Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structures of T7 bacteriophage portal and tail suggest a viral DNA retention and ejection mechanism.
Journal, issue, pages	Nat Commun, Vol. 10, Issue 1, Page 3746, Year 2019
Publish date	Aug 20, 2019
Authors	Ana Cuervo / Montserrat Fàbrega-Ferrer / Cristina Machón / José Javier Conesa / Francisco J Fernández / Rosa Pérez-Luque / Mar Pérez-Ruiz / Joan Pous / M Cristina Vega / José L Carrascosa / Miquel Coll /
PubMed Abstract	Double-stranded DNA bacteriophages package their genome at high pressure inside a procapsid through the portal, an oligomeric ring protein located at a unique capsid vertex. Once the DNA has been ...Double-stranded DNA bacteriophages package their genome at high pressure inside a procapsid through the portal, an oligomeric ring protein located at a unique capsid vertex. Once the DNA has been packaged, the tail components assemble on the portal to render the mature infective virion. The tail tightly seals the ejection conduit until infection, when its interaction with the host membrane triggers the opening of the channel and the viral genome is delivered to the host cell. Using high-resolution cryo-electron microscopy and X-ray crystallography, here we describe various structures of the T7 bacteriophage portal and fiber-less tail complex, which suggest a possible mechanism for DNA retention and ejection: a portal closed conformation temporarily retains the genome before the tail is assembled, whereas an open portal is found in the tail. Moreover, a fold including a seven-bladed β-propeller domain is described for the nozzle tail protein.
External links	Nat Commun / PubMed:31431626 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	3.33 - 5.8 Å
Structure data	EMDB-4667: Cryo-EM structure of T7 bacteriophage portal protein, 13mer, closed valve Method: EM (single particle) / Resolution: 5.8 Å 4669 6qxm EMDB-4669: Bacteriophage packaging protein PDB-6qxm: Cryo-EM structure of T7 bacteriophage portal protein, 12mer, open valve Method: EM (single particle) / Resolution: 4.1 Å 4706 6r21 EMDB-4706: Bacteriophage ejection complex PDB-6r21: Cryo-EM structure of T7 bacteriophage fiberless tail complex Method: EM (single particle) / Resolution: 3.33 Å PDB-6qwp: Crystal structure of T7 bacteriophage portal protein, 13mer, closed valve Method: X-RAY DIFFRACTION / Resolution: 3.4 Å PDB-6qx5: Crystal structure of T7 bacteriophage portal protein, 12mer, closed valve Method: X-RAY DIFFRACTION / Resolution: 3.6 Å
Source	enterobacteria phage t7 (virus)
Keywords	VIRAL PROTEIN / DNA packaging / viral complex / DNA ejection