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- PDB-9y9z: Cryo-EM structure of conoid fiber from Toxoplasma gondii (24-nm r... -

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Basic information

Entry
Database: PDB / ID: 9y9z
TitleCryo-EM structure of conoid fiber from Toxoplasma gondii (24-nm repeat)
Components
  • CF1, TGME49_222350
  • CF2, TGME49_258090
  • CF3, TGME49_255895
  • CF4, TGME49_246720
  • CF5, TGME49_297180
  • CF6, TGME49_245640
  • CIP3, TGME49_225020
  • CPH1, TGME49_266630
  • DCX, TGME49_256030
  • Microtubule associated protein SPM1
  • PRP, TGME49_291880
  • Spindle assembly abnormal protein 6 N-terminal domain-containing protein
  • Tubulin alpha chain
  • Tubulin beta chain
KeywordsSTRUCTURAL PROTEIN / Tubulin / Microtubule / Microtubule Inner Protein / Microtubule-associated Protein / Toxoplasma gondii / conoid / conoid fiber
Function / homology
Function and homology information


condensin complex / mitotic chromosome condensation / microtubule polymerization / microtubule-based process / condensed chromosome / tubulin binding / structural constituent of cytoskeleton / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule ...condensin complex / mitotic chromosome condensation / microtubule polymerization / microtubule-based process / condensed chromosome / tubulin binding / structural constituent of cytoskeleton / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cytoskeleton / intracellular signal transduction / hydrolase activity / GTPase activity / chromatin binding / chromatin / GTP binding / metal ion binding / cytoplasm
Similarity search - Function
P25-alpha / p25-alpha / Stabilizer of axonemal microtubules 1/2 / Spindle assembly abnormal protein 6, N-terminal / SAS-6, N-terminal domain superfamily / Doublecortin / Centriolar protein SAS N-terminal domain / Doublecortin domain profile. / Domain in the Doublecortin (DCX) gene product / Doublecortin domain ...P25-alpha / p25-alpha / Stabilizer of axonemal microtubules 1/2 / Spindle assembly abnormal protein 6, N-terminal / SAS-6, N-terminal domain superfamily / Doublecortin / Centriolar protein SAS N-terminal domain / Doublecortin domain profile. / Domain in the Doublecortin (DCX) gene product / Doublecortin domain / Doublecortin domain superfamily / CRAL-TRIO lipid binding domain superfamily / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Ankyrin repeat-containing domain superfamily
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / EF hand protein / Uncharacterized protein / Uncharacterized protein / Microtubule associated protein SPM1 / Ankyrin repeat-containing protein / Spindle assembly abnormal protein 6 N-terminal domain-containing protein / Doublecortin domain-containing protein / Tubulin beta chain ...GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / EF hand protein / Uncharacterized protein / Uncharacterized protein / Microtubule associated protein SPM1 / Ankyrin repeat-containing protein / Spindle assembly abnormal protein 6 N-terminal domain-containing protein / Doublecortin domain-containing protein / Tubulin beta chain / Tubulin alpha chain / Bax inhibitor 1, related protein / CRAL/TRIO domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein
Similarity search - Component
Biological speciesToxoplasma gondii (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.14 Å
AuthorsZeng, J. / Zhang, R.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI179885 United States
CitationJournal: To Be Published
Title: Atomic models of the conoid, the cell invasion machinery of the human parasite Toxoplasma gondii
Authors: Zeng, J. / Fu, Y. / Qian, P. / Sibley, L.D. / Zhang, R.
History
DepositionSep 15, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 3, 2025Provider: repository / Type: Initial release
Revision 1.0Dec 3, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
Revision 1.0Dec 3, 2025Data content type: FSC / Data content type: FSC / Provider: repository / Type: Initial release
Revision 1.0Dec 3, 2025Data content type: Half map / Part number: 1 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Dec 3, 2025Data content type: Half map / Part number: 2 / Data content type: Half map / Provider: repository / Type: Initial release
Revision 1.0Dec 3, 2025Data content type: Image / Data content type: Image / Provider: repository / Type: Initial release
Revision 1.0Dec 3, 2025Data content type: Mask / Part number: 1 / Data content type: Mask / Provider: repository / Type: Initial release
Revision 1.0Dec 3, 2025Data content type: Primary map / Data content type: Primary map / Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: CF2, TGME49_258090
B: CF2, TGME49_258090
C: CF2, TGME49_258090
D: CF2, TGME49_258090
E: CF2, TGME49_258090
F: CF2, TGME49_258090
G: CF2, TGME49_258090
H: CF2, TGME49_258090
I: CF2, TGME49_258090
J: CF2, TGME49_258090
K: CF2, TGME49_258090
L: CF2, TGME49_258090
M: CF2, TGME49_258090
N: CF2, TGME49_258090
O: CF2, TGME49_258090
P: CF2, TGME49_258090
Q: CF2, TGME49_258090
R: CF2, TGME49_258090
S: CF2, TGME49_258090
T: CF2, TGME49_258090
U: CF2, TGME49_258090
V: CF2, TGME49_258090
W: CF2, TGME49_258090
X: CF2, TGME49_258090
Y: CF2, TGME49_258090
Z: CF2, TGME49_258090
1: CF2, TGME49_258090
10: CF1, TGME49_222350
11: CF1, TGME49_222350
12: CF1, TGME49_222350
13: CF1, TGME49_222350
14: CF1, TGME49_222350
15: CF1, TGME49_222350
16: CF1, TGME49_222350
1A: Tubulin alpha chain
1B: Tubulin beta chain
1C: Tubulin alpha chain
1D: Tubulin beta chain
1E: Tubulin alpha chain
1F: Tubulin beta chain
1G: Tubulin alpha chain
1H: Tubulin beta chain
1I: Tubulin alpha chain
1J: Tubulin beta chain
1K: Tubulin alpha chain
1L: Tubulin beta chain
1M: Tubulin alpha chain
1N: Tubulin beta chain
2: CF2, TGME49_258090
20: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
21: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
22: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
23: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
24: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
25: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
26: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
27: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
28: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
29: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
2A: Tubulin alpha chain
2B: Tubulin beta chain
2C: Tubulin alpha chain
2D: Tubulin beta chain
2E: Tubulin alpha chain
2F: Tubulin beta chain
2G: Tubulin alpha chain
2H: Tubulin beta chain
2I: Tubulin alpha chain
2J: Tubulin beta chain
2K: Tubulin alpha chain
2L: Tubulin beta chain
2M: Tubulin alpha chain
3: CF1, TGME49_222350
30: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
31: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
32: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
33: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
35: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
36: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
37: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
38: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
39: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
3A: Tubulin alpha chain
3B: Tubulin beta chain
3C: Tubulin alpha chain
3D: Tubulin beta chain
3E: Tubulin alpha chain
3F: Tubulin beta chain
3G: Tubulin alpha chain
3H: Tubulin beta chain
3I: Tubulin alpha chain
3J: Tubulin beta chain
3K: Tubulin alpha chain
3L: Tubulin beta chain
3M: Tubulin alpha chain
4: CF1, TGME49_222350
40: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
42: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
43: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
44: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
45: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
46: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
47: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
48: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
49: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
4A: Tubulin alpha chain
4B: Tubulin beta chain
4C: Tubulin alpha chain
4D: Tubulin beta chain
4E: Tubulin alpha chain
4F: Tubulin beta chain
4G: Tubulin alpha chain
4H: Tubulin beta chain
4I: Tubulin alpha chain
4J: Tubulin beta chain
4K: Tubulin alpha chain
4L: Tubulin beta chain
4M: Tubulin alpha chain
5: CF1, TGME49_222350
50: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
51: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
52: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
53: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
54: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
55: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
56: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
57: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
58: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
59: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
5A: Tubulin alpha chain
5B: Tubulin beta chain
5C: Tubulin alpha chain
5D: Tubulin beta chain
5E: Tubulin alpha chain
5F: Tubulin beta chain
5G: Tubulin alpha chain
5H: Tubulin beta chain
5I: Tubulin alpha chain
5J: Tubulin beta chain
5K: Tubulin alpha chain
5L: Tubulin beta chain
5M: Tubulin alpha chain
5N: Tubulin beta chain
6: CF1, TGME49_222350
60: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
61: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
62: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
63: CIP3, TGME49_225020
64: CIP3, TGME49_225020
65: CIP3, TGME49_225020
66: CIP3, TGME49_225020
67: CIP3, TGME49_225020
68: CIP3, TGME49_225020
69: CIP3, TGME49_225020
6A: Tubulin alpha chain
6B: Tubulin beta chain
6C: Tubulin alpha chain
6D: Tubulin beta chain
6E: Tubulin alpha chain
6F: Tubulin beta chain
6G: Tubulin alpha chain
6H: Tubulin beta chain
6I: Tubulin alpha chain
6J: Tubulin beta chain
6K: Tubulin alpha chain
6L: Tubulin beta chain
6M: Tubulin alpha chain
6N: Tubulin beta chain
7: CF1, TGME49_222350
71: CF6, TGME49_245640
72: CF6, TGME49_245640
73: CF6, TGME49_245640
75: CF6, TGME49_245640
76: CF6, TGME49_245640
77: CF6, TGME49_245640
78: CF6, TGME49_245640
79: CF6, TGME49_245640
7A: Tubulin alpha chain
7B: Tubulin beta chain
7C: Tubulin alpha chain
7D: Tubulin beta chain
7E: Tubulin alpha chain
7F: Tubulin beta chain
7G: Tubulin alpha chain
7H: Tubulin beta chain
7I: Tubulin alpha chain
7J: Tubulin beta chain
7K: Tubulin alpha chain
7L: Tubulin beta chain
7M: Tubulin alpha chain
8: CF1, TGME49_222350
80: CF6, TGME49_245640
82: CF6, TGME49_245640
83: CF6, TGME49_245640
84: CF6, TGME49_245640
85: CF6, TGME49_245640
86: CF6, TGME49_245640
87: CF6, TGME49_245640
89: CF6, TGME49_245640
8A: Tubulin alpha chain
8B: Tubulin beta chain
8C: Tubulin alpha chain
8D: Tubulin beta chain
8E: Tubulin alpha chain
8F: Tubulin beta chain
8G: Tubulin alpha chain
8H: Tubulin beta chain
8I: Tubulin alpha chain
8J: Tubulin beta chain
8K: Tubulin alpha chain
8L: Tubulin beta chain
8M: Tubulin alpha chain
9: CF1, TGME49_222350
90: CF6, TGME49_245640
91: CF6, TGME49_245640
92: CF6, TGME49_245640
93: CF6, TGME49_245640
94: CF6, TGME49_245640
95: CF6, TGME49_245640
96: CF6, TGME49_245640
97: CF6, TGME49_245640
9A: Tubulin alpha chain
9B: Tubulin beta chain
9C: Tubulin alpha chain
9D: Tubulin beta chain
9E: Tubulin alpha chain
9F: Tubulin beta chain
9G: Tubulin alpha chain
9H: Tubulin beta chain
9I: Tubulin alpha chain
9J: Tubulin beta chain
9K: Tubulin alpha chain
9L: Tubulin beta chain
9M: Tubulin alpha chain
A1: CF4, TGME49_246720
A2: CF4, TGME49_246720
A3: CF4, TGME49_246720
A4: CF4, TGME49_246720
A5: CF4, TGME49_246720
A6: CF4, TGME49_246720
A7: CF4, TGME49_246720
A8: CF4, TGME49_246720
A9: CF4, TGME49_246720
B1: CF3, TGME49_255895
B2: CF3, TGME49_255895
B3: CF3, TGME49_255895
B4: CF3, TGME49_255895
B5: CF3, TGME49_255895
B6: CF3, TGME49_255895
B7: CF3, TGME49_255895
C0: CPH1, TGME49_266630
C1: Microtubule associated protein SPM1
C2: Microtubule associated protein SPM1
C3: Microtubule associated protein SPM1
C4: Microtubule associated protein SPM1
C5: Microtubule associated protein SPM1
C6: CPH1, TGME49_266630
C7: CPH1, TGME49_266630
C8: CPH1, TGME49_266630
C9: CPH1, TGME49_266630
D0: CPH1, TGME49_266630
D1: CPH1, TGME49_266630
D2: CPH1, TGME49_266630
D3: CPH1, TGME49_266630
D4: CPH1, TGME49_266630
D5: CPH1, TGME49_266630
D6: CPH1, TGME49_266630
D7: CPH1, TGME49_266630
D8: CPH1, TGME49_266630
D9: CPH1, TGME49_266630
E0: CPH1, TGME49_266630
E1: CPH1, TGME49_266630
E2: CPH1, TGME49_266630
E3: CPH1, TGME49_266630
E4: CPH1, TGME49_266630
E5: CPH1, TGME49_266630
E6: CPH1, TGME49_266630
E7: CPH1, TGME49_266630
E8: CPH1, TGME49_266630
E9: CPH1, TGME49_266630
F0: PRP, TGME49_291880
F1: CPH1, TGME49_266630
F2: CPH1, TGME49_266630
F3: CPH1, TGME49_266630
F5: PRP, TGME49_291880
F6: PRP, TGME49_291880
F7: PRP, TGME49_291880
F8: PRP, TGME49_291880
F9: PRP, TGME49_291880
G0: PRP, TGME49_291880
G1: PRP, TGME49_291880
G2: PRP, TGME49_291880
G3: PRP, TGME49_291880
G4: PRP, TGME49_291880
G5: PRP, TGME49_291880
G6: PRP, TGME49_291880
G7: PRP, TGME49_291880
G8: PRP, TGME49_291880
G9: PRP, TGME49_291880
H0: PRP, TGME49_291880
H1: PRP, TGME49_291880
H2: PRP, TGME49_291880
H3: PRP, TGME49_291880
H4: PRP, TGME49_291880
H5: PRP, TGME49_291880
H6: PRP, TGME49_291880
H7: PRP, TGME49_291880
H8: PRP, TGME49_291880
H9: PRP, TGME49_291880
I1: PRP, TGME49_291880
I2: PRP, TGME49_291880
I3: PRP, TGME49_291880
I4: PRP, TGME49_291880
I5: PRP, TGME49_291880
I6: PRP, TGME49_291880
I7: PRP, TGME49_291880
I8: PRP, TGME49_291880
I9: PRP, TGME49_291880
J1: CF5, TGME49_297180
J2: CF5, TGME49_297180
J3: CF5, TGME49_297180
J4: CF5, TGME49_297180
J5: CF5, TGME49_297180
J6: CF5, TGME49_297180
J7: CF5, TGME49_297180
K0: DCX, TGME49_256030
K1: DCX, TGME49_256030
K2: DCX, TGME49_256030
K3: DCX, TGME49_256030
K4: DCX, TGME49_256030
K5: DCX, TGME49_256030
K6: DCX, TGME49_256030
K7: DCX, TGME49_256030
K8: DCX, TGME49_256030
K9: DCX, TGME49_256030
L0: DCX, TGME49_256030
L1: DCX, TGME49_256030
L2: DCX, TGME49_256030
L3: DCX, TGME49_256030
L4: DCX, TGME49_256030
L5: DCX, TGME49_256030
L6: DCX, TGME49_256030
L7: DCX, TGME49_256030
L8: DCX, TGME49_256030
L9: DCX, TGME49_256030
M1: DCX, TGME49_256030
M2: DCX, TGME49_256030
M3: DCX, TGME49_256030
M4: DCX, TGME49_256030
M5: DCX, TGME49_256030
M6: DCX, TGME49_256030
M7: DCX, TGME49_256030
a: CF2, TGME49_258090
b: CF2, TGME49_258090
c: CF2, TGME49_258090
d: CF2, TGME49_258090
e: CF2, TGME49_258090
f: CF2, TGME49_258090
g: CF2, TGME49_258090
h: CF2, TGME49_258090
i: CF2, TGME49_258090
j: CF2, TGME49_258090
k: CF2, TGME49_258090
l: CF2, TGME49_258090
m: CF2, TGME49_258090
n: CF2, TGME49_258090
o: CF2, TGME49_258090
p: CF2, TGME49_258090
q: CF2, TGME49_258090
r: CF2, TGME49_258090
s: CF2, TGME49_258090
t: CF2, TGME49_258090
u: CF2, TGME49_258090
v: CF2, TGME49_258090
w: CF2, TGME49_258090
x: CF2, TGME49_258090
y: CF2, TGME49_258090
z: CF2, TGME49_258090
hetero molecules


Theoretical massNumber of molelcules
Total (without water)22,934,895552
Polymers22,875,360378
Non-polymers59,535174
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Protein , 14 types, 378 molecules ABCDEFGHIJKLMNOPQRSTUVWXYZ12ab...

#1: Protein ...
CF2, TGME49_258090


Mass: 106251.133 Da / Num. of mol.: 54 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8GBZ2
#2: Protein
CF1, TGME49_222350


Mass: 47976.344 Da / Num. of mol.: 14 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8GJP0
#3: Protein ...
Tubulin alpha chain / Alpha-tubulin / alpha tubulin TUBA1 / TGME49_316400


Mass: 50166.645 Da / Num. of mol.: 63 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: P10873
#4: Protein ...
Tubulin beta chain / beta tubulin / TGME49_266960


Mass: 50119.121 Da / Num. of mol.: 57 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: I7BFC9
#5: Protein ...
Spindle assembly abnormal protein 6 N-terminal domain-containing protein / SAS6L / TGME49_301420


Mass: 29529.971 Da / Num. of mol.: 41 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A7J6KDX1
#6: Protein
CIP3, TGME49_225020


Mass: 114592.602 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8G5Q1
#7: Protein ...
CF6, TGME49_245640


Mass: 46600.648 Da / Num. of mol.: 24 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A086KRX4
#8: Protein
CF4, TGME49_246720 / EF hand protein


Mass: 56022.809 Da / Num. of mol.: 9 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A086KR31
#9: Protein
CF3, TGME49_255895 / Bax inhibitor 1 / related protein


Mass: 62988.641 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8F3E8
#10: Protein ...
CPH1, TGME49_266630 / Ankyrin repeat-containing protein


Mass: 67410.492 Da / Num. of mol.: 28 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A7J6KB56
#11: Protein
Microtubule associated protein SPM1 / SPM1 / TGME49_263520


Mass: 38845.750 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A7J6K285
#12: Protein ...
PRP, TGME49_291880


Mass: 80452.414 Da / Num. of mol.: 35 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A151HK62
#13: Protein
CF5, TGME49_297180 / CRAL/TRIO domain-containing protein


Mass: 97829.719 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8FEB8
#14: Protein ...
DCX, TGME49_256030 / Doublecortin domain-containing protein / TgDCX


Mass: 29261.402 Da / Num. of mol.: 27 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: B6KAS6

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Non-polymers , 3 types, 174 molecules

#15: Chemical...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 63 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Feature type: SUBJECT OF INVESTIGATION / Comment: GTP, energy-carrying molecule*YM
#16: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 54 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#17: Chemical...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 57 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: GDP, energy-carrying molecule*YM

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: conoid fiber from Toxoplasma gondii (24-nm repeat) / Type: COMPLEX / Entity ID: #1-#14 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 25000 nm / Nominal defocus min: 5000 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

EM software
IDNameCategory
1cryoSPARCparticle selection
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.14 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 95409 / Symmetry type: POINT

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