+Search query
-Structure paper
Title | Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models. |
---|---|
Journal, issue, pages | Nat Methods, Vol. 21, Issue 1, Page 37-40, Year 2024 |
Publish date | Nov 16, 2023 |
Authors | Muyuan Chen / Michael F Schmid / Wah Chiu / |
PubMed Abstract | Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian ...Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures. |
External links | Nat Methods / PubMed:37973972 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.5 - 3.1 Å |
Structure data | EMDB-41840, PDB-8u26: EMDB-41841, PDB-8u28: EMDB-41845, PDB-8u2c: |
Chemicals | ChemComp-NAG: |
Source |
|
Keywords | MEMBRANE PROTEIN / GPCR / VIRUS / coronavirus spike protein / ABC transporter |