EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and infection dynamics of mycobacteriophage Bxb1.
ジャーナル・号・ページ	Cell, Vol. 188, Issue 11, Page 2925-2942.e17, Year 2025
掲載日	2025年5月29日
著者	Krista G Freeman / Sudipta Mondal / Lourriel S Macale / Jennifer Podgorski / Simon J White / Benjamin H Silva / Valery Ortiz / Alexis Huet / Ronelito J Perez / Joemark T Narsico / Meng-Chiao Ho / Deborah Jacobs-Sera / Todd L Lowary / James F Conway / Donghyun Park / Graham F Hatfull /
PubMed 要旨	Mycobacteriophage Bxb1 is a well-characterized virus of Mycobacterium smegmatis with double-stranded DNA and a long, flexible tail. Mycobacteriophages show considerable potential as therapies for ...Mycobacteriophage Bxb1 is a well-characterized virus of Mycobacterium smegmatis with double-stranded DNA and a long, flexible tail. Mycobacteriophages show considerable potential as therapies for Mycobacterium infections, but little is known about the structural details of these phages or how they bind to and traverse the complex Mycobacterium cell wall. Here, we report the complete structure and atomic model of phage Bxb1, including the arrangement of immunodominant domains of both the capsid and tail tube subunits, as well as the assembly of the protein subunits in the tail-tip complex. The structure contains protein assemblies with 3-, 5-, 6-, and 12-fold symmetries, which interact to satisfy several symmetry mismatches. Cryoelectron tomography of phage particles bound to M. smegmatis reveals the structural transitions that occur for free phage particles to bind to the cell surface and navigate through the cell wall to enable DNA transfer into the cytoplasm.
リンク	Cell / PubMed:40239650 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	2.45 - 26.0 Å
構造データ	EMDB-46656: Mycobacteriophage Bxb1 tail tip - Consensus C3 map 手法: EM (単粒子) / 解像度: 2.45 Å EMDB-46657: Mycobacteriophage Bxb1 tail tip - Tail spike C1 local refinement 手法: EM (単粒子) / 解像度: 2.85 Å EMDB-46658: Mycobacteriophage Bxb1 tail tip - Tail wings C1 local refinement 手法: EM (単粒子) / 解像度: 3.98 Å EMDB-46659: Mycobacteriophage Bxb1 tail tip - Tail collar and fibers C1 local refinement 手法: EM (単粒子) / 解像度: 3.65 Å 46661 9d93 EMDB-46661, PDB-9d93: Mycobacteriophage Bxb1 tail tip - Composite map and model 手法: EM (単粒子) / 解像度: 2.85 Å EMDB-46663: Mycobacteriophage Bxb1 portal and connector assembly - C3 consensus refinement 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-46666: Mycobacteriophage Bxb1 portal and connector assembly - Portal and head-to-tail adaptor C12 local refinement 手法: EM (単粒子) / 解像度: 2.69 Å EMDB-46667: Mycobacteriophage Bxb1 portal and connector assembly - Head-to-tail stopper and tail terminator C6 local refinement 手法: EM (単粒子) / 解像度: 2.68 Å EMDB-46668: Mycobacteriophage Bxb1 portal and connector assembly - Tail tube C3 local refinement 手法: EM (単粒子) / 解像度: 3.06 Å 46669 9d94 EMDB-46669, PDB-9d94: Mycobacteriophage Bxb1 portal and connector assembly - Composite map and model 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-46673: Mycobacteriophage Bxb1 tail tube segment - C1 consensus refinement 手法: EM (単粒子) / 解像度: 3.11 Å EMDB-46674: Mycobacteriophage Bxb1 tail tube segment - Core C1 local refinement 手法: EM (単粒子) / 解像度: 2.99 Å EMDB-46675: Mycobacteriophage Bxb1 tail tube segment - CTE domain C1 local refinement 手法: EM (単粒子) / 解像度: 4.88 Å 46676 9d9l EMDB-46676, PDB-9d9l: Mycobacteriophage Bxb1 tail tube segment - Composite map and model 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-46678: Mycobacteriophage Bxb1 C1 Capsid and Portal - C1 consensus map 手法: EM (単粒子) / 解像度: 3.44 Å EMDB-46679: Mycobacteriophage Bxb1 C1 Capsid and Portal - Portal C1 local refinement 手法: EM (単粒子) / 解像度: 3.31 Å 46681 9d9w EMDB-46681, PDB-9d9w: Mycobacteriophage Bxb1 C1 Capsid and Portal - Composite map and model 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-46682: Mycobacteriophage Bxb1 Capsid - Icosahedral Consensus Map 手法: EM (単粒子) / 解像度: 2.71 Å EMDB-46684: Mycobacteriophage Bxb1 Capsid - Penton C1 local refinement 手法: EM (単粒子) / 解像度: 3.17 Å 46685 9d9x EMDB-46685, PDB-9d9x: Mycobacteriophage Bxb1 Capsid - Composite map and model 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-46687: Mycobacteriophage BaDAS-1 Icosahedral Map 手法: EM (単粒子) / 解像度: 2.67 Å EMDB-46702: Subtomogram averaging of Bxb1 phage capsid (DNA-filled) during the infection state 手法: EM (サブトモグラム平均) / 解像度: 11.65 Å EMDB-46703: Subtomogram averaging of Bxb1 phage tail tube (DNA-filled) during the infection state 手法: EM (サブトモグラム平均) / 解像度: 7.0 Å EMDB-46704: Subtomogram averaging of Bxb1 phage capsid (emptied) during the infection state 手法: EM (サブトモグラム平均) / 解像度: 12.5 Å EMDB-46705: Subtomogram averaging of Bxb1 phage tail tube (emptied) during the infection state 手法: EM (サブトモグラム平均) / 解像度: 22.0 Å EMDB-46706: Subtomogram averaging of Bxb1 phage portal (DNA-filled) during the infection state 手法: EM (サブトモグラム平均) / 解像度: 23.0 Å EMDB-46707: Subtomogram averaging of Bxb1 phage portal (emptied) during the infection state 手法: EM (サブトモグラム平均) / 解像度: 26.0 Å EMDB-46723: Subtomogram averaging of Bxb1 phage tail tip complex during the infection state 手法: EM (サブトモグラム平均) / 解像度: 15.0 Å EMDB-48066: Subtomogram averaging of Bxb1 phage tail tip (infection state) 手法: EM (サブトモグラム平均) / 解像度: 8.48 Å EMDB-48067: Subtomogram averaging of Bxb1 phage tail tip (infection state, extra density within cell wall) 手法: EM (サブトモグラム平均) / 解像度: 24.0 Å
由来	mycobacterium phage bxb1 (ファージ)
キーワード	VIRAL PROTEIN / Bacteriophage / tail tip / portal / tail tube / VIRUS / Mycobacteriophage Bxb1 / capsid