EMDB-72716: Cryo-EM structure of pre-conoid ring 2 (PCR P2 ring)from Toxoplas...

Basic information

Entry

Database: EMDB / ID: EMD-72716

• Title: Cryo-EM structure of pre-conoid ring 2 (PCR P2 ring)from Toxoplasma gondii

Sample

• Complex: conoid fiber from Toxoplasma gondii (24-nm repeat)
  • Protein or peptide: x 20 types

• Keywords: Tubulin / Microtubule / Microtubule Inner Protein / Microtubule-associated Protein / Toxoplasma gondii / conoid / conoid fiber / pre-conoid ring / PCR / P2 / STRUCTURAL PROTEIN

Function / homology

Function:

cGMP-dependent protein kinase / cGMP-dependent protein kinase activity / COPII-coated vesicle cargo loading / COPII vesicle coat / myosin complex / cAMP-dependent protein kinase inhibitor activity / myosin II complex / cAMP-dependent protein kinase complex / microfilament motor activity / protein kinase A catalytic subunit binding / endoplasmic reticulum exit site / cAMP binding / GTPase activator activity / actin filament organization / SNARE binding / methyltransferase activity / intracellular protein transport / actin filament binding / actin cytoskeleton organization / methylation / Golgi membrane / calcium ion binding / endoplasmic reticulum membrane / zinc ion binding / ATP binding / membrane / cytoplasm / cytosol

Domain/homology:

CLU domain / CLU domain containing protein / CLU central domain / : / Translation initiation factor eIF3 subunit 135 / Clueless (Clu) domain profile. / : / : / Protein transport protein Sec23 / : / Zinc finger, Sec23/Sec24-type / Sec23/Sec24, trunk domain / Sec23/Sec24, helical domain / Sec23/Sec24 beta-sandwich / Zinc finger, Sec23/Sec24-type superfamily / Sec23/Sec24 helical domain superfamily / Sec23/Sec24 zinc finger / Sec23/Sec24 trunk domain / Sec23/Sec24 helical domain / Sec23/Sec24 beta-sandwich domain / Formin, FH2 domain / Formin, FH2 domain superfamily / Formin Homology 2 Domain / Formin homology-2 (FH2) domain profile. / Formin Homology 2 Domain / Regulator of chromosome condensation (RCC1) repeat / Regulator of chromosome condensation, RCC1 / Regulator of chromosome condensation (RCC1) repeat profile. / : / Filamin family / Gelsolin-like domain superfamily / Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II / Filamin/ABP280 repeat / Tetratricopeptide repeat / Filamin-type immunoglobulin domains / Filamin/ABP280 repeat / Ca2+ insensitive EF hand / Filamin/ABP280 repeat profile. / Filamin/ABP280 repeat-like / EF hand / Gelsolin-like domain / Gelsolin repeat / Ankyrin repeats (many copies) / ADF-H/Gelsolin-like domain superfamily / B-box zinc finger / Cyclic nucleotide-binding domain signature 2. / B-Box-type zinc finger / B-box-type zinc finger / Zinc finger B-box type profile. / EF-hand domain pair / Cyclic nucleotide-binding domain signature 1. / Cyclic nucleotide-binding, conserved site / Short calmodulin-binding motif containing conserved Ile and Gln residues. / IQ motif, EF-hand binding site / Myosin head, motor domain / Myosin head (motor domain) / Myosin. Large ATPases. / IQ motif profile. / SET domain superfamily / Cyclic nucleotide-monophosphate binding domain / Cyclic nucleotide-binding domain / cAMP/cGMP binding motif profile. / Cyclic nucleotide-binding domain / Cyclic nucleotide-binding domain superfamily / Kinesin motor domain superfamily / : / PH domain profile. / von Willebrand factor A-like domain superfamily / Pleckstrin homology domain. / SKP1/BTB/POZ domain superfamily / Pleckstrin homology domain / RmlC-like jelly roll fold / EF-hand domain pair / Ankyrin repeats (3 copies) / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Leucine-rich repeat domain superfamily / EF-hand, calcium binding motif / Ankyrin repeat-containing domain superfamily / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair / Tetratricopeptide-like helical domain superfamily / Immunoglobulin E-set / Immunoglobulin-like fold / P-loop containing nucleoside triphosphate hydrolase

Component:

GYF domain-containing protein / Protein transport protein SEC23 / B-box zinc finger domain-containing protein / Uncharacterized protein / Calmodulin / Putative transport protein Sec24 / Calmodulin / Zinc finger protein / Putative myosin light chain MLC4 / Formin 1 / Cyclic nucleotide-binding domain-containing protein / Histone lysine methyltransferase, SET, putative / Filamin/ABP280 repeat-containing protein / Myosin head (Motor domain) domain-containing protein / Clu domain-containing protein / Leucine rich repeat-containing protein / CLU central domain-containing protein / PH domain-containing protein / Uncharacterized protein / Filamin/ABP280 repeat-containing protein

• Biological species: Toxoplasma gondii (eukaryote)
• Method: single particle reconstruction / cryo EM / Resolution: 3.54 Å
• Authors: Zeng J / Zhang R

Funding support

United States, 1 items

Organization	Grant number	Country
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)	R01AI179885	United States

• Citation: Journal: To Be Published; Title: Atomic models of the conoid, the cell invasion machinery of the human parasite Toxoplasma gondii; Authors: Zeng J / Fu Y / Qian P / Sibley LD / Zhang R

History

Deposition	Sep 15, 2025	-
Header (metadata) release	Nov 19, 2025	-
Map release	Nov 19, 2025	-
Update	Nov 19, 2025	-
Current status	Nov 19, 2025	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_72716.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Voxel size: X=Y=Z: 1.34 Å

Density

Contour Level	By AUTHOR: 0.02
Minimum - Maximum	0.0 - 2.0732958
Average (Standard dev.)	0.003453252 (±0.038748074)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order X Y Z Origin 0 0 0 Dimensions 512 512 512 Spacing 512 512 512
Cell	A=B=C: 686.08 Å α=β=γ: 90.0 °

Supplemental data

Additional map: #1

• File: emd_72716_additional_1.map

Half map: #2

• File: emd_72716_half_map_1.map

Half map: #1

• File: emd_72716_half_map_2.map

Sample components

Entire : conoid fiber from Toxoplasma gondii (24-nm repeat)

• Entire: Name: conoid fiber from Toxoplasma gondii (24-nm repeat)

Components

• Complex: conoid fiber from Toxoplasma gondii (24-nm repeat)
  • Protein or peptide: PCR5, TGME49_242320
  • Protein or peptide: PCR4, TGME49_201220
  • Protein or peptide: Formin 1
  • Protein or peptide: PCR11, TGME49_209490
  • Protein or peptide: CGP, TGME49_240380
  • Protein or peptide: FLM1, TGME49_271780
  • Protein or peptide: Transport protein Sec24
  • Protein or peptide: Protein transport protein SEC23
  • Protein or peptide: FLM2, TGME49_285990
  • Protein or peptide: PCR12, TGME49_292170
  • Protein or peptide: PCR10, TGME49_298010
  • Protein or peptide: PCR14, TGME49_311880
  • Protein or peptide: ICAP16, TGME49_202120
  • Protein or peptide: PCR12, TGME49_219070
  • Protein or peptide: myosin L, TGME49_291020
  • Protein or peptide: PCR13, TGME49_284620
  • Protein or peptide: PCR15, TGME49_232560
  • Protein or peptide: MLC4, TGME49_294390
  • Protein or peptide: Calmodulin
  • Protein or peptide: Calmodulin

Supramolecule #1: conoid fiber from Toxoplasma gondii (24-nm repeat)

• Supramolecule: Name: conoid fiber from Toxoplasma gondii (24-nm repeat) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
• Source (natural): Organism: Toxoplasma gondii (eukaryote)

Macromolecule #1: PCR5, TGME49_242320

• Macromolecule: Name: PCR5, TGME49_242320 / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 119.326812 KDa

Sequence

String:

MATAPPTVAP PRGWTAGAVS SFSEAAHPTE DRPSLVNVSV IEDLQILAQW EYWIQLSLFT ADLHIVGGWN LATPAQQQLF DAKASALKG PIVYSFLDLN ELETPQSLEN IIAEKRVKSD WRKQIFPSAE CRPPFGFEGN PSGTFHVMVC KLALGSVLAK D SEPGETGD EDFKSCDIPG NFTALSRRDE CGGNRGGTNN ESNGAYRVLY RLKRADQVLP TAVLEFEMKP LRIQVRQPVC EM CEVLPAT LYCPADRAQL CDTCDERVHS ATRMLARHVR VPANHSPLQF GSCPYHPSEM IDSVCMTCFV ALCPHCILIG SHS AADFSE HPLISTLDAY KMVMQDSSPS EEALLERRRR IDRELVEKHR QLGQLHSNFL SVQKRIDEAS KNLVGQLSAM KERK TNYLL AVKRELLSEM LVIEWMETFF AHLRLSLSPA EFLSYKHKFD LAAANIFGGA VRVDVDLDSM PAWMSEKFIL QGSFS VTTA PYAVPSLKTL DVPADQQSMN VFLTRLGSRT RTDKMKRSDG KDEDATGPLA LLDKEGESVP EAKWVGLPSE VEGLDE GRV CGHTKMPCFP EYARDQVSIA EEGPIASAVE YIPTSAEAAT DPNALPPQTR EIVARAREAE ERGENLPTLM SILSPLQ KV PKAVCRRLWN LLSDNNFIPL LTLLKASDGD QRRRLVAAAC NLANSFSAVP DLAYHLFRYE LKWVGHLPAS LFLHSSSL L FPFLSFVTSS KEMLLPGDLS WLHQVMDPVL AAADSIVASE HLSADQILRE TPAKLFRPAG SLFLERLKRL SLADFPCTF RGLLYLLADA VDAQLRESPV TSFASAHHVT SSAIATCAET LICCFFGNFI LRYAPKHVVQ DARDGLGRAT SDFSADERTD LVGLRRMRE IAERSRVPPT YGAMDKLLRQ MASFCWEAVS SLPRRVLRPG SERVAGSETR EERENGDRED GRRSRPDSDA L ATEAALRN VEEQFLAQAG KEIVDWCALQ LKLPRLESPL KFSSAVLRKA ADEATGEFLQ YILRLDRDMR EGTFQSGTPG LT ERDVATP AYEELLEFAI RASTYEDPAS EALGWR

UniProtKB: B-box zinc finger domain-containing protein

Macromolecule #2: PCR4, TGME49_201220

• Macromolecule: Name: PCR4, TGME49_201220 / type: protein_or_peptide / ID: 2 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 68.040531 KDa

Sequence

String:

MATSVGGDVR LSEKDLGAVL RDSDEWAFLE YLLQMSVRTS RVRLLQAWHI ATPHLVSAFE RRTQGRLLTY SFVDTASLDE DNSLQDIAR RGLKIPMTGM RFAVGNFSLP GFPLMRGGPA EAEDPFVLPS SAVSTRQEEA KKDRSKFLDA ARSQLLTGER R IFEYLLCQ VGVGRSVVVE DEKEACGSRF SLLWEYDSAY LEKGALAPEE SLTVHYQAVD DSKSGDDGPL KKTKDQEEAR NS RPASLPS IFGDGKKVTP GVLPQYTFRH DYVVYDSSQV LPRFFLLFEM DPSLEELFAV PLCDNCQDNP ACIWCPADAA RLC ASCDEL IHQQNRLVSR HIRVPLNEMP RAFGTCRRHP GEVYELFCSI CHVAVCRLCR GNHLHAAPSK ALGDEGSLSI VAGG RSLMP LNKPYQAVLH MAKKPHFVLA ARRTELEKRL EEVQKMLDGV RRNCREEEQR CYAILEEAIT QLHSCTEDKM GAVLS RQLE LQREIDTMDW SESFLQYLRT VLPPADFLHA WLRHCRLRDE LDALAGDPSM LSRNVFPDMR LQGRVDILTD SALRRR DYV AVEGRRAPMA TAGSDKKGPD EQRRKSPEKR VHFGSTLLAG

UniProtKB: Zinc finger protein

Macromolecule #3: Formin 1

• Macromolecule: Name: Formin 1 / type: protein_or_peptide / ID: 3 / Number of copies: 12 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 552.6045 KDa

Sequence

String:

MSCPASCPRG RGGSAPLPPM PPSVNDDRNA VLNALPSRTS FSAPLCSATS CSLPERPVFA KLTGGMPFSR TTARIECASP ACHESLSLH RVAESQVKAQ AAASGPLSNS SFLIFLNSIP TEPPRCFLPP RRVASLSAEA DTPKGSFYVF SESSRSESSH S EGCVESRL SGHTAARTRC RRSRTTASAT DSRRDDLFSF DLPQECGSAP RLPLPLPSRS CPASPCHSLL PPLSNKLTPP CR ESQSEGT DHGSSSARCC QPRPAYPPRI TDALPTGQIY SRSPSRVRER TLRSEPAAPV NYSDTQKRCP APLFQSIPVH SMP PPPPPP KGKKGPLPKK ASNSPLPPKG KSTPDEGAKK TPPPPPAAKA GVPEGKAEMP SEKAEVPQKK AGVPSEKADV PQKK AGVPK EKAGMPSEKA EVPQKKAGLP KEKAGIPPEK AGVPQEKTGL PKEKAGIPQE KAGISKGESG SPEGKTGPLP GKAGP PKGK LPPGKAPPAE GSGPPAKSDL KASPAGAPPS SLPSKKAVAS AGKPLGGKTP PAPSVAPNPP LGSSGPQESG SSTYYR DTL RAAAGTAGGI ECSAGEGSGE TVPPPNEAYA AKAAMMVESL KLADYTNVPA AHAHGTEPPA LPFAPGADRN PAILTRA LK KEMELRRGQP TQTPEERRLM LAGTFSLHQA RLGFKLSPEH QRVAEALMLL RRLEDFRGSV QAGEVQRIRY LLRVGATE A FSQQRYSDAM LQALHSYLLA KSYYTAYQSA PLEGEMVPEL LLLVKCCGLA GCPDRGQEYL KELRYIVENT LIAEAGGSA AGNRGSLVGP IMRVEKHVLA SVVSSLAELC SLYNQPDDAR LFYEKYVILT KDVYGADSLA VSDALNTVAL YFFRTRQYGQ ALPLVEQVL AIRKKHLGDF DAAEPHARVA DCYANIGLLH RLLGHPTEAF PCVLIALDMR MRIYQTRETS GVQDLVLALG C LQHQAGHF RAALEAYKEV WTFRNNTLGV THPDTVNVEL LLRQLEKDLA AQMQEDKQRF VQRDKALPSA SAFDLTTRAS SQ ISVSRQS HRPGDFFYEE ADGAASSDRG RRGSLDFADA SATVRLLKPA AAATLEAYRQ RHAACGWTQP LREKSHFHSV ARV RALYGE EWRSLMVPSA AILPYVDVEV TGPDTLKLAE CFQFQEALAV DRPTLPVVNV PQIERGEAVL EQGHPHMVLS PDAA ELLSQ WGVALPEVDE KGQVINREAK LEKHGRLFLP LVDPSTNQPF LGFYGEPVMV PNPAVYHSVI APAILAYARR QAALV DRAK NAVGPAQKQR SAMVQGAKQV ARGVVMAKLG AKGKAGAKTP AQDAPKGLET KDAPATAAPA AKEGGAPETA ENAPPK VEA AAAAPDKEES VEKKEEASPS LPRKVSELPA KKSLLRSKAS QEIPKAPPLK DDSSAPESGS DSGASDRKRS PSLSTKK SF AKKTSAALPT PAPEGATADG KPKSLLGSGA KKFDPSAYKA KVKAGPAPGK ALSFRATPKH AAALPPSNHL REPATLSD P DAILERKQKS RTKAQTTAAD SHELGRNDML EREEEGDGGG EEGLAKRKKV VFQKNVEKGR KVSELQHEAS RSADGRVGK NQQGPAAGRH GLKAKKLNAE TERNSESEEK SSCGFAAPQS SCTRKREGSP ETRCINVESV PQPQEGPDGS RDTTLASLSP PDSSSSLRC KKASSKLELS VECSASRDEP SEAEDRREEN RREERREKTR RDFKALLLKK QREHMSETLM RLQKETDREA S ESASECKI FLKMQRSLER KDEVGLFFPR ASGNQTTPPA FQRASWQAAA LAGHLRSGDS LWTRGEETAE ADIMGRGDES AL LTRRVEE ERKRVRERLL GCDTRASHRE TSLGADSGLF ALDREAQRPA ENREFPEPHC VSSSPRKAID QVPDLNCRAA LDP ERKSFS PDSDRETSIA LCRRRVSPSG PVSSPDSRWR GDRRRNASSR PTLDWCLSQQ GNATESSSPH RDTTSRAPDS PHVK AASKE SNFLSMPFLA AAAPPPPGLF SPWASPVNSD GNGDTEGTLW DEGIEAGGSS LTRRTRSSER SLEKRSVRQK APSQV SEAR GTLMSSLNAG LEAKKTRADE RRDIGTVFCL SDSRDTEVKP GEETLFQSAA GESWGVDRRY VEAEREAEQR RRRRTG GWG RHEGTANPGG RGRTGSRGEA STWRRESTSS ATEAEIRRER VEQSRPDSGR RRDTRERKRS VAQGAVNRVS RATGRTE GV AVLGQERRRC VDARRRNEAE DREGEDLGEA ELSVSCFDSD GDQEKDFRRE TSSEEDNEED GEEAELFSTN EVETDTLR S GRRKQHSVSS RSASPSPWRV GADRTRMKTN SQASAALSGM RTSRFLQPPA FDSSASRSSS ASPSRRRRLE TLETLDLQK RTRKQRVRVG SRESREGNAQ RSPGNRRRDK KGQTEGRRGD WGRAYLERLV VGQETETDSE IATLDEELDR TELPRVVSPQ TFHEAPNPF SDQDTETDCL FSTPRLPVSN ADPQASKTHS EADEYVVSFA SQSSAARSSS CYPSRSPSCS ALSLSFACSD S TVGAESAT GRERTVHLLR PTSSSVSSIS PTSSDCGRLD SELDALPGLG KCTLFFSAPE AVHARPLASP SFRQRLFSRR DS GAERASC GDAFDSWSPT SAGAGSRVVE SCGSNVSSPS RGRLRSRVSL GGLKAAFSRH SENSSNERLC SPMHQETSRK GPL AVARRL SGVASRLVHS RSSLQELGEA EDDSRKRSSE GDSQEIPRDS VGVTALTGDS ETPNGWAGVG GLEVSAGEDS RELR ECEEK ERERTPLGGS LRRFSFSSDL RKDPRRPRRT LSDATATCEG DGKMRRLVAL LRRKNRRVKK EGEQESERER RDEGE QESE RERRDEGEHE GEQESERERR DEREQGGEQE SENEQEAEHE SERERRDERE QGGEQESERE RRDEREQGGE QESERE RRD EREQGGEQES ENEQEAEQEN EREQAVGEQG QEREGEGEQR SECEQGGSVI NVWRQAEKEE SSGKQLSKGE ESREGEK GG VRAADNQASG ATQGEEQEQS GSSRFSQLLE KWARMSGGAT PKQRIEMISD KSEPGGRWRK DMGILKDSEE ESLIGKEG I SQRLTDADTG TEKTREAQRE DAWVVTESAR DRRRALGTTV SLEEVSLCAV GSSESHLEKL GSGGDENDCG RDASEETKQ GHCCEDRRSL KREKEVSDWG VQTSHEREKQ VSARGVETPE TKEELPASPL SGDSSSSPLA GSASNVFPAR KRLFSSRRML RLPIPSRKL RKADKPGSPS DAQNSAVSPS PSFQLSRSAE AATGGPQNVD EREVAVLSGA LEVQTEGWGV QTAPETPGNS T HWASPSFH SLFSRRFSLR DRLRRRSSAS SMTVLDMEEN KSLDALSVDM PATEPQRPLA SVSDSLVFSE GLSFSSCVDP VS DFGDDNE KDGVTQETRS SCSEVPCAGA RAGGRESPTS RRHSFVFRTT QQKYRTEGSA DNRGRRSLKV LRRSSFSRAF RGL VKSPER QILRDRDREE GKAQDTARRS ESEREGEKDD EEKQTGKREG RKLRHLSASF LRIPRPGKHE TNKESVCKRR GASL PPLPP EASSPHGASR LRAAAASFSR LRRRSQSSVN RGKKEAIEIE KMEEETKAQN EIKHERKSEL KQEEVDEMKQ ENVDE IKQE RKGEVKQEEV DEMKQENVDE IKQERKSELK QEEVDEMKQE NVDEIKQERK SELKQEEVDE MKQENVDEIK QETPET VPL WEPSITSHLS DSCGEDVAIF AVEACVRAAR TWQKEDETLG ASVAAGRGED EDECVDRGDA LTGQDDNAEE TSQRCSC SE DSPEGVAERE WMGGSSDGAE IHLLPEADTS DEEDEGEEEE EREEEKEEEG EGEEGEEEEE EGEEEEEEEE EEEEGEEE D IHELGEKEYS SAEEETRIGE AELRHGVAIL NPFASCGAEA YGEERPKTGT TGSEADDDSW AASDWSVGAS SSDEESERS WQEGPSSEED CDETEAEKNA DSEVEELYAE KLCRKKVHAE NDVEECKTEV SKAEEGFQNS MACLAEVLRL TGVPYSESDL SVEIAELNR QSTLLMVTPD TQRLSRELPK SIASPRTSAV PLDKQCDGKT NLTHLPKSAP FRKAMELTRV AYRSGKTPAA C SVLRSSSS SNLASSVSSS RSSSKLVSSS RLYATPREPR SEPREASGGF SVSPIACDSS PFAPPAAPLG ASEGGRESLS CS DSSSVLV GDRPPRFSTQ QLHALPPKGP KGGPPKKGKA TGWKPPPPLP PAAEDGTDVK EGPTVLKQGI FKKAEQAVKK EIP KKEEGS PKEAAKVVEK KVLEKKASVS AAKGETETKV AVKSVAKPPP LKAAKSGEAK TAEPVKEEAK VEKAPKVKGD GFSR MKLPS GKPEEAPKKT PKMKIMVGTT GQAKVVEKKD SMDELDAFTG PCYIPPHMDV PPVAPEVLAK ITKLKPGVEK DPQLG ELPV VILMDDNNTE LARLPWQVDI SSFSLAQSSM SILVARRLGL LARRLDEDKK DEDTRDWLTG EGLKKFGLSM QKEKPT FGD TSLLLGMGLS ALPQALAQKK PEAPKKEEAK PKPEAKKEAP KKGPPGGKGG KGPPGKGPPK GKGKGPKKKG EGGLKVG KK DEGKTKRFFW DPIFEDEIPG TIFMKKPNMV LKLQDVEETF AKVVAKKKTE SKKPKIIQLL PDSKRAYNMS IALSKFNN Y SYQQLREAII DLDPKILTIE ATETLLNFVP TGEENQVVKE YINSGGDLKL VDKPEQFVAA MLGVPLMKQR LEAHAFALN FREAYSDAYT PLENMADACD AIDDSQNLKI VLFAILELGN ALNEGDPQRG GAAGFKPTTL AKLQEIRTTT KPVRTMLQYI CDIIWEQQP TALNIYEDLK ICDKAQRVDM QGIEGRISNL KAGLTKVKNT LEAAKKGNES TGVMGDRDPL RNIMDEFLIE A EPKIKQLD DFLVQVQNQF LQTCHYSGYP DKDVKKIKPD ELFKQVAGFA RQVDAIRKQK QEIADRELKR KEAEAKRAGK GL KSTAVKK VGMK

UniProtKB: Formin 1

Macromolecule #4: PCR11, TGME49_209490

• Macromolecule: Name: PCR11, TGME49_209490 / type: protein_or_peptide / ID: 4 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 56.836012 KDa

Sequence

String:

MAWILKGEFE FLVSSLAERL DIAKCRGLLT SSLDMRLVCS RDPTFAHYYG AGKAYLGRYI EKHLNRTGLG LQSKYMHKSL GASIVDECN LAHIFQILRV EELLECNAQC LPVTVAASVL LAIVGELDAV RMDLLNMKKD VRELDNFRRE SAQFLTDTLE F LVEFIVES GRDRFTYIKE ALILPTATPP RPIPSLGQQP PKSSQKVASD LRSSDDPFSL AARMAQGDAI AEGSDGGQEY LT SLYALYR RGDLQLEILL NSGSILSIWR RLKALFELPR ATAQDLIRWA VIRQAPRLNR LRQLIFTRLL QVLCNPSLRT GKI KGDVPD FPRKALKQDR AIAATVLSAF ARSGVKAWCY ESESQKLDIV RGMGGGEVSS KISSMLCIKV DRGFIDLPMD DDYF DEDTL ERWFSPRWSE TYPNQGRYSS LIVTDDNLYR LVPRKDKSDF GFGTASSLTS LVGGRMRGIV PDDVLSGEHR FFTAP LTTA FLDTEKFDTT LREFFSSDDV C

UniProtKB: Uncharacterized protein

Macromolecule #5: CGP, TGME49_240380

• Macromolecule: Name: CGP, TGME49_240380 / type: protein_or_peptide / ID: 5 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 545.69075 KDa

Sequence

String:

MEGEDKRERD EDQAEKDMAS TTEEQERDDV TGLQREQIPQ GMNTGERGRK SRDEGMLGVG FPSEAEPGRP QDRESTSRER EEHVPHDLF ACDRPFSLSK GVPARATQEA KQHTFLHLCS PLFSESAGTR SVNPDDNEGS QSDTEGGEGA GAQDGRIRVS G VRRAKEAL ESQRSIAGPS FQQDDPTESH ELEREVNEDA FHPSGRTSLS SLPSLGRSHI PLVESQWFSG RPLHFQTQND LR LFPVSPP LSFSSPGLKR KERGKSGLTL PVREPEEGEK QLDIVASFAR LIKDIGRRTS TRLSRTDQGE PASVGEGGRI GLR WGTPGS IGTESSSEEG NSTWREEVGE EPNERRVGES LKNLSELYLD PSLSDMRWEE NRRERSLEAP KLSKEASHGA NPED QERQE TRAQGESRDG ASATLQRIRR RTGIEGTMPY RGNEVTTHTL ESVQEQGDRD ASSFTFQKET PFFTLFCQEL NACGR VRGG TGKGEPVENV MSECRRNTRS SSLPACSSSS SSRIFSDSPL ARFRLETRHP GGITSRSSQL PALLSSFPPR IATQSF RFR RRYSHGNVPE DIISAPSPAS ASSPPCSFAS PFRCRKHSRG SLCSTCPIPS SSSVSRCTDS PRRTGGSESD PCRLSPA AS SLALLSPSSS SSPRIVLHVE ALSESYKEPE GGLSSAASSF HSASPESTCA VSRTRLRRTE RRGESIRRSS GNTGRVSP Q LSRASPACRR IRRRSGDALG EHRTEAEPRQ PPRGRKGERE RTDKSARGST DKEEAKRGEH ATRPLFERPL RLSSLAVDK VSAVGESDRE VENYQKTTSA SEVAGQISGF KEKDIYVLSG HSGEENHAAS SPSSHSHTPV STPEVPATAG GSECFHNGRT RVLSLCRET GANSQKPFTL IPAPKEGKWK EAKERIEEIS TVNVREAVQK YERVGPHVSL SKEELEGVGT ERENMGGCTV L VKKPSVST PDEGLFPFPC SNRPQERNKI EDGERDQGKP EREEHGESSW KQPGSETDEH LVRKECVSKD SPENRLPLFA LY LRQPIAE LPYVGKEGTG DETRTEGCRR GEATQREASH RGQGIDMGKE RRGSYGDGNQ AGTAQPGKEP VAEVNAQEGQ RSR QAERYP PTGGDGEDNQ SCSKLLPPGG APCKVQRENT VLCGLTPLSS LLVQKSAVEM CDDAEGREVL APEKTHTRDR QRET PNSVP INRVDGGAEL AQANEVGGKR EGERRRAATQ PEDSKKAENE GEEAGMQDHS GKEDETDTNT SYESLQEKPD GVEDN CPSN CRKESDPSCL SDSQVQCLAE TFWGFCRASA SSSLPSSVSS SSPSEHSGSV SPLFASRVTS LSSSVSSVSS PAPFSP SVA THSSSPSPSA SPRSLCSSLL FDGDVESGTE EQRTSPASPS SLGSPRVSTG RSTGDLSGSL SRRFCFLRSS LLPRRAR AN GPGHKKKRIT VRKAAATPVR PQSRQDQITV SNTHADSGAT KLGSHRSRKE EDVPGPGENP FEVGMDCGRS VRDQGRFS A CALSQSSSSP RVTPFSLSLS PSPTDTSSLP LPRCERTGLS SCSHRSSRDG VAVSLDPPLF VRVAATATSM DMSAYPESS MSETKAGNGG EMKDAKDAPH IFREQSDGDG RNANQEAQVS RECLGSRGVG ETERQMWTAG LGRPDRAEER AEDVEKPGEH GQSEERRNR GRRRPERSCA LKKESQYQEA DAHTTSTPSG TPASRHVAVG ECHHAFSASS SSASSPEPAS ALSVLSSCEL P AVAGVLWS PASPRGVPLA LDSDEEPAGE GAGTAVCACY RFSEEEEENE VEHYHENEGR KEQCGQEVLR KGQNPGESEG GE ETTKGGA AREGTKRTVE QAEQEDREDD LSPGKYGREQ DETSSYKGKM VAASEAERNE DDREASHTED ERGRHDPNIL RTR RTRECE EDALRPVTPS SFPPQFAGRP ASSRAASRQS VSAPCRRDPD PQVVVELSDE EGDTFGDAAS SDASRFFLVV PRAE VEETS ESCLTTNIGL VYSCSEEREE TGHAFPLSVP PVLVTDDERV SEREVLVESA WVATERQRHE GACLLPNTKG GREAA GGEG PQLEDAAVLL TDDDSEAEEA NSSGELWRPP RLLLEDKVEE IEQEAIKSPS DSGSELVCRF VVAEKRSEGD VGDRAD SWP AREVDEQIHS MRTGQRQGRG ASTATEISCL ARPCECVARK SGERSEDDTA TVSPAHNRFV MHPAVAAPPH DYSSSSP SS FDKDQLCPRR MRGEWTKAHG GGETEATGDI EERVARNKGS KTDGGNPDRE PRREKTGLGH RGRVAVDNAA AAVPPHSV K RPGGRTAGKT RQEDRHGKGK RKQARGADLR QTEGAALDHE RRPKEHRGQA RGHLSRRERQ SDTPGKLCWR VDDSRRSDG REGRLTWGTE AEISDGGPDA QMKRDPNGEQ LKDGTREALY ACSISDEEAQ SHDFDEDANG EALAFQAASA GAPTTTDIVA GCFHSDPSR VGTFHSRSFA PSSPASPFSS AQVSCSVASV ARRRHSLALP LSSGGPGSVT QASEEAEVFW RRMSTGEVGA R EKRSHSSS RGKREEAPFP SRIGRRPPLP SPSGASFRVS LSRIGEDEEG ETQGREEAQM LMHSSVLPLK EEAGFPTAGK SR VASHSMH RGASVPKDDG STTRPVEATS EGDEDWVLSA GVKGERKAPG KPRQHAGNDE HERGSGDERH RRQKPGEARR SPV SRAARV QKQSTEASAK LYTGGNVSEE DTRKEDTRPS DGSEERRRPT QSESREVLPL PSAPRIQRRG ETDVARTRIV DKKQ QEVLH AKGDLPTGKA AHVQRFRSVL IIERQTDDED EQKSGEHRAE RDPDRSSLRR LLQGSRLRIE TDEESSEDER LAPSG GSVR MPSSQSRFSS GSSASSQSSF SSPSFSDTSS GSRRETVSRP PQKRRNKTKK KRHCDIPRSV PADAYSDGSV AEDMRM LRF ERRTSEKKTP GRSADREATV EKTERGKSAE ARARDEKLWR RGTRVFMEVV TDTEDEWGEE TTEMTTTGWK KFSEDSR VL SSLPGNDISE EVDKTRQISA ESGATAKGGA RSPREKLEEP RVAREAAYPE QLTAKVPENQ ADKVTARSDD RVARLPRE S SHIAHSHEKL TGTLHDQKWR SPRSEKGSKR TGDASCAHTP VGGTHRGSGG NRQDSKRNQP LHTQKDGEEG EGDGGEANE DRGCSGDILA RSKPNTMWRA THWWRAALES FFSMSLLSSY ELVGTKGAAG VLRGELPGPN SVSLLSSNWH DRLQWSFRQV VEEARRLGI VAREKWADKE LLLCKEIGYF TAAARAAAVV LSHTHPAGDF DAASNQEFLT AEGDGVWLRR GRGADDTDRK G ASLIRKGK MSQAKPGVAG CFVEVKPDNL PAVWRVYRPK FEDDKVFVFD SLVLTVVHGS RRHGVSTRSA RKVYANDYKG RQ AAASVIY QMNLTADLAC DAEAEEFLMS DVHRRHLQFA RKTQDLDFGR TDYALPVCTL VDYAGQRIMV EPLMPIEPEP VNV EKELVE ILKKEMPFYV QQCKARGSEY LALDAVDKVD RKVDRKMRFI SKSLNIFGCP FPFGHSDFDS KCRIPRAHLW RSRV DQLCV LRNLQELLPP AVIKGKVHPT HRMRERFVQQ IFDIPLPSTA AAPWTAPKAE NTISKFLQTT RPGSGLELLE VAHDA LVEC VQDDLVPTIE ALHVNFLDSS DVSHALHEQG VNIKSLGLMY NRFAGAVFRD ASLREMLARA AKHLFLKQME VLVRKK AYR KVLWVELLQT TIVALFNIVL GTTEETRSFW QEQLLPEVAE IFGVDLVGTV TPRSVCHPSL FKAMEFHLGV KFEATVF QK TQTGENESLF LQLPFTPADL SHFTSIRDVA LFPHIDFIRN LVTNNTSAFP ASAARSVEES RFPTSSTVDD SRSRDASA Y PFGQSPGSES VQRHRTKFGE CTEVDSHSSS LFAPRGERLW EQRDGDVGEK SQEADGGLVL NVSDAGKKTD TTQANNVAS LRTFCGFFPQ VKTVVPASLG IIRTAQEQAE RFPVTRDISL DVKIDQMKAG LTLTRRLPMV YGTAGMASHG LNGAIRASVL GVAYPCHPH CVMSLKRSPT LTTEAARGRG KGVLSQRATS KRDTTELEKR LRKSCPKALG QTERCLIFEG FRISLALFLT L HTMVASDI VKIGKIVTQL AYVLLQHGDT RQAIAAAEYI HTRLPDIGFL HGDAKLLQLQ CRAKTSKLHS MVQMYKELVP DF LFFEGPL SLRLMLMELL IAAVAFQHKQ YAAAIPPGLK VYHASALTVN PNGCHWTGVA ALRLIGKSLM ELRRFSEAIM VLE DAVRGG RKDPSLPFFV TCMNRYWLAV ALSKVGRFRD ARKEAAGALV VLEDRLGENH PATLSALFFV AELDQTVGCE DIVA PPLKI MDGEDTLLRE FGKAASVDVG ELMLYRPIGP EDLEIVEMVA RDRRRGKSRQ SAIKNFTTLF RRVLSGEKYL LVTQA QYQD SRDQGTRRLL AAVKHTLTVK MCDLSLPIQR AVAYRLYVLC VSQSGSSFLR KLAAMDRDGV MGKDEIERER KKVTKH GDD SSFGPGSHAT GSKAGKDAHK DKDLSAHLDD CFIFHRASSK YGTRIAPDSE ILSTAVKADP SSLPSREHGN LMMTLLV FS PPTSDFASML EDCIEDCRQE PGLVPAVWFD RLVGRIVAEN GTPNDLVCFI SMLRAFFSPI QKRIFLFFLH EEYARTDL D PSLFHSHAAR RELYIDTVRK LIQVRMNNAR FREYQRLKRL QEKNKKDRKK RKQQSHGSLT SFDVTESDWA HLSYLEYNI EKEDPSDAEE YLDTTGLING CVWNGISLQN LNVRLSEQLI YQDALEKEYT SKANVPGMSI VKGKDTESIT VLESVVDHTD RRLLGKGWI SGRTSTRAMG RAAELSLQKF GGQAGLVTQS VSSACLAYNL DKIERLPVPR EEDDLAWRLA VEGGFLA

UniProtKB: Clu domain-containing protein

Macromolecule #6: FLM1, TGME49_271780

• Macromolecule: Name: FLM1, TGME49_271780 / type: protein_or_peptide / ID: 6 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 303.103719 KDa

Sequence

String:

MEAGRPLSLG AAPPGLLGPR QRPSSFLLPR TSPVHLVHPS PSSSSVSSLY RGHGKRGVAD EGSCDGDPED TRVSVDSSGT GTDDEGETD EEFDSNRTRI STFQRITNMR HRSCPPALHS PLSDVIVEDE LSSDGATRKP RHVYFAPSSS DESSPKSSVS A ASQEPADD SSDAHPVSHA RGRHACRAGS AFPWRSSSSS DCSSAASFSP SGSSMSDAST LAGATQKPFD LHAQSPGGDS WR LAESAVS RPSKALLRPP PAATRSPVGI ARPGCMKKPS LNPSCLSSFS GGHVASSLGP PPGLRSAASS TSLACMQLPE LRC ETRRLR VRFAETTVHA SGAEETDKGT PHMSPKESLM SPPTSPLQSP SQPPSQSPHQ SPSQSPPNSP SQSPRQSPSP SPPS SPSPS PRQSPSPSPP MSPSQSPRQS PSPSPPSSPS PSPRQSPSPS PPMSPSQSPR QSPSQSPRQS PSQSPRQSPS ESPRQ SPSQ SPRQSPSQSP PKSPSQSPRQ SPLESPRQPR PGARKQESKE EIKERPKAFG RPKSLTKSED PAVLASDDKT ASVVEA PVE TQGVMRSTSR LLMDQEGSSM RPKRSESSSV EARSRSGRQG LAICGPRLAS GKSVQSLRSM NSLASIAAKT SGATSPE VG QSQREITGAS METEPPLAAE WPSAKNDKPG STIASSSRVP PDPSSAKPTR GDIKLSPEAI LEAEESINDR SENDKTNT D GDASPQSPKT PVDLTTGHAE ADGTRICPDG ADESAEASEM PPETQRPLTT EGVTKVREER ERAADKRKRH EKHMLSTQA TLRLHIASIT NLQTSGVVPK RGGYPLPQRA RGGVSGANGA YGLAPCFTIC TVAWFPEEDP LEILGFAQGA SWLPGWGLRV SSAVNTRPS ARPGAICLDD AEVQQELNIP ISILESPWQQ RMLALELGSN FSLGVPEATP ASSTGMSGKQ AVKAVMKRVS Q AFTLWPFL LVSVIQIHPK LSHSLTIGNS PFRPAPVGTQ PQNAIGPPQR VRYPTKSPVT VVGTAILPLK QIHEGSGVLV DR HQLLFMD IDKAPPVAVH SDGSLTYLQA CRLFRHDVIR EMISTYREGL KRLEPVSLLS VIEAYSGRLS PVGEIFVTLN QLK PLTINW GPVLGRRWSH PDARRAGDVG DRKGSRGKTA HTRRLKDAGD NLQEEKNAPV GSYPVAHAVW IEIRKVVNVP IYGL TFRQI CEAELSVVAM WEAERACAAI LRRKSRKITA VTRCEVQPLN ISAIERMSGS EDSLLSCYIR GQVCLPVYPG LDPSV VLYL CLNGHIYATL ASPLNLAAFS PESGLGGHQE LRKKEGTAQL RNGKAERGPL SGPGQTGRWR APVSSRTPLQ NEDANG SLA ATDEDDESEK SEPDNVEHTT DESSFRAYKL RSFVPEGPQG DARRFAYRLY CGQIELRVFP CPANGLFMTA AEFAKLN IE GRSNSGPSMQ LWPDSPNEYG APVIGPFGKW TAFADDFEGR PGLENLEGGV EEDEEAPGSP MGSRPVCAFF SPAPSHLQ P LPGTPDEMRS DSDVASASSE AEPTPTSLSR PSPVESMSPG RATHRGGARG GLGTVPRGAG PVDAQMCVAE GANLTVGHV GEWNMFEVRV RNRRGEEVAD ADGAVALCFS PLGEPQYSFV ADPTSSLSVD AYRMDTMLIG GGRVGVITKT ELTSLTPEWA VEKTEGDCV YRVKYRHTRP GFVLMHVTYE GLEIAGSPFE VHFIRGKAVA EACRVIGQGA KTCFASPSPH LQLPYVNAEE A RALIDQRA REEAWKTGVE GKGEPPKPAV LNNYVNQFVV AVCDEMGNRV SIGGHRVSVR GSRGAKILQV VDEGCGTYRV DY AVYLPKT PLQQIDVGLL EKIDSYAWIT QAQPDVFVPG IAEAYEHIES LELPIHTEAE IEVLLNEQPI FGCPFKVRIC NFV EVFNAY AELDRRSLLG QQVCAFERVL EEGNYEEATS MLWKVQDMAD EKQLKEEVVK VLENLVRKQL EREDAQKREA EEGG CTYLL EETSKDREEK RRREVEEIRS QWEDIHLLRE LLLRLNKNCT DRESLVHKFT LSMVGRLQLT EMCNLRILAE HHNMQ RLQQ ENLMPLAEVL QAQYISLFRQ MTQEIVALVK DVEDNKFQNL EALLQAYRRI GVQLRRLHRY QLADQMDQII ECVVEE IEL QRLASIARAK QEKIQEIENV IAAKEQALEA EKKKYDEHAK TFQPLDMDEV RALITTRRQA GVQTEDALSL RQGIFGP LI VARATGQHVP KASTVVTTVK KEWRSCNVYD IHTTIKRLLK NCPRLKHCLK EVFLYYAKVS EANVTDLQSD GLPVGLPQ L SFLRFCNDAR VDEHLLADRT ELQDLFEKFS IEPDTGRIRA DGFLRVIPVF LWLPCLRELA YLDLLHVICR EALDKGEAP RVALRRAHPS RLTAFHFFCR ARFLPLYDML YNNDDFRIST SVIMESAAGD VLSTTAESRE SNTKHSIWTY AHERLPGDVF AMLKLEPVE HLFVFYSELS AQPALGRARA EATLPTASKG SVAPAKKGDG AETKRPPKAS PKGQEPQRPK PKAQVTGRRF V AQTSLEET GAEHVSTRAW VSLGVLIQMW REFSVIPGFI DCDAAMSIAR VAIGGSRGKK NAVRKTADSQ DPGGGDTGVP NA KDSAAEE PEFRRLLSMP EIQGDTRRGP NGKLRKAAPS PEAEVGRLYF PNFVEAVVNT VCECTARALV QEGDSSVPPG SRE ATSAEI KKKAFFLLHL FGINDVSRIL LLTRRPLREA VRTPAKDETQ NSGVPNAPPG T

UniProtKB: Filamin/ABP280 repeat-containing protein

Macromolecule #7: Transport protein Sec24

• Macromolecule: Name: Transport protein Sec24 / type: protein_or_peptide / ID: 7 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 108.131289 KDa

Sequence

String:

MYTQPNLPGG TPSYPAGASQ ASRDAAPTQQ PFYGYTSQPS PSDMRQGYAN PPAQSYSNGM PAYPSDSASV PSYPAPPLAS GNPQPAAGV YASQRSAGGI PSSPFHAATP ANPGAAPPPR TAYYPSGVQS SGYAGYGAAA SGVSHSAALP ADSGAYASTG S AAQQVLQE IQQFNSPRYF TRASVARVPH SSSLQQKLHL PIGLFIRPLA PIRPGSPTVP SVNFGNCGVV VRCKRCRTYI NP FVHWEAH GRRWSCNLCG YVNDTPQFYM RGLDEKGKRE DRFERAELSM GSVEFVAPAD YMIRPPQPPA YLFLIDVSAT AVA SGLVES ACAGIRAALT SGKLPGCTDS GAGRGDSRTM VGIVTYDSAV HFYALGSASG GGKRRPQVLV MPEIDDVFLP LSDD LFVNF AENRDAVLEA LDTIPSLWRS NGCIDNCMGS AVKAALLVMK HVGGKILLFA SFPPTVGALV TKRSGGLAGA DGAGT DGGS RQQPDREVEL LKPASEAYQQ FAQQLTQSQV SVDLFIAPSS CASPAAGNVP PTGTYAGGKP GSGAPGLVTA PYCDLA TIL PLAELTGGEV RYYSAFSRLQ HSQQLENDIV HIITRTTGWE AVMRIRVSRG WKISARHGRF FLRGTDLFVL PTVNADS TF SILMELDEQA GAGGPGNALV TPPSGGDNVV AVQAALLYTN SDGERRIRVH TFCMPVSQNI QDIVSSMDPE VVTCLLIQ Q AIDQSLRSKI ADGRAFLQTS CSQLLSVLHS VGSSTQLAAS YALPSLPHYG AVAAASAGNP AGGALLEAGR LVALFILGV LKSNAFRPVK EVSTDKRVFS WSRLLALPVD RICAYVHPRM LPLHNLSNFG GPSDRVLDGQ EEVILPPALR LTAEEMTQDG AYLLENGEE MLLWIGRSIS PQWLASVFGA HSLDCVHPDV AAASIGDSQD PVGLRVRAVV QSVQGEHLCN AMRLLVIRQG D PSEQKFFA QLMEDRVPSL LLTFAEFLQK VDMRSQLQAP APPMIPAAGG AAPPLQQPRM Y

UniProtKB: Putative transport protein Sec24

Macromolecule #8: Protein transport protein SEC23

• Macromolecule: Name: Protein transport protein SEC23 / type: protein_or_peptide / ID: 8 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 87.697195 KDa

Sequence

String:

MDVHEHEAAT GCRFSWNVWP ATRAEAQKIE LPLGCMFTPL RDCTSLQLVE YEPVRCRVSG CILNPFCAID FRSKQWTCPF SLQRNAFPN HYAMHISEQN LPAELLYPTI EYILPQPMPA ANAPGVSAGE SLPPPLFFLV VDTCVIEEEL DQLRDSLMQA L ALMPSDAM VGIITYGAMA MLHELGGGSE NVDVMKAHVF RGNKELTSTQ IQQQLGLARS ANAYVPAGGP GAPAGSPLLR PA AARFIQP VGECEYKLTS ILEELRRDSW PVPSDSRPMR CTGLALSVAL AVLEAAWYQL PARVLLFVGG ACTSGPGQVV DLS LCESIR HHLDLQKDNN NARFVKKALK YYTQLANQAV RSGHAVDIFA CSLDQVGLYE MKVMTEKTGG CVVMGDSFSI NVFK DSLRK FFETDSTGFL KMGFNAKIEV LCSKEFKVCG AIGPCTGTGK KGCQVSDTIV GEGMTTEWQA AALDKESTVA FYFEV TNQQ AQNLPPGKQS FLQFQTSYLH PSGRRRLRVT TLSYRFAEPN TIDVAPGFDQ EAAAVLMTRL AVFKTESEES LDVLRW LDR KLIRLVARFA DYQKDDPSSF HLSTEFSIYP QFMYHLRRSH FLQTFNASPD ETAYYRSVLL RASVMNALVM IQPALLE YS FEQQGPPQPV LLDAQALKPN VILLLDSFFH VVVWHGELIH QWREQGFHNM PEYENLRQLL QAPLEDAKVI LDERFPVP K YVQCNSGGSQ ARFLLAKVNP STNYAQTAGG GAAMAGLMGD SGAAGESFIN TDDVSLKVFM DHLIKLAVQS

UniProtKB: Protein transport protein SEC23

Macromolecule #9: FLM2, TGME49_285990

• Macromolecule: Name: FLM2, TGME49_285990 / type: protein_or_peptide / ID: 9 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 131.144031 KDa

Sequence

String:

MMSLHGKFPR PHRRFAFEGV HQMDTPAEFS DPISQSETDH NGQMPFFESS TVDSGRPLSG TSDQFRAANT PSSGGHSARR LGSHDSAKT PSQDSLLALL RSLGSDEKKA DFGAPHSAKN APIPDASVPK QTSSKTVFPA ASVQNPTSKV ASLSSRLAVS T GRVPSKIS SLSSLPKASK RTENPGSKAW SPPSLPGAAV ERLGSNVTPM TGRKASLQCR KDSSESLWPA ARLPPTRPGG ES GGPIEKV KAKEKGKLSS DSQTRLPLGD SMDDDEGARR GSELTSPNAR PTLLHGRSNL HPLESSFTVF DATSVPEDER CAD TVSSTA APGALSAGCW TAHKQEERSR EERIFPLLTL PIQTVSLSDI QDTYKAFSKR TDPSTQGSTQ GRALPWVLAA AAEA QRDRE AELENLSRAK FPLVSDVFSP EDKAAYLGML NAYGNPGGRS RVLLTAPSST LFVPPLHEDL ECGEFRPAQD LNTEA AASL CRVFGPGLEH AEAGRLATFT IQAVNSDGTF MRSGDASFTV YVDPVKGSAA EQTGIRWKNE PDVCAEEDYD SDAERL NQY RPPASRATHR RRKDTLDGYV YNHGDGTYTV HYYCTRAARH SIYVYLNDQI LVGDSPYRVN VTPGSTDGEN CEAYGDG TT FFALGGDFSE IVIQAKDAHG NKVKTGGDKF TVAASGAAKI VEITDMKDGT YWVKYYIPPG AERHFVEISI SYHGRPIK G SPFRPRPGGP LEDEPPPARL CDTVNVANPS DSLMTLAEKC HQKWSQTKNP PALSPYPPFP ASEEAEAILQ RANLDYDAV VMDTTGEHLR ETTKTLVQYS QDILNRDSTI QSVLKSLKAH GHVGNVYDAV EKGNRERLKQ YLDVIAQLQA EMDVTYKSIH YGVVDSLPV ALEVEDPCEI REIHSKRRQA LIQIHKNLEK KETELRQREA RFKAQRLKFV SGMAEELERR REAVDMEKAA L KENTQYIL HLTQLSLKKH LRREMLIACE REPVEAFKSK FWQRDFNRML VDTPALSEGK AKAGGGEGDD KDKETVEEYH PA PSIPDDP SRPSMWLHKG EFNSAGSTDF GIPKIKHDPS ARLIRERRMY GGGNPTFVSY SLGSSPPPPN SSEVPKKEEA PGK RQAVQG HRASPSGSES DAEERPKQKD LKEETGRERV EMEDPRYPGF PFEDLPDELA IPYPRTPPPP PPPRYL

UniProtKB: Filamin/ABP280 repeat-containing protein

Macromolecule #10: PCR12, TGME49_292170

• Macromolecule: Name: PCR12, TGME49_292170 / type: protein_or_peptide / ID: 10 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 175.593656 KDa

Sequence

String:

MFEKMLVGAF KASHVDDATF SLASADAATR DELLRRRRAP SHRGKQVLPG GPLDGTGEER RENNTVSSDG RGGVQLDAGK GRPRSVAPG EDVAAARAAD PVKDLTAERR HGVLRAVGED APLAAGSARR RKGSALRSEL VRRMEAVDES NRQQDERELD L VRSTIPKL LSRQQFSDAE SLLEYYLDAF PSRAELYILA ADLNLEQKKF SQAIEFYWTA LFCVPENRML IRSLRDAEER LL AEVSCIP HLYKAALFDS SRFASCHTTP RPYAEGDLYF SHPGASVWTT CESLPQLSSP KALATEIGRR GALQRPPFLV VKQ DKQTVC IAEVPLEPGQ LIFRELPYVC TPLTLEGGQV FTTCFHCLRE RPVSNRGFSC PVSPHTCPFV FCSWECLMRN VRVH SRECG LLPMIMAAAR EAELSITGVL HFCRLLLRAG LEREIVRPEK DMLYNVLGEL QSFEESVEIG QPELRRKLTT LARRL EKDF PPGFYCYLQH RELIRLMLLV SQYSPFVRST SPSAAVQRRD PDASVGLVFS RRLANLPHSC VPTCTYNLDE DGFVAV RAL CHIPVGGRLC VSMVEDLFTP SAARKDIEAP PRVFGCGCVR CSDPSEGGRM LRGIRCGVCL RGYCAPFYSP ALQKRLV AY GVGGAEVARK IALLACAVEK PKAGRRRRST SPLASRFEDP RRASETAADE AEERWQCTSC GNASELLSVQ CDAIEREV T QLTKKADRRF VKGKSLPARK IYVDIIRRFN SRLHPNNFLL YNAHVIAAGL LFRDPGQDVP QALLYIRRAV IAAEAVLPI CHREKIHLFT QLAQISFAAS MADKLSRRGV GLPGGFVLEP MYSAYWNAAV CCGPRSVLAL TTLQQLRMYA AALNVATPPA VVSYKLPTV GVIFSYFRHV LGRHTESDRT LRRLVEKDPF RLATTIARLG LHCLMSLEIL SAFNNVHHLG TGLSVMAIAA S QSRLTLVR KLLDAGFDFL RGNELGVTPL VAMAAMPLPQ GGRSVGGGAN GSVVDGRSSE QKAADRLDGA GVGLSSDAEP GE LYFRSEV ADRADRDQAE ILRLFMQECS RRDARAKEAK TKDQLMLACT HRILGLNGPL HFAAARGKTE LCRQLIRSGW NVS KSNGEG AVPLHLACAS GDVQTVMVFL IPRATRHQDA DAGDGAATPE KSDSLGAFGV DINVKTERGE TPLMLAAYAV QRDM VKLLL DKGADANVVG DREKMTVLHA LAAGVCRTVE VQYEPLDPRQ DLAAHVVMGL NHASLAANIY NRGPGYLYVT DTVED RIPV VSQLAPDLFI FPQEILRRLT EANHIGEILL RHCDHQLFVR KTKKGYTPAE LLSHLWERFF QTRNEVVEQS ELRLAA LSH QERQEIETGW KVAGQLAYRL IELLRAGGAT VEDARRPEDM TPRRTDLQER RLRQLHRDAP WLKAEEMLKG KTQGLSP AT PQSRGHEQAA EAAKSADPRK APLTGQTAPP APPEAKAEPA GAQEKKAPPP PPSGEKKAPP PPPSGEKKAP LPPPSGEK K APPPPPSGEK KAPPPPPSGE KKAPPPPPSG EKKAPPPPPS GEKKAPPPPP SGEKKAPPLG KKKGPPLLGK KGLPPPKK

UniProtKB: Histone lysine methyltransferase, SET, putative

Macromolecule #11: PCR10, TGME49_298010

• Macromolecule: Name: PCR10, TGME49_298010 / type: protein_or_peptide / ID: 11 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 261.711625 KDa

Sequence

String:

MLQLAEASFQ NLSGEDLLSG KSDRNEKASS FGTGQAAVAV NVSYFFLQVE IAQELPEGTP LALAVMYRDE KLCRVCQQPE RVVFKSVYS RRPKEPLIMD INRTFFLPFF YIFPSGDPAL DIVIVHESTN KLRKIIARTR LDLGPSYRRL GLLRRAREFL L LPRMEPAG FIKLAARVAH FPDGSLPSSF LWDLDVLKRK ADALVDGIER GRLAHKDATL KSLDTEERIH EYEGFLTKDH GF AGCTAEA RATESRSPGG DTGVSVGSER RQRLVHEAQQ LQRFEETELE SWLETERLSK LQGAVAAGRR APESTDDSSE DEE DRMWRE LQLRRAKRRQ KEVEAVMFGG ESEAARAMRN IRTRALDLAL AKGSMTASTL ALRALNEEFD EGNDEESCAV TVFP RLHVK GAASADCSIK NLASLFLETS PEEMFAVCPV PLFSPERIFQ RINAFMKRGS SVSEGTSIWL RSPQHVCNKL LDKVV SRDI SSARVTDEID PKKNTVYWHI LASCQCISAS SAFAFVATNT AIEIIERHIY GISEDSLLIG SLPFDNPTPS GLFLRT KVT KANADIETRE SIAKSLQSLQ KRILLAQQRA FNAVSVAELE QKKQRGEVLS QAEREQLSKW KAEGVGDKLQ LQKKSPR TA RRLGRQQSKS FADEMAAEWS QGKRERVEGG SVSGRGSANE PFVSHESGRR QGWKRQVAPS GEAREYVESS RLDDGLAK G DKLDAHHAAR KRWTTFEKDF VFDRVERKAR ERRKSLEVLK RQWAGGDLDL CLLPDNHPFN VFRLFKFDRM FMARSRTEA SSCGCQLAIL VLGDEEHDIE EASKLYGIEI QAQQVMNDAI VELKRRSGLQ AETREHSTKD GTKKRHSERG PFTVLQVHLM TAIDYKGFR VVVFSLPATV PRLLNWPQPV SSQLSQELAF VEQRLNLASL VKPQTWKGSV KNDEILMKTV LLSLDPSDDD S VVFYRLCF AFPQMDNSST EEEEQRQPSG YVSEGRRRAK LCERIRCNAF VHARDFELAI RHQQVSQQSH EGRVCTELLQ VV KTAETGN ASHLAGLQRL SRGAVISTYL DPLVHSPIVN MYAPDEVLMN FLLGHTPSGY KETGEPSIAG PFPNSRARRL AGA VRTEIL NKALAELEGM PPFTPMDSIT LTHFFHLRGI NMRLLGHMIR GTRAAWLQHM LHVEVVARTV KRILRDVLRS LVFS AHGYL QTARARMQRG MKKLLKRPTF DISAARDFRM FHPGDCTPEE SSESDDDDDS SSVSSRTTAD DSVDGCHAGE RMRPG YSRE SLLRETCLNA PFDIATQLLD DIPYLLDLMS QTFRHFSWTD IVLLNLFNLT LGDSSESDEF WSSFLATRCS QYFDVE AGS IAKSRISRPA LYVAMQHHCG ISFLPDAGAL NTMRPSAHPL QPSHLRLWLP ALRRTIELPP RLPLLRFVTN PGMIPLL CQ QDADLIVSPS PSQILLSKFI GHTLVQCAAP LWRVWDAVEK GLDSDNLLAA YVSTKAHPLI IRQSNWHLPD AVLDIVGG A IRFGVWKVAR YLAERCLNFY PESHAAATQN RLLVFLIELH GSGLPTSNPR LVNLIGACGR AIKDHWEDTH PVRLDLLTY QAWHMHVSGD LSECAWVLRS ALTVAAAMIG NRNYTDRNEV RKLLMLTSLS LKPFRQQLEA TTPRQVTNLR MAGLLRLLAR AKLLWLLDL DQKRKCPTFS SPGNRSDVAT EACNCLQWAL DAYDFHLGSE TPLTVGTAYE LAMAIFLLRK ATRSDEALLL V ETAARLAE ASLRVRTQLF GRQHVTTLNT ALLLGLLLAD QGKHRRAMSL IEAVIRNSFI RSLRSPARRR YRPEDSFSQT LW CLPDAFF YDTFHTHGRI IQPDALHDAL LAHHKRRIEA AAMTRRDGDS KEECSRQENK QVPHSGQDTQ AENSGDSGPP FKE VGQAAE KHKNKPAAGD HVEEASVQNK PTDDDSEDDD SVALVTFAEQ TRDSYAFLTH QDEVPSDHLI PSIQDLEETL IRIY INHGI DSALERRLCS LFLTLDVLER KDYQPMSVTL ARYEQASAGD DDQCYGYLRW LLDSSNSSEM QALEYLQHYP NKKSP AEIF DPDGDLSFSI CHGTPPTKGS NYCTALDRFI SSLVSKAQVA IIDVRRRLLR GEKPKLFART EFPSWKRKLL KAMQKI WCI LSLLDVYRKR APPDKDAEME KTKHCPCRML PDDLDPKMLR AVVELLGTQK FQELFPNQPI RDATIHKLSE EQTVRHA SA KARTGIRLPL DLRSEALDML ASLLYHFMDV RIFRQRWRRR FKDSRGAVHA EDRNDSNNSV VHLTEFEQLD GVSS

UniProtKB: CLU central domain-containing protein

Macromolecule #12: PCR14, TGME49_311880

• Macromolecule: Name: PCR14, TGME49_311880 / type: protein_or_peptide / ID: 12 / Number of copies: 6 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 81.526453 KDa

Sequence

String:

MDKLLQPIAI SSRLDSSDES SDSWVRSRRR IYSSRHEGNE SSPQRGNRTL GRSVGSDEEP LDGPSSQANE RFPRLRDLKK LFSQVPSRT QKLWKYRDEN GHVKGPMTAL KLLKCIETGF IKGRGDKVLF TSVGDSEPPR RLADILPEIL KDANLSMEKQ T APPVADAG GNQEIEVATE SPMQFADDAF STEAVLDIQK LHKSFGSCDL VVWLVPPQER LQHLAEGATE SQFTPPPKVF KI PVHSALL RISSPIWLKD LTVVEAKHRT KMKCNRDDDG RVLMKYVIES FHPAAIRAII QYIYGYRHEV LQGDPFLQVA VYK EASRLE MKQLQKEVLR ALSAPMNIGP LVSLAKAAEV MKVDQLLLEA IRILAGCAYA LFSGMRHLEL GPKGLQLLLL QDNI QLDEP QIYICANAWL AQQGVSHASA SYRAASFADD PLSPSLAPHR RKERDFRQAE ELAGTGSNSL TEPPPGVQRD MAMAV YRSI RFDSMSPSQI QVCRDPLLDS LLLEACLRKF SHLETKPRVF PWQGNDQFTV ERDGGLYPVL LKRTSRSAPR FREAGT EPL IWAWTLGDSR LINEALWGIE VVRTAKGRLH FGIAVEDKTT QSRQVCYLDP AEKAFVTGTI TNEPTAVSYR TVQPGGD VR WYRRLCDGDI VIVRVSVTTS AIHLSIQVLK SRSMRASYML ALRSPVCFPG QLLQRPFLAI WPFIEAVNIG DTLGVAEL Q VEAIDIP

UniProtKB: GYF domain-containing protein

Macromolecule #13: ICAP16, TGME49_202120

• Macromolecule: Name: ICAP16, TGME49_202120 / type: protein_or_peptide / ID: 13 / Number of copies: 16 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 147.400141 KDa

Sequence

String:

MEGTDAADPS ERSRSASPAV SAVPAHFSEH SSCRSLPSCG SDLTQRPCQR EALNASSRLA SREKASSFPE SVANCSSSVC SQSAGNTTP NDAWHALAAE PGSLGCTYTR RSRLSSAEEL AELVPTEPPS SPSDFYESSS CHRCSSPSSP VSAVDGVVHL A SACAGASP ARASPTRCSG ESYADDLSFL DSAQVSGGPA RPPRLSTHLL KKGELLTDDE KAAADNAVSR ELAGNVARGT QG AHLGSFG SPHSCAECSP TPSGAGARDD FSDSPALSQL ERNDQSPPLF QEEYLHTTPA RLDEDEEDVL RRAERVILQQ RSR LEQLSS LLLRTENEKV AAYKASQEEV ERYLEENRKL RLHLDAANGE VAAMSTVHIR QEKMLDQLTS TLAAAEKGIE DRDR LLSLA QRQDKSEENE RLKSEVQQLK CRVEKLTRGA TVGSLADLTD GDPRGAGDVA AQLARVQRDI DQHRGTGKQL GLTFQ ELRV SRMSLMQSQS DLLEAQKMVE EQRLEIQRLY KVIESLNREK SLVPPVVLAP ANALAAQGTQ EQLGVLLSQV HALTEK IDS GIASKPQESE TFSSVHMSIL EEQRNLLAKV LTSLEELAMS RFAEDRSRRR DMDRDVFFTA TLLHGQTDDP EEIPLYE EK LVAVRASALA IFEDPEDEEP AIVLSSHKVR QVRQDYNELV FEIDYEVRPG VSEYHYLRCQ TDDDMEKWLY ALMYSGFV S APKAAPASLP QIPKPKDSLS WIVPSGKSPE GHSVPICQNN ATAFYNQENN MLICEAKGDP PSVLCIPCDR VFLTEDRDN GVIQFHLKEN PKETYMLKAP KETFEEIAEI VHRGTYASRV KPASPKPRQE AAPAKPVAVK KAEPIKADTP AEPKKEEKED ELFIVRDGY LMLMEHANDP EPLLKLFHSD CITTPDDANK EFVIRHMPGT PDEESYVFGF LTDEIYQGWY KKLKENGFLD R KDEGSVRT NQVCVVTQNT LALYRYYGSP GAKPVMEMLA ERCQATSSRE RREIRIIHTT PTGKRERIIL DCATLAEFDR WN VALQFGG FLKSEGDGKN GGRKNYANLN KYVFPVNLFE DESGQRSLAL VVKDRTIFLY PAPQAESPIM CIPAADAQVQ YVV EHRKIR VYVRRNTDEE ERIDFILPLA KDYDRYSTEL QQQRFPPAKE KNRGGSRKPF IICKKNLLAF YKNKYRKSPE FIIE KDLYK VDISSTRVIT FKPLQPTSEK GESLIQRSLT VTTDLQWNKW QFCLKLIGFR SFAESTPPRF FPQIIYGFVS APAAD LRGP GRMFGRLGLD TPKDRVSFAV PEDDAQDLSS RSSTSGSG

UniProtKB: PH domain-containing protein

Macromolecule #14: PCR12, TGME49_219070

• Macromolecule: Name: PCR12, TGME49_219070 / type: protein_or_peptide / ID: 14 / Number of copies: 12 / Enantiomer: LEVO / EC number: cGMP-dependent protein kinase
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 310.184094 KDa

Sequence

String:

METRAMLGAP WSAAEGGDGV ADPDRLYTRS LREDLEEARD KLLKLKKMNE LLEGENQDLR RRLLSQLVNC PDAAETRELA RRYGPELAH AFSSLSDENK YLKVKLQSSQ RAEVRAEIRA EQLANALRSD EEKRYAFGLA RDAARGSISS KVAMLEAGCL G SSARGDVG DSPVFRGEDV PGPVAVLRDN KHLRLRADAN EHCLCTEKLQ ELMDSLDDNL KSYERNVWQL QVNVLEDERT EL QMKVANL SERLTEIQSK IMNDPFLSAK YAAAGTPGAV PNAQTQLREA SARLALAESV NAELERRLAF ERSISRSWQT NLR AMQREL REHLASLALA SRKSGMSCDL FRGGILQAAE AAARRDLAGV PGCERESLRL LRQAADRIDS DAKRLEVYSS RIAE LEREV KDLRFSNTRL AESTTRVCAR SVDPVESLSV FPPVCDVCPR VSSVDPGDAL RSGLLFARQM LADRLVCKDM LHEID NRRK ELREREAALG FSHAALNCLR AVPADVYGMK RRKRQEENRE NHRRESESES LDDDVEEAET VRMRVSESAW SQDRDR LLV RQVELLRRLA GGEAKLRFPR TGAIEEAELG EEREKRRKKK KKRLEGYKEK GREDASYRLS PEVEALMQST EASLRDE VA DIKEKAEAET ERKAEKERLM WEVKKAREQA SREMRKQMEE MRKQMEKEKR ELVRRLEEEK LRHREALEAE EKREKERR R ERQKAEEAFW REREERRKKA EEDEERRRRE REEEDARLRA ERERRFQEEE EEREQRRQQR IREEDEERRM REERRRREE AEEEERRQRR KEEEERDAER RRHMLEKEEE DRRRKKEIEE ADKEARRRKQ AVEEEERRRK KRAEEEEEEE RRRKKRAEEE EEEERRRKK RAEEEEEEER RRKKRAEEEE EDRQRRLREA NESAQKEEDS VKNETASSPP HTDNISTTRQ EEPGREATAD A SDGQPPRS LGRLAKRNAS LATSIAPVKM AEVADLAENP EKSGTAASHI DEEDSRSVAA STSSGAGEGD LSDSETESVT NA SYSPRTA LKHKILDKYN SLANAFKYMD TRRVGSVTLH QFADFVDESG LDYPFSTVHS LYCQLSQPAD TLTEGLMYRN MDG DATLVQ LGRRLEDIYG NMGVPFEAEG IGPTGRVTRE AFYRVCTKAG LTAENSHALW NSIDLLGSGH LQMESLLLLL EDNV ELEEV KRVEEEIANM EGGFMSFFGQ TGPVPSPQPK HKRHESCGRP LALTRKLSLG FTEAEAAIDN VLEADSHWDV LFPLT RLAM IKQMQKTVYQ PGERIQEAQD NACPLYIVMT GSIQLLSPGL LMETVAEEKS APQLLFSEEM LEDKPNPFAV KALTAT KVF AYSREDFVRS VKHEQIKRTR DVATNMALIK IVPVLSKLTE VQARRLARTF GEETFEDGEA IFKEFDEPDN FYLVKKG EA AVVKYKKDPT TKEMEEVVFR IYQPGEYFGE ISILRDQTRT ATVKARGICT VLSLPKEDFN SQLRRFEAAF IGRAGRVY T AQGHVDKDKK PKKEKETLPT SVEEPEEPTD GASIEETSAD SQSREGLGQT IEDMLATEQT KLRDECMLLL EGVPVLKEL SLERRKEIVK AMSLECFYPN TPIILQGEES QNFYVLKTGT VAAELYVGPG KPFRKIKEYN STCYFGEMSI IKNEPRGCYI TAKSPVQLY SLTGEKFKEL LEDCYPAFQE YAQAQYEQET LKRQASSQLV SSEAVREEPT ADYGEIMNLL VQVPVINMLD E DALQQLTR AFKVECYQER EVIIHEGTPA EHFFIIFSGD VSVQKIVDGA PVEIVVLHAG EYLGEIAFLN EQPRTASCVA KS DVKLLSL TRSDFQVHLG ALQDAFLNQA ESKYAASKDE AIPDWLVEAG EQGALYDPSG SKKTEDEDEE PDGAFRGTVD IDK EKEMYN KKRGRKDSDT SRKKSDASAD KTGEAKASDA HRKKSGETPP GDGEEEAGKP GNDEVAEVAA DTNTVEKKKK KAKE KEKHG GESEETGGET KKKGKQSQAK RRMSLAGGAA LLAAAAAAKA AEGEPSPNEK EQEEKEETKA GEMEEKQDKE ETAEK EKKK EKKKKKKKDE DEDAEDKAAE EQRIDEFRDL VRGTLKNKYG SIAQAFQQID GNNSEIVDFD EFAAFVKDLR VQSLKK KDI QVLFDDLCQP LKGTLTVANL YRNIDKKDQL SKAEINLRWV EIFGGSRKAF KAVANLGGGD ECTEENFINV AAAAGVS EE NAKSIWTELD KAQTGQMHTK VICSYMAGEL TAEEAAAKEE ELASLGTRLW NFFGGSASDD ESASASSPTS QGDEQKIQ E ALKWEDMAIV QRDDHRTYPT CTKRLVDVLE KKEGFMNLQL LQLRYVASTM RRECLAKGVR VMEAGDEACM VFCAWGEFK ILQAGLFGES EVGVVCPDEC FGLSELINDS PLTASLVTEV DNSVLWVLER DTYNDLIRDM LEARRAVVPV IENFLKSVPI VKDMSETEI QNVARACKIE TFSPHQTVFR AGMAGDMFCF IFKGEITMLK PMGAGQEPIE LGRLQAGVYF GEMAVIKNQR R TASIIAAT DLTLFCLDKG SFERVLGRVK GKMIERAESL YKRQDVATTD PKEGEEGNAA ASSASAAGAA ESFGPGDHFE KK RTRKSSL ALLQYTKIRE AGPAEGESEK KSSEEDEERE ARWAMAQDPE IIHRPPPLSA APKKSALRTT SSKSVGIKNS VFF DEETLL PSPPSSDEGE

UniProtKB: Cyclic nucleotide-binding domain-containing protein

Macromolecule #15: myosin L, TGME49_291020

• Macromolecule: Name: myosin L, TGME49_291020 / type: protein_or_peptide / ID: 15 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 278.088031 KDa

Sequence

String:

MDASRKGRRR RPLSPLDSVD FSSVTSSACA SSELRYPAVP RPSDFAAFSG FPKSDSRSSS ASNHTSASVD ARTRLLRSSD ARQSLPVFS SVACLGRPNS GEASGKDTPE PGPSTNAASA FRPLTPNAAL AAQTGTPQSI TSAQRSAVLR GRMTPGGNGV A AKRIQIVT SAPKGEPQVA ASTPNHEHGR AKGDSALMDV IYGGGRRGVF APTPEDSSAF YIQLADLQQR QKLEASRARA KK IWLGPTA AKKEDSPGRL SIQAKRLSKA APEGDLQTVG KAAVYDQRHS RFGPQQEASE SPTSQSGSAD DFSALSGQAE SDT AVSDAE SPNPMSDMHS SFLLDQVDES LLRRSTSFLR CGGHVILRSH VEGSTFSSGA NASGTLRGRG AKGKGVKLWD LGTV FGVEG RRKLKASMAV TRAMLAAAKP EGRHRSAQVI DSNFLGRVLI QSTDSDPRLR DTAEDSGDRF HDRFAYKPDP RAHPN LSVD ARQEVVPVGC LLSGSPQDLE GKLGDLLYLS ASTPAAVVDT LRGRFYHDEY FTKCGNMLIS VFPPKNRERI FDTEFS EEN RWQYYAGEIQ DLRPHPFLLA RRALENVWLK RRNQFILTFG ELDAAQDNIQ KELFRYIYTF APRDGAKDQD GKRPDSA YS LMDEQNQRKE VLDAVDRLRQ AFSITTQDGG ISERCLDLVR AEFNSDNYLI GMSFVPNTAL EANVELISCA ARHTYYPL P VANICYAVLY GVHHQPDHPI LSQTQLKPVD ASFILKQLPP QPVLPYEEVQ FAAYLQFVQD LQKIGLNETE ITEFTRLIS AALVADSIEQ ERNEDEAIGG GQRTWNEEKT GGRGCHLPPV NTNPLFAGRD GVEVLESILG LVPESLRDFY QTEQIWQIRT LSLKLFSRL RWWLLGRIND ALRCRDIQFG RRTSIVLMAS TGLDLRRLHS SWSLLQLMRN YLDECFLNLF VSWGFTIEEA L YAREGVES PDVKYRSPRE VLEIIGGEQG IFYQLGRLHQ VFEGEFVTAS SLAAGNAAQQ VVEDVLRLSP SPDVFFTLPG YE AAREKAE QAEEQLARGR GGVKDEHQGP VFMRRRDPGE AAGGNLNRRN VTVYQKKDSI LGQFVVRHST GQARYDARDF VSV NAAAYT LSPDLYSVLS RTKLALLRRI MGAGNEADEL PHTLLRTDVV AQFQQLMNSL LLSDSPDPPE CHFIASVNST RIEL PSVAL ANNNATVTDD MWGRVSSFTR SRTFDPAKKL RDAFALQILG NRGENAETSD TSENDSPRSA TSVSPLQGPR KKRGN REEH VQVPSASSTV ATSTSDRDEV GDEDPKHKGR KGPPLYWKQK AVQVPGFMEI YTLEPTTSEF DLNPSIPSGK NTSIMA VLK EAEQRKPRRA REEEKSPLFD YAHVWGQLAD LQVLNYCTFQ HQGMTVRLTF AEFLRLYAVL VVPIVYPHNS VDDLLGS RK PKEAVIYLLQ TIKFPTSDYQ LGRSLVFFRK RTFLVLEMQR AAYLRRVMPA VATLCRAWPT FRARLFRTEM HWLAVRVQ S QARRIVSCMQ RKEMQKVINF LTGVVIFGMH IDKYRDSNLT QEQIRKMETK SRDKEVYCLR WAATTFLQAW WRGILARKR AGAMRQELLV TFASQIIKNT WRRYRAQEVL LSKVELNIIP AKAATRIQAQ VRRWICQRRY QRIRGLALAL LSIRRKGSKL YGLRVQLNY TPIYKRTTVV RDMLSMKNSD AVVRIQSMLR MAYVRRQYIT LRNAAMYCQA AAFTRIRRAE FLQARKAVIY I QRWWRTRK ALLELPIQHA LAPAPKLPKG SFSFLARRER TSAALLRGPL LASQGFFDSV TQRSGRHFLS LLDFQAVQDI RN IYPQTWA APLLRLLEQI QAESTKLQEV KTAVEKGAAL NLEQIEIGGQ HSLVLLHQRV RVDKQDFGQK VASRSTGMYE EFA IFRPTV YAWGWNDRGQ LGTLDTSMET DGLMCVGPLR FIDRQFRRYR DKKTGHLLHL LSHEIDYSYQ VTWIGAGLDH CIAF VGDGL VFAWGDNRVG QCGLGHRITT VFEPQLIQSL KEQSFMAVNG SVGSRHTAIV AADGQCVIFG SGAFVALDTI PPDSN FYTP CRLPLPDDAT ARMVSCGFGF NIVMCNREPG VYGWGRNDLG QLGLGTEKKS VRDLPTFIPL PRKPSAPLVV EKVATG PDF VVVCISPAKA CVYTWGVHLL TDPSAAASAP AAAVTGRGVR AAMNPFLKMQ AAKTAAPKTA AQAPKPPFRR LTKLRAV CE PTLMTHPLWR GRKIVDVACG LREVMVLTDL GFVFGFQSST VKWIEQEKPP EPEQTAATPT YSAFFKKKEV TPKEPEPA P KARVPSARFK PITGRFSLEP GLFSFRFCRP SRGIVKELRS SYCSVSCSTF WANSVKRTRR EVDEVIAATE SEVLKEQFS CTGDRLKFPP SIVAKAREEH KKIQEEQLMA HGQPPPQMKD FSPWPREMEW RQPMPDPRLV ARMKAEYRKE AVNGPSNLGS DSSK

UniProtKB: Myosin head (Motor domain) domain-containing protein

Macromolecule #16: PCR13, TGME49_284620

• Macromolecule: Name: PCR13, TGME49_284620 / type: protein_or_peptide / ID: 16 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 254.343016 KDa

Sequence

String:

MSRRVERLRR GEGLLSFGVV HDSLAVLGPT VPGVDDPKNA TPSKCSEWLT QSELQRAPVP RTSRWLPVTT SSADRFDRLR DISLKNEAD EEQLIPGVKL RLKDPGKRES SCQDDEPDAW HPASPSLIWE RNSTNVGTRS TANVFPFERI QATQASETRI L GGYTEQLT APFERVGATS EKFAYNACAT TLPYTGLWHD EVTGISDPTL DSKQLEALQK TPASRAAEAA LLRSQAAKVC PR AAKVLLE ELTRDVPDAP DAEGQADLQE RLFTKNKEYL SDDERVENFL LATVQPGLAL GVAQKAEKQD QRNRRRGQAA AEA KAKAIA SFWESRKPGS EARKRPPAPE PWQRRDALIQ KEVEKSRASL SGPELAIHRR HSNKRMIIYA ERFFGDLGAA SEGA EADPL RLIRGNSATV AREISREGRQ QANAESLIAR LEMRLTGGTR GHEMGRKARA THSRISRGDS VPRQLIAERT EGQFD GRRG VLDGVGGSRR GSGQPWVLED YLGVRRAMRL AANLERDQER EAEILGIPIV RRDKPKNTHA GAPEMGAEHQ ASRTPP TPS APTKPVSVPP PTPLLSPCPS AASPAGGEKN DAASASDSKA ADTGKAPPKP PPGSKPMPGP PPKKTPPPPG AGKKGPS PP KPKMKLAFQK VSTHTASIPT ELPEVPSNAV SPRVENLSKA SGAAVETKLS EKAKRSSESE DESEDGEADL PLPLLLPQ S NEASAALSSA NALDAIRRTR FPTNLRPKPV YDSIGRVILP PRRKADASGT ASGRPEDCVD NDNAAGIADP SGMVSYKLV YDNPVYTGPS YEVMAEYQPE LEPGAKPLGP GAETLIYEPA ASRTASSGSY GTHPVLDKKR RRQEAEGGER RRGREERSLL PRPTQFDPV TRYPHDESSR ASLSLLGVND DEWVSSGISR LANCVADLTT APSAVGVLEM LGEQGKTGKP VAQAAVWPAM L SEEGALGQ NVDTLKQVEC VVDGGFPFLK LEAMPGTSAK TSRQHAGLSL WELHSSAKNN RRHELVDSFG NVRVAPFASR IF VPVRLMG KDSKFTKREE ALQARYAYPE GQFSPGYYVK AADIDEKRQA ILQARPPRMS VFEFQEKMRQ LKLAKAREET ERA ERDQKE VDARLALIAE KGFQRSKGNA VAGHANKGGV DHAVAGEGTK GLPTFADLDA DLLKERYRAI KKKNAHDKSL QQIK SLIAE KSGKQKCKLK VMGRHRHPKG TNKEIELERQ RKKERILRML ADPHPDVNAA LLLAEIKEIE KERGSESDEF NSIST QYTF DTFTVSSYET VEDAFPHYTF DYPRYDPCPE TFSALQKARL NYSGVLLVSE DFRHTSAKAR ARLAVASTLP TGGYLH HLM YVEVRDSVVF FYSDTKFSGI AGGKTVLHAF AKGLDASQLV VDGTEDWTLL FGVCVDALTQ VDVVTVEPFW SPAAELH YV AVRISGRNAN FLPGAPPRDL ISTATEGSSK KTKKTAEQRI AQEEEVTLFL SADTEAETLL WHRLLKRRVA FAKYASAL A QETRGDRPAL SIAEFCLSGV NTAVLSMKGV AFNPTLDQVL FSNLEEEKID YLDMSCRNLG DADIADLVDI LPFFVECVD LSGNQIQADD PVQVCKLMNK LHATSVDLSD NPLGQRATGG HLLGTLLTQA FLSRLDMNRC RFGAEAAKAF RENVAAIGRP VERPIAVSL SGNDLSATDV VSMVTSIKSK IPGVKSISVA GNMALTAEEF WAAVKASFHK VDVSILNFDF DQRPSPSLST P VSGDMNQL KGLPLTLSGR LFGAGWKICG DRYTWSPAAQ GGSQSPETTM GYHQRVADAD GGQAVPLVYF ELRGPVLIWS DV PARSVGW LCPEGQKLTS SQQRPTEPLP KDPMAASRTI CAVLLYRVHV DFDAHPMVLI VEALELCESN DPNRIFPSVN LPP LDELPE QQTVSYVLRG PTDAVTLEWA RILHARLAGM YYVRTEGRSL HQLSPSALRF FESGVGTILD FGGCPVSPMG LKSI FYFLC KYTWLKSLIM CNMHLSNLHL KFLSKEAWQL YDLDLLDLSF NDFTDASMEL LTTVLCFRSC KRLVLDQNGF SDCEN AADL VLRVGCGHEV TCLCLNGCSL GDRFAEALAI GLKTLAPNRA DSCLAVVELQ SNCIHAVALR NLVNALVGVL PTIESV KVS GNADVEAGKM SLPFADFKNT FQGIPARKQR VIGPRKRLIK WGARPTEVGS EGHDLLVADE ARNALAHTEG SPVPDQP AG GADGSEGVMG ADKAGSAEGA EMGEEDGKAE QDAGAEKAVT PEESNSDDEK SDKRGSGEGS GKSDDGERSE ESDHGEGS K NNSDAERSEK SD

UniProtKB: Leucine rich repeat-containing protein

Macromolecule #17: PCR15, TGME49_232560

• Macromolecule: Name: PCR15, TGME49_232560 / type: protein_or_peptide / ID: 17 / Number of copies: 3 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 200.557844 KDa

Sequence

String:

MASSTSQAPS SRSSWKRHFE TALQRATASA DKFLQCSSRV LLSSRENAPS VRGPSSCTDS VSPLSVVSFP RQCGLPSGDA FFRGRPPGN AAQGGSSCGK NASFGYPLVS GTKEASVSAV HSDFISYEET KALARFKGRG REYIPPKLTK DGGVLFGGSG S KVTSQMKR QLECVTVVTK REKVNNILDL TTEASQRTLR NTDLPLCQEP DLGSLYTKQL KEIENAKECL KDIHVQKPWQ TW PPKEETL TLVHHTASRN PLSPFTELHD PIENREYADS WRCPAPPSLG SGFRYSEQSG LNSPTPSDAM ASWFKHKKAD ETH DDSAQE LSNFVALLHT ISEESMKLPP PKKKVQTPTN PLHPLLIRDT AGSADASGAP QPVVLTVVRQ EGLKAKLEAI DGDA VSARA APAAKRKNEK EASVQTADPP PQLKPTEAKS KSPPTSAPKG PKISRTLTDL AVSESSTSES EASPRLVPLS QAMEP VAIV PAELSAAAPA AATSSGVTEL GTRSRTPRRA SRMELRRKSI QDIERAIAER KARDRYNEYL DLVNGLPRRC LPQLNG DEE DDSSSRNLRQ SELSVDLPPW LYFLDTHTTL PEPVGMSTVV PSVMVPRLQD AAFEEDELSA EDLPGFAAFE QLTNLFA PG FKEFLYPEYD PSSASPMAIP MIQLVDPDKV NVWQEVAENA LHEEWELTQR EWGTPLQAEI EAYQKLERKL FARRKKQA R QRVIAERKKD INQDVDLVQR RLRQDIQQQY DEFVSTEAPL QQSMQHELNK GLRAIGSCRG GRRAWAVFNR SIRSEATQR REASPSYKRR LEEDAFGFVP PPAVGRRCGR QATRAGRGRM PGGHARASET VSSQKGEDKS TAMGDRGIKG AGGAGQRREP IEDESSLTS EEMENQAVDL LRQRDLDRML QDAASQIRPD FGKRVASPPA KVEQTRLSSP ADGSLQQHDA DNGFHVRMLQ Q RMRASEVA TKIENTSSLV AYGEVPSQVK ELFGHGVNRL PNSAGSVSEN AFVGLLGSHI PHEVGPSTVS DSKTRGSSAG RL ICQASQS KSASSQLCIG GSDPLNVDGN ERRDSRSGIA QGLERVPTPT EADSDFEEAN RDEDAFCPLP TLGRHIDASD YER QPEPME VITSKDAIDV TLHSANLRDK QSCRWTQVPS LPCDEHSFHE EPTQEELQVD CHVSLNRHDS HDEAQTSSPS CRSG KGRLL CSWSAIGDSG NDENEGKCRS MCESGHAVRC VGARRSGRCQ ASRLSKIDKE CDADKCGFDA TKDGTSFSPR SFQTL INEE KQGQIPHAKG KRFGETASEL DEPGTSSVAH EGVFDSPSSY ISTISPRSPR GQQALVKTDS FQSVPRKHAF SKEDVR QCP GQRQGSPVPR DLGKLEHSLQ ESSEVVEVTR RGTTNAPGEE QGVTSRSSVT TESDFNHQKQ LLYKQKPHLR SLFRPLS AE RVRRPSKFPW DDEVIPTCRA QGETPVLSLG EDPSYAKDNL CNFNSSPRTR EKSRTERGAS VTNSFCGTSG RETPPGQQ E WPCIHHSPQR QSQPFLRRGC GEGGGRYHKR HESNRKRPPV RNELTVSPRN QTQLEVCNSA ETVSGLKLSP FKEEIGVGN YRKSSPRDAA TVFDIQSSRS ASDLCTVSIS GGSADSMIDF PVLWPSIRSL YKSSECSRRC RKSPSDRNPL VCIDEANRWD GTQDRSKPH EFLKKGSGRC AGQSKQKEES SATSRPTTPR HASTAGGASP RVEPEDILSA TGEGRTRVCM RFEKGERNST P GSQSVATE RASSGSLKRS SSEDAGRRAG EKDKVPYTRL VDLSSHFQRP RVRWGNPISQ AFCE

UniProtKB: Uncharacterized protein

Macromolecule #18: MLC4, TGME49_294390

• Macromolecule: Name: MLC4, TGME49_294390 / type: protein_or_peptide / ID: 18 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 19.71673 KDa

Sequence

String:

METSKQKEKT SSQTCSFAFL RRKPLIIRHI DALKVESAFC EISGASRTIQ RKEAVANLAR YLGLAPSEQE VDTFFHRVNG RCDLAAFGR FCSLLVHDED KAANFRELFL VYDKEHTGKL KKRVVKMIFC NLGEPLSAAE WRSFEGIFDL ADKKDDDLVP Y EPIVRKLL DLPQ

UniProtKB: Putative myosin light chain MLC4

Macromolecule #19: Calmodulin

• Macromolecule: Name: Calmodulin / type: protein_or_peptide / ID: 19 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 19.070646 KDa

Sequence

String:

MPPRGSASAG AKPSALDKVP PRLRVHAAEL ETIFTRIDAD GDGKLSEADV MAFMKDRVGF PLEPDQVKAL LVELRGPGAA ADPGKGAAS PVDFPTFLCF VDNQLMKPLP EVLREVFQYV DTDRDGKLTA GELQGFAARL GLNLSEAGAA HLLNRGQNET C ANFEEFCG LVQASLPSAN K

UniProtKB: Calmodulin

Macromolecule #20: Calmodulin

• Macromolecule: Name: Calmodulin / type: protein_or_peptide / ID: 20 / Number of copies: 4 / Enantiomer: LEVO
• Source (natural): Organism: Toxoplasma gondii (eukaryote)
• Molecular weight: Theoretical: 16.819641 KDa

Sequence

String:

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TLMARKMKDT DTEEELIEA FKVFDRDGNG FISAAELRHV MTNLGEKLTD EEVDEMIREA DVDGDGQINY EEFVKMMMAK

UniProtKB: Calmodulin

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: filament

Sample preparation

• Buffer: pH: 7.5
• Vitrification: Cryogen name: ETHANE

Electron microscopy

• Microscope: TFS KRIOS
• Image recording: Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Ų
• Electron beam: Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 25.0 µm / Nominal defocus min: 5.0 µm
• Experimental equipment: Model: Titan Krios / Image courtesy: FEI Company

Image processing

• CTF correction: Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
• Startup model: Type of model: INSILICO MODEL
• Final reconstruction: Resolution.type: BY AUTHOR / Resolution: 3.54 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 131707
• Initial angle assignment: Type: MAXIMUM LIKELIHOOD
• Final angle assignment: Type: MAXIMUM LIKELIHOOD