EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Three-step mechanism of promoter escape by RNA polymerase II.
ジャーナル・号・ページ	Mol Cell, Vol. 84, Issue 9, Page 1699-11710.e6, Year 2024
掲載日	2024年5月2日
著者	Yumeng Zhan / Frauke Grabbe / Elisa Oberbeckmann / Christian Dienemann / Patrick Cramer /
PubMed 要旨	The transition from transcription initiation to elongation is highly regulated in human cells but remains incompletely understood at the structural level. In particular, it is unclear how ...The transition from transcription initiation to elongation is highly regulated in human cells but remains incompletely understood at the structural level. In particular, it is unclear how interactions between RNA polymerase II (RNA Pol II) and initiation factors are broken to enable promoter escape. Here, we reconstitute RNA Pol II promoter escape in vitro and determine high-resolution structures of initially transcribing complexes containing 8-, 10-, and 12-nt ordered RNAs and two elongation complexes containing 14-nt RNAs. We suggest that promoter escape occurs in three major steps. First, the growing RNA displaces the B-reader element of the initiation factor TFIIB without evicting TFIIB. Second, the rewinding of the transcription bubble coincides with the eviction of TFIIA, TFIIB, and TBP. Third, the binding of DSIF and NELF facilitates TFIIE and TFIIH dissociation, establishing the paused elongation complex. This three-step model for promoter escape fills a gap in our understanding of the initiation-elongation transition of RNA Pol II transcription.
リンク	Mol Cell / PubMed:38604172
手法	EM (単粒子)
解像度	2.9 - 7.4 Å
構造データ	19718 8s51 EMDB-19718, PDB-8s51: RNA polymerase II core initially transcribing complex with an ordered RNA of 8 nt 手法: EM (単粒子) / 解像度: 3.1 Å 19719 8s52 EMDB-19719, PDB-8s52: RNA polymerase II core initially transcribing complex with an ordered RNA of 10 nt 手法: EM (単粒子) / 解像度: 2.9 Å 19720 8s54 EMDB-19720, PDB-8s54: RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state b (composite structure) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-19721: RNA polymerase II initially transcribing complex with an ordered RNA of 8 nt 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-19722: RNA polymerase II initially transcribing complex with an ordered RNA of 10 nt 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-19723: RNA polymerase II initially transcribing complex with an ordered RNA of 12 nt 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-19724: RNA polymerase II early elongation complex bound to TFIIE, TFIIF and TFIIH - state a 手法: EM (単粒子) / 解像度: 6.0 Å EMDB-19725: RNA polymerase II early elongation complex bound to TFIIE, TFIIF and TFIIH - state b 手法: EM (単粒子) / 解像度: 6.2 Å 19726 8s55 EMDB-19726, PDB-8s55: RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state a (composite structure) 手法: EM (単粒子) / 解像度: 3.4 Å 19743 8s5n EMDB-19743, PDB-8s5n: RNA polymerase II core initially transcribing complex with an ordered RNA of 12 nt 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-19795: RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state a (global map) 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-19796: RNA polymerase II early elongation complex bound to TFIIE and TFIIF - state b (global map) 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-19797: EC14 core Pol II focused map 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	sus scrofa (ブタ) unidentified adenovirus (ウイルス) homo sapiens (ヒト)
キーワード	TRANSCRIPTION (転写 (生物学)) / RNA polymerase II (RNAポリメラーゼII) / promoter escape / de novo initiation / de novo transcription