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-Structure paper
タイトル | Euglena's atypical respiratory chain adapts to the discoidal cristae and flexible metabolism. |
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ジャーナル・号・ページ | Nat Commun, Vol. 15, Issue 1, Page 1628, Year 2024 |
掲載日 | 2024年2月22日 |
著者 | Zhaoxiang He / Mengchen Wu / Hongtao Tian / Liangdong Wang / Yiqi Hu / Fangzhu Han / Jiancang Zhou / Yong Wang / Long Zhou / |
PubMed 要旨 | Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron ...Euglena gracilis, a model organism of the eukaryotic supergroup Discoba harbouring also clinically important parasitic species, possesses diverse metabolic strategies and an atypical electron transport chain. While structures of the electron transport chain complexes and supercomplexes of most other eukaryotic clades have been reported, no similar structure is currently available for Discoba, limiting the understandings of its core metabolism and leaving a gap in the evolutionary tree of eukaryotic bioenergetics. Here, we report high-resolution cryo-EM structures of Euglena's respirasome I + III + IV and supercomplex III + IV. A previously unreported fatty acid synthesis domain locates on the tip of complex I's peripheral arm, providing a clear picture of its atypical subunit composition identified previously. Individual complexes are re-arranged in the respirasome to adapt to the non-uniform membrane curvature of the discoidal cristae. Furthermore, Euglena's conformationally rigid complex I is deactivated by restricting ubiquinone's access to its substrate tunnel. Our findings provide structural insights for therapeutic developments against euglenozoan parasite infections. |
リンク | Nat Commun / PubMed:38388527 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.69 - 3.14 Å |
構造データ | EMDB-35662: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I peripheral arm focused EMDB-35663: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I proximal membrane arm focused EMDB-35664: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex I distal membrane arm focused EMDB-35665: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex III2 focused EMDB-35666: Cryo-EM map of Euglena gracilis respirasome I+III2+IV, complex IV focused EMDB-35667: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex III2 focused EMDB-35668: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex IV focused EMDB-35669: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, complex IV focused EMDB-35720, PDB-8iuf: EMDB-35723: Cryo-EM map of Euglena gracilis supercomplex III2+IV2, composite EMDB-35819: Cryo-EM map of Euglena gracilis respirasome I+III2+IV EMDB-35820: Cryo-EM map of Euglena gracilis supercomplex III2+IV2 EMDB-36094: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, peripheral arm focused EMDB-36096: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, proximal membrane arm focused EMDB-36097: Cryo-EM map of Euglena gracilis respiratory complex I in the deactive state, distal membrane arm focused EMDB-36098: Cryo-EM consensus map of Euglena gracilis respiratory complex I, deactive state EMDB-36099: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, peripheral arm focused EMDB-36100: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, proximal membrane arm focused EMDB-36101: Cryo-EM map of Euglena gracilis respiratory complex I in the turnover state, distal membrane arm focused EMDB-36102: Cryo-EM consensus map of Euglena gracilis respiratory complex I, turnover state EMDB-36103: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, peripheral arm focused EMDB-36104: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, proximal membrane arm focused EMDB-36105: Cryo-EM map of Euglena gracilis respiratory complex I in the NADH state, distal membrane focused EMDB-36106: Cryo-EM consensus map of Euglena gracilis respiratory complex I, NADH state EMDB-36107: Cryo-EM composite map of Euglena gracilis respiratory complex I, deactive state EMDB-36108: Cryo-EM composite map of Euglena gracilis complex I, turnover state EMDB-36109: Cryo-EM composite map of Euglena gracilis complex I, NADH state |
化合物 | ChemComp-FES: ChemComp-SF4: ChemComp-K: ChemComp-PC1: ChemComp-3PE: ChemComp-S12: ChemComp-CDL: ChemComp-ZN: ChemComp-NDP: ChemComp-ZMP: ChemComp-HEA: ChemComp-CU: ChemComp-MG: ChemComp-PX2: ChemComp-HEM: ChemComp-HEC: ChemComp-FMN: ChemComp-NAI: |
由来 |
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キーワード | ELECTRON TRANSPORT / Electron transport chain / supercomplex / membrane protein / Euglena gracilis / complex / respiratory complex |