EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for clearing of ribosome collisions by the RQT complex.
ジャーナル・号・ページ	Nat Commun, Vol. 14, Issue 1, Page 921, Year 2023
掲載日	2023年2月17日
著者	Katharina Best / Ken Ikeuchi / Lukas Kater / Daniel Best / Joanna Musial / Yoshitaka Matsuo / Otto Berninghausen / Thomas Becker / Toshifumi Inada / Roland Beckmann /
PubMed 要旨	Translation of aberrant messenger RNAs can cause stalling of ribosomes resulting in ribosomal collisions. Collided ribosomes are specifically recognized to initiate stress responses and quality ...Translation of aberrant messenger RNAs can cause stalling of ribosomes resulting in ribosomal collisions. Collided ribosomes are specifically recognized to initiate stress responses and quality control pathways. Ribosome-associated quality control facilitates the degradation of incomplete translation products and requires dissociation of the stalled ribosomes. A central event is therefore the splitting of collided ribosomes by the ribosome quality control trigger complex, RQT, by an unknown mechanism. Here we show that RQT requires accessible mRNA and the presence of a neighboring ribosome. Cryogenic electron microscopy of RQT-ribosome complexes reveals that RQT engages the 40S subunit of the lead ribosome and can switch between two conformations. We propose that the Ski2-like helicase 1 (Slh1) subunit of RQT applies a pulling force on the mRNA, causing destabilizing conformational changes of the small ribosomal subunit, ultimately resulting in subunit dissociation. Our findings provide conceptual framework for a helicase-driven ribosomal splitting mechanism.
リンク	Nat Commun / PubMed:36801861 / PubMed Central
手法	EM (単粒子)
解像度	2.4 - 4.8 Å
構造データ	14861 7zpq EMDB-14861, PDB-7zpq: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C1) 手法: EM (単粒子) / 解像度: 3.47 Å 14921 7zrs EMDB-14921, PDB-7zrs: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - composite map 手法: EM (単粒子) / 解像度: 4.8 Å 14926 7zs5 EMDB-14926, PDB-7zs5: Structure of 60S ribosomal subunit from S. cerevisiae with eIF6 and tRNA 手法: EM (単粒子) / 解像度: 3.2 Å 14978 7zuw EMDB-14978, PDB-7zuw: Structure of RQT (C1) bound to the stalled ribosome in a disome unit from S. cerevisiae 手法: EM (単粒子) / 解像度: 4.3 Å 14979 7zux EMDB-14979, PDB-7zux: Collided ribosome in a disome unit from S. cerevisiae 手法: EM (単粒子) / 解像度: 2.5 Å EMDB-15228: RQT-bound 80S ribosome from S. cerevisiae (C1, raw consensus map) 手法: EM (単粒子) / 解像度: 2.4 Å EMDB-16550: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - focused Refinement 80S 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-16551: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - focused Refinement RQT 手法: EM (単粒子) / 解像度: 4.8 Å EMDB-16552: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - composite map 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-16553: Structure of RQT (C1) bound to the stalled ribosome in a disome unit from S. cerevisiae - focused Refinement 80S 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-16554: Structure of RQT (C1) bound to the stalled ribosome in a disome unit from S. cerevisiae - focused Refinement RQT 手法: EM (単粒子) / 解像度: 4.3 Å EMDB-16571: Structure of the RQT-bound 80S ribosome from S. cerevisiae (C2) - consensus map 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-UNK: UNKNOWN / (S)-2-アミノ酪酸
由来	saccharomyces cerevisiae (パン酵母)
キーワード	RIBOSOME / collision / RNA binding / RQT / RQC / splitting / eIF6 / Tif6p