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TitleStructure of mammalian eIF3 in the context of the 43S preinitiation complex.
Journal, issue, pagesNature, Vol. 525, Issue 7570, Page 491-495, Year 2015
Publish dateSep 24, 2015
AuthorsAmedee des Georges / Vidya Dhote / Lauriane Kuhn / Christopher U T Hellen / Tatyana V Pestova / Joachim Frank / Yaser Hashem /
PubMed AbstractDuring eukaryotic translation initiation, 43S complexes, comprising a 40S ribosomal subunit, initiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of ...During eukaryotic translation initiation, 43S complexes, comprising a 40S ribosomal subunit, initiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of messenger RNA and scan along it to the initiation codon. Scanning on structured mRNAs also requires the DExH-box protein DHX29. Mammalian eIF3 contains 13 subunits and participates in nearly all steps of translation initiation. Eight subunits having PCI (proteasome, COP9 signalosome, eIF3) or MPN (Mpr1, Pad1, amino-terminal) domains constitute the structural core of eIF3, to which five peripheral subunits are flexibly linked. Here we present a cryo-electron microscopy structure of eIF3 in the context of the DHX29-bound 43S complex, showing the PCI/MPN core at ∼6 Å resolution. It reveals the organization of the individual subunits and their interactions with components of the 43S complex. We were able to build near-complete polyalanine-level models of the eIF3 PCI/MPN core and of two peripheral subunits. The implications for understanding mRNA ribosomal attachment and scanning are discussed.
External linksNature / PubMed:26344199 / PubMed Central
MethodsEM (single particle)
Resolution6.0 - 9.0 Å
Structure data

3056

5a5t

EMDB-3056, PDB-5a5t:
Structure of mammalian eIF3 in the context of the 43S preinitiation complex
Method: EM (single particle) / Resolution: 6.0 Å

3057

5a5u

EMDB-3057, PDB-5a5u:
Structure of mammalian eIF3 in the context of the 43S preinitiation complex
Method: EM (single particle) / Resolution: 9.0 Å

EMDB-3058:
Structure of mammalian eIF3 in the context of the 43S preinitiation complex
Method: EM (single particle) / Resolution: 9.0 Å

Source
  • oryctolagus cuniculus (rabbit)
KeywordsHYDROLASE / EIF3 / EUKARYOTIC INITIATION FACTOR 3 / PREINITIATION COMPLEX / PCI/MPN CORE / EIF3G/I/B / EIF3D / TRANSLATION / EIF3 OCTAMER CORE / MAMMALIAN PREINITIATION 34S COMPLEX

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