PDB-9e5c: Cryo-EM structure of 96 nm repeat of microtubule doublet from T. ...

Basic information

• Entry: Database: PDB / ID: 9e5c
• Title: Cryo-EM structure of 96 nm repeat of microtubule doublet from T. brucei flagellum

Components

• (Actin, putative) x 2
• (Centrin, putative) x 2
• (Cilia- and flagella-associated protein ...) x 14
• (Coiled-coil domain-containing protein ...) x 2
• (DUF4200 domain-containing ...) x 2
• (Dynein arm light chain, axonemal, ...) x 3
• (Dynein heavy chain, ...) x 8
• (Dynein light ...) x 7
• (Dynein regulatory complex subunit ...) x 2
• (EF-hand domain-containing ...) x 9
• (Enkurin domain-containing ...) x 4
• (Flagellar radial spoke ...) x 2
• (Leucine-rich repeat protein ...) x 4
• (Nucleoside diphosphate kinase, ...) x 2
• (Radial spoke protein ...) x 2
• (T. brucei spp.-specific ...) x 2
• (Tetratricopeptide repeat ...) x 2
• (Trichohyalin-plectin-homology domain-containing ...) x 2
• 33 kDa inner dynein arm light chain, axonemal, putative
• ATPase AAA-type core domain-containing protein
• Actin A
• Actin-like protein, putative
• CCDC113/CCDC96 coiled-coil domain-containing protein
• CCDC81 HU domain-containing protein
• CMF34/CARP4
• CMF5
• CMF6
• Calcium-binding protein, putative
• Calmodulin
• Calpain-like cysteine peptidase, putative
• Chaperone protein DNAJ, putative
• Cyclic nucleotide-binding domain-containing protein
• Cyclophilin type peptidyl-prolyl cis-trans isomerase, putative
• Cytochrome b5 domain-containing protein 1
• DRC1
• DRC9
• Dynein axonemal intermediate chain 4
• Dynein intermediate chain, putative
• Dynein regulatory complex protein 10
• Dynein, putative
• FAP107/MC11
• FAP120
• FAP129
• FAP141
• FAP21
• FAP90
• FAP95/MC6
• FAP96B
• FAP96C/MC15
• Flagellar associated protein
• Flagellar protofilament ribbon protein, putative
• IC140
• IC97/Casc1 N-terminal domain-containing protein
• IDAf-alpha
• IQ and ubiquitin-like domain-containing protein
• KIAA1430 homologue
• LIM zinc-binding domain-containing protein
• Leucine-rich repeat-containing protein 51
• MC3
• MC4
• MC5
• MC7
• MC8
• MOP23A
• MOP23B
• MOP23C
• MOP84A
• MORN repeat-containing protein 3
• Meiosis-specific nuclear structural protein 1
• Nucleoside diphosphate kinase
• ODAD1 central coiled coil region domain-containing protein
• Outer dynein arm docking complex
• PACRGA
• PACRGB
• PBP36
• PON3
• PON4
• Peptidyl-prolyl cis-trans isomerase
• Phosphatidylinositol 4-phosphate 5-kinase
• Protein TAX-1
• RSP1
• RSP14
• RSP2
• RSP8
• Radial spoke head protein 9 homolog
• Rib72 protein-like protein
• STOP axonemal protein
• Sperm-tail PG-rich repeat
• Starmaker
• TbDC13
• TbDC65
• TbMIP23
• TbRSP62
• TbRSP63
• TbRib26b
• Transglutaminase-like domain-containing protein
• Trypanin
• Trypanin-related protein, putative
• Tubulin alpha chain
• Tubulin beta chain
• VWFA domain-containing protein

• Keywords: MOTOR PROTEIN / flagella / microtubule

Function / homology

Function:

forward locomotion / regulation of cilium beat frequency / intraciliary transport particle / : / regulation of cilium movement / inner dynein arm / axonemal central apparatus assembly / radial spoke / axonemal dynein complex assembly / outer dynein arm / inner dynein arm assembly / axonemal dynein complex / cilium-dependent cell motility / microtubule anchoring / outer dynein arm assembly / regulation of cilium beat frequency involved in ciliary motility / cilium movement involved in cell motility / 9+2 motile cilium / dynein light chain binding / cilium movement / ciliary transition zone / dynein heavy chain binding / motile cilium assembly / axoneme assembly / cilium organization / axonemal microtubule / cell tip / ciliary tip / dynein axonemal particle / dynein complex / negative regulation of microtubule depolymerization / cell projection organization / nucleoside-diphosphate kinase / minus-end-directed microtubule motor activity / dynein light intermediate chain binding / cytoplasmic dynein complex / Set1C/COMPASS complex / UTP biosynthetic process / ciliary plasm / CTP biosynthetic process / dynein intermediate chain binding / nuclear lumen / non-motile cilium / microtubule associated complex / microtubule motor activity / U3 snoRNA binding / motile cilium / nucleoside diphosphate kinase activity / ciliary rootlet / GTP biosynthetic process / microtubule-based movement / snoRNA binding / positive regulation of cell motility / post-transcriptional regulation of gene expression / cyclosporin A binding / cortical cytoskeleton / phosphate ion binding / beta-tubulin binding / cytoskeletal motor activity / mitotic cytokinesis / : / axoneme / cilium assembly / alpha-tubulin binding / cytoplasmic microtubule / microtubule-based process / enzyme regulator activity / centriole / Hsp70 protein binding / GTPase activator activity / mitotic spindle organization / acrosomal vesicle / ciliary basal body / peptidyl-prolyl cis-trans isomerase activity / meiotic cell cycle / RNA polymerase II CTD heptapeptide repeat P3 isomerase activity / RNA polymerase II CTD heptapeptide repeat P6 isomerase activity / peptidylprolyl isomerase / small-subunit processome / cell motility / Hsp90 protein binding / protein destabilization / structural constituent of cytoskeleton / cilium / small GTPase binding / mitotic spindle / microtubule cytoskeleton organization / actin cytoskeleton / unfolded protein binding / mitotic cell cycle / protein folding / protein-folding chaperone binding / microtubule binding / spermatogenesis / microtubule / oxidoreductase activity / cytoskeleton / calmodulin binding / hydrolase activity / ribosome

Domain/homology:

Cilia- and flagella-associated protein 206 / : / Domain of unknown function / Cilia- and flagella-associated protein 61, N-terminal domain / Cilia- and flagella-associated protein 97 / Cilia- and flagella-associated protein 61 / Uncharacterized 22kDa protein in aldolase locus / Cilia- and flagella-associated protein 61, N-terminal domain / Dynein-f, intermediate chain IC97/Dnai7-like / Domain of unknown function DUF4200 / IC97/Casc1, N-terminal / : / : / : / : / : / Domain of unknown function (DUF4200) / Cancer susceptibility candidate 1 N-terminus / CFAP58 central coiled coil / Tubby, N-terminal / Protein of unknown function DUF4464 / Domain of unknown function (DUF4464) / Axonemal dynein light chain / Dynein regulatory complex protein 1, C-terminal / : / : / Axonemal dynein light chain / Sperm tail C-terminal domain / Hemingway/CFA97 / Dynein regulatory complex subunit 7 / Dynein regulatory complex protein 10 / Radial spoke protein 14 / : / : / : / Hemingway/CFA97 / Domain of unknown function DUF4201 / Cilia- and flagella-associated protein 91 / CFAP91 domain / Coiled-coil domain-containing protein 39 / Coiled-coil domain-containing protein 40 / Dynein regulatory complex protein 9 / : / : / : / CCDC113/CCDC96, coiled-coil / Cilia- and flagella-associated protein 91 / IQ and ubiquitin-like domain-containing protein / Growth arrest-specific protein 8 domain / CCDC81, HU domain 1 / Protein of unknown function DUF4586 / Outer dynein arm-docking complex subunit 3 / Growth arrest-specific protein 8 / Dynein regulatory complex protein 1/2, N-terminal / Dynein regulatory complex protein / CCDC81, HU domain 2 / von Willebrand factor type A domain / Growth-arrest specific micro-tubule binding / Sperm tail / CCDC81 eukaryotic HU domain 1 / Cilia-and flagella-associated protein 96 / CCDC81 eukaryotic HU domain 2 / : / : / Radial spokehead-like protein / Radial spoke 3 / Sperm-tail PG-rich repeat / Radial spokehead-like protein / Radial spoke protein 3 / Sperm-tail PG-rich repeat / : / : / : / ODAD1 central coiled coil region / BRE1 E3 ubiquitin ligase / Sdc1/DPY30 / : / Dpy-30 motif / : / Dpy-30 motif / : / Meiosis-specific nuclear structural protein 1 / Cilia- and flagella-associated protein 45 / NDPK7, second NDPk domain / Cilia- and flagella- associated protein 210 / CFAP53/TCHP / Trichohyalin-plectin-homology domain / Trichohyalin-plectin-homology domain / : / RIB43A / RIB43A / DM10 domain / EF-hand domain-containing protein EFHC1/EFHC2/EFHB / DM10 domain / DM10 domain profile. / Domains in hypothetical proteins in Drosophila, C. elegans and mammals. Occurs singly in some nucleoside diphosphate kinases. / Dynein light chain Tctex-1 like / Dynein light chain roadblock-type 1/2 / Dynein heavy chain 3, AAA+ lid domain / Enkurin domain

Component:

ADENOSINE-5'-DIPHOSPHATE / ADENOSINE-5'-TRIPHOSPHATE / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Peptidyl-prolyl cis-trans isomerase / Calpain-like cysteine peptidase, putative / DUF4200 domain-containing protein / Chaperone protein DNAJ, putative / Dynein, putative / Centrin, putative / Calmodulin / Cilia- and flagella-associated protein 299 / Dynein heavy chain, putative / Trichohyalin-plectin-homology domain-containing protein / Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein / CCDC81 HU domain-containing protein / Uncharacterized protein / Uncharacterized protein / Actin-like protein, putative / Uncharacterized protein / EF-hand domain-containing protein / Uncharacterized protein / Dynein heavy chain, putative / T. brucei spp.-specific protein / Cilia- and flagella-associated protein 52 / Uncharacterized protein / Dynein regulatory complex subunit 2 / Flagellar associated protein / EF-hand domain-containing protein / Leucine-rich repeat protein (LRRP) / Uncharacterized protein / Dynein arm light chain, axonemal, putative / Dynein intermediate chain, putative / Uncharacterized protein / Enkurin domain-containing protein / DUF4200 domain-containing protein / Flagellar radial spoke component, putative / Uncharacterized protein / Actin, putative / Nucleoside diphosphate kinase / Trichohyalin-plectin-homology domain-containing protein / Dynein light chain, putative / ATPase AAA-type core domain-containing protein / Dynein heavy chain, putative / Cilia- and flagella-associated protein 91 / Uncharacterized protein / Radial spoke head protein 9 homolog / Uncharacterized protein / DM10 domain-containing protein / Radial spoke protein 3, putative / Cyclic nucleotide-binding domain-containing protein / EF-hand domain-containing protein / MORN repeat-containing protein 3 / Uncharacterized protein / Uncharacterized protein / Dynein light chain roadblock / 33 kDa inner dynein arm light chain, axonemal, putative / Coiled-coil domain-containing protein 40 / Uncharacterized protein / Centrin, putative / Uncharacterized protein / Rib72 protein-like protein / Uncharacterized protein / EF-hand domain-containing protein / Dynein light chain / KIAA1430 homologue / Trypanin / Dynein heavy chain, putative / Dynein light chain / Uncharacterized protein / CFA20 domain-containing protein / Cytochrome b5 domain-containing protein 1 / Trypanin-related protein, putative / Dynein light chain roadblock / Dynein light chain, putative / Nucleoside diphosphate kinase, putative / Sperm-tail PG-rich repeat / Protein TAX-1 / Uncharacterized protein / Actin A / T. brucei spp.-specific protein / Actin, putative / EF-hand domain-containing protein / Cyclophilin type peptidyl-prolyl cis-trans isomerase, putative / Uncharacterized protein / Dynein light chain / Dynein arm light chain, axonemal, putative / Leucine-rich repeat protein (LRRP) / Tubulin alpha chain / Tubulin beta chain / Enkurin domain-containing protein / Dynein regulatory complex protein 10 / Uncharacterized protein / Cilia- and flagella-associated protein 263 / Uncharacterized protein / Uncharacterized protein / Flagellar protofilament ribbon protein, putative / Cilia- and flagella-associated protein 45 / Uncharacterized protein / Cilia- and flagella-associated protein 53

• Biological species: Trypanosoma brucei brucei TREU927 (eukaryote)
• Method: ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å
• Authors: Xia, X. / Shimogawa, M.M. / Wang, H. / Liu, S. / Wijono, A. / Langousis, G. / Kassem, A.M. / Wohlschlegel, J.A. / Hill, K. / Zhou, Z.H.

Funding support

United States, 2items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	R01GM071940	United States
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	R01AI052348	United States

• Citation: Journal: Science / Year: 2025; Title: Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms.; Authors: Xian Xia / Michelle M Shimogawa / Hui Wang / Samuel Liu / Angeline Wijono / Gerasimos Langousis / Ahmad M Kassem / James A Wohlschlegel / Kent L Hill / Z Hong Zhou / ; Abstract: The flagellum of drives the parasite's characteristic screw-like motion and is essential for its replication, transmission, and pathogenesis. However, the molecular details of this process remain unclear. Here, we present high-resolution (up to 2.8 angstrom) cryo-electron microscopy structures of flagellar doublet microtubules (DMTs). Integrated modeling identified 154 different axonemal proteins inside and outside the DMT and, together with genetic and proteomic interrogation, revealed conserved and trypanosome-specific foundations of flagellum assembly and motility. We captured axonemal dynein motors in their pre-power stroke state. Comparing atomic models between pre- and post-power strokes defined how dynein structural changes drive sliding of adjacent DMTs during flagellar beating. This study illuminates structural dynamics underlying flagellar motility and identifies pathogen-specific proteins to consider for therapeutic interventions targeting neglected diseases.

History

Deposition	Oct 28, 2024	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Mar 12, 2025	Provider: repository / Type: Initial release
Revision 1.1	Mar 26, 2025	Group: Data collection / Database references / Category: citation / citation_author / em_admin Item: _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name / _em_admin.last_update

Assembly

Deposited unit

0A: EF-hand domain-containing family member C2

0B: EF-hand domain-containing family member C2

0C: EF-hand domain-containing family member C2

0D: EF-hand domain-containing family member C2

0E: EF-hand domain-containing family member C2

0F: EF-hand domain-containing family member C2

0G: EF-hand domain-containing family member C2

0H: EF-hand domain-containing family member C2

0I: EF-hand domain-containing family member C2

0J: Rib72 protein-like protein

0K: Rib72 protein-like protein

0L: Rib72 protein-like protein

0M: CMF34/CARP4

0N: CMF34/CARP4

0O: Flagellar protofilament ribbon protein, putative

0P: Flagellar protofilament ribbon protein, putative

0Q: Flagellar protofilament ribbon protein, putative

0R: Flagellar protofilament ribbon protein, putative

0S: Flagellar protofilament ribbon protein, putative

0T: Flagellar protofilament ribbon protein, putative

0U: Cilia- and flagella-associated protein 53

0V: Cilia- and flagella-associated protein 53

0W: Cilia- and flagella-associated protein 53

0X: Cilia- and flagella-associated protein 53

0Y: Meiosis-specific nuclear structural protein 1

0Z: Meiosis-specific nuclear structural protein 1

0a: KIAA1430 homologue

0b: KIAA1430 homologue

0c: KIAA1430 homologue

0d: KIAA1430 homologue

0e: KIAA1430 homologue

0f: KIAA1430 homologue

0g: Starmaker

0h: Starmaker

0i: Starmaker

0j: Starmaker

0k: Starmaker

0l: Starmaker

0m: Starmaker

0n: Starmaker

0o: Starmaker

0p: Starmaker

0q: LIM zinc-binding domain-containing protein

0r: Sperm-tail PG-rich repeat

0s: Sperm-tail PG-rich repeat

0t: MOP84A

0u: Leucine-rich repeat protein (LRRP)

0v: Coiled-coil domain-containing protein 39

0w: Coiled-coil domain-containing protein 39

0x: Coiled-coil domain-containing protein 40

0y: Coiled-coil domain-containing protein 40

0z: Cilia- and flagella-associated protein 299

1A: Meiosis-specific nuclear structural protein 1

1B: Meiosis-specific nuclear structural protein 1

1C: Nucleoside diphosphate kinase, putative

1D: Nucleoside diphosphate kinase, putative

1E: Nucleoside diphosphate kinase, putative

1F: Nucleoside diphosphate kinase, putative

1G: Nucleoside diphosphate kinase, putative

1H: EF-hand domain-containing protein

1I: EF-hand domain-containing protein

1J: EF-hand domain-containing protein

1K: EF-hand domain-containing protein

1L: Cyclic nucleotide-binding domain-containing protein

1M: Cyclic nucleotide-binding domain-containing protein

1N: TbMIP23

1O: TbMIP23

1P: FAP141

1Q: FAP141

1R: EF-hand domain-containing protein

1S: EF-hand domain-containing protein

1T: Calpain-like cysteine peptidase, putative

1U: Calpain-like cysteine peptidase, putative

1V: Calpain-like cysteine peptidase, putative

1W: EF-hand domain-containing protein

1X: EF-hand domain-containing protein

1Y: Calcium-binding protein, putative

1Z: Calcium-binding protein, putative

1a: Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein

1b: Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein

1c: CMF5

1d: CMF6

1e: CCDC113/CCDC96 coiled-coil domain-containing protein

1f: Cilia- and flagella-associated protein 263

1g: DRC1

1h: Dynein regulatory complex subunit 2

1i: Dynein regulatory complex subunit 3

1j: Trypanin-related protein, putative

1k: Trypanin

1l: Leucine-rich repeat protein (LRRP)

1m: Transglutaminase-like domain-containing protein

1n: DRC9

1o: Dynein regulatory complex protein 10

1p: ATPase AAA-type core domain-containing protein

1r: Cilia- and flagella-associated protein 43

1t: Cilia- and flagella-associated protein 44

2A: EF-hand domain-containing protein

2B: EF-hand domain-containing protein

2C: EF-hand domain-containing protein

2D: Peptidyl-prolyl cis-trans isomerase

2E: Peptidyl-prolyl cis-trans isomerase

2F: Peptidyl-prolyl cis-trans isomerase

2G: EF-hand domain-containing protein

2H: EF-hand domain-containing protein

2I: EF-hand domain-containing protein

2J: FAP107/MC11

2K: FAP107/MC11

2L: FAP107/MC11

2M: T. brucei spp.-specific protein

2N: T. brucei spp.-specific protein

2O: T. brucei spp.-specific protein

2P: FAP95/MC6

2Q: FAP95/MC6

2R: FAP129

2S: FAP129

2T: FAP129

2U: T. brucei spp.-specific protein

2V: T. brucei spp.-specific protein

2W: T. brucei spp.-specific protein

2X: FAP21

2Y: FAP21

2Z: FAP21

2a: RSP1

2b: RSP1

2c: RSP1

2d: RSP1

2e: RSP2

2f: RSP2

2g: RSP2

2h: RSP2

2i: Radial spoke protein 3, putative

2j: Radial spoke protein 3, putative

2k: Radial spoke protein 3, putative

2l: Radial spoke protein 3, putative

2m: Flagellar radial spoke component, putative

2n: Flagellar radial spoke component, putative

2o: Flagellar radial spoke component, putative

2p: Flagellar radial spoke component, putative

2q: Flagellar radial spoke protein-like, putative

2r: Flagellar radial spoke protein-like, putative

2s: Flagellar radial spoke protein-like, putative

2t: Flagellar radial spoke protein-like, putative

2u: RSP8

2v: Radial spoke head protein 9 homolog

2w: Radial spoke head protein 9 homolog

2x: Radial spoke head protein 9 homolog

2y: Radial spoke head protein 9 homolog

2z: Radial spoke protein RSP9

3A: Flagellar associated protein

3B: Flagellar associated protein

3C: Flagellar associated protein

3D: FAP21

3E: TbRib26b

3F: TbRib26b

3G: TbRib26b

3H: TbRib26b

3I: TbRib26b

3J: EF-hand domain-containing protein

3K: EF-hand domain-containing protein

3L: EF-hand domain-containing protein

3M: PACRGA

3N: PACRGA

3O: PACRGA

3P: PACRGA

3Q: PACRGA

3R: PACRGA

3S: PACRGA

3T: PACRGA

3U: PACRGB

3V: PACRGB

3W: PACRGB

3X: PACRGB

3Y: PACRGB

3Z: Cilia- and flagella-associated protein 20

3a: Radial spoke protein RSP9

3b: Radial spoke protein RSP9

3c: Radial spoke protein RSP9

3d: Phosphatidylinositol 4-phosphate 5-kinase

3e: Phosphatidylinositol 4-phosphate 5-kinase

3f: Phosphatidylinositol 4-phosphate 5-kinase

3g: Phosphatidylinositol 4-phosphate 5-kinase

3h: Cyclophilin type peptidyl-prolyl cis-trans isomerase, putative

3i: Cyclophilin type peptidyl-prolyl cis-trans isomerase, putative

3j: RSP14

3k: Leucine-rich repeat protein (LRRP)

3l: Chaperone protein DNAJ, putative

3m: Chaperone protein DNAJ, putative

3o: Chaperone protein DNAJ, putative

3p: Chaperone protein DNAJ, putative

3q: Nucleoside diphosphate kinase

3r: Nucleoside diphosphate kinase

3s: Nucleoside diphosphate kinase

3t: Nucleoside diphosphate kinase

3u: Dynein light chain

3v: Dynein light chain

3w: Dynein light chain

3x: Dynein light chain

3y: Dynein light chain

3z: Dynein light chain

4A: Cilia- and flagella-associated protein 20

4B: Cilia- and flagella-associated protein 20

4C: Cilia- and flagella-associated protein 20

4D: Cilia- and flagella-associated protein 20

4E: Cilia- and flagella-associated protein 20

4F: Cilia- and flagella-associated protein 20

4G: Cilia- and flagella-associated protein 20

4H: Cilia- and flagella-associated protein 20

4I: Cilia- and flagella-associated protein 20

4J: Cilia- and flagella-associated protein 20

4K: Cilia- and flagella-associated protein 20

4L: Cilia- and flagella-associated protein 20

4M: Cilia- and flagella-associated protein 20

4N: Cilia- and flagella-associated protein 20

4O: Cilia- and flagella-associated protein 52

4P: Cilia- and flagella-associated protein 52

4Q: Cilia- and flagella-associated protein 52

4R: Cilia- and flagella-associated protein 52

4S: Cilia- and flagella-associated protein 52

4T: Cilia- and flagella-associated protein 52

4U: Cilia- and flagella-associated protein 52

4V: Enkurin domain-containing protein

4W: Enkurin domain-containing protein

4X: Enkurin domain-containing protein

4Y: Enkurin domain-containing protein

4Z: Enkurin domain-containing protein

4a: Dynein light chain

4b: Dynein light chain

4c: Dynein light chain

4d: Dynein light chain

4e: Dynein light chain

4f: Dynein light chain

4g: Dynein light chain

4h: Dynein light chain

4i: Dynein light chain

4j: Dynein light chain

4k: Dynein light chain

4l: Dynein light chain

4m: Dynein light chain

4n: Dynein light chain

4o: Cytochrome b5 domain-containing protein 1

4p: Cytochrome b5 domain-containing protein 1

4q: Leucine-rich repeat protein (LRRP)

4r: Leucine-rich repeat protein (LRRP)

4s: VWFA domain-containing protein

4t: VWFA domain-containing protein

4u: TbRSP63

4v: TbRSP63

4w: IQ and ubiquitin-like domain-containing protein

4x: Calmodulin

4y: MORN repeat-containing protein 3

4z: Cilia- and flagella-associated protein 206

5A: Enkurin domain-containing protein

5B: Enkurin domain-containing protein

5C: MC4

5D: MC4

5E: MC4

5F: MC4

5G: MC4

5H: MC4

5I: MC4

5J: MC4

5K: Cilia- and flagella-associated protein 45

5L: CCDC81 HU domain-containing protein

5M: CCDC81 HU domain-containing protein

5N: MC5

5O: MC5

5P: MC5

5Q: MC5

5R: MC5

5S: Cilia- and flagella-associated protein 45

5T: Cilia- and flagella-associated protein 45

5U: Cilia- and flagella-associated protein 45

5V: Cilia- and flagella-associated protein 45

5W: Cilia- and flagella-associated protein 45

5X: Cilia- and flagella-associated protein 45

5Y: Trichohyalin-plectin-homology domain-containing protein

5Z: Trichohyalin-plectin-homology domain-containing protein

5a: TbRSP62

5b: Cilia- and flagella-associated protein 91

5c: Cilia- and flagella-associated protein 251

5d: Cilia- and flagella-associated protein 61 N-terminal domain-containing protein

5e: DUF4200 domain-containing protein

5f: DUF4200 domain-containing protein

5g: Leucine-rich repeat-containing protein 51

5h: IDAf-alpha

5i: IDAf-alpha

5j: Dynein heavy chain, putative

5k: Dynein heavy chain, putative

5l: Dynein axonemal intermediate chain 4

5m: IC140

5n: IC97/Casc1 N-terminal domain-containing protein

5o: FAP120

5p: Dynein light chain, putative

5q: Dynein light chain roadblock

5r: Dynein light chain roadblock

5s: Dynein light chain

5t: Dynein light chain

5u: Dynein light chain

5v: Dynein light chain

5w: Dynein light chain

5x: Dynein light chain

5y: Dynein light chain, putative

6A: Trichohyalin-plectin-homology domain-containing protein

6B: Trichohyalin-plectin-homology domain-containing protein

6C: Trichohyalin-plectin-homology domain-containing protein

6D: Trichohyalin-plectin-homology domain-containing protein

6E: Trichohyalin-plectin-homology domain-containing protein

6F: Trichohyalin-plectin-homology domain-containing protein

6G: Trichohyalin-plectin-homology domain-containing protein

6H: Trichohyalin-plectin-homology domain-containing protein

6I: Trichohyalin-plectin-homology domain-containing protein

6J: Trichohyalin-plectin-homology domain-containing protein

6K: Trichohyalin-plectin-homology domain-containing protein

6L: Trichohyalin-plectin-homology domain-containing protein

6M: Trichohyalin-plectin-homology domain-containing protein

6N: Trichohyalin-plectin-homology domain-containing protein

6O: Trichohyalin-plectin-homology domain-containing protein

6P: Trichohyalin-plectin-homology domain-containing protein

6Q: Trichohyalin-plectin-homology domain-containing protein

6R: STOP axonemal protein

6S: STOP axonemal protein

6T: STOP axonemal protein

6U: STOP axonemal protein

6V: STOP axonemal protein

6W: STOP axonemal protein

6X: STOP axonemal protein

6Y: STOP axonemal protein

6Z: STOP axonemal protein

6a: Dynein heavy chain, putative

6b: Dynein heavy chain, putative

6c: Dynein heavy chain, putative

6d: Dynein heavy chain, putative

6e: Dynein heavy chain, putative

6f: Actin A

6g: Actin A

6h: Actin-like protein, putative

6i: Actin, putative

6j: Actin, putative

6k: Dynein arm light chain, axonemal, putative

6l: Dynein arm light chain, axonemal, putative

6m: Dynein arm light chain, axonemal, putative

6n: 33 kDa inner dynein arm light chain, axonemal, putative

6o: Dynein arm light chain, axonemal, putative

6p: Dynein arm light chain, axonemal, putative

6q: Protein TAX-1

6r: Tetratricopeptide repeat protein 29

6s: Tetratricopeptide repeat protein

6t: Centrin, putative

6u: Centrin, putative

7A: STOP axonemal protein

7B: STOP axonemal protein

7C: STOP axonemal protein

7D: STOP axonemal protein

7E: STOP axonemal protein

7F: STOP axonemal protein

7G: STOP axonemal protein

7H: STOP axonemal protein

7I: STOP axonemal protein

7J: STOP axonemal protein

7K: STOP axonemal protein

7L: MC8

7M: MC8

7N: MC3

7O: MC3

7P: MC3

7Q: FAP90

7R: FAP90

7S: FAP90

7T: PBP36

7U: PBP36

7V: Enkurin domain-containing protein

7W: Enkurin domain-containing protein

7X: Enkurin domain-containing protein

7Y: Enkurin domain-containing protein

7Z: Enkurin domain-containing protein

7a: Dynein heavy chain, putative

7b: Dynein heavy chain, putative

7c: TbDC65

7d: TbDC13

7e: Dynein heavy chain, putative

7f: Dynein heavy chain, putative

7g: Dynein, putative

7h: Dynein intermediate chain, putative

7i: Dynein light chain, putative

7j: Dynein light chain

7k: Dynein light chain

7l: Dynein light chain roadblock

7m: Dynein light chain roadblock

7n: Dynein light chain

7o: Dynein light chain

7p: Dynein light chain

7q: Dynein light chain, putative

7r: Dynein light chain

7s: Outer dynein arm docking complex

7t: ODAD1 central coiled coil region domain-containing protein

8A: Enkurin domain-containing protein

8B: Enkurin domain-containing protein

8C: Enkurin domain-containing protein

8D: Enkurin domain-containing protein

8E: Enkurin domain-containing protein

8F: Enkurin domain-containing protein

8G: Enkurin domain-containing protein

8H: Enkurin domain-containing protein

8I: Enkurin domain-containing protein

8J: Enkurin domain-containing protein

8K: Enkurin domain-containing protein

8L: PBP36

8M: PON3

8N: PON3

8O: PON3

8P: PON3

8Q: PON3

8R: PON3

8S: PON3

8T: PON3

8U: PON4

8V: PON4

8W: PON4

8X: PON4

8Y: PON4

8Z: PON4

8c: TbDC65

8d: TbDC13

8e: Dynein heavy chain, putative

8f: Dynein heavy chain, putative

8g: Dynein, putative

8h: Dynein intermediate chain, putative

8i: Dynein light chain, putative

8j: Dynein light chain

8k: Dynein light chain

8l: Dynein light chain roadblock

8m: Dynein light chain roadblock

8n: Dynein light chain

8o: Dynein light chain

8p: Dynein light chain

8q: Dynein light chain, putative

8r: Dynein light chain

8s: Outer dynein arm docking complex

8t: ODAD1 central coiled coil region domain-containing protein

9A: PON4

9B: PON4

9C: MC7

9D: MC7

9E: MC7

9F: FAP96C/MC15

9G: FAP96C/MC15

9H: FAP96C/MC15

9I: FAP96C/MC15

9J: FAP96B

9K: FAP96B

9L: FAP96B

9M: MOP23A

9N: MOP23A

9O: MOP23A

9P: MOP23A

9Q: MOP23A

9R: MOP23A

9S: MOP23A

9T: MOP23B

9U: MOP23B

9V: MOP23B

9W: MOP23C

9X: MOP23C

9Y: MOP23C

9Z: STOP axonemal protein

9a: Outer dynein arm docking complex

9b: ODAD1 central coiled coil region domain-containing protein

9c: TbDC65

9d: TbDC13

9e: Dynein heavy chain, putative

9f: Dynein heavy chain, putative

9g: Dynein, putative

9h: Dynein intermediate chain, putative

9i: Dynein light chain, putative

9j: Dynein light chain

9k: Dynein light chain

9l: Dynein light chain roadblock

9m: Dynein light chain roadblock

9n: Dynein light chain

9o: Dynein light chain

9p: Dynein light chain

9q: Dynein light chain, putative

9r: Dynein light chain

9s: Outer dynein arm docking complex

9t: ODAD1 central coiled coil region domain-containing protein

AA: Tubulin beta chain

AB: Tubulin alpha chain

AC: Tubulin beta chain

AD: Tubulin alpha chain

AE: Tubulin beta chain

AF: Tubulin alpha chain

AG: Tubulin beta chain

AH: Tubulin alpha chain

AI: Tubulin beta chain

AJ: Tubulin alpha chain

AK: Tubulin beta chain

AL: Tubulin alpha chain

AM: Tubulin beta chain

AN: Tubulin alpha chain

AO: Tubulin beta chain

AP: Tubulin alpha chain

AQ: Tubulin beta chain

AR: Tubulin alpha chain

AS: Tubulin beta chain

AT: Tubulin alpha chain

AU: Tubulin beta chain

AV: Tubulin alpha chain

AW: Tubulin beta chain

AX: Tubulin alpha chain

AY: Tubulin beta chain

AZ: Tubulin alpha chain

Aa: Tubulin beta chain

Ab: Tubulin alpha chain

Ac: Tubulin beta chain

Ad: Tubulin alpha chain

Ae: Tubulin beta chain

Af: Tubulin alpha chain

Ag: Tubulin beta chain

Ah: Tubulin alpha chain

Ai: Tubulin beta chain

Aj: Tubulin alpha chain

Ak: Tubulin beta chain

Al: Tubulin alpha chain

Am: Tubulin beta chain

An: Tubulin alpha chain

Ao: Tubulin beta chain

Ap: Tubulin alpha chain

BA: Tubulin beta chain

BB: Tubulin alpha chain

BC: Tubulin beta chain

BD: Tubulin alpha chain

BF: Tubulin alpha chain

BG: Tubulin beta chain

BH: Tubulin alpha chain

BI: Tubulin beta chain

BJ: Tubulin alpha chain

BK: Tubulin beta chain

BL: Tubulin alpha chain

BM: Tubulin beta chain

BN: Tubulin alpha chain

BO: Tubulin beta chain

BP: Tubulin alpha chain

BQ: Tubulin beta chain

BR: Tubulin alpha chain

BS: Tubulin beta chain

BT: Tubulin alpha chain

BU: Tubulin beta chain

BV: Tubulin alpha chain

BW: Tubulin beta chain

BX: Tubulin alpha chain

BY: Tubulin beta chain

BZ: Tubulin alpha chain

CA: Tubulin beta chain

CB: Tubulin alpha chain

CC: Tubulin beta chain

CD: Tubulin alpha chain

CE: Tubulin beta chain

CF: Tubulin alpha chain

CH: Tubulin beta chain

CI: Tubulin alpha chain

CJ: Tubulin alpha chain

CK: Tubulin beta chain

CL: Tubulin alpha chain

CM: Tubulin beta chain

CN: Tubulin alpha chain

CO: Tubulin beta chain

CP: Tubulin alpha chain

CQ: Tubulin beta chain

CR: Tubulin alpha chain

CS: Tubulin beta chain

CT: Tubulin alpha chain

CU: Tubulin beta chain

CV: Tubulin alpha chain

CW: Tubulin beta chain

CX: Tubulin alpha chain

CY: Tubulin beta chain

CZ: Tubulin alpha chain

DA: Tubulin beta chain

DB: Tubulin alpha chain

DC: Tubulin beta chain

DD: Tubulin alpha chain

DE: Tubulin beta chain

DF: Tubulin alpha chain

DG: Tubulin beta chain

DH: Tubulin alpha chain

DI: Tubulin beta chain

DJ: Tubulin alpha chain

DK: Tubulin beta chain

DL: Tubulin alpha chain

DM: Tubulin alpha chain

DN: Tubulin beta chain

DO: Tubulin alpha chain

DP: Tubulin beta chain

DQ: Tubulin alpha chain

DR: Tubulin beta chain

DS: Tubulin alpha chain

DT: Tubulin beta chain

DU: Tubulin alpha chain

DV: Tubulin beta chain

DW: Tubulin alpha chain

DX: Tubulin beta chain

DY: Tubulin alpha chain

DZ: Tubulin beta chain

EA: Tubulin alpha chain

EB: Tubulin beta chain

EC: Tubulin alpha chain

ED: Tubulin beta chain

EE: Tubulin alpha chain

EF: Tubulin beta chain

EG: Tubulin alpha chain

EH: Tubulin beta chain

EI: Tubulin alpha chain

EJ: Tubulin beta chain

EK: Tubulin alpha chain

EL: Tubulin beta chain

EM: Tubulin alpha chain

EN: Tubulin beta chain

EO: Tubulin alpha chain

EQ: Tubulin alpha chain

ER: Tubulin beta chain

ES: Tubulin alpha chain

ET: Tubulin beta chain

EU: Tubulin alpha chain

EV: Tubulin beta chain

EW: Tubulin alpha chain

EX: Tubulin beta chain

EY: Tubulin alpha chain

EZ: Tubulin beta chain

FA: Tubulin alpha chain

FB: Tubulin beta chain

FC: Tubulin alpha chain

FD: Tubulin beta chain

FE: Tubulin alpha chain

FF: Tubulin beta chain

FG: Tubulin alpha chain

FH: Tubulin beta chain

FI: Tubulin alpha chain

FJ: Tubulin beta chain

FK: Tubulin alpha chain

FL: Tubulin beta chain

FM: Tubulin alpha chain

FN: Tubulin beta chain

FO: Tubulin alpha chain

FP: Tubulin beta chain

FQ: Tubulin alpha chain

FR: Tubulin beta chain

FS: Tubulin alpha chain

FT: Tubulin beta chain

FV: Tubulin beta chain

FW: Tubulin alpha chain

FX: Tubulin beta chain

FY: Tubulin alpha chain

FZ: Tubulin beta chain

GA: Tubulin alpha chain

GB: Tubulin beta chain

GC: Tubulin alpha chain

GD: Tubulin beta chain

GE: Tubulin alpha chain

GF: Tubulin beta chain

GG: Tubulin alpha chain

GH: Tubulin beta chain

GI: Tubulin alpha chain

GJ: Tubulin beta chain

GK: Tubulin alpha chain

GL: Tubulin beta chain

GM: Tubulin alpha chain

GN: Tubulin beta chain

GO: Tubulin alpha chain

GP: Tubulin beta chain

GQ: Tubulin alpha chain

GR: Tubulin beta chain

GS: Tubulin alpha chain

GT: Tubulin beta chain

GU: Tubulin alpha chain

GV: Tubulin beta chain

GW: Tubulin alpha chain

GX: Tubulin beta chain

GZ: Tubulin beta chain

HA: Tubulin alpha chain

HB: Tubulin beta chain

HC: Tubulin alpha chain

HD: Tubulin beta chain

HE: Tubulin alpha chain

HF: Tubulin beta chain

HG: Tubulin alpha chain

HH: Tubulin beta chain

HI: Tubulin alpha chain

HJ: Tubulin beta chain

HK: Tubulin alpha chain

HL: Tubulin beta chain

HM: Tubulin alpha chain

HN: Tubulin beta chain

HO: Tubulin alpha chain

HP: Tubulin beta chain

HQ: Tubulin alpha chain

HR: Tubulin beta chain

HS: Tubulin alpha chain

HT: Tubulin beta chain

HU: Tubulin alpha chain

HV: Tubulin beta chain

HW: Tubulin alpha chain

HX: Tubulin beta chain

HY: Tubulin alpha chain

HZ: Tubulin beta chain

IA: Tubulin alpha chain

IB: Tubulin beta chain

ID: Tubulin beta chain

IE: Tubulin alpha chain

IF: Tubulin beta chain

IG: Tubulin alpha chain

IH: Tubulin beta chain

II: Tubulin alpha chain

IJ: Tubulin beta chain

IK: Tubulin alpha chain

IL: Tubulin beta chain

IM: Tubulin alpha chain

IN: Tubulin beta chain

IO: Tubulin alpha chain

IP: Tubulin beta chain

IQ: Tubulin alpha chain

IR: Tubulin beta chain

IS: Tubulin alpha chain

IT: Tubulin beta chain

IU: Tubulin alpha chain

IV: Tubulin beta chain

IW: Tubulin alpha chain

IX: Tubulin beta chain

IY: Tubulin alpha chain

IZ: Tubulin beta chain

JA: Tubulin alpha chain

JB: Tubulin beta chain

JC: Tubulin alpha chain

JD: Tubulin beta chain

JE: Tubulin alpha chain

JF: Tubulin beta chain

JG: Tubulin beta chain

JH: Tubulin alpha chain

JI: Tubulin beta chain

JJ: Tubulin alpha chain

JK: Tubulin beta chain

JL: Tubulin alpha chain

JM: Tubulin beta chain

JN: Tubulin alpha chain

JO: Tubulin beta chain

JP: Tubulin alpha chain

JQ: Tubulin beta chain

JR: Tubulin alpha chain

JS: Tubulin beta chain

JT: Tubulin alpha chain

JU: Tubulin beta chain

JV: Tubulin alpha chain

JW: Tubulin beta chain

JX: Tubulin alpha chain

JY: Tubulin beta chain

JZ: Tubulin alpha chain

KA: Tubulin beta chain

KB: Tubulin alpha chain

KC: Tubulin beta chain

KD: Tubulin alpha chain

KE: Tubulin beta chain

KF: Tubulin alpha chain

KG: Tubulin beta chain

KH: Tubulin alpha chain

KI: Tubulin beta chain

KJ: Tubulin alpha chain

KK: Tubulin beta chain

KL: Tubulin alpha chain

KM: Tubulin beta chain

KN: Tubulin alpha chain

KO: Tubulin beta chain

KP: Tubulin alpha chain

KQ: Tubulin beta chain

KR: Tubulin alpha chain

KS: Tubulin beta chain

KT: Tubulin alpha chain

KU: Tubulin beta chain

KV: Tubulin alpha chain

KW: Tubulin beta chain

KX: Tubulin alpha chain

KY: Tubulin beta chain

KZ: Tubulin alpha chain

LA: Tubulin beta chain

LB: Tubulin alpha chain

LC: Tubulin beta chain

LD: Tubulin alpha chain

LE: Tubulin beta chain

LF: Tubulin alpha chain

LG: Tubulin beta chain

LH: Tubulin alpha chain

LI: Tubulin beta chain

LJ: Tubulin alpha chain

LK: Tubulin beta chain

LL: Tubulin alpha chain

LM: Tubulin beta chain

LN: Tubulin alpha chain

LO: Tubulin beta chain

LP: Tubulin alpha chain

LQ: Tubulin beta chain

LR: Tubulin alpha chain

LS: Tubulin beta chain

LT: Tubulin alpha chain

LU: Tubulin beta chain

LV: Tubulin alpha chain

LW: Tubulin beta chain

LX: Tubulin alpha chain

LY: Tubulin beta chain

LZ: Tubulin alpha chain

MA: Tubulin beta chain

MB: Tubulin alpha chain

MC: Tubulin beta chain

MD: Tubulin alpha chain

ME: Tubulin beta chain

MF: Tubulin alpha chain

MG: Tubulin beta chain

MH: Tubulin alpha chain

MI: Tubulin beta chain

MJ: Tubulin alpha chain

MK: Tubulin beta chain

ML: Tubulin alpha chain

MM: Tubulin beta chain

MN: Tubulin alpha chain

MO: Tubulin beta chain

MP: Tubulin alpha chain

MQ: Tubulin beta chain

MR: Tubulin alpha chain

MT: Tubulin alpha chain

MU: Tubulin beta chain

MV: Tubulin alpha chain

MW: Tubulin beta chain

MX: Tubulin alpha chain

MY: Tubulin beta chain

MZ: Tubulin alpha chain

NA: Tubulin beta chain

NB: Tubulin alpha chain

NC: Tubulin beta chain

ND: Tubulin alpha chain

NE: Tubulin beta chain

NF: Tubulin alpha chain

NG: Tubulin beta chain

NH: Tubulin alpha chain

NI: Tubulin beta chain

NJ: Tubulin alpha chain

NK: Tubulin beta chain

NL: Tubulin alpha chain

NM: Tubulin beta chain

NN: Tubulin alpha chain

NO: Tubulin beta chain

NP: Tubulin alpha chain

NQ: Tubulin beta chain

NR: Tubulin alpha chain

NS: Tubulin beta chain

NT: Tubulin alpha chain

NU: Tubulin beta chain

NV: Tubulin alpha chain

NX: Tubulin alpha chain

NY: Tubulin beta chain

NZ: Tubulin alpha chain

OA: Tubulin beta chain

OB: Tubulin alpha chain

OC: Tubulin beta chain

OD: Tubulin alpha chain

OE: Tubulin beta chain

OF: Tubulin alpha chain

OG: Tubulin beta chain

OH: Tubulin alpha chain

OI: Tubulin beta chain

OJ: Tubulin alpha chain

OK: Tubulin beta chain

OL: Tubulin alpha chain

OM: Tubulin beta chain

ON: Tubulin alpha chain

OO: Tubulin beta chain

OP: Tubulin alpha chain

OQ: Tubulin beta chain

OR: Tubulin alpha chain

OS: Tubulin beta chain

OT: Tubulin alpha chain

OU: Tubulin beta chain

OV: Tubulin alpha chain

OW: Tubulin beta chain

OX: Tubulin alpha chain

OY: Tubulin beta chain

OZ: Tubulin alpha chain

PA: Tubulin beta chain

PC: Tubulin alpha chain

PD: Tubulin beta chain

PE: Tubulin alpha chain

PF: Tubulin beta chain

PG: Tubulin alpha chain

PH: Tubulin beta chain

PI: Tubulin alpha chain

PJ: Tubulin beta chain

PK: Tubulin alpha chain

PL: Tubulin beta chain

PM: Tubulin alpha chain

PN: Tubulin beta chain

PO: Tubulin alpha chain

PP: Tubulin beta chain

PQ: Tubulin alpha chain

PR: Tubulin beta chain

PS: Tubulin alpha chain

PT: Tubulin beta chain

PU: Tubulin alpha chain

PV: Tubulin beta chain

PW: Tubulin alpha chain

PX: Tubulin beta chain

PY: Tubulin alpha chain

PZ: Tubulin beta chain

QA: Tubulin alpha chain

QB: Tubulin beta chain

QC: Tubulin alpha chain

QD: Tubulin beta chain

QE: Tubulin alpha chain

QF: Tubulin beta chain

QG: Tubulin alpha chain

QH: Tubulin beta chain

QI: Tubulin alpha chain

QJ: Tubulin beta chain

QK: Tubulin alpha chain

QL: Tubulin beta chain

QM: Tubulin alpha chain

QN: Tubulin beta chain

QO: Tubulin alpha chain

QP: Tubulin beta chain

QQ: Tubulin alpha chain

QR: Tubulin beta chain

QS: Tubulin alpha chain

QT: Tubulin beta chain

QU: Tubulin alpha chain

QV: Tubulin beta chain

QW: Tubulin alpha chain

QX: Tubulin beta chain

QY: Tubulin alpha chain

QZ: Tubulin beta chain

RA: Tubulin alpha chain

RB: Tubulin beta chain

RC: Tubulin alpha chain

RD: Tubulin beta chain

RE: Tubulin alpha chain

RF: Tubulin beta chain

RG: Tubulin alpha chain

RH: Tubulin beta chain

RI: Tubulin alpha chain

RJ: Tubulin beta chain

RK: Tubulin alpha chain

RL: Tubulin beta chain

RM: Tubulin alpha chain

RN: Tubulin beta chain

RO: Tubulin alpha chain

RP: Tubulin beta chain

RQ: Tubulin alpha chain

RR: Tubulin beta chain

RS: Tubulin alpha chain

RT: Tubulin beta chain

RU: Tubulin alpha chain

RV: Tubulin beta chain

RW: Tubulin alpha chain

RX: Tubulin beta chain

RY: Tubulin alpha chain

RZ: Tubulin beta chain

SA: Tubulin alpha chain

SB: Tubulin beta chain

SC: Tubulin alpha chain

SD: Tubulin beta chain

SE: Tubulin alpha chain

SF: Tubulin beta chain

SG: Tubulin alpha chain

SH: Tubulin beta chain

SI: Tubulin alpha chain

SJ: Tubulin beta chain

SK: Tubulin alpha chain

SL: Tubulin beta chain

SM: Tubulin alpha chain

SN: Tubulin beta chain

SO: Tubulin alpha chain

SP: Tubulin beta chain

SQ: Tubulin alpha chain

SR: Tubulin beta chain

SS: Tubulin alpha chain

ST: Tubulin beta chain

SU: Tubulin alpha chain

SV: Tubulin beta chain

SW: Tubulin alpha chain

SX: Tubulin beta chain

SY: Tubulin alpha chain

SZ: Tubulin beta chain

TA: Tubulin alpha chain

TB: Tubulin beta chain

TC: Tubulin alpha chain

TD: Tubulin beta chain

TE: Tubulin alpha chain

TF: Tubulin beta chain

TG: Tubulin alpha chain

TH: Tubulin beta chain

TI: Tubulin alpha chain

TJ: Tubulin beta chain

TK: Tubulin alpha chain

TL: Tubulin beta chain

TM: Tubulin alpha chain

TN: Tubulin beta chain

TO: Tubulin alpha chain

TP: Tubulin beta chain

TQ: Tubulin alpha chain

TR: Tubulin alpha chain

TS: Tubulin beta chain

TT: Tubulin alpha chain

TU: Tubulin beta chain

TV: Tubulin alpha chain

TW: Tubulin beta chain

TX: Tubulin alpha chain

TY: Tubulin beta chain

TZ: Tubulin alpha chain

UA: Tubulin beta chain

UB: Tubulin alpha chain

UC: Tubulin beta chain

UD: Tubulin alpha chain

UE: Tubulin beta chain

UF: Tubulin alpha chain

UG: Tubulin beta chain

UH: Tubulin alpha chain

UI: Tubulin beta chain

UJ: Tubulin alpha chain

UK: Tubulin beta chain

UL: Tubulin alpha chain

UM: Tubulin beta chain

UN: Tubulin alpha chain

UO: Tubulin beta chain

UP: Tubulin alpha chain

UQ: Tubulin beta chain

UR: Tubulin alpha chain

US: Tubulin beta chain

UT: Tubulin alpha chain

UU: Tubulin beta chain

UW: Tubulin alpha chain

UX: Tubulin beta chain

UY: Tubulin alpha chain

UZ: Tubulin beta chain

VA: Tubulin alpha chain

VB: Tubulin beta chain

VC: Tubulin alpha chain

VD: Tubulin beta chain

VE: Tubulin alpha chain

VF: Tubulin beta chain

VG: Tubulin alpha chain

VH: Tubulin beta chain

VI: Tubulin alpha chain

VJ: Tubulin beta chain

VK: Tubulin alpha chain

VL: Tubulin beta chain

VM: Tubulin alpha chain

VN: Tubulin beta chain

VO: Tubulin alpha chain

VP: Tubulin beta chain

VQ: Tubulin alpha chain

VR: Tubulin beta chain

VS: Tubulin alpha chain

VT: Tubulin beta chain

VU: Tubulin alpha chain

VV: Tubulin beta chain

VW: Tubulin alpha chain

VX: Tubulin beta chain

VY: Tubulin alpha chain

VZ: Tubulin beta chain

WA: Tubulin alpha chain

WB: Tubulin beta chain

WC: Tubulin alpha chain

WD: Tubulin beta chain

WE: Tubulin alpha chain

WF: Tubulin beta chain

WG: Tubulin alpha chain

WH: Tubulin beta chain

WI: Tubulin alpha chain

WJ: Tubulin beta chain

WK: Tubulin alpha chain

WL: Tubulin beta chain

WM: Tubulin alpha chain

WN: Tubulin beta chain

WO: Tubulin alpha chain

WP: Tubulin beta chain

WQ: Tubulin alpha chain

WR: Tubulin beta chain

WS: Tubulin alpha chain

WT: Tubulin beta chain

WU: Tubulin alpha chain

WV: Tubulin beta chain

WW: Tubulin alpha chain

WX: Tubulin beta chain

WY: Tubulin alpha chain

WZ: Tubulin beta chain

XA: Tubulin alpha chain

XB: Tubulin beta chain

XC: Tubulin alpha chain

XD: Tubulin beta chain

XE: Tubulin alpha chain

XF: Tubulin beta chain

XG: Tubulin alpha chain

XH: Tubulin beta chain

XI: Tubulin alpha chain

XJ: Tubulin beta chain

XK: Tubulin alpha chain

XL: Tubulin beta chain

XM: Tubulin alpha chain

XN: Tubulin beta chain

XO: Tubulin alpha chain

XP: Tubulin beta chain

XQ: Tubulin alpha chain

XR: Tubulin beta chain

XS: Tubulin alpha chain

XT: Tubulin beta chain

XU: Tubulin alpha chain

XV: Tubulin beta chain

XW: Tubulin alpha chain

XX: Tubulin beta chain

XY: Tubulin alpha chain

XZ: Tubulin beta chain

YA: Tubulin alpha chain

YB: Tubulin beta chain

YC: Tubulin alpha chain

YD: Tubulin beta chain

YE: Tubulin alpha chain

YF: Tubulin beta chain

YG: Tubulin alpha chain

YH: Tubulin beta chain

YJ: Tubulin alpha chain

YK: Tubulin beta chain

YL: Tubulin alpha chain

YM: Tubulin beta chain

YN: Tubulin alpha chain

YO: Tubulin beta chain

YP: Tubulin alpha chain

YQ: Tubulin beta chain

YR: Tubulin alpha chain

YS: Tubulin beta chain

YT: Tubulin alpha chain

YU: Tubulin beta chain

YV: Tubulin alpha chain

YW: Tubulin beta chain

YX: Tubulin alpha chain

YY: Tubulin beta chain

YZ: Tubulin alpha chain

ZA: Tubulin beta chain

ZB: Tubulin alpha chain

ZC: Tubulin beta chain

ZD: Tubulin alpha chain

ZE: Tubulin beta chain

ZF: Tubulin alpha chain

ZG: Tubulin beta chain

ZH: Tubulin alpha chain

ZI: Tubulin beta chain

ZJ: Tubulin alpha chain

ZK: Tubulin beta chain

ZL: Tubulin alpha chain

ZM: Tubulin beta chain

ZN: Tubulin alpha chain

ZO: Tubulin beta chain

ZP: Tubulin alpha chain

ZQ: Tubulin beta chain

ZR: Tubulin alpha chain

ZS: Tubulin beta chain

ZT: Tubulin alpha chain

ZU: Tubulin beta chain

ZV: Tubulin alpha chain

ZW: Tubulin beta chain

ZX: Tubulin alpha chain

ZY: Tubulin beta chain

ZZ: Tubulin alpha chain

hetero molecules

Summary:

• 63.7 MDa, 1166 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	63,727,105	2291
Polymers	63,369,452	1166
Non-polymers	357,654	1125
Water	0	0

1

Summary:

• Idetical with deposited unit
• defined by author

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555		1

Components

EF-hand domain-containing ... , 9 types, 26 molecules 0A 0B 0C 0D 0E 0F 0G 0H 0I 1H 1I 1J 1K 1R 1S 1W 1X 2A 2B 2C 2G 2H 2I 3J 3K 3L

#1: Protein

0A 0B 0C 0D 0E 0F
EF-hand domain-containing family member C2

Details:

Mass: 86973.781 Da / Num. of mol.: 6 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q387A9

#2: Protein

0G 0H 0I EF-hand domain-containing family member C2

Details:

Mass: 88366.906 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZX1

#19: Protein

1H 1I EF-hand domain-containing protein

Details:

Mass: 47731.914 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q581Q7

#20: Protein

1J 1K EF-hand domain-containing protein

Details:

Mass: 48071.531 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q388A8

#24: Protein

1R 1S EF-hand domain-containing protein

Details:

Mass: 30499.936 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57XA3

#26: Protein

1W 1X EF-hand domain-containing protein

Details:

Mass: 61839.383 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383Y5

#46: Protein

2A 2B 2C EF-hand domain-containing protein

Details:

Mass: 34213.496 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38F14

#48: Protein

2G 2H 2I EF-hand domain-containing protein

Details:

Mass: 36100.395 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38AR7

#65: Protein

3J 3K 3L EF-hand domain-containing protein

Details:

Mass: 107505.344 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385C3

Protein , 87 types, 933 molecules 0J 0K 0L 0M 0N 0O 0P 0Q 0R 0S 0T 0Y 0Z 1A 1B 0a 0b 0c 0d 0e 0f 0g 0h 0i 0j 0k 0l 0m 0n 0o ...

#3: Protein

0J 0K 0L Rib72 protein-like protein

Details:

Mass: 82953.188 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38AJ5

#4: Protein

0M 0N CMF34/CARP4

Details:

Mass: 90251.391 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)

#5: Protein

0O 0P 0Q 0R 0S 0T
Flagellar protofilament ribbon protein, putative

Details:

Mass: 46956.750 Da / Num. of mol.: 6 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57UY7

#7: Protein

0Y 0Z 1A 1B
Meiosis-specific nuclear structural protein 1

Details:

Mass: 52699.703 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q587B8

#8: Protein

0a 0b 0c 0d 0e 0f
KIAA1430 homologue

Details:

Mass: 19570.469 Da / Num. of mol.: 6 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38AV7

#9: Protein

0g 0h 0i 0j 0k 0l 0m 0n 0o 0p
Starmaker

Details:

Mass: 95914.680 Da / Num. of mol.: 10 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZC1

#10: Protein

0q LIM zinc-binding domain-containing protein

Details:

Mass: 129057.336 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q586W1

#11: Protein

0r 0s Sperm-tail PG-rich repeat

Details:

Mass: 51973.930 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38DR1

#12: Protein

0t MOP84A

Details:

Mass: 83951.594 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZL7

#21: Protein

1L 1M Cyclic nucleotide-binding domain-containing protein

Details:

Mass: 40593.711 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q387J0

#22: Protein

1N 1O TbMIP23

Details:

Mass: 23452.754 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57XG0

#23: Protein

1P 1Q FAP141

Details:

Mass: 14631.420 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38BT0

#25: Protein

1T 1U 1V Calpain-like cysteine peptidase, putative

Details:

Mass: 33172.227 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q381R3

#27: Protein

1Y 1Z Calcium-binding protein, putative

Details:

Mass: 41708.535 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q583R2

#30: Protein

1c CMF5

Details:

Mass: 150701.219 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q586S4

#31: Protein

1d CMF6

Details:

Mass: 136693.719 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57UW5

#32: Protein

1e CCDC113/CCDC96 coiled-coil domain-containing protein

Details:

Mass: 48233.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57X67

#34: Protein

1g DRC1

Details:

Mass: 76937.523 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38AH6

#37: Protein

1j Trypanin-related protein, putative

Details:

Mass: 54270.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38CZ5

#38: Protein

1k Trypanin

Details:

Mass: 54023.203 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38AX1

#40: Protein

1m Transglutaminase-like domain-containing protein

Details:

Mass: 153777.312 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57VW3

#41: Protein

1n DRC9

Details:

Mass: 63788.734 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383N6

#42: Protein

1o Dynein regulatory complex protein 10

Details:

Mass: 50633.656 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57UH4

#43: Protein

1p ATPase AAA-type core domain-containing protein

Details:

Mass: 103013.508 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386H5

#47: Protein

2D 2E 2F Peptidyl-prolyl cis-trans isomerase / PPIase

Details:

Mass: 30680.295 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: A0A1G4I9Z4, peptidylprolyl isomerase

#49: Protein

2J 2K 2L FAP107/MC11

Details:

Mass: 28127.812 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q583W1

#51: Protein

2P 2Q FAP95/MC6

Details:

Mass: 20151.945 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q389U0

#52: Protein

2R 2S 2T FAP129

Details:

Mass: 42654.492 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57Z34

#54: Protein

2X 2Y 2Z 3D
FAP21

Details:

Mass: 46442.445 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383S0

#55: Protein

2a 2b 2c 2d
RSP1

Details:

Mass: 37063.328 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZM3

#56: Protein

2e 2f 2g 2h
RSP2

Details:

Mass: 57662.398 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZW1

#60: Protein

2u RSP8

Details:

Mass: 47692.613 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZK0

#61: Protein

2v 2w 2x 2y
Radial spoke head protein 9 homolog

Details:

Mass: 33891.391 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386Q6

#63: Protein

3A 3B 3C Flagellar associated protein

Details:

Mass: 45935.605 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385B6

#64: Protein

3E 3F 3G 3H 3I
TbRib26b

Details:

Mass: 26226.729 Da / Num. of mol.: 5 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q384Q9

#66: Protein

3M 3N 3O 3P 3Q 3R 3S 3T
PACRGA

Details:

Mass: 33834.922 Da / Num. of mol.: 8 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q582U4

#67: Protein

3U 3V 3W 3X 3Y
PACRGB

Details:

Mass: 35401.648 Da / Num. of mol.: 5 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38E08

#69: Protein

3d 3e 3f 3g
Phosphatidylinositol 4-phosphate 5-kinase

Details:

Mass: 92368.773 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57XR1

#70: Protein

3h 3i Cyclophilin type peptidyl-prolyl cis-trans isomerase, putative

Details:

Mass: 35820.371 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38FI6, peptidylprolyl isomerase

#71: Protein

3j RSP14

Details:

Mass: 43522.430 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385G1

#73: Protein

3l 3m 3o 3p
Chaperone protein DNAJ, putative

Details:

Mass: 35364.879 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q381S2

#74: Protein

3q 3r 3s 3t
Nucleoside diphosphate kinase

Details:

Mass: 29018.053 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386D5

#78: Protein

4o 4p Cytochrome b5 domain-containing protein 1

Details:

Mass: 25618.709 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38CH5

#80: Protein

4s 4t VWFA domain-containing protein

Details:

Mass: 91290.422 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57X62

#81: Protein

4u 4v TbRSP63

Details:

Mass: 63201.164 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38FW7

#82: Protein

4w IQ and ubiquitin-like domain-containing protein

Details:

Mass: 79406.180 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57Y89

#83: Protein

4x Calmodulin

Details:

Mass: 16853.572 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q382N3

#84: Protein

4y MORN repeat-containing protein 3

Details:

Mass: 81249.883 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q388E6

#87: Protein

5C 5D 5E 5F 5G 5H 5I 5J
MC4

Details:

Mass: 40548.527 Da / Num. of mol.: 8 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57XA7

#89: Protein

5L 5M CCDC81 HU domain-containing protein

Details:

Mass: 29888.291 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383J9

#90: Protein

5N 5O 5P 5Q 5R
MC5

Details:

Mass: 30760.701 Da / Num. of mol.: 5 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385S6

#92: Protein

5a TbRSP62

Details:

Mass: 62994.465 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386B4

#98: Protein

5g Leucine-rich repeat-containing protein 51

Details:

Mass: 69581.281 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q580G7

#99: Protein

5h 5i IDAf-alpha

Details:

Mass: 527784.688 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)

#101: Protein

5l Dynein axonemal intermediate chain 4 / WD repeat-containing protein 78

Details:

Mass: 94746.680 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q586W0

#102: Protein

5m IC140

Details:

Mass: 90521.164 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386T2

#103: Protein

5n IC97/Casc1 N-terminal domain-containing protein

Details:

Mass: 78862.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZZ4

#104: Protein

5o FAP120

Details:

Mass: 46515.723 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q587C7

#110: Protein

6R 6S 6T 6U 6V 6W 6X 6Y 6Z 7A 7B 7C 7D 7E 7F 7G 7H 7I 7J ...
STOP axonemal protein

Details:

Mass: 30090.230 Da / Num. of mol.: 21 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57YM5

#116: Protein

6f 6g Actin A

Details:

Mass: 41942.711 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38E94

#117: Protein

6h Actin-like protein, putative

Details:

Mass: 43532.371 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383R8

#118: Protein

6i Actin, putative

Details:

Mass: 46810.180 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38F11

#119: Protein

6j Actin, putative

Details:

Mass: 47607.672 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386C6

#121: Protein

6n 33 kDa inner dynein arm light chain, axonemal, putative

Details:

Mass: 42154.117 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q389G1

#124: Protein

6q Protein TAX-1 / TbTAX-1

Details:

Mass: 40739.570 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38DX5

#127: Protein

6t Centrin, putative

Details:

Mass: 18827.430 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38AA2

#128: Protein

6u Centrin, putative

Details:

Mass: 20737.582 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q382E7

#129: Protein

7L 7M MC8

Details:

Mass: 31647.955 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386N3

#130: Protein

7N 7O 7P MC3

Details:

Mass: 37327.500 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38AQ1

#131: Protein

7Q 7R 7S FAP90

Details:

Mass: 21434.402 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZR3

#132: Protein

7T 7U 8L PBP36

Details:

Mass: 36147.027 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57UM2

#136: Protein

7c 8c 9c TbDC65

Details:

Mass: 65348.320 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q585P4

#137: Protein

7d 8d 9d TbDC13

Details:

Mass: 13145.440 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57UW1

#138: Protein

7g 8g 9g Dynein, putative

Details:

Mass: 68203.023 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q382A7

#139: Protein

7h 8h 9h Dynein intermediate chain, putative

Details:

Mass: 73388.812 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385R8

#142: Protein

7s 8s 9a 9s
Outer dynein arm docking complex

Details:

Mass: 76060.531 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57VZ5

#143: Protein

7t 8t 9b 9t
ODAD1 central coiled coil region domain-containing protein

Details:

Mass: 65236.535 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q582I1

#145: Protein

8M 8N 8O 8P 8Q 8R 8S 8T
PON3

Details:

Mass: 46311.688 Da / Num. of mol.: 8 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q388F3

#146: Protein

8U 8V 8W 8X 8Y 8Z 9A 9B
PON4

Details:

Mass: 37083.625 Da / Num. of mol.: 8 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q388F2

#147: Protein

9C 9D 9E MC7

Details:

Mass: 37334.016 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57YM4

#148: Protein

9F 9G 9H 9I
FAP96C/MC15

Details:

Mass: 39428.738 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57V66

#149: Protein

9J 9K 9L FAP96B

Details:

Mass: 33435.266 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q384B8

#150: Protein

9M 9N 9O 9P 9Q 9R 9S
MOP23A

Details:

Mass: 23471.566 Da / Num. of mol.: 7 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q582N6

#151: Protein

9T 9U 9V MOP23B

Details:

Mass: 22494.564 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383L7

#152: Protein

9W 9X 9Y MOP23C

Details:

Mass: 19407.205 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q580D2

#153: Protein

AA AC AE AG AI AK AM AO AQ AS AU AW AY Aa Ac Ae Ag Ai Ak ...
Tubulin beta chain

Details:

Mass: 49747.926 Da / Num. of mol.: 339 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q4GYY6

#154: Protein

AB AD AF AH AJ AL AN AP AR AT AV AX AZ Ab Ad Af Ah Aj Al ...
Tubulin alpha chain

Details:

Mass: 49840.094 Da / Num. of mol.: 342 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q4GYY5

Cilia- and flagella-associated protein ... , 14 types, 43 molecules 0U 0V 0W 0X 0z 1a 1b 1f 1r 1t 3Z 4A 4B 4C 4D 4E 4F 4G 4H 4I 4J 4K 4L 4M 4N 4O 4P 4Q 4R 4S ...

#6: Protein

0U 0V 0W 0X
Cilia- and flagella-associated protein 53

Details:

Mass: 58689.828 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57VE0

#16: Protein

0z Cilia- and flagella-associated protein 299

Details:

Mass: 35169.812 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q382P6

#28: Protein

1a Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein

Details:

Mass: 99966.516 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383I9

#29: Protein

1b Cilia- and flagella-associated protein 58 central coiled coil domain-containing protein

Details:

Mass: 119009.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q587G2

#33: Protein

1f Cilia- and flagella-associated protein 263

Details:

Mass: 56808.766 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57UP0

#44: Protein

1r Cilia- and flagella-associated protein 43

Details:

Mass: 165164.141 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q580P9

#45: Protein

1t Cilia- and flagella-associated protein 44 / Component of motile flagella 7

Details:

Mass: 199488.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57WH1

#68: Protein

3Z 4A 4B 4C 4D 4E 4F 4G 4H 4I 4J 4K 4L 4M 4N
Cilia- and flagella-associated protein 20

Details:

Mass: 34593.465 Da / Num. of mol.: 15 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38C25

#76: Protein

4O 4P 4Q 4R 4S 4T 4U
Cilia- and flagella-associated protein 52

Details:

Mass: 67770.891 Da / Num. of mol.: 7 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q384Q0

#85: Protein

4z Cilia- and flagella-associated protein 206

Details:

Mass: 72146.523 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57VI3

#88: Protein

5K 5S 5T 5U 5V 5W 5X
Cilia- and flagella-associated protein 45

Details:

Mass: 58198.273 Da / Num. of mol.: 7 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57UZ3

#93: Protein

5b Cilia- and flagella-associated protein 91 / Flagellar-associated protein 91

Details:

Mass: 80153.539 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386N1

#94: Protein

5c Cilia- and flagella-associated protein 251

Details:

Mass: 112677.266 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZE4

#95: Protein

5d Cilia- and flagella-associated protein 61 N-terminal domain-containing protein

Details:

Mass: 152687.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57WR9

Leucine-rich repeat protein ... , 4 types, 5 molecules 0u 1l 3k 4q 4r

#13: Protein

0u Leucine-rich repeat protein (LRRP)

Details:

Mass: 82112.438 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q4GYG0

#39: Protein

1l Leucine-rich repeat protein (LRRP)

Details:

Mass: 53740.465 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q582D8

#72: Protein

3k Leucine-rich repeat protein (LRRP)

Details:

Mass: 39438.836 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q581X0

#79: Protein

4q 4r Leucine-rich repeat protein (LRRP)

Details:

Mass: 95867.430 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385D2

Coiled-coil domain-containing protein ... , 2 types, 4 molecules 0v 0w 0x 0y

#14: Protein

0v 0w Coiled-coil domain-containing protein 39

Details:

Mass: 100037.195 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57WL0

#15: Protein

0x 0y Coiled-coil domain-containing protein 40

Details:

Mass: 101298.609 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q389L4

Nucleoside diphosphate kinase, ... , 2 types, 5 molecules 1C 1D 1G 1E 1F

#17: Protein

1C 1D 1G Nucleoside diphosphate kinase, putative

Details:

Mass: 37147.473 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38DQ0, nucleoside-diphosphate kinase

#18: Protein

1E 1F Nucleoside diphosphate kinase, putative

Details:

Mass: 38993.656 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q581Q9, nucleoside-diphosphate kinase

Dynein regulatory complex subunit ... , 2 types, 2 molecules 1h 1i

#35: Protein

1h Dynein regulatory complex subunit 2

Details:

Mass: 58770.551 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q384U1

#36: Protein

1i Dynein regulatory complex subunit 3

Details:

Mass: 64946.977 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q586H5

T. brucei spp.-specific ... , 2 types, 6 molecules 2M 2N 2O 2U 2V 2W

#50: Protein

2M 2N 2O T. brucei spp.-specific protein

Details:

Mass: 28844.326 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38EE4

#53: Protein

2U 2V 2W T. brucei spp.-specific protein

Details:

Mass: 46540.539 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q384L6

Radial spoke protein ... , 2 types, 8 molecules 2i 2j 2k 2l 2z 3a 3b 3c

#57: Protein

2i 2j 2k 2l
Radial spoke protein 3, putative

Details:

Mass: 39440.613 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q387D7

#62: Protein

2z 3a 3b 3c
Radial spoke protein RSP9

Details:

Mass: 37558.008 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57YF0

Flagellar radial spoke ... , 2 types, 8 molecules 2m 2n 2o 2p 2q 2r 2s 2t

#58: Protein

2m 2n 2o 2p
Flagellar radial spoke component, putative

Details:

Mass: 66964.766 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385W9

#59: Protein

2q 2r 2s 2t
Flagellar radial spoke protein-like, putative

Details:

Mass: 61053.602 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57WA5

Dynein light ... , 7 types, 60 molecules 3u 3v 3w 3x 3y 3z 4a 4b 4c 4d 4e 4f 4g 4h 4i 4j 4k 4l 4m 4n 5s 5t 5u 5v 5w 5x 7k 7n 7o 7p ...

#75: Protein

3u 3v 3w 3x 3y 3z 4a 4b 4c 4d 4e 4f 4g 4h 4i 4j 4k 4l 4m ...
Dynein light chain

Details:

Mass: 10442.935 Da / Num. of mol.: 38 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q4FKD8

#105: Protein

5p 7i 8i 9i
Dynein light chain, putative

Details:

Mass: 14674.654 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38DD2

#106: Protein

5q 7l 8l 9l
Dynein light chain roadblock

Details:

Mass: 12839.684 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q388T6

#107: Protein

5r 7m 8m 9m
Dynein light chain roadblock

Details:

Mass: 11143.684 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38D18

#108: Protein

5y 7q 8q 9q
Dynein light chain, putative

Details:

Mass: 13287.048 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386E6

#140: Protein

7j 8j 9j Dynein light chain

Details:

Mass: 13047.076 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38BD2

#141: Protein

7r 8r 9r Dynein light chain

Details:

Mass: 14118.888 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38AT9

Enkurin domain-containing ... , 4 types, 23 molecules 4V 4W 4X 4Y 4Z 5A 5B 7V 7W 7X 7Y 7Z 8A 8B 8C 8D 8E 8F 8G 8H 8I 8J 8K

#77: Protein

4V 4W 4X 4Y 4Z
Enkurin domain-containing protein

Details:

Mass: 30264.260 Da / Num. of mol.: 5 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385T0

#86: Protein

5A 5B Enkurin domain-containing protein

Details:

Mass: 42281.645 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q4GZB9

#133: Protein

7V 7W 7X 7Y 7Z 8A 8B 8C
Enkurin domain-containing protein

Details:

Mass: 31393.762 Da / Num. of mol.: 8 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q583F4

#144: Protein

8D 8E 8F 8G 8H 8I 8J 8K
Enkurin domain-containing protein

Details:

Mass: 30823.730 Da / Num. of mol.: 8 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q583F5

Trichohyalin-plectin-homology domain-containing ... , 2 types, 19 molecules 5Y 5Z 6A 6B 6C 6D 6E 6F 6G 6H 6I 6J 6K 6L 6M 6N 6O 6P 6Q

#91: Protein

5Y 5Z 6A Trichohyalin-plectin-homology domain-containing protein

Details:

Mass: 61310.469 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386E1

#109: Protein

6B 6C 6D 6E 6F 6G 6H 6I 6J 6K 6L 6M 6N 6O 6P 6Q
Trichohyalin-plectin-homology domain-containing protein

Details:

Mass: 41736.418 Da / Num. of mol.: 16 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383H7

DUF4200 domain-containing ... , 2 types, 2 molecules 5e 5f

#96: Protein

5e DUF4200 domain-containing protein

Details:

Mass: 35693.316 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385W2

#97: Protein

5f DUF4200 domain-containing protein

Details:

Mass: 77462.977 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q381R6

Dynein heavy chain, ... , 3 types, 7 molecules 5j 5k 6c 7a 7e 8e 9e

#100: Protein

5j 5k Dynein heavy chain, putative

Details:

Mass: 538056.500 Da / Num. of mol.: 2 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57YY8

#113: Protein

6c Dynein heavy chain, putative

Details:

Mass: 468637.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57VT8

#134: Protein

7a 7e 8e 9e
Dynein heavy chain, putative

Details:

Mass: 532087.188 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57WX6

Dynein arm light chain, axonemal, ... , 3 types, 5 molecules 6k 6l 6m 6o 6p

#120: Protein

6k 6l 6m Dynein arm light chain, axonemal, putative

Details:

Mass: 27409.299 Da / Num. of mol.: 3 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q385L7

#122: Protein

6o Dynein arm light chain, axonemal, putative / Dynein light chain / putative

Details:

Mass: 31228.834 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q4FKF9

#123: Protein

6p Dynein arm light chain, axonemal, putative

Details:

Mass: 34412.762 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57VS8

Tetratricopeptide repeat ... , 2 types, 2 molecules 6r 6s

#125: Protein

6r Tetratricopeptide repeat protein 29 / TbTTC29

Details:

Mass: 53568.684 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q57ZB2

#126: Protein

6s Tetratricopeptide repeat protein

Details:

Mass: 51283.645 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q585T0

Antibody , 5 types, 8 molecules 6a 6b 6d 6e 7b 7f 8f 9f

#111: Antibody

6a Dynein heavy chain, putative

Details:

Mass: 486310.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q585U4

#112: Antibody

6b Dynein heavy chain, putative

Details:

Mass: 475264.562 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q38B68

#114: Antibody

6d Dynein heavy chain, putative

Details:

Mass: 480844.219 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q383F6

#115: Antibody

6e Dynein heavy chain, putative

Details:

Mass: 474397.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q384K3

#135: Antibody

7b 7f 8f 9f
Dynein heavy chain, putative

Details:

Mass: 531832.562 Da / Num. of mol.: 4 / Source method: isolated from a natural source
Source: (natural) Trypanosoma brucei brucei TREU927 (eukaryote)
References: UniProt: Q386I6

Non-polymers , 6 types, 1125 molecules

#155: Chemical

0q-1301 0q-1302 5C-401 5C-402 5E-501 5F-401 5F-402 5H-501 5I-501 5I-502 5L-501 5M-501 7V-501 7V-502 7W-501 7W-502 7X-501 7X-502 7X-503 7Y-301 ...
ChemComp-ZN / ZINC ION

Details:

Mass: 65.409 Da / Num. of mol.: 70 / Source method: obtained synthetically / Formula: Zn

#156: Chemical

7a-4701 7a-4702 7a-4704 7b-4701 7b-4702 7b-4703 7e-4702 7e-4703 7e-4704 7f-4702 7f-4703 7f-4704 8e-4702 8e-4703 8e-4704 8f-4702 8f-4703 8f-4704 9e-5001 9e-5003 ...
ChemComp-ATP / ADENOSINE-5'-TRIPHOSPHATE

Details:

Mass: 507.181 Da / Num. of mol.: 24 / Source method: obtained synthetically / Formula: C₁₀H₁₆N₅O₁₃P₃ / Feature type: SUBJECT OF INVESTIGATION / Comment: ATP, energy-carrying molecule*YM

#157: Chemical

7a-4703 7b-4704 7e-4701 7f-4701 8e-4701 8f-4701 9e-5002 9f-4702
ChemComp-ADP / ADENOSINE-5'-DIPHOSPHATE

Details:

Mass: 427.201 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C₁₀H₁₅N₅O₁₀P₂ / Feature type: SUBJECT OF INVESTIGATION / Comment: ADP, energy-carrying molecule*YM

#158: Chemical

AA-501 AC-501 AE-501 AG-501 AI-501 AK-501 AM-501 AO-501 AQ-501 AS-501 AU-501 AW-501 AY-501 Aa-501 Ac-501 Ae-501 Ag-501 Ai-501 Ak-501 Am-501 ...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE

Details:

Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 339 / Source method: obtained synthetically / Formula: C₁₀H₁₅N₅O₁₁P₂ / Comment: GDP, energy-carrying molecule*YM

#159: Chemical

AB-601 AD-502 AF-502 AH-502 AJ-502 AL-601 AN-502 AP-601 AR-601 AT-502 AV-502 AX-502 AZ-502 Ab-502 Ad-601 Af-502 Ah-502 Aj-502 Al-601 An-601 ...
ChemComp-MG / MAGNESIUM ION

Details:

Mass: 24.305 Da / Num. of mol.: 342 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION

#160: Chemical

AB-602 AD-501 AF-501 AH-501 AJ-501 AL-602 AN-501 AP-602 AR-602 AT-501 AV-501 AX-501 AZ-501 Ab-501 Ad-602 Af-501 Ah-501 Aj-501 Al-602 An-602 ...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE

Details:

Mass: 523.180 Da / Num. of mol.: 342 / Source method: obtained synthetically / Formula: C₁₀H₁₆N₅O₁₄P₃ / Comment: GTP, energy-carrying molecule*YM

Details

• Has ligand of interest: Y
• Has protein modification: Y

Experimental details

Experiment

• Experiment: Method: ELECTRON MICROSCOPY
• EM experiment: Aggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

Sample preparation

• Component: Name: Trypanosoma brucei flagella / Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: #1-#154 / Source: NATURAL
• Source (natural): Organism: Trypanosoma brucei brucei TREU927 (eukaryote)
• Buffer solution: pH: 7.4
• Specimen: Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
• Specimen support: Grid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R2/1
• Vitrification: Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K

Electron microscopy imaging

• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company
• Microscopy: Model: TFS KRIOS
• Electron gun: Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
• Electron lens: Mode: BRIGHT FIELD / Nominal magnification: 81000 X / Nominal defocus max: 2400 nm / Nominal defocus min: 1600 nm / Cs: 2.7 mm / C2 aperture diameter: 50 µm
• Specimen holder: Cryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
• Image recording: Average exposure time: 2 sec. / Electron dose: 50 e/Ų / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Num. of grids imaged: 2 / Num. of real images: 106694

Processing

EM software

ID	Name	Version	Category
2	SerialEM	4.1	image acquisition
9	RELION	3.1	initial Euler assignment
10	RELION	3.1	final Euler assignment
11	RELION	3.1	classification
12	cryoSPARC	4.1	3D reconstruction
13	PHENIX	1.19	model refinement

• CTF correction: Type: PHASE FLIPPING ONLY
• Particle selection: Num. of particles selected: 7830978
• Symmetry: Point symmetry: C₁ (asymmetric)
• 3D reconstruction: Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 244497 / Num. of class averages: 1 / Symmetry type: POINT
• Atomic model building: Space: REAL