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- EMDB-72715: Cryo-EM structure of conoid fiber from Toxoplasma gondii (24-nm r... -

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Basic information

Entry
Database: EMDB / ID: EMD-72715
TitleCryo-EM structure of conoid fiber from Toxoplasma gondii (24-nm repeat)
Map data
Sample
  • Complex: conoid fiber from Toxoplasma gondii (24-nm repeat)
    • Protein or peptide: x 14 types
  • Ligand: x 3 types
KeywordsTubulin / Microtubule / Microtubule Inner Protein / Microtubule-associated Protein / Toxoplasma gondii / conoid / conoid fiber / STRUCTURAL PROTEIN
Function / homology
Function and homology information


condensin complex / mitotic chromosome condensation / microtubule polymerization / microtubule-based process / condensed chromosome / tubulin binding / structural constituent of cytoskeleton / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule ...condensin complex / mitotic chromosome condensation / microtubule polymerization / microtubule-based process / condensed chromosome / tubulin binding / structural constituent of cytoskeleton / microtubule binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / cytoskeleton / intracellular signal transduction / hydrolase activity / GTPase activity / chromatin binding / chromatin / GTP binding / metal ion binding / cytoplasm
Similarity search - Function
P25-alpha / p25-alpha / Stabilizer of axonemal microtubules 1/2 / Spindle assembly abnormal protein 6, N-terminal / SAS-6, N-terminal domain superfamily / Doublecortin / Centriolar protein SAS N-terminal domain / Doublecortin domain profile. / Domain in the Doublecortin (DCX) gene product / Doublecortin domain ...P25-alpha / p25-alpha / Stabilizer of axonemal microtubules 1/2 / Spindle assembly abnormal protein 6, N-terminal / SAS-6, N-terminal domain superfamily / Doublecortin / Centriolar protein SAS N-terminal domain / Doublecortin domain profile. / Domain in the Doublecortin (DCX) gene product / Doublecortin domain / Doublecortin domain superfamily / CRAL-TRIO lipid binding domain superfamily / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily / Ankyrin repeat-containing domain superfamily
Similarity search - Domain/homology
EF hand protein / Uncharacterized protein / Uncharacterized protein / Microtubule associated protein SPM1 / Ankyrin repeat-containing protein / Spindle assembly abnormal protein 6 N-terminal domain-containing protein / Doublecortin domain-containing protein / Tubulin beta chain / Tubulin alpha chain / Bax inhibitor 1, related protein ...EF hand protein / Uncharacterized protein / Uncharacterized protein / Microtubule associated protein SPM1 / Ankyrin repeat-containing protein / Spindle assembly abnormal protein 6 N-terminal domain-containing protein / Doublecortin domain-containing protein / Tubulin beta chain / Tubulin alpha chain / Bax inhibitor 1, related protein / CRAL/TRIO domain-containing protein / Uncharacterized protein / Uncharacterized protein / Uncharacterized protein
Similarity search - Component
Biological speciesToxoplasma gondii (eukaryote)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.14 Å
AuthorsZeng J / Zhang R
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01AI179885 United States
CitationJournal: Nat Struct Mol Biol / Year: 2025
Title: Atomic models of the Toxoplasma cell invasion machinery.
Authors: Jianwei Zeng / Yong Fu / Pengge Qian / Wei Huang / Qingwei Niu / Wandy L Beatty / Alan Brown / L David Sibley / Rui Zhang /
Abstract: Apicomplexan parasites, responsible for toxoplasmosis, cryptosporidiosis and malaria, invade host cells through a unique gliding motility mechanism powered by actomyosin motors and a dynamic ...Apicomplexan parasites, responsible for toxoplasmosis, cryptosporidiosis and malaria, invade host cells through a unique gliding motility mechanism powered by actomyosin motors and a dynamic organelle called the conoid. Here, using cryo-electron microscopy, we determined structures of four essential complexes of the Toxoplasma gondii conoid: the preconoidal P2 ring, tubulin-based conoid fibers, and the subpellicular and intraconoidal microtubules. Our analysis identified 40 distinct conoid proteins, several of which are essential for parasite lytic growth, as revealed through genetic disruption studies. Comparative analysis of the tubulin-containing complexes sheds light on their functional specialization by microtubule-associated proteins, while the structure of the preconoidal ring pinpoints the site of actin polymerization and initial translocation, enhancing our mechanistic understanding of gliding motility and, therefore, parasite invasion.
History
DepositionSep 15, 2025-
Header (metadata) releaseDec 3, 2025-
Map releaseDec 3, 2025-
UpdateDec 24, 2025-
Current statusDec 24, 2025Processing site: RCSB / Status: Released

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Structure visualization

Supplemental images

emd_72715.png

Downloads & links

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Map

FileDownload / File: emd_72715.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

Image control

Size
Brightness
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.34 Å/pix.
x 512 pix.
= 686.08 Å		1.34 Å/pix.
x 512 pix.
= 686.08 Å		1.34 Å/pix.
x 512 pix.
= 686.08 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 1.34 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.058786124 - 2.3520274
Average (Standard dev.)0.0059199724 (±0.05609674)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 686.08 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_72715_msk_1.map
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Half map: #2

Fileemd_72715_half_map_1.map
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Half map: #1

Fileemd_72715_half_map_2.map
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Sample components

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Entire : conoid fiber from Toxoplasma gondii (24-nm repeat)

EntireName: conoid fiber from Toxoplasma gondii (24-nm repeat)
Components
  • Complex: conoid fiber from Toxoplasma gondii (24-nm repeat)
    • Protein or peptide: CF2, TGME49_258090
    • Protein or peptide: CF1, TGME49_222350
    • Protein or peptide: Tubulin alpha chain
    • Protein or peptide: Tubulin beta chain
    • Protein or peptide: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
    • Protein or peptide: CIP3, TGME49_225020
    • Protein or peptide: CF6, TGME49_245640
    • Protein or peptide: CF4, TGME49_246720
    • Protein or peptide: CF3, TGME49_255895
    • Protein or peptide: CPH1, TGME49_266630
    • Protein or peptide: Microtubule associated protein SPM1
    • Protein or peptide: PRP, TGME49_291880
    • Protein or peptide: CF5, TGME49_297180
    • Protein or peptide: DCX, TGME49_256030
  • Ligand: GUANOSINE-5'-TRIPHOSPHATE
  • Ligand: MAGNESIUM ION
  • Ligand: GUANOSINE-5'-DIPHOSPHATE

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Supramolecule #1: conoid fiber from Toxoplasma gondii (24-nm repeat)

SupramoleculeName: conoid fiber from Toxoplasma gondii (24-nm repeat) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#14
Source (natural)Organism: Toxoplasma gondii (eukaryote)

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Macromolecule #1: CF2, TGME49_258090

MacromoleculeName: CF2, TGME49_258090 / type: protein_or_peptide / ID: 1 / Number of copies: 54 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 106.251133 KDa
SequenceString: MATKQMLPGE GADRSSRPGT RSVARSRGTS PSSAPRLGIS QELTALGSQL QALDRRQQLL FDPSVFPEDS RLAVSFPPFT RKVEDAAEA PHGAVKLRNP HRDGLDRVLG DLSKPVDLGV SLPAELQRDS FAQNDPPGRL PPALKHTSLR KLDEDAVSKF H VGVRQSAR ...String:
MATKQMLPGE GADRSSRPGT RSVARSRGTS PSSAPRLGIS QELTALGSQL QALDRRQQLL FDPSVFPEDS RLAVSFPPFT RKVEDAAEA PHGAVKLRNP HRDGLDRVLG DLSKPVDLGV SLPAELQRDS FAQNDPPGRL PPALKHTSLR KLDEDAVSKF H VGVRQSAR PVSYADALER TRREEDRQEK GAVRFAAATA AVAAAASAVG ADADEIDCVV TVEEQAPPVC VSQGETNGGK SS GPGPGEL RAEQAAAGST VKMPADVYSA PGGAWRAAAR APSPEALAEP KVYSHSQWVD ERRGEGGAPP GQFPFPRGLT EMQ ERMEEE WIDRERRLRA DHKREMERAV AHASEKLSRE YSRRLVFELQ EQEKALLAQM HERHRQALAE IRCISESKTD AEEE TQRFQ REASAKEHQL QKVLHETRLI ESEREALAAK VQHLEAENAS LHASLTPLEK QACSQRAKEE DLQLRLERLK ASNDR LQIQ LQHEQQLAAN FAQKRRGLER EVEVLDEKRA VAEREWKRVA AELRELQERQ AGLCASNAHL QNELDNAIRH GRNLEQ RID EDRSKDDERQ KLSQRLEKLQ EEKETTERRQ ADEIASLRNR IKHLDAVTFQ LRTMRQDFES QQLEVKRLRD ENATLLA EM RHQNKGDHAM KLDQQALQND LITVKQENAD LRKEMNRLIK ERNFAASGGS LPPYTPPRPN SFSTTPSPDN PLAMQLYS S EHSRGCGNAC SGGGRQADPM MTEHDASVCP SLTGPLNRRC FTSDGRPRCV EDLSIPSPLS REACASPALR AQQLAVRCS TSINPVGRST RPPPSVGAAS NPLQHVHCPS AASRLGTEGD HPSYMYDGYR GDSRKATALG ALAEDIPARG LAPAISLPVT AETPEQVAS LESQLLLLAT ERRRIESELS KIPSARGRTA RERQQMQHLE SRLVEVDGTT HRIKQILFQA QRRKN

UniProtKB: Uncharacterized protein

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Macromolecule #2: CF1, TGME49_222350

MacromoleculeName: CF1, TGME49_222350 / type: protein_or_peptide / ID: 2 / Number of copies: 14 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 47.976344 KDa
SequenceString: MQPLAREVSR LPRSGPLGPV AAKKDDKTFI PTNVRVSPGE KNDRVVKTEK IDDSTDGSGS HDGPRNLETQ LEALTREVRE LTLRVQEEK EMKELLNSQL NDAYEELADA RSEHRVRGFA GASGARLRGV RGLPAFEARW MEPEHPTAQT AIRSQQEFEL L LQEERARR ...String:
MQPLAREVSR LPRSGPLGPV AAKKDDKTFI PTNVRVSPGE KNDRVVKTEK IDDSTDGSGS HDGPRNLETQ LEALTREVRE LTLRVQEEK EMKELLNSQL NDAYEELADA RSEHRVRGFA GASGARLRGV RGLPAFEARW MEPEHPTAQT AIRSQQEFEL L LQEERARR HTMFEQYERH MKVMRKNHHK EKLETKKKVL SQVEFLIKKY RRLASDAVAV AKRERERVAE ERKRARELAE RA CKNFEAD LKGRTKTALE QYRRLVQEAH TAAKQEREEL HNKCKELQET VDNERREFDT LIVREAEAKM AKFRASYARM KQQ TEEERR QYGVMMHEIA KRVEKECAEY EEFVIRTMRD LLQSKGVEMT ESGLRDFFQK KREEMRPSGE GHSSTDGLVT VKYR FQAAD PEDLQ

UniProtKB: Uncharacterized protein

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Macromolecule #3: Tubulin alpha chain

MacromoleculeName: Tubulin alpha chain / type: protein_or_peptide / ID: 3 / Number of copies: 63 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 50.166645 KDa
SequenceString: MREVISIHVG QAGIQIGNAC WELFCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCVFLDL EPTVVDEVRT GTYRHLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLSL DRIRKLADNC TGLQGFLMFN AVGGGTGSGL GCLLLERLSV D YGKKSKLN ...String:
MREVISIHVG QAGIQIGNAC WELFCLEHGI QPDGQMPSDK TIGGGDDAFN TFFSETGAGK HVPRCVFLDL EPTVVDEVRT GTYRHLFHP EQLISGKEDA ANNFARGHYT IGKEIVDLSL DRIRKLADNC TGLQGFLMFN AVGGGTGSGL GCLLLERLSV D YGKKSKLN FCSWPSPQVS TAVVEPYNSV LSTHSLLEHT DVAVMLDNEA IYDICRRNLD IERPTYTNLN RLIAQVISSL TA SLRFDGA LNVDVTEFQT NLVPYPRIHF MLSSYAPIIS AEKAYHEQLS VAEITNSAFE PASMMAKCDP RHGKYMACCL MYR GDVVPK DVNAAVATIK TKRTIQFVDW CPTGFKCGIN YQPPTVVPGG DLAKVMRAVC MISNSTAIAE VFSRMDHKFD LMYA KRAFV HWYVGEGMEE GEFSEAREDL AALEKDYEEV GIETAEGEGE EEGYGDEY

UniProtKB: Tubulin alpha chain

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Macromolecule #4: Tubulin beta chain

MacromoleculeName: Tubulin beta chain / type: protein_or_peptide / ID: 4 / Number of copies: 57 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 50.119121 KDa
SequenceString: MREIVHVQGG QCGNQIGAKF WEVISDEHGI DPTGTYCGDS DLQLERINVF YNEATGGRFV PRAILMDLEP GTMDSVRAGP FGQLFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAEGCDC LQGFQITHSL GGGTGSGMGT LLISKVREEY P DRIMETFS ...String:
MREIVHVQGG QCGNQIGAKF WEVISDEHGI DPTGTYCGDS DLQLERINVF YNEATGGRFV PRAILMDLEP GTMDSVRAGP FGQLFRPDN FVFGQTGAGN NWAKGHYTEG AELIDSVLDV VRKEAEGCDC LQGFQITHSL GGGTGSGMGT LLISKVREEY P DRIMETFS VFPSPKVSDT VVEPYNATLS VHQLVENADE VQVIDNEALY DICFRTLKLT TPTYGDLNHL VSAAMSGVTC CL RFPGQLN SDLRKLAVNL IPFPRLHFFL IGFAPLTSRG SQQYRALSVP ELTQQMFDAK NMMCASDPRH GRYLTASAMF RGR MSTKEV DEQMLNVQNK NSSYFVEWIP NNMKSSVCDI PPKGLKMSVT FVGNSTAIQE MFKRVSDQFT AMFRRKAFLH WYTG EGMDE MEFTEAESNM NDLVSEYQQY QDATAEEEGE FDEEEGEMGA EEGA

UniProtKB: Tubulin beta chain

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Macromolecule #5: Spindle assembly abnormal protein 6 N-terminal domain-containing ...

MacromoleculeName: Spindle assembly abnormal protein 6 N-terminal domain-containing protein
type: protein_or_peptide / ID: 5 / Number of copies: 41 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 29.529971 KDa
SequenceString: MATNFGFGGG ASGCTDSVNF LAQVDTSAID EMDPSLADGH TLVYSREVPF EIRLQETGSS LPGDVGRLEP IRVKILILGE ETNPQHVRV ELTSENDLFL NYTHALNEDL FRQMQERQKL MIEFGDYPSV LIKMLNSCIK EPHCFLAVLI MHTNGKARLD F IQSMEYKF ...String:
MATNFGFGGG ASGCTDSVNF LAQVDTSAID EMDPSLADGH TLVYSREVPF EIRLQETGSS LPGDVGRLEP IRVKILILGE ETNPQHVRV ELTSENDLFL NYTHALNEDL FRQMQERQKL MIEFGDYPSV LIKMLNSCIK EPHCFLAVLI MHTNGKARLD F IQSMEYKF IELLCLDCVQ STEEFTRRDM TFRYNALKSK LALLQARLHD IGCLLKLKSP SLLLHLQKAA QQQAHRRQQQ VA AAAPIPG KLRAPGTTEG ASTRFL

UniProtKB: Spindle assembly abnormal protein 6 N-terminal domain-containing protein

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Macromolecule #6: CIP3, TGME49_225020

MacromoleculeName: CIP3, TGME49_225020 / type: protein_or_peptide / ID: 6 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 114.592602 KDa
SequenceString: MATQLGRSAP LSECEFLVAS GRARAPGDPS RRSVSQPSRR NGSFREGLGP RSHDRELVGH PDIDKFRSTA GPQTSRARSA RAAQPETLP RFYRPSSRMA AAKNETSREL RGKPPRPTST LKYVGCPPTE PRLRPKRTST GLEERMLKSG GYGVRIHWDE Y AGYHVHAS ...String:
MATQLGRSAP LSECEFLVAS GRARAPGDPS RRSVSQPSRR NGSFREGLGP RSHDRELVGH PDIDKFRSTA GPQTSRARSA RAAQPETLP RFYRPSSRMA AAKNETSREL RGKPPRPTST LKYVGCPPTE PRLRPKRTST GLEERMLKSG GYGVRIHWDE Y AGYHVHAS APEVDLMHAD QVLMVRDSPT HSRESDKDAA GSPPGGLSAS LSPGRKATKK AGPVHCGVSR QCIPVYQFAP TL CEKSREI IDVYRKERVA HLWKKVDARR EGVVDLFQLA SRAPSRKTSK LRSSGMDAKS PDDTVLSSVE ERFVQACVMQ FVQ IAVSRT SAQLVKDGAT LGPHFDDSLR FTTAGAQGSE NPPVVVPTEQ AVFPHPHARD PMREGLQPDA EAEGAKIERE NATA IQAAK RLARQHNQVI MLNVDANLGA DASATPALFV PRKKFESVAL EALAHEFGNP DIDVFRDRVM CECRKLVEKV NPEEA QRFF KPYILEYSRK LDARRRRAKA QEREESGRRA ASLSADGYGP QGKFPTVGLR RVENRFDEQI EHYLRKKQQL HHLKEA LDE MREGQFCTFH PAVNPYPKYL KQAFRLRRSP DDPLVILERF CEQYTEDREY PRLVEAIREC TFQPNIHKFV AKEKQLQ AT GVEVSQEKEK GITVPGKRIK EAHKQTTEDK PAAMESRVDH LFQSIERPFD HIFAADINQW LETDANLYSS QEDGAEKR K KQRWKRWEVE GRAPQYRGAL KDRSQRALLT QDGGEVVVQL VEETEERAPV VLPRHRTTPY NDWVNGLRGG YLPNVMDFQ KGPTEKGDRK KAERFNMRDW SRHIRLSERE VETRIIPNEE IIDAVCESEL PSAAPPPPTI WVRRRRWEPA PERGPDAPTF TEAHFHTGS DRQTDRLLPA GKPAALTQQQ AKEYKNRFAS SVDPTTFVVP TPLKLDRLRQ QYRLYMQLRN GIETGEIRTP P KVVTLRRD VLTDLEKEVK REPPTLVLAE TRDAFASDTV ETDNSVDGYV CE

UniProtKB: Uncharacterized protein

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Macromolecule #7: CF6, TGME49_245640

MacromoleculeName: CF6, TGME49_245640 / type: protein_or_peptide / ID: 7 / Number of copies: 24 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 46.600648 KDa
SequenceString: MNQSTIVATS VVAPQSAVSL SRAPSRPGPS ESFGKQQESR PGVSGAGLAE SKRVPSLTQP SLERSVTISR RKIDAVGMSL VPKLDRTTT SLAAKEEKFS SIDKIVSKPT HSFGESSKLP AGIMKTKSMF PSQTLSAPWN APARCARKDD FGTKAWIEKL Q RETTDTSQ ...String:
MNQSTIVATS VVAPQSAVSL SRAPSRPGPS ESFGKQQESR PGVSGAGLAE SKRVPSLTQP SLERSVTISR RKIDAVGMSL VPKLDRTTT SLAAKEEKFS SIDKIVSKPT HSFGESSKLP AGIMKTKSMF PSQTLSAPWN APARCARKDD FGTKAWIEKL Q RETTDTSQ PPLERQKSQR LAQTEPVQKL KTSWLEPPQE VESGHGVAEG DDLSVAAAEY HVPETEDGKL SFKPSDPRVW NR EWIHRRI HNPVLSRSNR ERCAFTQSND WLVFALHHQY VRPLRSTDWL PGEACISQRD SFQLPPGAAK YTSSSLAIPQ TSW EPFIFP NPPRPFLLRN KTFNSSARTW IPSRKVKAGL VGGGTEHACN LTQHEWLDLE RMLWFDSEPR LEQPYRLYAD SKTC IKGSV LDIGHMMRIG W

UniProtKB: Uncharacterized protein

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Macromolecule #8: CF4, TGME49_246720

MacromoleculeName: CF4, TGME49_246720 / type: protein_or_peptide / ID: 8 / Number of copies: 9 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 56.022809 KDa
SequenceString: MVARVVEQDV ARGSGRDTLA RESDEAHPPQ FSLSNPHFLT AVCEELKRRD KPVTGLTTAE RFNAALADMG VRYGTPEADM IMRYCQVTE DGYVIFKELM LATRQLSNVS EDHVTESLSS ATQREQDRLV PLPNTSARDR DSEQGEIRDE RCVYTPELTD A IRRLYAQW ...String:
MVARVVEQDV ARGSGRDTLA RESDEAHPPQ FSLSNPHFLT AVCEELKRRD KPVTGLTTAE RFNAALADMG VRYGTPEADM IMRYCQVTE DGYVIFKELM LATRQLSNVS EDHVTESLSS ATQREQDRLV PLPNTSARDR DSEQGEIRDE RCVYTPELTD A IRRLYAQW DRSCLRDWQF KESLQRLGVC VTPEFERLLS TYGPSGCVSF SQVMQTLMMS DSGFLASSSL RRSRNRSAAD IP LPPVADR LPFYEPRRNP VTWSPPQPLK GLPVNPQDVL QHALKLPLAA DLDLDRRVGS EVGDTEGGDG CSLADKFRLL KKL VALYLS ERLSATQFRK ELVEADVPIT PELDTLIRAH EADNSGQFTP FAVAVFRAAE ETEIFLKEVR RASANTEAEA HSTR DTPYI VVDKLTQVDV PSTFPAQVEE EEDLKASARH MLEAGVKYPT EKEAFVGESK LGDIPKGNYI PSDYGMSQHA TLALA NRAA AHHAYHGHGN IITWGKSC

UniProtKB: EF hand protein

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Macromolecule #9: CF3, TGME49_255895

MacromoleculeName: CF3, TGME49_255895 / type: protein_or_peptide / ID: 9 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 62.988641 KDa
SequenceString: MTTASAIIGP SGGRDALMAR QLYPNFFKTR AGDPPERGAT RKDLRASMMD RKSILYPIPY LESEASRATW TQRATRGVPH LSTFSLAHA EPGGDIERDE IRETVASLLE VQPSGAQPLS VVYKRHDLEP LPPSISFRPH HNLADPVYYD MPPSPPYPAP P LLLKEQRR ...String:
MTTASAIIGP SGGRDALMAR QLYPNFFKTR AGDPPERGAT RKDLRASMMD RKSILYPIPY LESEASRATW TQRATRGVPH LSTFSLAHA EPGGDIERDE IRETVASLLE VQPSGAQPLS VVYKRHDLEP LPPSISFRPH HNLADPVYYD MPPSPPYPAP P LLLKEQRR QKSLWSPLCN AESPEKRPHS IVFNPGCSFL SVTRGCRRGR RSNSAASEVS FQEVTGEPYH DLVQAESLRS RH RGREAAQ LLSSRLGSLD EPQTNRHFPS PERRRKRAGA TLGILPSRSV SVSAGSHRGR CRSSYPEESV PIPMPFWGRY DWY PPMKAP RQRIQAFGRP ASRECVPLYE SGLDDWTTFP WNETLRTTGH RSLTSEDRSP STRFSPHRGS LRATGDNADL EWRM DRSTN FGELQSRIAD ASRGQQKFYR RQKASLWGAL HPATQRVVGT FPRTECSFVC KEHRGRHVHL MDVFGPVERN RLSSY FDQD EADEIHRRAR GCLGVHTASC PQSHNTLRKV LSDCAERGKP SDLDAFPQQT REPKRHWWSP AEPLADYADE KS

UniProtKB: Bax inhibitor 1, related protein

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Macromolecule #10: CPH1, TGME49_266630

MacromoleculeName: CPH1, TGME49_266630 / type: protein_or_peptide / ID: 10 / Number of copies: 28 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 67.410492 KDa
SequenceString: MSRAYADLVV SRKAKAPPFS VDNNDGRPKR PNRVWFDRDR AKACNDMRRE YPLHYYISSY DVYAFLATLR QGADPAKRDA AGRTPLDLA VQMAVELIET SLCTSFPIDN IDVTPAATLK PAKDSPPESR PETFTNPFRV KYRDANASMY VQHDIMRYRC A DSDVYKSC ...String:
MSRAYADLVV SRKAKAPPFS VDNNDGRPKR PNRVWFDRDR AKACNDMRRE YPLHYYISSY DVYAFLATLR QGADPAKRDA AGRTPLDLA VQMAVELIET SLCTSFPIDN IDVTPAATLK PAKDSPPESR PETFTNPFRV KYRDANASMY VQHDIMRYRC A DSDVYKSC REPPFPLPSD GHDGAERTYQ VATFPPQEFR IKNGDSEDFF RDQQRARRTQ SPSFPLPRGD VSRYYAAAHN LR PQQFAYA YDANGKPMSW SRLQREQVQL EQEHLQGEFL VAVEQFLERP PSKAAMSQMR ELIRRLNLMM VIIKKYELCL PLK REAAEK AKAYIGTALT YPYLYTASMY EAFKRYPRTP AGQAWDALNP ADSRRLVLII LSLKMHGHEL FSFMGTVCRL FNSL MEQLG LCDDRCGTGG HWCKLGSECS QFYLHMAFSL DDAQLFQKFI TQQQLFDKQD IFKWEDLLYT LMQHRERFRP YFAAL LARR LKARVQKRLM NSADQQAYLP ETVKYPCEER YFTRQLLGEY SEIFGESEAE EFNSMLRHWR YGAARQAPGT GSEEDN ILP APLPPVSESP AADFWSLRD

UniProtKB: Ankyrin repeat-containing protein

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Macromolecule #11: Microtubule associated protein SPM1

MacromoleculeName: Microtubule associated protein SPM1 / type: protein_or_peptide / ID: 11 / Number of copies: 5 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 38.84575 KDa
SequenceString: MSGGNSNTPK KLPSEEGSDY GYPQKPQKYL PKSEQAEPDY SACCKGNDAY KGASHGTVQF SHPEEAQKYA GAAAGAETIQ RGRERVAAD RQPRAAGDVP ARRLHLSDVD EAHRGQSPSR HPGYCVEELC TCGMHKCIPS RAPVPFTGST QYRQEFVPKP L PPPTQVSQ ...String:
MSGGNSNTPK KLPSEEGSDY GYPQKPQKYL PKSEQAEPDY SACCKGNDAY KGASHGTVQF SHPEEAQKYA GAAAGAETIQ RGRERVAAD RQPRAAGDVP ARRLHLSDVD EAHRGQSPSR HPGYCVEELC TCGMHKCIPS RAPVPFTGST QYRQEFVPKP L PPPTQVSQ VTLPPSLPFE AESSYRTEFV AKPLPPPAKF SEVKLPPTLP FHGESAYRTD YVPKPLPEVA KPVEVKLPPT LP FNAQSCY RSEYVAKPLP PPVQTVEVKL PPSLPFEGST HYRDEFQVKP LPPATKVTEV KLPPSLPFDA TSMYRSDYVA KSN PICPVS KLPQYPAATY PQNHVFWDPD TKQWY

UniProtKB: Microtubule associated protein SPM1

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Macromolecule #12: PRP, TGME49_291880

MacromoleculeName: PRP, TGME49_291880 / type: protein_or_peptide / ID: 12 / Number of copies: 35 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 80.452414 KDa
SequenceString: MLARGDDELC SSFYRSANKH IRGVGGEPPA SSRADLRRYM PGYTAPPSLN AYKAWHVNSA TLRRENDRLK AENKNIERDF DRLYQRAYI AEVQLNNLKA AASKIPLGGA NYSVTQLVKL AANLLELLTK QPEQTCVSVI PDALACLSMC HVRDRRLFAA G EKLKRTLQ ...String:
MLARGDDELC SSFYRSANKH IRGVGGEPPA SSRADLRRYM PGYTAPPSLN AYKAWHVNSA TLRRENDRLK AENKNIERDF DRLYQRAYI AEVQLNNLKA AASKIPLGGA NYSVTQLVKL AANLLELLTK QPEQTCVSVI PDALACLSMC HVRDRRLFAA G EKLKRTLQ CPSGASGYNG NESKTRPIGR PAGEVAPDLK WLATVSDDLS WLKWADQWDV LPMDLRWISI AVDDRSLLLH RF LWTRAPV DLRYLTVVSG NMDWLYIGPD RWYSTPMQLR LRAIALEDPT IIKPTMAEAA LYGLEREKIA NTLIPDADLP NLP SPHAPE EDLDLTLRIS LSPADIAVCE NNGGIASSAK AGASTGTPGM PTPGSKVGQA KETSASSKVG MAKTVPSPLS KTAP PLKAD PSEPVPTKVG GGSVGGPKAT PSPSKAGPPP KDSDKPGGVA KKASPTPPSK AEGVAASGSG DAPEEPKSVK GAPLG KKGP GPLGVKGGSP LPPPGDAKGK ASPESATAKG PAELPKGPAG AAKAAVPGKA APVGPGKGPA APLGKKAPGE PVGKPK AEM KKSSPPVGEP KAEVKKSPPP PSGSPKAESK KSPSPPLGEP KADVKKSPPP PLGGPKAEMK KSPPGASPAA PKADSST PA APTGATPPKA KLGAPPGKAP TAGTAEGKEA APAKKGPLPA KGAEPLGKKG PPPVAKAEDT TKPPPPTAGQ NDDADGAG V SAKKQSPLGK GPPPKKGPLL GKKIPPAPDA RDAKADDASP AKKGPPKGLP KGKAPPKGKG FL

UniProtKB: Uncharacterized protein

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Macromolecule #13: CF5, TGME49_297180

MacromoleculeName: CF5, TGME49_297180 / type: protein_or_peptide / ID: 13 / Number of copies: 7 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 97.829719 KDa
SequenceString: MSRERLGPSR LGRHTPAAAS ARGDPRLLVE AETPTDRGRI VKASLHTQGD VEFLEGRLRD KVCVDKWVTV QERASPAEGK RESEGKRES EGRRESPRMT STSAGLEPAE AWREDIFGKE ERYDMARFDE EDASAEDAVR TAPRPSPQRQ FQVRHKVTVE G VVPTSASV ...String:
MSRERLGPSR LGRHTPAAAS ARGDPRLLVE AETPTDRGRI VKASLHTQGD VEFLEGRLRD KVCVDKWVTV QERASPAEGK RESEGKRES EGRRESPRMT STSAGLEPAE AWREDIFGKE ERYDMARFDE EDASAEDAVR TAPRPSPQRQ FQVRHKVTVE G VVPTSASV RMQRRLAQRR GKRFFSTDEG LYWQKLQRSK PQKSREDALK FVATFMQEGE LLVNSVSMEE DPSVRLRRLA AL RLLRNSP ADRVPGSISG RLLIPYDHAD VRELLRVSSF CSGEAWRVLE EIRRLLVLRK GRLFLASDRN ATASRHCLAF LAS LKKNLE AAARNLVRQG RLRRPSARLQ QSEGRKRAPT LDRDEAWERR EGLVREIRRT LSLAAAYSTK GKKQPVVYIQ LAAL KPSIV ADFLTPAEVA VILTEVFQNL WTKFVEYSLR RRVRVSTAAL VLDFSGLTEF ELASSASHFL LSSLRDVVRA FAPLL IKKI IFYNSAKAGE FLWEALRPGV EHSCFFSFCS SEEDLETEIE SERTMVYHTT GLANDMSLIA HRCPLYALYG PPLPPP DSL FPLRAPGFLP ASAPASALQK SPLSRPKAGA FRQLFALLGA ARVRLVPTAE DAETGGSNLP RAPFFRTAGE SRERENA GL ARGGEEDEET ETLRRGDEEI QKTCREEEAK FWRGMNCFHD LVVEAARAKR IAERERQADV VESARQTRPA EAKPDEAD E DAEELDLDSV DVIDPDANLG PSEESLECAA AAVAQDGDRV DLGKSHAERS FERIFTAQGD LEETLEGIGS PFENAWERS VQEAFDASVK AIREEHLPLS AARVELPRVS RPARTFPTPL DRVFRRPLYL HLSAEAGGNW ERELLGADRA K

UniProtKB: CRAL/TRIO domain-containing protein

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Macromolecule #14: DCX, TGME49_256030

MacromoleculeName: DCX, TGME49_256030 / type: protein_or_peptide / ID: 14 / Number of copies: 27 / Enantiomer: LEVO
Source (natural)Organism: Toxoplasma gondii (eukaryote)
Molecular weightTheoretical: 29.261402 KDa
SequenceString: MACGIPWKLA RRDELMATRQ AERPGEYFPP PYPPCPPTVV TPLRTSAYDF PEATFVTRPC LPAKKATGHK NVFERLTDTA YYTGSHRER FDEFGNGRGI AGREYLYAYD GLTESPSRCH EVYSSVIKRP RKPVVTPGTL GIQRFGVQIP APRLMWLYRN G DKHDDGTP ...String:
MACGIPWKLA RRDELMATRQ AERPGEYFPP PYPPCPPTVV TPLRTSAYDF PEATFVTRPC LPAKKATGHK NVFERLTDTA YYTGSHRER FDEFGNGRGI AGREYLYAYD GLTESPSRCH EVYSSVIKRP RKPVVTPGTL GIQRFGVQIP APRLMWLYRN G DKHDDGTP FFVRPYIKSM ESLYQQITKE ITPIAGPVRR IFDQNFRVIT DLDDIVDGAK YLCTSGEPPA AYDRLEKFLS EW VIQKSQT KVPSQFFVV

UniProtKB: Doublecortin domain-containing protein

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Macromolecule #15: GUANOSINE-5'-TRIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 15 / Number of copies: 63 / Formula: GTP
Molecular weightTheoretical: 523.18 Da
Chemical component information

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

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Macromolecule #16: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 16 / Number of copies: 54 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #17: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 17 / Number of copies: 57 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation statefilament

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 25.0 µm / Nominal defocus min: 5.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.14 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 95409
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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