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Title | Structure of the transcribing RNA polymerase II-Elongin complex. |
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Journal, issue, pages | Nat Struct Mol Biol, Vol. 30, Issue 12, Page 1925-1935, Year 2023 |
Publish date | Nov 6, 2023 |
Authors | Ying Chen / Goran Kokic / Christian Dienemann / Olexandr Dybkov / Henning Urlaub / Patrick Cramer / |
PubMed Abstract | Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to ...Elongin is a heterotrimeric elongation factor for RNA polymerase (Pol) II transcription that is conserved among metazoa. Here, we report three cryo-EM structures of human Elongin bound to transcribing Pol II. The structures show that Elongin subunit ELOA binds the RPB2 side of Pol II and anchors the ELOB-ELOC subunit heterodimer. ELOA contains a 'latch' that binds between the end of the Pol II bridge helix and funnel helices, thereby inducing a conformational change near the polymerase active center. The latch is required for the elongation-stimulatory activity of Elongin, but not for Pol II binding, indicating that Elongin functions by allosterically regulating the conformational mobility of the polymerase active center. Elongin binding to Pol II is incompatible with association of the super elongation complex, PAF1 complex and RTF1, which also contain an elongation-stimulatory latch element. |
External links | Nat Struct Mol Biol / PubMed:37932450 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.69 - 4.2 Å |
Structure data | EMDB-16828: Structure of the mammalian Pol II-SPT6 complex, Struture 4 (Global map) EMDB-16829: Structure of the mammalian Pol II-SPT6 complex, Structure 4 (Focused map for SPT6) EMDB-16830: Structure of the mammalian Pol II-Elongin complex, lacking ELOA latch (Focused map for elongin, map 5) EMDB-16831: Structure of the mammalian Pol II-Elongin complex, lacking ELOA latch (Focused map for Pol II core, Map 6) EMDB-16832: Structure of the mammalian Pol II-Elongin complex, lacking ELOA latch (Focused map for Pol II stalk, map 7) EMDB-16833: Structure of the mammalian Pol II-SPT6-Elongin complex, lacking the ELOA latch (global map for composite map 2) EMDB-16834: Structure of the mammalian Pol II-SPT6-Elongin complex, lacking ELOA latch (SPT6 focused map for composite map 2) EMDB-16835: Structure of the mammalian Pol II-SPT6 complex (composite map) EMDB-16836: Structure of the Pol II-SPT6-Elongin complex (structure 1, global map) EMDB-16837: Structure of the mammalian Pol II-SPT6-Elongin complex, lacking ELOA latch (composite map 2) EMDB-16838: Structure of the mammalian Pol II-Elongin complex, lacking the ELOA latch (composite map 1 for structure 2) EMDB-16839: Structure of the mammalian Pol II-Elongin complex, lacking the ELOA latch (local resolution filtered map) EMDB-16840: Structure of the mammalian Pol II-SPT6-Elongin complex, Structure 1 (local resolution filtered map) |
Chemicals | ChemComp-MG: ChemComp-ZN: |
Source |
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Keywords | TRANSCRIPTION / Transcription elongation / Elongin / RNA polymerase II |