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TitleAtomic models of the Toxoplasma cell invasion machinery.
Journal, issue, pagesNat Struct Mol Biol, Year 2025
Publish dateDec 9, 2025
AuthorsJianwei Zeng / Yong Fu / Pengge Qian / Wei Huang / Qingwei Niu / Wandy L Beatty / Alan Brown / L David Sibley / Rui Zhang /
PubMed AbstractApicomplexan parasites, responsible for toxoplasmosis, cryptosporidiosis and malaria, invade host cells through a unique gliding motility mechanism powered by actomyosin motors and a dynamic ...Apicomplexan parasites, responsible for toxoplasmosis, cryptosporidiosis and malaria, invade host cells through a unique gliding motility mechanism powered by actomyosin motors and a dynamic organelle called the conoid. Here, using cryo-electron microscopy, we determined structures of four essential complexes of the Toxoplasma gondii conoid: the preconoidal P2 ring, tubulin-based conoid fibers, and the subpellicular and intraconoidal microtubules. Our analysis identified 40 distinct conoid proteins, several of which are essential for parasite lytic growth, as revealed through genetic disruption studies. Comparative analysis of the tubulin-containing complexes sheds light on their functional specialization by microtubule-associated proteins, while the structure of the preconoidal ring pinpoints the site of actin polymerization and initial translocation, enhancing our mechanistic understanding of gliding motility and, therefore, parasite invasion.
External linksNat Struct Mol Biol / PubMed:41366525
MethodsEM (single particle)
Resolution3.14 - 3.54 Å
Structure data

72715

9y9z

EMDB-72715, PDB-9y9z:
Cryo-EM structure of conoid fiber from Toxoplasma gondii (24-nm repeat)
Method: EM (single particle) / Resolution: 3.14 Å

72716

9ya0

EMDB-72716, PDB-9ya0:
Cryo-EM structure of pre-conoid ring 2 (PCR P2 ring)from Toxoplasma gondii
Method: EM (single particle) / Resolution: 3.54 Å

72717

9ya1

EMDB-72717, PDB-9ya1:
Cryo-EM structure of intraconoidal microtubule 2 (ICMT2) from Toxoplasma gondii (8-nm repeat)
Method: EM (single particle) / Resolution: 3.52 Å

72718

9ya2

EMDB-72718, PDB-9ya2:
Cryo-EM structure of intraconoidal microtubule 1 (ICMT1) from Toxoplasma gondii (8-nm repeat)
Method: EM (single particle) / Resolution: 3.34 Å

72719

9ya3

EMDB-72719, PDB-9ya3:
Cryo-EM structure of the apical region of subpellicular microtubule (SPMT) from Toxoplasma gondii (8-nm repeat)
Method: EM (single particle) / Resolution: 3.3 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • toxoplasma gondii (eukaryote)
KeywordsSTRUCTURAL PROTEIN / Tubulin / Microtubule / Microtubule Inner Protein / Microtubule-associated Protein / Toxoplasma gondii / conoid / conoid fiber / pre-conoid ring / PCR / P2 / intraconoidal microtubule / ICMT / subpellicular microtubule / SPMT

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