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Structure paper

タイトル	Molecular structure of the intact bacterial flagellar basal body.
ジャーナル・号・ページ	Nat Microbiol, Vol. 6, Issue 6, Page 712-721, Year 2021
掲載日	2021年4月30日
著者	Steven Johnson / Emily J Furlong / Justin C Deme / Ashley L Nord / Joseph J E Caesar / Fabienne F V Chevance / Richard M Berry / Kelly T Hughes / Susan M Lea /
PubMed 要旨	The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the ...The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids. Here, we present structures of the intact Salmonella flagellar basal body, encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 Å. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine.
リンク	Nat Microbiol / PubMed:33931760 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 3.8 Å
構造データ	12183 7bgl EMDB-12183, PDB-7bgl: Salmonella LP ring 26 mer refined in C26 map 手法: EM (単粒子) / 解像度: 2.2 Å 12190 7bhq EMDB-12190, PDB-7bhq: In situ assembled Salmonella FlgD hook cap complex 手法: EM (単粒子) / 解像度: 3.2 Å 12192 7bin EMDB-12192, PDB-7bin: Salmonella export gate and rod refined in focussed C1 map 手法: EM (単粒子) / 解像度: 3.2 Å 12193 7bj2 EMDB-12193, PDB-7bj2: Salmonella flagellar basal body assembly intermediate - P ring alone structure 手法: EM (単粒子) / 解像度: 3.0 Å 12195 7bk0 EMDB-12195, PDB-7bk0: Salmonella FliF ring (34mer) in intact basal body - C1 手法: EM (単粒子) / 解像度: 3.8 Å 12603 7nvg EMDB-12603, PDB-7nvg: Salmonella flagellar basal body refined in C1 map 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-TLW: (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-4,5-bis(oxidanyl)oxane-2-carboxylic acid ChemComp-TQN: [(3~{R})-1-[[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-[[(2~{R},3~{R},4~{R},5~{S},6~{R})-3-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-(hydroxymethyl)-5-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]oxymethyl]-5-oxidanyl-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-2-phosphonooxy-oxan-3-yl]amino]-1-oxidanylidene-tetradecan-3-yl] hexadecanoate / 6-O-[3-O-[(3R)-1-オキソ-3-(ミリストイルオキシ)テトラデシル]-4-O-ホスホノ-2-[[(3R)(以下略)
由来	salmonella enterica subsp. enterica serovar typhi (サルモネラ菌) salmonella typhimurium (strain lt2 / sgsc1412 / atcc 700720) (サルモネラ菌) salmonella typhi (サルモネラ菌) salmonella enterica subsp. enterica serovar typhimurium (サルモネラ菌)
キーワード	MEMBRANE PROTEIN / bacterial flagellum LP ring salmonella / bacterial flagellum hook cap salmonella flagellar assembly / PROTEIN TRANSPORT / bacterial flagellum rod bacterial flagellum export gate basal body / bacterial flagellum basal body MS-ring / bacterial flagellum / flagella / basal body