EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Ebola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers.
ジャーナル・号・ページ	Elife, Vol. 9, Year 2020
掲載日	2020年10月5日
著者	William Wan / Mairi Clarke / Michael J Norris / Larissa Kolesnikova / Alexander Koehler / Zachary A Bornholdt / Stephan Becker / Erica Ollmann Saphire / John Ag Briggs /
PubMed 要旨	Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of ...Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.
リンク	Elife / PubMed:33016878 / PubMed Central
手法	EM (サブトモグラム平均) / X線回折
解像度	2.46 - 12.8 Å
構造データ	EMDB-11660: Structure of VP40 matrix layer in EBOV NP-VP24-VP35-VP40 VLPs 手法: EM (サブトモグラム平均) / 解像度: 10.2 Å EMDB-11661: Structure of VP40 matrix layer in EBOV VP40 VLPs 手法: EM (サブトモグラム平均) / 解像度: 9.8 Å EMDB-11662: Structure of VP40 matrix layer in EBOV VP40-GP VLPs 手法: EM (サブトモグラム平均) / 解像度: 9.9 Å EMDB-11663: Structure of VP40 matrix layer in intact MARV virions 手法: EM (サブトモグラム平均) / 解像度: 9.6 Å EMDB-11664: Structure of VP40 matrix layer in MARV VP40 VLPs 手法: EM (サブトモグラム平均) / 解像度: 10.8 Å EMDB-11665: Structure of GP in EBOV VP40-GP VLPs 手法: EM (サブトモグラム平均) / 解像度: 12.8 Å PDB-7jzj: Crystal structure demonstrating CTD-CTD interactions of Zaire Ebola virus VP40 dimer 手法: X-RAY DIFFRACTION / 解像度: 2.46 Å PDB-7jzt: Low resolution crystal structure of Zaire Ebola virus VP40 in space group P6422 手法: X-RAY DIFFRACTION / 解像度: 3.77 Å
化合物	ChemComp-1PE: PENTAETHYLENE GLYCOL / ペンタエチレングリコ-ル / 沈殿剤YM ChemComp-HOH*: WATER
由来	Ebola virus - Mayinga (エボラウイルス) Marburg virus - Musoke (ウイルス) zaire ebolavirus (strain mayinga-76) (エボラウイルス)
キーワード	VIRAL PROTEIN / VP40 / matrix protein / Ebola virus