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-Structure paper
タイトル | Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models. |
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ジャーナル・号・ページ | Nat Methods, Vol. 21, Issue 1, Page 37-40, Year 2024 |
掲載日 | 2023年11月16日 |
著者 | Muyuan Chen / Michael F Schmid / Wah Chiu / |
PubMed 要旨 | Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian ...Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures. |
リンク | Nat Methods / PubMed:37973972 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.5 - 3.1 Å |
構造データ | EMDB-41840, PDB-8u26: EMDB-41841, PDB-8u28: EMDB-41845, PDB-8u2c: |
化合物 | ChemComp-NAG: |
由来 |
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キーワード | MEMBRANE PROTEIN / GPCR / VIRUS / coronavirus spike protein / ABC transporter |