EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cutting antiparallel DNA strands in a single active site.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 27, Issue 2, Page 119-126, Year 2020
掲載日	2020年2月3日
著者	Xuemin Chen / Yanxiang Cui / Robert B Best / Huaibin Wang / Z Hong Zhou / Wei Yang / Martin Gellert /
PubMed 要旨	A single enzyme active site that catalyzes multiple reactions is a well-established biochemical theme, but how one nuclease site cleaves both DNA strands of a double helix has not been well ...A single enzyme active site that catalyzes multiple reactions is a well-established biochemical theme, but how one nuclease site cleaves both DNA strands of a double helix has not been well understood. In analyzing site-specific DNA cleavage by the mammalian RAG1-RAG2 recombinase, which initiates V(D)J recombination, we find that the active site is reconfigured for the two consecutive reactions and the DNA double helix adopts drastically different structures. For initial nicking of the DNA, a locally unwound and unpaired DNA duplex forms a zipper via alternating interstrand base stacking, rather than melting as generally thought. The second strand cleavage and formation of a hairpin-DNA product requires a global scissor-like movement of protein and DNA, delivering the scissile phosphate into the rearranged active site.
リンク	Nat Struct Mol Biol / PubMed:32015552 / PubMed Central
手法	EM (単粒子)
解像度	3.15 - 4.3 Å
構造データ	20030 6oem EMDB-20030, PDB-6oem: Cryo-EM structure of mouse RAG1/2 PRC complex (DNA0) 手法: EM (単粒子) / 解像度: 3.6 Å 20031 6oen EMDB-20031, PDB-6oen: Cryo-EM structure of mouse RAG1/2 PRC complex (DNA1) 手法: EM (単粒子) / 解像度: 4.3 Å 20032 6oeo EMDB-20032, PDB-6oeo: Cryo-EM structure of mouse RAG1/2 NFC complex (DNA1) 手法: EM (単粒子) / 解像度: 3.69 Å 20033 6oep EMDB-20033, PDB-6oep: Cryo-EM structure of mouse RAG1/2 12RSS-NFC/23RSS-PRC complex (DNA1) 手法: EM (単粒子) / 解像度: 3.7 Å 20034 6oeq EMDB-20034, PDB-6oeq: Cryo-EM structure of mouse RAG1/2 12RSS-PRC/23RSS-NFC complex (DNA1) 手法: EM (単粒子) / 解像度: 4.3 Å 20035 6oer EMDB-20035, PDB-6oer: Cryo-EM structure of mouse RAG1/2 NFC complex (DNA2) 手法: EM (単粒子) / 解像度: 3.29 Å EMDB-20038: Cryo-EM structure of mouse RAG1/2 NFC complex (DNA2) with C2 symmetry 手法: EM (単粒子) / 解像度: 3.15 Å EMDB-20039: Cryo-EM structure of mouse RAG1/2 NFC complex (DNA1) with C2 symmetry 手法: EM (単粒子) / 解像度: 3.44 Å 21003 6v0v EMDB-21003, PDB-6v0v: Cryo-EM structure of mouse WT RAG1/2 NFC complex (DNA0) 手法: EM (単粒子) / 解像度: 3.61 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	mus musculus (ハツカネズミ) homo sapiens (ヒト) escherichia coli k-12 (大腸菌) equus caballus (ウマ) escherichia coli (大腸菌)
キーワード	RECOMBINATION/DNA / V(D)J recombination (V(D)J遺伝子再構成) / DNA Transposition / RAG / SCID / RECOMBINATION (遺伝的組換え) / RECOMBINATION-DNA complex