+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-11737 | |||||||||
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Title | Human RNA Polymerase III Elongation Complex, Map C | |||||||||
Map data | Cryo-EM map of human RNA Polymerase III Elongation complex, map C, unsharpened | |||||||||
Sample |
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Function / homology | Function and homology information snRNA transcription by RNA polymerase III / RNA Polymerase III Chain Elongation / RNA Polymerase III Transcription Termination / calcitonin gene-related peptide receptor activity / DNA/RNA hybrid binding / regulation of transcription by RNA polymerase I / regulation of transcription by RNA polymerase III / RPAP3/R2TP/prefoldin-like complex / DNA polymerase III complex / Cytosolic sensors of pathogen-associated DNA ...snRNA transcription by RNA polymerase III / RNA Polymerase III Chain Elongation / RNA Polymerase III Transcription Termination / calcitonin gene-related peptide receptor activity / DNA/RNA hybrid binding / regulation of transcription by RNA polymerase I / regulation of transcription by RNA polymerase III / RPAP3/R2TP/prefoldin-like complex / DNA polymerase III complex / Cytosolic sensors of pathogen-associated DNA / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / RNA Polymerase III Abortive And Retractive Initiation / positive regulation of innate immune response / Abortive elongation of HIV-1 transcript in the absence of Tat / nucleobase-containing compound metabolic process / MicroRNA (miRNA) biogenesis / FGFR2 alternative splicing / RNA Polymerase I Transcription Termination / Viral Messenger RNA Synthesis / Signaling by FGFR2 IIIa TM / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / termination of RNA polymerase III transcription / PIWI-interacting RNA (piRNA) biogenesis / RNA polymerase III activity / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / transcription initiation at RNA polymerase III promoter / mRNA Splicing - Minor Pathway / RNA Polymerase I Transcription Initiation / transcription by RNA polymerase I / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / transcription by RNA polymerase III / Processing of Capped Intron-Containing Pre-mRNA / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / RNA polymerase II transcribes snRNA genes / neuropeptide signaling pathway / transcription elongation by RNA polymerase I / Tat-mediated elongation of the HIV-1 transcript / tRNA transcription by RNA polymerase III / RNA polymerase I activity / Formation of HIV-1 elongation complex containing HIV-1 Tat / RNA polymerase I complex / RNA polymerase III complex / Formation of HIV elongation complex in the absence of HIV Tat / RNA polymerase II, core complex / RNA Polymerase II Transcription Elongation / Formation of RNA Pol II elongation complex / Inhibition of DNA recombination at telomere / RNA Polymerase II Pre-transcription Events / positive regulation of interferon-beta production / mRNA Splicing - Major Pathway / acrosomal vesicle / RNA Polymerase I Promoter Escape / Transcriptional regulation by small RNAs / protein-DNA complex / TP53 Regulates Transcription of DNA Repair Genes / NoRC negatively regulates rRNA expression / B-WICH complex positively regulates rRNA expression / Transcription-Coupled Nucleotide Excision Repair (TC-NER) / Formation of TC-NER Pre-Incision Complex / ribonucleoside binding / fibrillar center / Activation of anterior HOX genes in hindbrain development during early embryogenesis / DNA-directed 5'-3' RNA polymerase activity / DNA-directed RNA polymerase / Dual incision in TC-NER / Gap-filling DNA repair synthesis and ligation in TC-NER / double-stranded DNA binding / single-stranded DNA binding / 4 iron, 4 sulfur cluster binding / defense response to virus / Estrogen-dependent gene expression / nucleic acid binding / cell population proliferation / transcription by RNA polymerase II / protein dimerization activity / nuclear body / protein stabilization / intracellular membrane-bounded organelle / innate immune response / chromatin binding / centrosome / DNA-templated transcription / nucleotide binding / magnesium ion binding / DNA binding / zinc ion binding Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.1 Å | |||||||||
Authors | Girbig M / Misiaszek AD / Vorlaender MK / Mueller CW | |||||||||
Citation | Journal: Nat Struct Mol Biol / Year: 2021 Title: Cryo-EM structures of human RNA polymerase III in its unbound and transcribing states. Authors: Mathias Girbig / Agata D Misiaszek / Matthias K Vorländer / Aleix Lafita / Helga Grötsch / Florence Baudin / Alex Bateman / Christoph W Müller / Abstract: RNA polymerase III (Pol III) synthesizes transfer RNAs and other short, essential RNAs. Human Pol III misregulation is linked to tumor transformation, neurodegenerative and developmental disorders, ...RNA polymerase III (Pol III) synthesizes transfer RNAs and other short, essential RNAs. Human Pol III misregulation is linked to tumor transformation, neurodegenerative and developmental disorders, and increased sensitivity to viral infections. Here, we present cryo-electron microscopy structures at 2.8 to 3.3 Å resolution of transcribing and unbound human Pol III. We observe insertion of the TFIIS-like subunit RPC10 into the polymerase funnel, providing insights into how RPC10 triggers transcription termination. Our structures resolve elements absent from Saccharomyces cerevisiae Pol III such as the winged-helix domains of RPC5 and an iron-sulfur cluster, which tethers the heterotrimer subcomplex to the core. The cancer-associated RPC7α isoform binds the polymerase clamp, potentially interfering with Pol III inhibition by tumor suppressor MAF1, which may explain why overexpressed RPC7α enhances tumor transformation. Finally, the human Pol III structure allows mapping of disease-related mutations and may contribute to the development of inhibitors that selectively target Pol III for therapeutic interventions. | |||||||||
History |
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-Structure visualization
Movie |
Movie viewer |
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Structure viewer | EM map: SurfViewMolmilJmol/JSmol |
Supplemental images |
-Downloads & links
-EMDB archive
Map data | emd_11737.map.gz | 113.6 MB | EMDB map data format | |
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Header (meta data) | emd-11737-v30.xml emd-11737.xml | 20 KB 20 KB | Display Display | EMDB header |
FSC (resolution estimation) | emd_11737_fsc.xml | 11.9 KB | Display | FSC data file |
Images | emd_11737.png | 76.3 KB | ||
Masks | emd_11737_msk_1.map | 144.7 MB | Mask map | |
Others | emd_11737_additional_1.map.gz emd_11737_half_map_1.map.gz emd_11737_half_map_2.map.gz | 113.1 MB 114.1 MB 114.2 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-11737 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-11737 | HTTPS FTP |
-Validation report
Summary document | emd_11737_validation.pdf.gz | 465.3 KB | Display | EMDB validaton report |
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Full document | emd_11737_full_validation.pdf.gz | 464.5 KB | Display | |
Data in XML | emd_11737_validation.xml.gz | 18 KB | Display | |
Arichive directory | https://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11737 ftp://ftp.pdbj.org/pub/emdb/validation_reports/EMD-11737 | HTTPS FTP |
-Related structure data
Related structure data | 7a6hC 7ae1C 7ae3C 7aeaC C: citing same article (ref.) |
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Similar structure data | |
EM raw data | EMPIAR-10697 (Title: Cryo-EM structures of human RNA Polymerase III / Data size: 3.3 TB Data #1: LZW-TIFF compressed multiframe micrographs of human RNA Pol III EC [micrographs - multiframe] Data #2: Initially picked particles of human RNA Pol III EC [picked particles - single frame - processed] Data #3: Polished particles of human RNA Pol III EC (final reconstruction) [picked particles - single frame - processed] Data #4: LZW-TIFF compressed multiframe micrographs of human RNA Pol III apo [micrographs - multiframe] Data #5: Initially picked particles of human RNA Pol III apo [picked particles - single frame - processed] Data #6: Polished particles of human RNA Pol III apo (final reconstruction) [picked particles - single frame - processed]) |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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Related items in Molecule of the Month |
-Map
File | Download / File: emd_11737.map.gz / Format: CCP4 / Size: 144.7 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Cryo-EM map of human RNA Polymerase III Elongation complex, map C, unsharpened | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.05 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | emd_11737_msk_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Additional map: Cryo-EM map of human RNA Polymerase III Elongation...
File | emd_11737_additional_1.map | ||||||||||||
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Annotation | Cryo-EM map of human RNA Polymerase III Elongation complex, map C, locally sharpened via LocalDeblur | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_11737_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #1
File | emd_11737_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Human RNA Polymerase III Elongation Complex
Entire | Name: Human RNA Polymerase III Elongation Complex |
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Components |
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-Supramolecule #1: Human RNA Polymerase III Elongation Complex
Supramolecule | Name: Human RNA Polymerase III Elongation Complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#20 |
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Source (natural) | Organism: Homo sapiens (human) / Strain: HEK293F |
Molecular weight | Theoretical: 776 kDa/nm |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Concentration | 2 mg/mL | ||||||||||||
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Buffer | pH: 7.5 Component:
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Vitrification | Cryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV / Details: Wait time 10 s Blot force 4 Blot time 4 s. |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 40.4 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | C2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.75 µm / Nominal magnification: 130000 |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |