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-Structure paper
タイトル | Integrative structural analysis of Pseudomonas phage DEV reveals a genome ejection motor. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 8482, Year 2024 |
掲載日 | 2024年10月1日 |
著者 | Ravi K Lokareddy / Chun-Feng David Hou / Francesca Forti / Stephano M Iglesias / Fenglin Li / Mikhail Pavlenok / David S Horner / Michael Niederweis / Federica Briani / Gino Cingolani / |
PubMed 要旨 | DEV is an obligatory lytic Pseudomonas phage of the N4-like genus, recently reclassified as Schitoviridae. The DEV genome encodes 91 ORFs, including a 3398 amino acid virion-associated RNA polymerase ...DEV is an obligatory lytic Pseudomonas phage of the N4-like genus, recently reclassified as Schitoviridae. The DEV genome encodes 91 ORFs, including a 3398 amino acid virion-associated RNA polymerase (vRNAP). Here, we describe the complete architecture of DEV, determined using a combination of cryo-electron microscopy localized reconstruction, biochemical methods, and genetic knockouts. We built de novo structures of all capsid factors and tail components involved in host attachment. We demonstrate that DEV long tail fibers are essential for infection of Pseudomonas aeruginosa but dispensable for infecting mutants with a truncated lipopolysaccharide devoid of the O-antigen. We determine that DEV vRNAP is part of a three-gene operon conserved in 191 Schitoviridae genomes. We propose these three proteins are ejected into the host to form a genome ejection motor spanning the cell envelope. We posit that the design principles of the DEV ejection apparatus are conserved in all Schitoviridae. |
リンク | Nat Commun / PubMed:39353939 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.1 - 4.7 Å |
構造データ | EMDB-43629, PDB-8vxq: EMDB-44517, PDB-9bgm: EMDB-44518, PDB-9bgn: EMDB-44519, PDB-9bgo: EMDB-45776, PDB-9cod: |
由来 |
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キーワード | VIRAL PROTEIN / phage / bacteriophage / STRUCTURAL PROTEIN / outer membrane protein / gp73 / gp74 / DEV / portal / tail hub / tail tube / complex / gp75 / gp83 / gp80 / virion coat / gp77 / virion scaffolding / gp72 / ejection protein / gp53 |