EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular architecture of 40S translation initiation complexes on the hepatitis C virus IRES.
ジャーナル・号・ページ	EMBO J, Vol. 41, Issue 16, Page e110581, Year 2022
掲載日	2022年8月16日
著者	Zuben P Brown / Irina S Abaeva / Swastik De / Christopher U T Hellen / Tatyana V Pestova / Joachim Frank /
PubMed 要旨	Hepatitis C virus mRNA contains an internal ribosome entry site (IRES) that mediates end-independent translation initiation, requiring a subset of eukaryotic initiation factors (eIFs). Biochemical ...Hepatitis C virus mRNA contains an internal ribosome entry site (IRES) that mediates end-independent translation initiation, requiring a subset of eukaryotic initiation factors (eIFs). Biochemical studies revealed that direct binding of the IRES to the 40S ribosomal subunit places the initiation codon into the P site, where it base pairs with eIF2-bound Met-tRNAiMet forming a 48S initiation complex. Subsequently, eIF5 and eIF5B mediate subunit joining, yielding an elongation-competent 80S ribosome. Initiation can also proceed without eIF2, in which case Met-tRNAiMet is recruited directly by eIF5B. However, the structures of initiation complexes assembled on the HCV IRES, the transitions between different states, and the accompanying conformational changes have remained unknown. To fill these gaps, we now obtained cryo-EM structures of IRES initiation complexes, at resolutions up to 3.5 Å, that cover all major stages from the initial ribosomal association, through eIF2-containing 48S initiation complexes, to eIF5B-containing complexes immediately prior to subunit joining. These structures provide insights into the dynamic network of 40S/IRES contacts, highlight the role of IRES domain II, and reveal conformational changes that occur during the transition from eIF2- to eIF5B-containing 48S complexes and prepare them for subunit joining.
リンク	EMBO J / PubMed:35822879 / PubMed Central
手法	EM (単粒子)
解像度	3.1 - 6.2 Å
構造データ	25529 7syi EMDB-25529, PDB-7syi: Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 3(delta dII) 手法: EM (単粒子) / 解像度: 4.5 Å 25530 7syj EMDB-25530, PDB-7syj: Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 4(delta dII) 手法: EM (単粒子) / 解像度: 4.8 Å 25531 7syk EMDB-25531, PDB-7syk: Structure of the HCV IRES binding to the 40S ribosomal subunit, closed conformation. Structure 5(delta dII) 手法: EM (単粒子) / 解像度: 4.2 Å 25532 7syl EMDB-25532, PDB-7syl: Structure of the HCV IRES bound to the 40S ribosomal subunit, closed conformation. Structure 6(delta dII) 手法: EM (単粒子) / 解像度: 4.5 Å 25535 7syo EMDB-25535, PDB-7syo: Structure of the HCV IRES bound to the 40S ribosomal subunit, head open. Structure 9(delta dII) 手法: EM (単粒子) / 解像度: 4.6 Å 25536 7syp EMDB-25536, PDB-7syp: Structure of the wt IRES and 40S ribosome binary complex, open conformation. Structure 10(wt) 手法: EM (単粒子) / 解像度: 4.0 Å 25537 7syq EMDB-25537, PDB-7syq: Structure of the wt IRES and 40S ribosome ternary complex, open conformation. Structure 11(wt) 手法: EM (単粒子) / 解像度: 3.8 Å 25538 7syr EMDB-25538, PDB-7syr: Structure of the wt IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(wt). 手法: EM (単粒子) / 解像度: 3.6 Å 25539 7sys EMDB-25539, PDB-7sys: Structure of the delta dII IRES eIF2-containing 48S initiation complex, closed conformation. Structure 12(delta dII). 手法: EM (単粒子) / 解像度: 3.5 Å 25540 7syt EMDB-25540, PDB-7syt: Structure of the wt IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(wt) 手法: EM (単粒子) / 解像度: 4.4 Å 25541 7syu EMDB-25541, PDB-7syu: Structure of the delta dII IRES w/o eIF2 48S initiation complex, closed conformation. Structure 13(delta dII) 手法: EM (単粒子) / 解像度: 4.6 Å 25542 7syv EMDB-25542, PDB-7syv: Structure of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation. Structure 14(wt) 手法: EM (単粒子) / 解像度: 3.9 Å 25543 7syw EMDB-25543, PDB-7syw: Structure of the wt IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(wt) 手法: EM (単粒子) / 解像度: 3.7 Å 25544 7syx EMDB-25544, PDB-7syx: Structure of the delta dII IRES eIF5B-containing 48S initiation complex, closed conformation. Structure 15(delta dII) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-25545: Map of wt IRES eIF2-containing 48S initiation complex with IRES domain II in position 1. Structure 16(wt). 手法: EM (単粒子) / 解像度: 3.76 Å EMDB-25546: Map of wt IRES eIF2-containing 48S initiation complex with IRES domain II in position 2. Structure 17(wt). 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-25547: Map of wt IRES eIF2-containing 48S initiation complex with IRES domain II in position 3. Structure 18(wt) 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-25548: Map of wt IRES eIF2-containing 48S initiation complex with IRES domain II in position 4. Structure 19(wt) 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-25549: Map of wt IRES eIF2-containing 48S initiation complex with IRES domain II in position 5. Structure 20(wt) 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-25550: Map of wt IRES eIF2-containing 48S initiation complex with IRES domain II in position 6. Structure 21(wt) 手法: EM (単粒子) / 解像度: 3.76 Å EMDB-25551: Map of wt IRES eIF5B-containing 48S initiation complex with IRES domain II in position 7. Structure 22(wt) 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-25552: Map of wt IRES eIF5B-containing 48S initiation complex with IRES domain II in position 8. Structure 23(wt) 手法: EM (単粒子) / 解像度: 5.4 Å EMDB-25553: Map of wt IRES eIF5B-containing 48S initiation complex with IRES domain II in position 9. Structure 24(wt) 手法: EM (単粒子) / 解像度: 5.2 Å EMDB-25554: Map of wt IRES eIF5B-containing 48S initiation complex with IRES domain II in position 10. Structure 25(wt) 手法: EM (単粒子) / 解像度: 4.9 Å EMDB-25555: Map of wt IRES eIF5B-containing 48S initiation complex with IRES domain II in position 11. Structure 26(wt) 手法: EM (単粒子) / 解像度: 6.0 Å EMDB-25556: Map of wt IRES eIF5B-containing 48S initiation complex with IRES domain II in position 12. Structure 27(wt) 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-25557: Map of wt IRES eIF5B-containing 48S initiation complex with IRES domain II in position 13. Structure 28(wt) 手法: EM (単粒子) / 解像度: 6.2 Å EMDB-25588: Consensus map of open 40S ribosome from wt IRES eIF2-containing sample 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-25589: Consensus map of open 40S ribosome from wt IRES eIF5B-containing sample 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-25594: Consensus map of open 40S ribosome from wt IRES eIF5B-, and eIF2-containing sample 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-25595: Consensus map of closed 40S ribosome from wt IRES eIF2-containing sample 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-25596: Consensus map of closed 40S ribosome from delta dII IRES eIF2-containing sample 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-25597: Consensus map of open 40S ribosome from wt IRES eIF5B-containing sample 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-25598: Consensus map of closed 40S ribosome from wt IRES eIF5B-containing sample 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-25599: Consensus map of closed 40S ribosome from delta dII IRES eIF5B-containing sample 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-25602: Map of the wt IRES eIF5B-containing 48S initiation complex, closed conformation 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-25603: Map of the wt IRES eIF5B-containing pre-48S initiation complex, open conformation 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-25604: Map of the wt IRES eIF2-containing 48S initiation complex, closed conformation 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-25620: Map of the delta IRES eIF2-containing 48S initiation complex w/o eIF2, closed conformation 手法: EM (単粒子) / 解像度: 3.55 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-NA*: Unknown entry / ナトリウムカチオン
由来	oryctolagus cuniculus (ウサギ) hepacivirus c (ウイルス) hepatitis c virus genotype 1a (C型肝炎ウイルス) hepatitis c virus (isolate 1) (C型肝炎ウイルス) Rabbit (ウサギ) homo sapiens (ヒト)
キーワード	RIBOSOME / HCV / IRES / 40S