EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of transcription reduction by a promoter-proximal +1 nucleosome.
ジャーナル・号・ページ	Mol Cell, Vol. 83, Issue 11, Page 1798-11809.e7, Year 2023
掲載日	2023年6月1日
著者	Julio Abril-Garrido / Christian Dienemann / Frauke Grabbe / Taras Velychko / Michael Lidschreiber / Haibo Wang / Patrick Cramer /
PubMed 要旨	At active human genes, the +1 nucleosome is located downstream of the RNA polymerase II (RNA Pol II) pre-initiation complex (PIC). However, at inactive genes, the +1 nucleosome is found further ...At active human genes, the +1 nucleosome is located downstream of the RNA polymerase II (RNA Pol II) pre-initiation complex (PIC). However, at inactive genes, the +1 nucleosome is found further upstream, at a promoter-proximal location. Here, we establish a model system to show that a promoter-proximal +1 nucleosome can reduce RNA synthesis in vivo and in vitro, and we analyze its structural basis. We find that the PIC assembles normally when the edge of the +1 nucleosome is located 18 base pairs (bp) downstream of the transcription start site (TSS). However, when the nucleosome edge is located further upstream, only 10 bp downstream of the TSS, the PIC adopts an inhibited state. The transcription factor IIH (TFIIH) shows a closed conformation and its subunit XPB contacts DNA with only one of its two ATPase lobes, inconsistent with DNA opening. These results provide a mechanism for nucleosome-dependent regulation of transcription initiation.
リンク	Mol Cell / PubMed:37148879 / PubMed Central
手法	EM (単粒子)
解像度	3.0 - 4.5 Å
構造データ	16274 8bvw EMDB-16274, PDB-8bvw: RNA polymerase II pre-initiation complex with the distal +1 nucleosome (PIC-Nuc18W) 手法: EM (単粒子) / 解像度: 4.0 Å 16331 8byq EMDB-16331, PDB-8byq: RNA polymerase II pre-initiation complex with the proximal +1 nucleosome (PIC-Nuc10W) 手法: EM (単粒子) / 解像度: 4.1 Å 16335 8bz1 EMDB-16335, PDB-8bz1: RNA polymerase II core pre-initiation complex with the proximal +1 nucleosome (cPIC-Nuc10W) 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-16336: RNA polymerase II core pre-initiation complex focused map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-16337: Proximal +1 nucleosome focused map 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-16338: RNA polymerase II core pre-initiation complex with the proximal +1 nucleosome composite map 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-16339: RNA polymerase II core pre-initiation complex focused map 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-16340: Proximal +1 nucleosome focused map 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-16341: TFIIH subcomplex CDK-activating kinase module focused map 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-16342: General transcription factor IIH in closed conformation focused map 手法: EM (単粒子) / 解像度: 4.5 Å EMDB-16343: RNA polymerase II pre-initiation complex with the proximal +1 nucleosome composite map 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-16365: RNA polymerase II core pre-initiation complex focused map 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-16366: Distal +1 nucleosome focused map 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-16367: General transcription factor IIH in open conformation focused map 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-16368: TFIIH subcomplex CDK-activating kinase module focused map 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-16369: RNA polymerase II pre-initiation complex with the distal +1 nucleosome composite map 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	homo sapiens (ヒト) sus scrofa (ブタ) xenopus laevis (アフリカツメガエル) unidentified adenovirus (ウイルス) pig (ブタ)
キーワード	TRANSCRIPTION (転写 (生物学)) / Mammalian PIC / +1 nucleosome / transcription initiation (転写 (生物学))