+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-46661 | |||||||||||||||||||||||||||
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Title | Mycobacteriophage Bxb1 tail tip - Composite map and model | |||||||||||||||||||||||||||
Map data | Mycobacteriophage Bxb1 tail tip - Composite map and model | |||||||||||||||||||||||||||
Sample |
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Keywords | Bacteriophage / tail tip / VIRAL PROTEIN | |||||||||||||||||||||||||||
Function / homology | Function and homology information | |||||||||||||||||||||||||||
Biological species | Mycobacterium phage Bxb1 (virus) | |||||||||||||||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 2.85 Å | |||||||||||||||||||||||||||
Authors | Freeman KG / White SJ / Huet A / Conway JF | |||||||||||||||||||||||||||
Funding support | United States, Taiwan, 8 items
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Citation | Journal: To Be Published Title: Structure and infection dynamics of mycobacteriophage Bxb1 Authors: Freeman KG / Mondal S / Macale LS / Podgorski J / White SJ / Silva B / Ortiz V / Huet A / Narsico JT / Ho MC / Jacobs-Sera D / Lowary TL / Conway JF / Park D / Hatfull GF | |||||||||||||||||||||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_46661.map.gz | 352.9 MB | EMDB map data format | |
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Header (meta data) | emd-46661-v30.xml emd-46661.xml | 25.7 KB 25.7 KB | Display Display | EMDB header |
Images | emd_46661.png | 99.7 KB | ||
Filedesc metadata | emd-46661.cif.gz | 8.8 KB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-46661 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-46661 | HTTPS FTP |
-Validation report
Summary document | emd_46661_validation.pdf.gz | 419 KB | Display | EMDB validaton report |
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Full document | emd_46661_full_validation.pdf.gz | 418.5 KB | Display | |
Data in XML | emd_46661_validation.xml.gz | 7.7 KB | Display | |
Data in CIF | emd_46661_validation.cif.gz | 9 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-46661 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-46661 | HTTPS FTP |
-Related structure data
Related structure data | 9d93MC 9d94C 9d9lC 9d9wC 9d9xC 46656 46657 46658 46659 M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_46661.map.gz / Format: CCP4 / Size: 421.9 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Annotation | Mycobacteriophage Bxb1 tail tip - Composite map and model | ||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.07 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Sample components
+Entire : Mycobacterium phage Bxb1
+Supramolecule #1: Mycobacterium phage Bxb1
+Macromolecule #1: Major tail protein
+Macromolecule #2: Minor tail protein
+Macromolecule #3: Minor tail protein
+Macromolecule #4: Minor tail protein
+Macromolecule #5: Tapemeasure protein
+Macromolecule #6: Minor tail protein
+Macromolecule #7: Minor tail protein
+Macromolecule #8: Minor tail protein
+Macromolecule #9: Minor tail protein
+Macromolecule #10: Minor tail protein
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 10 mg/mL | |||||||||||||||
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Buffer | pH: 7.5 Component:
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 283.15 K / Instrument: FEI VITROBOT MARK IV |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K3 (6k x 4k) / Average electron dose: 30.0 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.8 µm |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: INSILICO MODEL |
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Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 2.85 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 22280 |
Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |