EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 28, Issue 2, Page 210-219, Year 2021
掲載日	2021年2月8日
著者	Mathias Girbig / Agata D Misiaszek / Matthias K Vorländer / Aleix Lafita / Helga Grötsch / Florence Baudin / Alex Bateman / Christoph W Müller /
PubMed 要旨	RNA polymerase III (Pol III) synthesizes transfer RNAs and other short, essential RNAs. Human Pol III misregulation is linked to tumor transformation, neurodegenerative and developmental disorders, ...RNA polymerase III (Pol III) synthesizes transfer RNAs and other short, essential RNAs. Human Pol III misregulation is linked to tumor transformation, neurodegenerative and developmental disorders, and increased sensitivity to viral infections. Here, we present cryo-electron microscopy structures at 2.8 to 3.3 Å resolution of transcribing and unbound human Pol III. We observe insertion of the TFIIS-like subunit RPC10 into the polymerase funnel, providing insights into how RPC10 triggers transcription termination. Our structures resolve elements absent from Saccharomyces cerevisiae Pol III such as the winged-helix domains of RPC5 and an iron-sulfur cluster, which tethers the heterotrimer subcomplex to the core. The cancer-associated RPC7α isoform binds the polymerase clamp, potentially interfering with Pol III inhibition by tumor suppressor MAF1, which may explain why overexpressed RPC7α enhances tumor transformation. Finally, the human Pol III structure allows mapping of disease-related mutations and may contribute to the development of inhibitors that selectively target Pol III for therapeutic interventions.
リンク	Nat Struct Mol Biol / PubMed:33558764 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.4 Å
構造データ	11673 7a6h EMDB-11673, PDB-7a6h: Cryo-EM structure of human apo RNA Polymerase III 手法: EM (単粒子) / 解像度: 3.3 Å 11736 7ae1 EMDB-11736, PDB-7ae1: Cryo-EM structure of human RNA Polymerase III elongation complex 1 手法: EM (単粒子) / 解像度: 2.8 Å EMDB-11737: Human RNA Polymerase III Elongation Complex, Map C 手法: EM (単粒子) / 解像度: 3.1 Å 11738 7ae3 EMDB-11738, PDB-7ae3: Cryo-EM structure of human RNA Polymerase III elongation complex 3 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-11739: Cryo-EM structure of human RNA Polymerase III Elongation Complex, Map E 手法: EM (単粒子) / 解像度: 2.9 Å EMDB-11740: Cryo-EM structure of human RNA Polymerase III Elongation Complex, map F 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-11741: Cryo-EM structure of human RNA Polymerase III, map G 手法: EM (単粒子) / 解像度: 2.9 Å 11742 7aea EMDB-11742, PDB-7aea: Cryo-EM structure of human RNA Polymerase III elongation complex 2 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-SF4: IRON/SULFUR CLUSTER
由来	homo sapiens (ヒト) synthetic construct (人工物)
キーワード	TRANSCRIPTION / Human RNA polymerase III / Synthesis of short RNAs / apo complex / HUMAN / SHORT RNAs