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-Structure paper
タイトル | Structure of mammalian eIF3 in the context of the 43S preinitiation complex. |
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ジャーナル・号・ページ | Nature, Vol. 525, Issue 7570, Page 491-495, Year 2015 |
掲載日 | 2015年9月24日 |
![]() | Amedee des Georges / Vidya Dhote / Lauriane Kuhn / Christopher U T Hellen / Tatyana V Pestova / Joachim Frank / Yaser Hashem / ![]() ![]() |
PubMed 要旨 | During eukaryotic translation initiation, 43S complexes, comprising a 40S ribosomal subunit, initiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of ...During eukaryotic translation initiation, 43S complexes, comprising a 40S ribosomal subunit, initiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of messenger RNA and scan along it to the initiation codon. Scanning on structured mRNAs also requires the DExH-box protein DHX29. Mammalian eIF3 contains 13 subunits and participates in nearly all steps of translation initiation. Eight subunits having PCI (proteasome, COP9 signalosome, eIF3) or MPN (Mpr1, Pad1, amino-terminal) domains constitute the structural core of eIF3, to which five peripheral subunits are flexibly linked. Here we present a cryo-electron microscopy structure of eIF3 in the context of the DHX29-bound 43S complex, showing the PCI/MPN core at ∼6 Å resolution. It reveals the organization of the individual subunits and their interactions with components of the 43S complex. We were able to build near-complete polyalanine-level models of the eIF3 PCI/MPN core and of two peripheral subunits. The implications for understanding mRNA ribosomal attachment and scanning are discussed. |
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手法 | EM (単粒子) |
解像度 | 6.0 - 9.0 Å |
構造データ | EMDB-3056, PDB-5a5t: EMDB-3057, PDB-5a5u: ![]() EMDB-3058: |
由来 |
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![]() | HYDROLASE / EIF3 / EUKARYOTIC INITIATION FACTOR 3 / PREINITIATION COMPLEX / PCI/MPN CORE / EIF3G/I/B / EIF3D / TRANSLATION / EIF3 OCTAMER CORE / MAMMALIAN PREINITIATION 34S COMPLEX |