EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for assembly and lipid-mediated gating of LRRC8A:C volume-regulated anion channels.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 30, Issue 6, Page 841-852, Year 2023
掲載日	2023年3月16日
著者	David M Kern / Julia Bleier / Somnath Mukherjee / Jennifer M Hill / Anthony A Kossiakoff / Ehud Y Isacoff / Stephen G Brohawn /
PubMed 要旨	Leucine-rich repeat-containing protein 8 (LRRC8) family members form volume-regulated anion channels activated by hypoosmotic cell swelling. LRRC8 channels are ubiquitously expressed in vertebrate ...Leucine-rich repeat-containing protein 8 (LRRC8) family members form volume-regulated anion channels activated by hypoosmotic cell swelling. LRRC8 channels are ubiquitously expressed in vertebrate cells as heteromeric assemblies of LRRC8A (SWELL1) and LRRC8B-E subunits. Channels of different subunit composition have distinct properties that explain the functional diversity of LRRC8 currents across cell types. However, the basis for heteromeric LRRC8 channel assembly and function is unknown. Here we leverage a fiducial-tagging strategy to determine single-particle cryo-EM structures of heterohexameric LRRC8A:C channels in multiple conformations. Compared to homomers, LRRC8A:C channels show pronounced differences in architecture due to heterotypic LRR interactions that displace subunits away from the conduction axis and poise the channel for activation. Structures and functional studies further reveal that lipids embedded in the channel pore block ion conduction in the closed state. These results provide insight into determinants for heteromeric LRRC8 channel assembly, activity and gating by lipids.
リンク	Nat Struct Mol Biol / PubMed:36928458
手法	EM (単粒子)
解像度	2.95 - 4.32 Å
構造データ	27674 8dr8 EMDB-27674, PDB-8dr8: LRRC8A:C conformation 2 (oblong) top mask 手法: EM (単粒子) / 解像度: 3.04 Å 27675 8dra EMDB-27675, PDB-8dra: LRRC8A:C conformation 2 (oblong) LRR mask 手法: EM (単粒子) / 解像度: 3.98 Å 27676 8dre EMDB-27676, PDB-8dre: LRRC8A:C conformation 2 (oblong) 手法: EM (単粒子) / 解像度: 3.18 Å 27677 8drk EMDB-27677, PDB-8drk: LRRC8A:C conformation 1 (round) top focus 手法: EM (単粒子) / 解像度: 2.95 Å 27678 8drn EMDB-27678, PDB-8drn: LRRC8A:C conformation 1 (round) LRR focus 1 手法: EM (単粒子) / 解像度: 4.12 Å 27679 8dro EMDB-27679, PDB-8dro: LRRC8A:C conformation 1 (round) LRR focus 2 手法: EM (単粒子) / 解像度: 4.06 Å 27681 8drq EMDB-27681, PDB-8drq: LRRC8A:C conformation 1 (round) LRR focus 3 手法: EM (単粒子) / 解像度: 4.16 Å 27682 8ds3 EMDB-27682, PDB-8ds3: LRRC8A:C conformation 1 (round) 手法: EM (単粒子) / 解像度: 3.07 Å 27686 8ds9 EMDB-27686, PDB-8ds9: LRRC8A:C in MSPE3D1 nanodisc top focus 手法: EM (単粒子) / 解像度: 3.17 Å 27687 8dsa EMDB-27687, PDB-8dsa: LRRC8A:C in MSP1E3D1 nanodisc 手法: EM (単粒子) / 解像度: 3.48 Å 28894 8f74 EMDB-28894, PDB-8f74: LRRC8A(T48D):C conformation 2 top focus 手法: EM (単粒子) / 解像度: 3.1 Å 28895 8f75 EMDB-28895, PDB-8f75: LRRC8A(T48D):C conformation 2 LRR focus 手法: EM (単粒子) / 解像度: 4.0 Å 28897 8f77 EMDB-28897, PDB-8f77: LRRC8A(T48D):C conformation 2 top focus 手法: EM (単粒子) / 解像度: 3.15 Å 28898 8f79 EMDB-28898, PDB-8f79: LRRC8A(T48D):C conformation 2 top focus 手法: EM (単粒子) / 解像度: 3.15 Å 28901 8f7b EMDB-28901, PDB-8f7b: LRRC8A(T48D):C conformation 2 top focus 手法: EM (単粒子) / 解像度: 3.15 Å 28903 8f7d EMDB-28903, PDB-8f7d: LRRC8A(T48D):C conformation 2 top focus 手法: EM (単粒子) / 解像度: 4.2 Å 28904 8f7e EMDB-28904, PDB-8f7e: LRRC8A(T48D):C conformation 2 top focus 手法: EM (単粒子) / 解像度: 4.13 Å 28905 8f7j EMDB-28905, PDB-8f7j: LRRC8A(T48D):C conformation 2 top focus 手法: EM (単粒子) / 解像度: 4.32 Å
化合物	ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質YM ChemComp-HOH*: WATER
由来	mus musculus (ハツカネズミ)
キーワード	MEMBRANE PROTEIN / ION CHANNEL / VOLUME-REGULATION