: 49 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 46.311688 KDa

Sequence

String:

MLREQCKDFC RCSYCEKKRS FNGTQPFNIT LPHSNCHTKD ELYVKALNDT LRGKSLKASM KNSYKFEPDE MLLSTRGNMA RAALTVPVR RRDRLQELLI LENRERLRTE QELRRERLRS GLTLPDEDVR LHDQSVGTDG QELVVEGKSA SAEQQPVCSA D EDLREALQ TIKNVTDGHK ASGGPRPLSV RHIEMLRELV RKQDKKTKKM VAECPEDEHL CGHCFAVNAQ GKHECQVPPA DV LRDSLLP SVRGKQQRFN FSSHPYGSGV VWLPLNTAGD RTALKEGASQ ASGVLKDSYA NSNAGAQGNV EDVDAFDAQD ARS DRTNGS GSTGSGEAAA GEPNAQKGGT PPSTNKADES GKQTKEDESA ALKSGTALWR TTNQETSMQM NRYLEFKKLH DAAA QVYAE SAVRRPASTL PAAAN

UniProtKB: Uncharacterized protein

Macromolecule #50: PON4

• Macromolecule: Name: PON4 / type: protein_or_peptide / ID: 50 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 37.083625 KDa

Sequence

String:

MSVKLPHVSV QYQSHQHCQC DFCNSRRNLN AKKSALMTTV SHLREQQALH NCCCQCHDSL SQRNYDRKPQ EPSFELDDPE HHPNQTELN AKMGRNGLQH GGEYTIPPGV RAAQQCLLNG SNGMAKTREE QEKDAMDDLD RLERLLVLEH KSRVKAAADA E NFETLKRT GKGPRPIPFD APEEQVRVKS ATPPQGTIVM PGTVREAYDA AHRCSVTPPP HCEDSHRLQH VIEKVRTVAR DP ANRDNAA ALGAVLHRQC GAGTPPVSRE GVATPPIEGM KTIGTLNTYG PRPEGAGVVW CRGLNAVVDD GIGTSAALGN TDN RDLWKA TLLKNQPTTT N

UniProtKB: Uncharacterized protein

Macromolecule #51: MC7

• Macromolecule: Name: MC7 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 37.334016 KDa

Sequence

String:

MVRWGGPRFE FYFTRDFLNH EYLIKKEEAQ RAAFIHSKRE HERAKEDARF AEAEQKKAQL QEEAQRLMSA EVEEAARRAA AEAAAQNTA HARRLRRLKE LAVPVIRGSS AHSAGSHGCV MYPTPKGVPL PMGRDRAPYT PGVDRHRIVP PPPEPGAHKP W QGSGTAWM TDKNNDWQKQ YYFTKSKRPA ETLRERRACI DKMERCHTNE GAAKKPPVNQ DESPANDQRE IPTFGTERQH DE MGSESES ERPLSIFSHG SSNAPIRALG TQHMGDISTK EEVREFLRRY PTGVVPPRDR RAAPPNFNGM NLTELTSSLI KMQ KCNLCG GHP

UniProtKB: Uncharacterized protein

Macromolecule #52: FAP96C/MC15

• Macromolecule: Name: FAP96C/MC15 / type: protein_or_peptide / ID: 52 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 39.428738 KDa

Sequence

String:

MTHLSAFGGL FSDPPLVSTD IDPKKTDPYD KMDTIPSRYL GKGMSIGRCP SGNGPEVFFD KKFLTLASAE QNNNKSVGPF EDATTRMHR ERREAKKHNI SNKDFLPPSF PKSINGPGGY SGCFQERPYE FIMPGEEGGP KSKKSKRKKK ASSSEEDSKG K ELLPNIKT NPSKKGTYGY VGILLDNPKY NDDWRKEHEK LEAAEAKKRK KLAPPKPLGP LFRTPGVIHD YLDELPATGV SG VYHFEPA DEKPSKRKRK NKQEAAGPTF VQERAMTFVW TRSGNEGNID PFPNTWIDVA ALEEEEKKKN RRRRRAKAKD TYV PGPPKG ATGVWKPNTF ECTSVVQSCL RRFY

UniProtKB: Uncharacterized protein

Macromolecule #53: FAP96B

• Macromolecule: Name: FAP96B / type: protein_or_peptide / ID: 53 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 33.435266 KDa

Sequence

String:

MVFSIPSYLC LGDRPKKVVD ERLHGVNFLT SRPKTGHYPD ALFDKEFRYV YNGDRYPDPE TMARREQMEN RKRYITATGF ISVFRPKKG EGLGSNYGLL QQEPYIHMPD HPQTRGPQPF PKVKPRQIYT SPSKAGSYGT PGLAITDIGN EYIATIYDQE R INAKKERD VWRQRMPPVP FKPVGRRGYT FDEGPATGVS MCYIMTCPFR EKRVQPVMKH FIIDKMWLPA GYIPDRPPPV EY WEDPYNG FDPRVDPKSR LKKSEDSIFR TGFRGDNFFY TRSIVFRRL

UniProtKB: Uncharacterized protein

Macromolecule #54: MOP23A

• Macromolecule: Name: MOP23A / type: protein_or_peptide / ID: 54 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 23.471566 KDa

Sequence

String:

MADNEEVPPS GPRMTREETD ELVRRLYDQQ MERAARREEE RQRQLARPFC SSRRIKKDEE ENLVRRIYDV QRERFQQSKE ERERRLTLE LQSKDKKLPE SEIQDQVDRI YNQEVAKSKA RREELQKRYL PEVPPKTIGK KQLKESVERL FRVDYVKRDE E LFKKHVYP YDPPTTKISR TDVEAMANRL SRRGS

UniProtKB: Uncharacterized protein

Macromolecule #55: MOP23B

• Macromolecule: Name: MOP23B / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 22.494564 KDa

Sequence

String:

MEDEPTQILL TREQLERSVE RLSKPHSREV NLKPLCKSVR LPQEARERSI KHLYNDSMEH KERRLREIEQ SLNAEIEKYH AGKPKLDSA DTEGLVSRLY NESIQRKNDN LRQLFEHQVS LSRPKEKKLK KAEQQEFVTR LYQGGMEHNR KKHIALFEEH V LAREPKMA QRSPEDLEIA CSKLTSGKSV TDD

UniProtKB: Uncharacterized protein

Macromolecule #56: MOP23C

• Macromolecule: Name: MOP23C / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 19.407205 KDa

Sequence

String:

MEQDSHPRIG LMLTEGQFEA LVTRLHDKSV EHKAETLRQL DARFYPTAPP KRLPKEAIES SVVRQVDHEM NRRRAARENL EIQEERKTL SKKISSADVE SSVERLYTET LARKKANMEE SRKRYLYAGP DMVKKNAKEI QEYVGRLAVP KKKEFTIEEV N KVYDLV

UniProtKB: Uncharacterized protein

Macromolecule #57: KIAA1430 homologue

• Macromolecule: Name: KIAA1430 homologue / type: protein_or_peptide / ID: 57 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 19.570469 KDa

Sequence

String:

MSVAPKRTAE LLWLEQQRAR QYEQHRKRVE QQKPCVDNKT PRNLSLSNKR ALMEQERRKC IDEENRRLVV NMSAIMERGG GIDNKEPWR RTNGPRDAEI RRRREQQKLA EENLKLLHRL ENVKPVYRLE KWEMERDENE ILVDRISRYP YIPMNRRKGV G E

UniProtKB: KIAA1430 homologue

Macromolecule #58: Starmaker

• Macromolecule: Name: Starmaker / type: protein_or_peptide / ID: 58 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 95.91468 KDa

Sequence

String:

MSKSLPPLES SSRGGEAARV GVYASHGPAA TNALCVVTPR STVNSNLRPQ SAQRFLETEF TLGPLQDQSL PPYNDLEDPY LAPYWARRE MLIRETAERR EEMRRQKRLE QHRREVARRR FEERRQRELE ELACRTGFAT QRRVEAEELT SPKETTSGRK E VAKKRQRT VSSRATGSKK RAVATVPKPP KQKRPDTVGQ RGGRPGACSS QGKGKTSSPP KNILLKTIRE TLNSDSSRPA TP QKGKGAA SPVGGRKSGG GLTESSKHGA VTEDEAPISS SVAEDSATGK GSTSSVAQPS EAKRESRPST ASPQPASTEQ STS KEILKD GGNSRPASAA RPSSASASAS QKAMQQDTTD AEDGKDTQSD RSRPSSRKSE RSGREGREKT DDEPTADTTK NDYG DEFEA SESGKKDPDT EDQKDTESER SRSSSKKPEE LTKDDADKTD DEPTADTTKN DYGDEFEASE SGKKDPDTED QKDTE SERS RSSSKKPEDD EQKDEYTEEL DKSQTSEKKD TEAEDQKDTE SERSRSSSKK PEDDEQKDEY TEELDKSQTS EKKDTE AED QKDTESERSR SSSKKPEDDE QKDEYTEELD KSQTSEKKDT ESEQSRSSSK KPEDDEQKDE YTEELDKSQT SEKKDTE AE DQKDTESERS RSSSKKPEDD EQKDEYTEEL DKSQTSEKKD TEAEDQKDTE SERSRSSSKK PEDDEQKDEY TEELDKSQ T SEKKDTESEQ SRSSSKKSES SAKLEGDKFE YGDELERSPS SEKGEAVQQV VEDEHVNDQD VDELDSDAVK SEAVDEASG ADAAKDSADG ASSKESSSKR TSRSSSSVSK GRKNASSSSS SIGSEELSGN DVARASGTGE DEIEDEA

UniProtKB: Starmaker

Macromolecule #59: Tubulin alpha chain

• Macromolecule: Name: Tubulin alpha chain / type: protein_or_peptide / ID: 59 / Number of copies: 145 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 49.840094 KDa

Sequence

String:

MREAICIHIG QAGCQVGNAC WELFCLEHGI QPDGAMPSDK TIGVEDDAFN TFFSETGAGK HVPRAVFLDL EPTVVDEVRT GTYRQLFHP EQLISGKEDA ANNYARGHYT IGKEIVDLCL DRIRKLADNC TGLQGFLVYH AVGGGTGSGL GALLLERLSV D YGKKSKLG YTVYPSPQVS TAVVEPYNSV LSTHSLLEHT DVAAMLDNEA IYDLTRRNLD IERPTYTNLN RLIGQVVSSL TA SLRFDGA LNVDLTEFQT NLVPYPRIHF VLTSYAPVIS AEKAYHEQLS VSEISNAVFE PASMMTKCDP RHGKYMACCL MYR GDVVPK DVNAAVATIK TKRTIQFVDW SPTGFKCGIN YQPPTVVPGG DLAKVQRAVC MIANSTAIAE VFARIDHKFD LMYS KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GAESADMDGE EDVEEY

UniProtKB: Tubulin alpha chain

Macromolecule #60: Tubulin beta chain

• Macromolecule: Name: Tubulin beta chain / type: protein_or_peptide / ID: 60 / Number of copies: 142 / Enantiomer: LEVO
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Molecular weight: Theoretical: 49.747926 KDa

Sequence

String:

MREIVCVQAG QCGNQIGSKF WEVISDEHGV DPTGTYQGDS DLQLERINVY FDEATGGRYV PRSVLIDLEP GTMDSVRAGP YGQIFRPDN FIFGQSGAGN NWAKGHYTEG AELIDSVLDV CCKEAESCDC LQGFQICHSL GGGTGSGMGT LLISKLREQY P DRIMMTFS IIPSPKVSDT VVEPYNTTLS VHQLVENSDE SMCIDNEALY DICFRTLKLT TPTFGDLNHL VSAVVSGVTC CL RFPGQLN SDLRKLAVNL VPFPRLHFFM MGFAPLTSRG SQQYRGLSVP ELTQQMFDAK NMMQAADPRH GRYLTASALF RGR MSTKEV DEQMLNVQNK NSSYFIEWIP NNIKSSVCDI PPKGLKMAVT FIGNNTCIQE MFRRVGEQFT LMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATIEEEGE FDEEEQY

UniProtKB: Tubulin beta chain

Macromolecule #61: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 61 / Number of copies: 24 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #62: GUANOSINE-5'-TRIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 62 / Number of copies: 145 / Formula: GTP
• Molecular weight: Theoretical: 523.18 Da

Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

Macromolecule #63: MAGNESIUM ION

• Macromolecule: Name: MAGNESIUM ION / type: ligand / ID: 63 / Number of copies: 145 / Formula: MG
• Molecular weight: Theoretical: 24.305 Da

Macromolecule #64: GUANOSINE-5'-DIPHOSPHATE

• Macromolecule: Name: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 64 / Number of copies: 142 / Formula: GDP
• Molecular weight: Theoretical: 443.201 Da

Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Buffer: pH: 7.4
• Grid: Model: Quantifoil R2/1 / Material: COPPER / Mesh: 200 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

Electron microscopy

• Microscope: TFS KRIOS
• Specialist optics: Energy filter - Name: GIF Quantum LS / Energy filter - Slit width: 20 eV
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Number grids imaged: 2 / Number real images: 106694 / Average exposure time: 2.0 sec. / Average electron dose: 45.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.4 µm / Nominal defocus min: 1.6 µm / Nominal magnification: 81000
• Sample stage: Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• Particle selection: Number selected: 7830948

Startup model

Type of model: EMDB MAP
EMDB ID:

24664

• Final reconstruction: Number classes used: 1 / Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 2.8 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 455012
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD
• Final 3D classification: Number classes: 4 / Avg.num./class: 455012

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: AB INITIO MODEL

Output model

PDB-9e2g:
Cryo-EM structure of 48 nm repeat of microtubule doublet from T. brucei flagellum