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Title | Molecular basis of translation termination at noncanonical stop codons in human mitochondria. |
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Journal, issue, pages | Science, Vol. 380, Issue 6644, Page 531-536, Year 2023 |
Publish date | May 5, 2023 |
Authors | Martin Saurer / Marc Leibundgut / Hima Priyanka Nadimpalli / Alain Scaiola / Tanja Schönhut / Richard G Lee / Stefan J Siira / Oliver Rackham / René Dreos / Tea Lenarčič / Eva Kummer / David Gatfield / Aleksandra Filipovska / Nenad Ban / |
PubMed Abstract | The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the ...The genetic code that specifies the identity of amino acids incorporated into proteins during protein synthesis is almost universally conserved. Mitochondrial genomes feature deviations from the standard genetic code, including the reassignment of two arginine codons to stop codons. The protein required for translation termination at these noncanonical stop codons to release the newly synthesized polypeptides is not currently known. In this study, we used gene editing and ribosomal profiling in combination with cryo-electron microscopy to establish that mitochondrial release factor 1 (mtRF1) detects noncanonical stop codons in human mitochondria by a previously unknown mechanism of codon recognition. We discovered that binding of mtRF1 to the decoding center of the ribosome stabilizes a highly unusual conformation in the messenger RNA in which the ribosomal RNA participates in specific recognition of the noncanonical stop codons. |
External links | Science / PubMed:37141370 |
Methods | EM (single particle) |
Resolution | 2.9 - 3.6 Å |
Structure data | EMDB-16894, PDB-8oin: EMDB-16895, PDB-8oip: EMDB-16896, PDB-8oiq: EMDB-16897, PDB-8oir: EMDB-16898, PDB-8ois: EMDB-16899, PDB-8oit: |
Chemicals | ChemComp-MG: ChemComp-K: ChemComp-PHE: ChemComp-ZN: ChemComp-FES: ChemComp-SPM: ChemComp-SPD: ChemComp-FME: ChemComp-ATP: ChemComp-GDP: ChemComp-HOH: ChemComp-FS2: ChemComp-VAL: ChemComp-MET: |
Source |
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Keywords | RIBOSOME / mammalian mitochondrial ribosome / release factor mtRF1 / non-canonical stop codon / human mitochondrial ribosome |