EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	mRNA recognition and packaging by the human transcription-export complex.
ジャーナル・号・ページ	Nature, Vol. 616, Issue 7958, Page 828-835, Year 2023
掲載日	2023年4月5日
著者	Belén Pacheco-Fiallos / Matthias K Vorländer / Daria Riabov-Bassat / Laura Fin / Francis J O'Reilly / Farja I Ayala / Ulla Schellhaas / Juri Rappsilber / Clemens Plaschka /
PubMed 要旨	Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the ...Newly made mRNAs are processed and packaged into mature ribonucleoprotein complexes (mRNPs) and are recognized by the essential transcription-export complex (TREX) for nuclear export. However, the mechanisms of mRNP recognition and three-dimensional mRNP organization are poorly understood. Here we report cryo-electron microscopy and tomography structures of reconstituted and endogenous human mRNPs bound to the 2-MDa TREX complex. We show that mRNPs are recognized through multivalent interactions between the TREX subunit ALYREF and mRNP-bound exon junction complexes. Exon junction complexes can multimerize through ALYREF, which suggests a mechanism for mRNP organization. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes. These results reveal how TREX may simultaneously recognize, compact and protect mRNAs to promote their packaging for nuclear export. The organization of mRNP globules provides a framework to understand how mRNP architecture facilitates mRNA biogenesis and export.
リンク	Nature / PubMed:37020021 / PubMed Central
手法	EM (単粒子) / EM (トモグラフィー)
解像度	2.4 - 7.5 Å
構造データ	14803 7znj EMDB-14803, PDB-7znj: Structure of an ALYREF-exon junction complex hexamer 手法: EM (単粒子) / 解像度: 2.4 Å 14804 7znk EMDB-14804: Structure of an endogenous human TREX complex bound to mRNA, composite map PDB-7znk: Structure of an endogenous human TREX complex bound to mRNA 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-14805: Structure of an endogenous human TREX complex bound to mRNA, map A 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-14806: Structure of an endogenous human TREX complex bound to mRNA, map B 手法: EM (単粒子) / 解像度: 5.5 Å EMDB-14807: Structure of an endogenous human TREX complex bound to mRNA, map C 手法: EM (単粒子) / 解像度: 7.5 Å 14808 7znl EMDB-14808: Structure of the human TREX core THO-UAP56 complex (map D) PDB-7znl: Structure of the human TREX core THO-UAP56 complex 手法: EM (単粒子) / 解像度: 3.45 Å EMDB-14809: Structure of the human TREX core THO-UAP56 complex (map E) 手法: EM (単粒子) / 解像度: 3.9 Å EMDB-16633: Structure of an ALYREF-exon junction complex hexamer, obtained from TREX-EJC-RNA sample 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-16753: Tomogram of purified human TREX-mRNPs 手法: EM (トモグラフィー) EMDB-17215: Composite map of the human TREX core THO-UAP56 complex 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体*YM
由来	homo sapiens (ヒト) human (ヒト)
キーワード	GENE REGULATION (遺伝子発現の調節) / transcription-exort complex / TREX / splicing / exon junction complex / EJC / RNA export / RNA binding proteins / transcription and export complex / RNA packaging / RNA binding protein (RNA結合タンパク質) / gene expression (遺伝子発現)