EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM Reveals How Human Cytoplasmic Dynein Is Auto-inhibited and Activated.
ジャーナル・号・ページ	Cell, Vol. 169, Issue 7, Page 1303-1314.e18, Year 2017
掲載日	2017年6月15日
著者	Kai Zhang / Helen E Foster / Arnaud Rondelet / Samuel E Lacey / Nadia Bahi-Buisson / Alexander W Bird / Andrew P Carter /
PubMed 要旨	Cytoplasmic dynein-1 binds dynactin and cargo adaptor proteins to form a transport machine capable of long-distance processive movement along microtubules. However, it is unclear why dynein-1 moves ...Cytoplasmic dynein-1 binds dynactin and cargo adaptor proteins to form a transport machine capable of long-distance processive movement along microtubules. However, it is unclear why dynein-1 moves poorly on its own or how it is activated by dynactin. Here, we present a cryoelectron microscopy structure of the complete 1.4-megadalton human dynein-1 complex in an inhibited state known as the phi-particle. We reveal the 3D structure of the cargo binding dynein tail and show how self-dimerization of the motor domains locks them in a conformation with low microtubule affinity. Disrupting motor dimerization with structure-based mutagenesis drives dynein-1 into an open form with higher affinity for both microtubules and dynactin. We find the open form is also inhibited for movement and that dynactin relieves this by reorienting the motor domains to interact correctly with microtubules. Our model explains how dynactin binding to the dynein-1 tail directly stimulates its motor activity.
リンク	Cell / PubMed:28602352 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 15.0 Å
構造データ	3698 5nug EMDB-3698, PDB-5nug: Motor domains from human cytoplasmic dynein-1 in the phi-particle conformation 手法: EM (単粒子) / 解像度: 3.8 Å 3703 5nvs EMDB-3703, PDB-5nvs: Human cytoplasmic dynein-1 tail in the twisted N-terminus state 手法: EM (単粒子) / 解像度: 8.4 Å EMDB-3704: Human cytoplasmic dynein-1 tail in the parallel state 手法: EM (単粒子) / 解像度: 12.0 Å 3705 5nvu EMDB-3705, PDB-5nvu: Full length human cytoplasmic dynein-1 in the phi-particle conformation 手法: EM (単粒子) / 解像度: 15.0 Å 3706 5nw4 EMDB-3706, PDB-5nw4: Human cytoplasmic dynein-1 bound to dynactin and an N-terminal construct of BICD2 手法: EM (単粒子) / 解像度: 8.7 Å EMDB-3707: Locally refined human cytoplasmic dynein-1 tail bound to dynactin and an N-terminal construct of BICD2 手法: EM (単粒子) / 解像度: 12.0 Å
化合物	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	homo sapiens (ヒト) sus scrofa (ブタ) Pig (ブタ)
キーワード	MOTOR PROTEIN (モータータンパク質) / dynein (ダイニン) / motor domain (機関 (機械)) / AAA+ / tail complex (尾) / dynein-1 (ダイニン) / phi-particle / dynactin (ダイナクチン) / BICD