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Yorodumi- PDB-7sg7: In situ cryo-EM structure of bacteriophage Sf6 gp8:gp14N complex ... -
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-Basic information
Entry | Database: PDB / ID: 7sg7 | ||||||
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Title | In situ cryo-EM structure of bacteriophage Sf6 gp8:gp14N complex at 2.8 A resolution | ||||||
Components |
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Keywords | STRUCTURAL PROTEIN / in situ / phage / gp8 / gp14 | ||||||
Function / homology | Function and homology information virus tail / symbiont entry into host cell / virion attachment to host cell Similarity search - Function | ||||||
Biological species | Shigella phage Sf6 (virus) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.83 Å | ||||||
Authors | Li, F. / Cingolani, G. / Hou, C. | ||||||
Funding support | United States, 1items
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Citation | Journal: Sci Adv / Year: 2022 Title: High-resolution cryo-EM structure of the virus Sf6 genome delivery tail machine. Authors: Fenglin Li / Chun-Feng David Hou / Ruoyu Yang / Richard Whitehead / Carolyn M Teschke / Gino Cingolani / Abstract: Sf6 is a bacterial virus that infects the human pathogen Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom ...Sf6 is a bacterial virus that infects the human pathogen Here, we describe the cryo-electron microscopy structure of the Sf6 tail machine before DNA ejection, which we determined at a 2.7-angstrom resolution. We built de novo structures of all tail components and resolved four symmetry-mismatched interfaces. Unexpectedly, we found that the tail exists in two conformations, rotated by ~6° with respect to the capsid. The two tail conformers are identical in structure but differ solely in how the portal and head-to-tail adaptor carboxyl termini bond with the capsid at the fivefold vertex, similar to a diamond held over a five-pronged ring in two nonidentical states. Thus, in the mature Sf6 tail, the portal structure does not morph locally to accommodate the symmetry mismatch but exists in two energetic minima rotated by a discrete angle. We propose that the design principles of the Sf6 tail are conserved across P22-like Podoviridae. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7sg7.cif.gz | 957.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7sg7.ent.gz | 726.3 KB | Display | PDB format |
PDBx/mmJSON format | 7sg7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7sg7_validation.pdf.gz | 1.1 MB | Display | wwPDB validaton report |
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Full document | 7sg7_full_validation.pdf.gz | 1.1 MB | Display | |
Data in XML | 7sg7_validation.xml.gz | 122.4 KB | Display | |
Data in CIF | 7sg7_validation.cif.gz | 194.2 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sg/7sg7 ftp://data.pdbj.org/pub/pdb/validation_reports/sg/7sg7 | HTTPS FTP |
-Related structure data
Related structure data | 25106MC 7sfsC 7sp4C 7spuC 7ukjC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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Noncrystallographic symmetry (NCS) | NCS domain:
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