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-Structure paper
タイトル | A cryo-electron microscopic approach to elucidate protein structures from human brain microsomes. |
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ジャーナル・号・ページ | Life Sci Alliance, Vol. 6, Issue 2, Year 2023 |
掲載日 | 2022年11月30日 |
著者 | Marios L Tringides / Zhemin Zhang / Christopher E Morgan / Chih-Chia Su / Edward W Yu / |
PubMed 要旨 | We recently developed a "Build and Retrieve" cryo-electron microscopy (cryo-EM) methodology, which is capable of simultaneously producing near-atomic resolution cryo-EM maps for several individual ...We recently developed a "Build and Retrieve" cryo-electron microscopy (cryo-EM) methodology, which is capable of simultaneously producing near-atomic resolution cryo-EM maps for several individual proteins from a heterogeneous, multiprotein sample. Here we report the use of "Build and Retrieve" to define the composition of a raw human brain microsomal lysate. From this sample, we simultaneously identify and solve cryo-EM structures of five different brain enzymes whose functions affect neurotransmitter recycling, iron metabolism, glycolysis, axonal development, energy homeostasis, and retinoic acid biosynthesis. Interestingly, malfunction of these important proteins has been directly linked to several neurodegenerative disorders, such as Alzheimer's, Huntington's, and Parkinson's diseases. Our work underscores the importance of cryo-EM in facilitating tissue and organ proteomics at the atomic level. |
リンク | Life Sci Alliance / PubMed:36450447 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.69 - 3.4 Å |
構造データ | EMDB-27574, PDB-8dnm: EMDB-27575, PDB-8dno: EMDB-27576, PDB-8dnp: EMDB-27579, PDB-8dns: EMDB-27580, PDB-8dnu: |
化合物 | ChemComp-FE: ChemComp-MN: |
由来 |
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キーワード | HYDROLASE / human brain / DPYSL2 / OXIDOREDUCTASE / ALDH1A1 / METAL BINDING PROTEIN / ferritin / heavy chain / GAPDH / LIGASE / Glutamine Synthetase |