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- PDB-8u26: Gaussian Mixture Models based single particle refinement - GPCR (... -

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Basic information

Entry
Database: PDB / ID: 8u26
TitleGaussian Mixture Models based single particle refinement - GPCR (Substance P bound to active human neurokinin 1 receptor in complex with miniGs399 from EMPIAR-10786)
Components
  • (Guanine nucleotide-binding protein ...) x 3
  • Nanobody 35
  • Protachykinin-1
  • Substance-P receptor
KeywordsMEMBRANE PROTEIN / GPCR
Function / homology
Function and homology information


substance P receptor activity / substance P receptor binding / aggressive behavior / positive regulation of corticosterone secretion / tachykinin receptor activity / positive regulation of flagellated sperm motility / insemination / Tachykinin receptors bind tachykinins / positive regulation of uterine smooth muscle contraction / detection of abiotic stimulus ...substance P receptor activity / substance P receptor binding / aggressive behavior / positive regulation of corticosterone secretion / tachykinin receptor activity / positive regulation of flagellated sperm motility / insemination / Tachykinin receptors bind tachykinins / positive regulation of uterine smooth muscle contraction / detection of abiotic stimulus / positive regulation of acute inflammatory response / operant conditioning / sperm ejaculation / positive regulation of lymphocyte proliferation / tachykinin receptor signaling pathway / response to ozone / phospholipase C-activating tachykinin receptor signaling pathway / sperm head / positive regulation of hormone secretion / positive regulation of action potential / response to auditory stimulus / positive regulation of synaptic transmission, GABAergic / positive regulation of blood pressure / smooth muscle contraction involved in micturition / positive regulation of leukocyte migration / regulation of smooth muscle cell proliferation / positive regulation of vascular permeability / regulation of smooth muscle cell migration / positive regulation of ossification / positive regulation of synaptic transmission, cholinergic / negative regulation of heart rate / eating behavior / behavioral response to pain / adenylate cyclase-activating G protein-coupled bile acid receptor signaling pathway / adenylate cyclase-activating serotonin receptor signaling pathway / response to progesterone / positive regulation of epithelial cell migration / angiotensin-mediated drinking behavior / regulation of skeletal muscle contraction / associative learning / developmental growth / hair follicle placode formation / response to electrical stimulus / PKA activation in glucagon signalling / renal water homeostasis / positive regulation of vasoconstriction / intracellular transport / positive regulation of stress fiber assembly / D1 dopamine receptor binding / long-term memory / sperm flagellum / neuronal dense core vesicle / vascular endothelial cell response to laminar fluid shear stress / Hedgehog 'off' state / activation of adenylate cyclase activity / adenylate cyclase-activating adrenergic receptor signaling pathway / cellular response to acidic pH / response to hormone / positive regulation of epithelial cell proliferation / cellular response to glucagon stimulus / intracellular glucose homeostasis / sperm midpiece / adenylate cyclase activator activity / positive regulation of insulin secretion involved in cellular response to glucose stimulus / trans-Golgi network membrane / response to nicotine / positive regulation of insulin secretion / negative regulation of inflammatory response to antigenic stimulus / neuropeptide signaling pathway / response to prostaglandin E / bone development / platelet aggregation / regulation of blood pressure / cognition / G-protein beta/gamma-subunit complex binding / Olfactory Signaling Pathway / phospholipase C-activating G protein-coupled receptor signaling pathway / Activation of the phototransduction cascade / G protein-coupled acetylcholine receptor signaling pathway / sensory perception of smell / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / G-protein activation / Glucagon signaling in metabolic regulation / G beta:gamma signalling through CDC42 / Prostacyclin signalling through prostacyclin receptor / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / response to estradiol / photoreceptor disc membrane / G beta:gamma signalling through BTK / ADP signalling through P2Y purinoceptor 12 / Sensory perception of sweet, bitter, and umami (glutamate) taste / Glucagon-type ligand receptors / Adrenaline,noradrenaline inhibits insulin secretion / cell-cell signaling / Vasopressin regulates renal water homeostasis via Aquaporins / G alpha (z) signalling events
Similarity search - Function
Tachykinin family / Tachykinin family / Neurokinin NK1 receptor / Tachykinin domain / Tachykinin family / Tachykinin/Neurokinin-like, conserved site / Tachykinin family signature. / Neurokinin receptor / G-protein alpha subunit, group S / G protein alpha subunit, helical insertion ...Tachykinin family / Tachykinin family / Neurokinin NK1 receptor / Tachykinin domain / Tachykinin family / Tachykinin/Neurokinin-like, conserved site / Tachykinin family signature. / Neurokinin receptor / G-protein alpha subunit, group S / G protein alpha subunit, helical insertion / G protein alpha subunit / Guanine nucleotide binding protein (G-protein), alpha subunit / G-protein alpha subunit / G-alpha domain profile. / G-protein, gamma subunit / G-protein gamma subunit domain profile. / G-protein gamma-like domain / G-protein gamma-like domain superfamily / GGL domain / G protein gamma subunit-like motifs / GGL domain / G protein beta WD-40 repeat protein / Guanine nucleotide-binding protein, beta subunit / G-protein, beta subunit / G-protein coupled receptors family 1 signature. / 7 transmembrane receptor (rhodopsin family) / G protein-coupled receptor, rhodopsin-like / GPCR, rhodopsin-like, 7TM / G-protein coupled receptors family 1 profile. / G-protein beta WD-40 repeat / WD40 repeat, conserved site / Trp-Asp (WD) repeats signature. / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Protachykinin-1 / Substance-P receptor / Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 / Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 / Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
Similarity search - Component
Biological speciesHomo sapiens (human)
Lama glama (llama)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.5 Å
AuthorsChen, M. / Pintilie, G.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Mental Health (NIH/NIMH)R21MH125285 United States
CitationJournal: Nat Methods / Year: 2024
Title: Improving resolution and resolvability of single-particle cryoEM structures using Gaussian mixture models.
Authors: Muyuan Chen / Michael F Schmid / Wah Chiu /
Abstract: Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian ...Cryogenic electron microscopy is widely used in structural biology, but its resolution is often limited by the dynamics of the macromolecule. Here we developed a refinement protocol based on Gaussian mixture models that integrates particle orientation and conformation estimation and improves the alignment for flexible domains of protein structures. We demonstrated this protocol on multiple datasets, resulting in improved resolution and resolvability, locally and globally, by visual and quantitative measures.
History
DepositionSep 5, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 15, 2023Provider: repository / Type: Initial release
Revision 1.1Nov 29, 2023Group: Database references / Category: citation / Item: _citation.pdbx_database_id_PubMed / _citation.title
Revision 1.2Jan 24, 2024Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.year
Revision 1.3Oct 9, 2024Group: Data collection / Structure summary
Category: em_admin / pdbx_entry_details / pdbx_modification_feature
Item: _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Guanine nucleotide-binding protein G(s) subunit alpha isoforms short
B: Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1
G: Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2
N: Nanobody 35
R: Substance-P receptor
S: Protachykinin-1


Theoretical massNumber of molelcules
Total (without water)141,5506
Polymers141,5506
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Guanine nucleotide-binding protein ... , 3 types, 3 molecules ABG

#1: Protein Guanine nucleotide-binding protein G(s) subunit alpha isoforms short / Adenylate cyclase-stimulating G alpha protein


Mass: 28907.684 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GNAS, GNAS1, GSP / Production host: Homo sapiens (human) / References: UniProt: P63092
#2: Protein Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 / Transducin beta chain 1


Mass: 40786.566 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GNB1 / Production host: Homo sapiens (human) / References: UniProt: P62873
#3: Protein Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 / G gamma-I


Mass: 7563.750 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GNG2 / Production host: Homo sapiens (human) / References: UniProt: P59768

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Antibody / Protein / Protein/peptide , 3 types, 3 molecules NRS

#4: Antibody Nanobody 35


Mass: 15398.067 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Lama glama (llama)
#5: Protein Substance-P receptor / SPR / NK-1 receptor / NK-1R / Tachykinin receptor 1


Mass: 47542.867 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TACR1, NK1R, TAC1R / Production host: Homo sapiens (human) / References: UniProt: P25103
#6: Protein/peptide Protachykinin-1 / PPT


Mass: 1350.629 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAC1, NKA, NKNA, TAC2 / Production host: Homo sapiens (human) / References: UniProt: P20366

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Details

Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Substance P bound to active human neurokinin 1 receptor in complex with miniGs399
Type: COMPLEX
Details: Reprocessing of EMPIAR-10786. Sample information is the same as EMD-24570, PDB-7RMH.
Entity ID: all / Source: NATURAL
Molecular weightValue: 0.141 MDa / Experimental value: YES
Source (natural)Organism: Homo sapiens (human)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 30 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

EM software
IDNameVersionCategory
12EMAN2.993D reconstruction
13PHENIX1.20.1model refinement
CTF correctionType: PHASE FLIPPING ONLY
3D reconstructionResolution: 2.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 288659 / Symmetry type: POINT
Atomic model buildingProtocol: FLEXIBLE FIT / Space: REAL
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0077611
ELECTRON MICROSCOPYf_angle_d1.02410381
ELECTRON MICROSCOPYf_dihedral_angle_d4.3281059
ELECTRON MICROSCOPYf_chiral_restr0.0441210
ELECTRON MICROSCOPYf_plane_restr0.0051295

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