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- PDB-9y9z: Cryo-EM structure of conoid fiber from Toxoplasma gondii (24-nm r... -

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Basic information

Entry
Database: PDB / ID: 9y9z
TitleCryo-EM structure of conoid fiber from Toxoplasma gondii (24-nm repeat)
Components
  • CF1, TGME49_222350
  • CF2, TGME49_258090
  • CF3, TGME49_255895
  • CF4, TGME49_246720
  • CF5, TGME49_297180
  • CF6, TGME49_245640
  • CIP3, TGME49_225020
  • CPH1, TGME49_266630
  • DCX, TGME49_256030
  • Microtubule associated protein SPM1
  • PRP, TGME49_291880
  • Spindle assembly abnormal protein 6 N-terminal domain-containing protein
  • Tubulin alpha chain
  • Tubulin beta chain
KeywordsSTRUCTURAL PROTEIN / Tubulin / Microtubule / Microtubule Inner Protein / Microtubule-associated Protein / Toxoplasma gondii / conoid / conoid fiber
Function / homology
Function and homology information


condensin complex / mitotic chromosome condensation / microtubule polymerization / microtubule-based process / condensed chromosome / tubulin binding / structural constituent of cytoskeleton / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule ...condensin complex / mitotic chromosome condensation / microtubule polymerization / microtubule-based process / condensed chromosome / tubulin binding / structural constituent of cytoskeleton / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cytoskeleton / intracellular signal transduction / hydrolase activity / GTPase activity / chromatin binding / chromatin / GTP binding / metal ion binding / cytoplasm
Similarity search - Function
P25-alpha / p25-alpha / Stabilizer of axonemal microtubules 1/2 / Spindle assembly abnormal protein 6, N-terminal / SAS-6, N-terminal domain superfamily / Doublecortin / Centriolar protein SAS N-terminal domain / Doublecortin domain profile. / Domain in the Doublecortin (DCX) gene product / Doublecortin domain ...P25-alpha / p25-alpha / Stabilizer of axonemal microtubules 1/2 / Spindle assembly abnormal protein 6, N-terminal / SAS-6, N-terminal domain superfamily / Doublecortin / Centriolar protein SAS N-terminal domain / Doublecortin domain profile. / Domain in the Doublecortin (DCX) gene product / Doublecortin domain / Doublecortin domain superfamily / CRAL-TRIO lipid binding domain superfamily / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Ankyrin repeat-containing domain superfamily
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / EF hand protein / Uncharacterized protein / Uncharacterized protein / Microtubule associated protein SPM1 / Ankyrin repeat-containing protein / Spindle assembly abnormal protein 6 N-terminal domain-containing protein / Doublecortin domain-containing protein / Tubulin beta chain ...GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / EF hand protein / Uncharacterized protein / Uncharacterized protein / Microtubule associated protein SPM1 / Ankyrin repeat-containing protein / Spindle assembly abnormal protein 6 N-terminal domain-containing protein / Doublecortin domain-containing protein / Tubulin beta chain / Tubulin alpha chain / Bax inhibitor 1, related protein / CRAL/TRIO domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein
Similarity search - Component
Biological speciesToxoplasma gondii (eukaryote)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.14 Å
AuthorsZeng, J. / Zhang, R.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI179885 United States
CitationJournal: Nat Struct Mol Biol / Year: 2025
Title: Atomic models of the Toxoplasma cell invasion machinery.
Authors: Jianwei Zeng / Yong Fu / Pengge Qian / Wei Huang / Qingwei Niu / Wandy L Beatty / Alan Brown / L David Sibley / Rui Zhang /
Abstract: Apicomplexan parasites, responsible for toxoplasmosis, cryptosporidiosis and malaria, invade host cells through a unique gliding motility mechanism powered by actomyosin motors and a dynamic ...Apicomplexan parasites, responsible for toxoplasmosis, cryptosporidiosis and malaria, invade host cells through a unique gliding motility mechanism powered by actomyosin motors and a dynamic organelle called the conoid. Here, using cryo-electron microscopy, we determined structures of four essential complexes of the Toxoplasma gondii conoid: the preconoidal P2 ring, tubulin-based conoid fibers, and the subpellicular and intraconoidal microtubules. Our analysis identified 40 distinct conoid proteins, several of which are essential for parasite lytic growth, as revealed through genetic disruption studies. Comparative analysis of the tubulin-containing complexes sheds light on their functional specialization by microtubule-associated proteins, while the structure of the preconoidal ring pinpoints the site of actin polymerization and initial translocation, enhancing our mechanistic understanding of gliding motility and, therefore, parasite invasion.
History
DepositionSep 15, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 3, 2025Provider: repository / Type: Initial release
Revision 1.0Dec 3, 2025Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
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Revision 1.1Dec 17, 2025Data content type: EM metadata / Data content type: EM metadata / EM metadata / Group: Database references / Experimental summary / Data content type: EM metadata / EM metadata / EM metadata / Category: citation / citation_author / em_admin
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Revision 1.2Dec 24, 2025Group: Data collection / Database references / Category: citation / em_admin
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: CF2, TGME49_258090
B: CF2, TGME49_258090
C: CF2, TGME49_258090
D: CF2, TGME49_258090
E: CF2, TGME49_258090
F: CF2, TGME49_258090
G: CF2, TGME49_258090
H: CF2, TGME49_258090
I: CF2, TGME49_258090
J: CF2, TGME49_258090
K: CF2, TGME49_258090
L: CF2, TGME49_258090
M: CF2, TGME49_258090
N: CF2, TGME49_258090
O: CF2, TGME49_258090
P: CF2, TGME49_258090
Q: CF2, TGME49_258090
R: CF2, TGME49_258090
S: CF2, TGME49_258090
T: CF2, TGME49_258090
U: CF2, TGME49_258090
V: CF2, TGME49_258090
W: CF2, TGME49_258090
X: CF2, TGME49_258090
Y: CF2, TGME49_258090
Z: CF2, TGME49_258090
1: CF2, TGME49_258090
10: CF1, TGME49_222350
11: CF1, TGME49_222350
12: CF1, TGME49_222350
13: CF1, TGME49_222350
14: CF1, TGME49_222350
15: CF1, TGME49_222350
16: CF1, TGME49_222350
1A: Tubulin alpha chain
1B: Tubulin beta chain
1C: Tubulin alpha chain
1D: Tubulin beta chain
1E: Tubulin alpha chain
1F: Tubulin beta chain
1G: Tubulin alpha chain
1H: Tubulin beta chain
1I: Tubulin alpha chain
1J: Tubulin beta chain
1K: Tubulin alpha chain
1L: Tubulin beta chain
1M: Tubulin alpha chain
1N: Tubulin beta chain
2: CF2, TGME49_258090
20: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
21: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
22: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
23: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
24: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
25: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
26: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
27: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
28: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
29: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
2A: Tubulin alpha chain
2B: Tubulin beta chain
2C: Tubulin alpha chain
2D: Tubulin beta chain
2E: Tubulin alpha chain
2F: Tubulin beta chain
2G: Tubulin alpha chain
2H: Tubulin beta chain
2I: Tubulin alpha chain
2J: Tubulin beta chain
2K: Tubulin alpha chain
2L: Tubulin beta chain
2M: Tubulin alpha chain
3: CF1, TGME49_222350
30: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
31: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
32: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
33: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
35: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
36: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
37: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
38: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
39: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
3A: Tubulin alpha chain
3B: Tubulin beta chain
3C: Tubulin alpha chain
3D: Tubulin beta chain
3E: Tubulin alpha chain
3F: Tubulin beta chain
3G: Tubulin alpha chain
3H: Tubulin beta chain
3I: Tubulin alpha chain
3J: Tubulin beta chain
3K: Tubulin alpha chain
3L: Tubulin beta chain
3M: Tubulin alpha chain
4: CF1, TGME49_222350
40: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
42: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
43: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
44: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
45: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
46: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
47: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
48: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
49: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
4A: Tubulin alpha chain
4B: Tubulin beta chain
4C: Tubulin alpha chain
4D: Tubulin beta chain
4E: Tubulin alpha chain
4F: Tubulin beta chain
4G: Tubulin alpha chain
4H: Tubulin beta chain
4I: Tubulin alpha chain
4J: Tubulin beta chain
4K: Tubulin alpha chain
4L: Tubulin beta chain
4M: Tubulin alpha chain
5: CF1, TGME49_222350
50: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
51: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
52: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
53: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
54: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
55: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
56: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
57: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
58: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
59: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
5A: Tubulin alpha chain
5B: Tubulin beta chain
5C: Tubulin alpha chain
5D: Tubulin beta chain
5E: Tubulin alpha chain
5F: Tubulin beta chain
5G: Tubulin alpha chain
5H: Tubulin beta chain
5I: Tubulin alpha chain
5J: Tubulin beta chain
5K: Tubulin alpha chain
5L: Tubulin beta chain
5M: Tubulin alpha chain
5N: Tubulin beta chain
6: CF1, TGME49_222350
60: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
61: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
62: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
63: CIP3, TGME49_225020
64: CIP3, TGME49_225020
65: CIP3, TGME49_225020
66: CIP3, TGME49_225020
67: CIP3, TGME49_225020
68: CIP3, TGME49_225020
69: CIP3, TGME49_225020
6A: Tubulin alpha chain
6B: Tubulin beta chain
6C: Tubulin alpha chain
6D: Tubulin beta chain
6E: Tubulin alpha chain
6F: Tubulin beta chain
6G: Tubulin alpha chain
6H: Tubulin beta chain
6I: Tubulin alpha chain
6J: Tubulin beta chain
6K: Tubulin alpha chain
6L: Tubulin beta chain
6M: Tubulin alpha chain
6N: Tubulin beta chain
7: CF1, TGME49_222350
71: CF6, TGME49_245640
72: CF6, TGME49_245640
73: CF6, TGME49_245640
75: CF6, TGME49_245640
76: CF6, TGME49_245640
77: CF6, TGME49_245640
78: CF6, TGME49_245640
79: CF6, TGME49_245640
7A: Tubulin alpha chain
7B: Tubulin beta chain
7C: Tubulin alpha chain
7D: Tubulin beta chain
7E: Tubulin alpha chain
7F: Tubulin beta chain
7G: Tubulin alpha chain
7H: Tubulin beta chain
7I: Tubulin alpha chain
7J: Tubulin beta chain
7K: Tubulin alpha chain
7L: Tubulin beta chain
7M: Tubulin alpha chain
8: CF1, TGME49_222350
80: CF6, TGME49_245640
82: CF6, TGME49_245640
83: CF6, TGME49_245640
84: CF6, TGME49_245640
85: CF6, TGME49_245640
86: CF6, TGME49_245640
87: CF6, TGME49_245640
89: CF6, TGME49_245640
8A: Tubulin alpha chain
8B: Tubulin beta chain
8C: Tubulin alpha chain
8D: Tubulin beta chain
8E: Tubulin alpha chain
8F: Tubulin beta chain
8G: Tubulin alpha chain
8H: Tubulin beta chain
8I: Tubulin alpha chain
8J: Tubulin beta chain
8K: Tubulin alpha chain
8L: Tubulin beta chain
8M: Tubulin alpha chain
9: CF1, TGME49_222350
90: CF6, TGME49_245640
91: CF6, TGME49_245640
92: CF6, TGME49_245640
93: CF6, TGME49_245640
94: CF6, TGME49_245640
95: CF6, TGME49_245640
96: CF6, TGME49_245640
97: CF6, TGME49_245640
9A: Tubulin alpha chain
9B: Tubulin beta chain
9C: Tubulin alpha chain
9D: Tubulin beta chain
9E: Tubulin alpha chain
9F: Tubulin beta chain
9G: Tubulin alpha chain
9H: Tubulin beta chain
9I: Tubulin alpha chain
9J: Tubulin beta chain
9K: Tubulin alpha chain
9L: Tubulin beta chain
9M: Tubulin alpha chain
A1: CF4, TGME49_246720
A2: CF4, TGME49_246720
A3: CF4, TGME49_246720
A4: CF4, TGME49_246720
A5: CF4, TGME49_246720
A6: CF4, TGME49_246720
A7: CF4, TGME49_246720
A8: CF4, TGME49_246720
A9: CF4, TGME49_246720
B1: CF3, TGME49_255895
B2: CF3, TGME49_255895
B3: CF3, TGME49_255895
B4: CF3, TGME49_255895
B5: CF3, TGME49_255895
B6: CF3, TGME49_255895
B7: CF3, TGME49_255895
C0: CPH1, TGME49_266630
C1: Microtubule associated protein SPM1
C2: Microtubule associated protein SPM1
C3: Microtubule associated protein SPM1
C4: Microtubule associated protein SPM1
C5: Microtubule associated protein SPM1
C6: CPH1, TGME49_266630
C7: CPH1, TGME49_266630
C8: CPH1, TGME49_266630
C9: CPH1, TGME49_266630
D0: CPH1, TGME49_266630
D1: CPH1, TGME49_266630
D2: CPH1, TGME49_266630
D3: CPH1, TGME49_266630
D4: CPH1, TGME49_266630
D5: CPH1, TGME49_266630
D6: CPH1, TGME49_266630
D7: CPH1, TGME49_266630
D8: CPH1, TGME49_266630
D9: CPH1, TGME49_266630
E0: CPH1, TGME49_266630
E1: CPH1, TGME49_266630
E2: CPH1, TGME49_266630
E3: CPH1, TGME49_266630
E4: CPH1, TGME49_266630
E5: CPH1, TGME49_266630
E6: CPH1, TGME49_266630
E7: CPH1, TGME49_266630
E8: CPH1, TGME49_266630
E9: CPH1, TGME49_266630
F0: PRP, TGME49_291880
F1: CPH1, TGME49_266630
F2: CPH1, TGME49_266630
F3: CPH1, TGME49_266630
F5: PRP, TGME49_291880
F6: PRP, TGME49_291880
F7: PRP, TGME49_291880
F8: PRP, TGME49_291880
F9: PRP, TGME49_291880
G0: PRP, TGME49_291880
G1: PRP, TGME49_291880
G2: PRP, TGME49_291880
G3: PRP, TGME49_291880
G4: PRP, TGME49_291880
G5: PRP, TGME49_291880
G6: PRP, TGME49_291880
G7: PRP, TGME49_291880
G8: PRP, TGME49_291880
G9: PRP, TGME49_291880
H0: PRP, TGME49_291880
H1: PRP, TGME49_291880
H2: PRP, TGME49_291880
H3: PRP, TGME49_291880
H4: PRP, TGME49_291880
H5: PRP, TGME49_291880
H6: PRP, TGME49_291880
H7: PRP, TGME49_291880
H8: PRP, TGME49_291880
H9: PRP, TGME49_291880
I1: PRP, TGME49_291880
I2: PRP, TGME49_291880
I3: PRP, TGME49_291880
I4: PRP, TGME49_291880
I5: PRP, TGME49_291880
I6: PRP, TGME49_291880
I7: PRP, TGME49_291880
I8: PRP, TGME49_291880
I9: PRP, TGME49_291880
J1: CF5, TGME49_297180
J2: CF5, TGME49_297180
J3: CF5, TGME49_297180
J4: CF5, TGME49_297180
J5: CF5, TGME49_297180
J6: CF5, TGME49_297180
J7: CF5, TGME49_297180
K0: DCX, TGME49_256030
K1: DCX, TGME49_256030
K2: DCX, TGME49_256030
K3: DCX, TGME49_256030
K4: DCX, TGME49_256030
K5: DCX, TGME49_256030
K6: DCX, TGME49_256030
K7: DCX, TGME49_256030
K8: DCX, TGME49_256030
K9: DCX, TGME49_256030
L0: DCX, TGME49_256030
L1: DCX, TGME49_256030
L2: DCX, TGME49_256030
L3: DCX, TGME49_256030
L4: DCX, TGME49_256030
L5: DCX, TGME49_256030
L6: DCX, TGME49_256030
L7: DCX, TGME49_256030
L8: DCX, TGME49_256030
L9: DCX, TGME49_256030
M1: DCX, TGME49_256030
M2: DCX, TGME49_256030
M3: DCX, TGME49_256030
M4: DCX, TGME49_256030
M5: DCX, TGME49_256030
M6: DCX, TGME49_256030
M7: DCX, TGME49_256030
a: CF2, TGME49_258090
b: CF2, TGME49_258090
c: CF2, TGME49_258090
d: CF2, TGME49_258090
e: CF2, TGME49_258090
f: CF2, TGME49_258090
g: CF2, TGME49_258090
h: CF2, TGME49_258090
i: CF2, TGME49_258090
j: CF2, TGME49_258090
k: CF2, TGME49_258090
l: CF2, TGME49_258090
m: CF2, TGME49_258090
n: CF2, TGME49_258090
o: CF2, TGME49_258090
p: CF2, TGME49_258090
q: CF2, TGME49_258090
r: CF2, TGME49_258090
s: CF2, TGME49_258090
t: CF2, TGME49_258090
u: CF2, TGME49_258090
v: CF2, TGME49_258090
w: CF2, TGME49_258090
x: CF2, TGME49_258090
y: CF2, TGME49_258090
z: CF2, TGME49_258090
hetero molecules


Theoretical massNumber of molelcules
Total (without water)22,934,895552
Polymers22,875,360378
Non-polymers59,535174
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Protein , 14 types, 378 molecules ABCDEFGHIJKLMNOPQRSTUVWXYZ12ab...

#1: Protein ...
CF2, TGME49_258090


Mass: 106251.133 Da / Num. of mol.: 54 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8GBZ2
#2: Protein
CF1, TGME49_222350


Mass: 47976.344 Da / Num. of mol.: 14 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8GJP0
#3: Protein ...
Tubulin alpha chain / Alpha-tubulin / alpha tubulin TUBA1 / TGME49_316400


Mass: 50166.645 Da / Num. of mol.: 63 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: P10873
#4: Protein ...
Tubulin beta chain / beta tubulin / TGME49_266960


Mass: 50119.121 Da / Num. of mol.: 57 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: I7BFC9
#5: Protein ...
Spindle assembly abnormal protein 6 N-terminal domain-containing protein / SAS6L / TGME49_301420


Mass: 29529.971 Da / Num. of mol.: 41 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A7J6KDX1
#6: Protein
CIP3, TGME49_225020


Mass: 114592.602 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8G5Q1
#7: Protein ...
CF6, TGME49_245640


Mass: 46600.648 Da / Num. of mol.: 24 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A086KRX4
#8: Protein
CF4, TGME49_246720 / EF hand protein


Mass: 56022.809 Da / Num. of mol.: 9 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A086KR31
#9: Protein
CF3, TGME49_255895 / Bax inhibitor 1 / related protein


Mass: 62988.641 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8F3E8
#10: Protein ...
CPH1, TGME49_266630 / Ankyrin repeat-containing protein


Mass: 67410.492 Da / Num. of mol.: 28 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A7J6KB56
#11: Protein
Microtubule associated protein SPM1 / SPM1 / TGME49_263520


Mass: 38845.750 Da / Num. of mol.: 5 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A7J6K285
#12: Protein ...
PRP, TGME49_291880


Mass: 80452.414 Da / Num. of mol.: 35 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: A0A151HK62
#13: Protein
CF5, TGME49_297180 / CRAL/TRIO domain-containing protein


Mass: 97829.719 Da / Num. of mol.: 7 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: S8FEB8
#14: Protein ...
DCX, TGME49_256030 / Doublecortin domain-containing protein / TgDCX


Mass: 29261.402 Da / Num. of mol.: 27 / Source method: isolated from a natural source / Source: (natural) Toxoplasma gondii (eukaryote) / References: UniProt: B6KAS6

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Non-polymers , 3 types, 174 molecules

#15: Chemical...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 63 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Feature type: SUBJECT OF INVESTIGATION / Comment: GTP, energy-carrying molecule*YM
#16: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 54 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#17: Chemical...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 57 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Feature type: SUBJECT OF INVESTIGATION / Comment: GDP, energy-carrying molecule*YM

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: conoid fiber from Toxoplasma gondii (24-nm repeat) / Type: COMPLEX / Entity ID: #1-#14 / Source: NATURAL
Molecular weightExperimental value: NO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 25000 nm / Nominal defocus min: 5000 nm
Image recordingElectron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

EM software
IDNameCategory
1cryoSPARCparticle selection
13cryoSPARC3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.14 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 95409 / Symmetry type: POINT

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