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Structure paper

TitleStructural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 141, Year 2025
Publish dateJan 2, 2025
AuthorsSyed Zawar Shah / Thomas N Perry / Andrea Graziadei / Valentina Cecatiello / Thangavelu Kaliyappan / Agata D Misiaszek / Christoph W Müller / Ewan P Ramsay / Alessandro Vannini /
PubMed AbstractRNA polymerase III (Pol III) transcribes short, essential RNAs, including the U6 small nuclear RNA (snRNA). At U6 snRNA genes, Pol III is recruited by the snRNA Activating Protein Complex (SNAPc) and ...RNA polymerase III (Pol III) transcribes short, essential RNAs, including the U6 small nuclear RNA (snRNA). At U6 snRNA genes, Pol III is recruited by the snRNA Activating Protein Complex (SNAPc) and a Brf2-containing TFIIIB complex, forming a pre-initiation complex (PIC). Uniquely, SNAPc also recruits Pol II at the remaining splicesosomal snRNA genes (U1, 2, 4 and 5). The mechanism of SNAPc cross-polymerase engagement and the role of the SNAPC2 and SNAPC5 subunits remain poorly defined. Here, we present cryo-EM structures of the full-length SNAPc-containing Pol III PIC assembled on the U6 snRNA promoter in the open and melting states at 3.2-4.2 Å resolution. The structural comparison revealed differences with the Saccharomyces cerevisiae Pol III PIC and the basis of selective SNAPc engagement within Pol III and Pol II PICs. Additionally, crosslinking mass spectrometry localizes SNAPC2 and SNAPC5 near the promoter DNA, expanding upon existing descriptions of snRNA Pol III PIC structure.
External linksNat Commun / PubMed:39747245 / PubMed Central
MethodsEM (single particle)
Resolution3.28 - 4.14 Å
Structure data

50730

9fso

EMDB-50730, PDB-9fso:
Human RNA Polymerase III Class III Open Pre-initiation Complex 1 (OC1)
Method: EM (single particle) / Resolution: 3.28 Å

50731

9fsp

EMDB-50731, PDB-9fsp:
RNA Polymerase III Class III Open Pre-initation Complex 2 (OC2)
Method: EM (single particle) / Resolution: 3.39 Å

50732

9fsq

EMDB-50732, PDB-9fsq:
RNA Polymerase III Class III Melting Pre-Initiation Complex (MC)
Method: EM (single particle) / Resolution: 3.51 Å

50733

9fsr

EMDB-50733, PDB-9fsr:
RNA Polymerase III Class III Open Mini Pre-initiation Complex 1 (OC1-mini)
Method: EM (single particle) / Resolution: 3.76 Å

50734

9fss

EMDB-50734, PDB-9fss:
RNA Polymerase III Class III Open Mini Pre-Initiation complex 2 (OC2-mini)
Method: EM (single particle) / Resolution: 4.14 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-SF4:
IRON/SULFUR CLUSTER

Source
  • homo sapiens (human)
  • homo (humans)
KeywordsTRANSCRIPTION / RNA Polymerase / SNAPc / TFIIIB / snRNA / pre-initiation complex / U6_2 / RNA Polymerase III

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