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-Structure paper
タイトル | High-resolution cryo-EM structure of the virus Sf6 genome delivery tail machine. |
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ジャーナル・号・ページ | Sci Adv, Vol. 8, Issue 49, Page eadc9641, Year 2022 |
掲載日 | 2022年12月9日 |
著者 | Fenglin Li / Chun-Feng David Hou / Ruoyu Yang / Richard Whitehead / Carolyn M Teschke / Gino Cingolani / |
PubMed 要旨 | Sf6 is a bacterial virus that infects the human pathogen Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom ...Sf6 is a bacterial virus that infects the human pathogen Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom resolution. We built de novo structures of all tail components and resolved four symmetry-mismatched interfaces. Unexpectedly, we found that the tail exists in two conformations, rotated by ~6° with respect to the capsid. The two tail conformers are identical in structure but differ solely in how the portal and head-to-tail adaptor carboxyl termini bond with the capsid at the fivefold vertex, similar to a diamond held over a five-pronged ring in two nonidentical states. Thus, in the mature Sf6 tail, the portal structure does not morph locally to accommodate the symmetry mismatch but exists in two energetic minima rotated by a discrete angle. We propose that the design principles of the Sf6 tail are conserved across P22-like Podoviridae. |
リンク | Sci Adv / PubMed:36475795 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.76 - 3.73 Å |
構造データ | EMDB-25101, PDB-7sfs: EMDB-25106, PDB-7sg7: EMDB-25365, PDB-7sp4: EMDB-25372, PDB-7spu: EMDB-26582, PDB-7ukj: |
由来 |
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キーワード | STRUCTURAL PROTEIN / in situ / phage / portal / gp7 / gp3 / gp8 / gp14 / bacteriophage / Sf6 portal / coat protein / symmetry mismatch / VIRAL PROTEIN |