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-Structure paper
タイトル | Evolutionary shift toward protein-based architecture in trypanosomal mitochondrial ribosomes. |
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ジャーナル・号・ページ | Science, Vol. 362, Issue 6413, Year 2018 |
掲載日 | 2018年10月26日 |
著者 | David J F Ramrath / Moritz Niemann / Marc Leibundgut / Philipp Bieri / Céline Prange / Elke K Horn / Alexander Leitner / Daniel Boehringer / André Schneider / Nenad Ban / |
PubMed 要旨 | Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , ...Ribosomal RNA (rRNA) plays key functional and architectural roles in ribosomes. Using electron microscopy, we determined the atomic structure of a highly divergent ribosome found in mitochondria of , a unicellular parasite that causes sleeping sickness in humans. The trypanosomal mitoribosome features the smallest rRNAs and contains more proteins than all known ribosomes. The structure shows how the proteins have taken over the role of architectural scaffold from the rRNA: They form an autonomous outer shell that surrounds the entire particle and stabilizes and positions the functionally important regions of the rRNA. Our results also reveal the "minimal" set of conserved rRNA and protein components shared by all ribosomes that help us define the most essential functional elements. |
リンク | Science / PubMed:30213880 |
手法 | EM (単粒子) |
解像度 | 3.08 - 7.8 Å |
構造データ | EMDB-0229, PDB-6hiv: EMDB-0230, PDB-6hiw: EMDB-0231, PDB-6hix: |
化合物 | ChemComp-ZN: ChemComp-SPD: ChemComp-UTP: ChemComp-MG: ChemComp-GTP: ChemComp-SPM: ChemComp-NAD: ChemComp-SER: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / mitoribosome / translation / Trypanosoma / large ribosomal subunit / small ribosomal subunit / 12S rRNA / 9S rRNA / ribosomal protein / mitochondrial initiation factor IF-3 |