Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Molecular structure of the intact bacterial flagellar basal body.
Journal, issue, pages	Nat Microbiol, Vol. 6, Issue 6, Page 712-721, Year 2021
Publish date	Apr 30, 2021
Authors	Steven Johnson / Emily J Furlong / Justin C Deme / Ashley L Nord / Joseph J E Caesar / Fabienne F V Chevance / Richard M Berry / Kelly T Hughes / Susan M Lea /
PubMed Abstract	The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the ...The bacterial flagellum is a macromolecular protein complex that enables motility in many species. Bacterial flagella self-assemble a strong, multicomponent drive shaft that couples rotation in the inner membrane to the micrometre-long flagellar filament that powers bacterial swimming in viscous fluids. Here, we present structures of the intact Salmonella flagellar basal body, encompassing the inner membrane rotor, drive shaft and outer-membrane bushing, solved using cryo-electron microscopy to resolutions of 2.2-3.7 Å. The structures reveal molecular details of how 173 protein molecules of 13 different types assemble into a complex spanning two membranes and a cell wall. The helical drive shaft at one end is intricately interwoven with the rotor component with both the export gate complex and the proximal rod forming interactions with the MS-ring. At the other end, the drive shaft distal rod passes through the LP-ring bushing complex, which functions as a molecular bearing anchored in the outer membrane through interactions with the lipopolysaccharide. The in situ structure of a protein complex capping the drive shaft provides molecular insights into the assembly process of this molecular machine.
External links	Nat Microbiol / PubMed:33931760 / PubMed Central
Methods	EM (single particle)
Resolution	2.2 - 3.8 Å
Structure data	12183 7bgl EMDB-12183, PDB-7bgl: Salmonella LP ring 26 mer refined in C26 map Method: EM (single particle) / Resolution: 2.2 Å 12190 7bhq EMDB-12190, PDB-7bhq: In situ assembled Salmonella FlgD hook cap complex Method: EM (single particle) / Resolution: 3.2 Å 12192 7bin EMDB-12192, PDB-7bin: Salmonella export gate and rod refined in focussed C1 map Method: EM (single particle) / Resolution: 3.2 Å 12193 7bj2 EMDB-12193, PDB-7bj2: Salmonella flagellar basal body assembly intermediate - P ring alone structure Method: EM (single particle) / Resolution: 3.0 Å 12195 7bk0 EMDB-12195, PDB-7bk0: Salmonella FliF ring (34mer) in intact basal body - C1 Method: EM (single particle) / Resolution: 3.8 Å 12603 7nvg EMDB-12603, PDB-7nvg: Salmonella flagellar basal body refined in C1 map Method: EM (single particle) / Resolution: 3.7 Å
Chemicals	ChemComp-TLW: (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-4,5-bis(oxidanyl)oxane-2-carboxylic acid ChemComp-TQN: [(3~{R})-1-[[(2~{R},3~{R},4~{R},5~{S},6~{R})-6-[[(2~{R},3~{R},4~{R},5~{S},6~{R})-3-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-(hydroxymethyl)-5-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]oxymethyl]-5-oxidanyl-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-2-phosphonooxy-oxan-3-yl]amino]-1-oxidanylidene-tetradecan-3-yl] hexadecanoate
Source	salmonella enterica subsp. enterica serovar typhi (bacteria) salmonella typhimurium (strain lt2 / sgsc1412 / atcc 700720) (bacteria) salmonella typhi (bacteria) salmonella enterica subsp. enterica serovar typhimurium (bacteria)
Keywords	MEMBRANE PROTEIN / bacterial flagellum LP ring salmonella / bacterial flagellum hook cap salmonella flagellar assembly / PROTEIN TRANSPORT / bacterial flagellum rod bacterial flagellum export gate basal body / bacterial flagellum basal body MS-ring / bacterial flagellum / flagella / basal body