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- EMDB-50734: RNA Polymerase III Class III Open Mini Pre-Initiation complex 2 (... -

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Basic information

Entry
Database: EMDB / ID: EMD-50734
TitleRNA Polymerase III Class III Open Mini Pre-Initiation complex 2 (OC2-mini)
Map data
Sample
  • Complex: RNA Polymerase III Class III Open Mini Pre-Initiation complex 2
    • Complex: SNAPc
      • Protein or peptide: x 3 types
    • Complex: TFIIIB
      • Protein or peptide: x 3 types
    • Complex: DNA
      • DNA: x 2 types
    • Complex: RNA Polymerase III
      • Protein or peptide: x 17 types
  • Ligand: x 3 types
KeywordsRNA Polymerase III / SNAPc / TFIIIB / snRNA / Transcription
Function / homology
Function and homology information


snRNA-activating protein complex / snRNA transcription / transcription preinitiation complex assembly / snRNA transcription by RNA polymerase III / bent DNA binding / RNA polymerase III preinitiation complex assembly / RNA Polymerase III Chain Elongation / transcription factor TFIIIB complex / DNA/RNA hybrid binding / calcitonin gene-related peptide receptor activity ...snRNA-activating protein complex / snRNA transcription / transcription preinitiation complex assembly / snRNA transcription by RNA polymerase III / bent DNA binding / RNA polymerase III preinitiation complex assembly / RNA Polymerase III Chain Elongation / transcription factor TFIIIB complex / DNA/RNA hybrid binding / calcitonin gene-related peptide receptor activity / TFIIIC-class transcription factor complex binding / RNA Polymerase III Transcription Termination / RNA polymerase transcription factor SL1 complex / RNA polymerase III type 3 promoter sequence-specific DNA binding / regulation of transcription by RNA polymerase III / snRNA transcription by RNA polymerase II / RPAP3/R2TP/prefoldin-like complex / regulation of transcription by RNA polymerase I / RNA polymerase III general transcription initiation factor activity / RNA polymerase I core promoter sequence-specific DNA binding / DNA polymerase III complex / RNA Polymerase III Transcription Initiation From Type 1 Promoter / RNA Polymerase III Transcription Initiation From Type 2 Promoter / RNA Polymerase III Transcription Initiation From Type 3 Promoter / transcription factor TFIIA complex / RNA Polymerase III Abortive And Retractive Initiation / Cytosolic sensors of pathogen-associated DNA / male pronucleus / female pronucleus / positive regulation of innate immune response / RNA polymerase II general transcription initiation factor binding / nucleobase-containing compound metabolic process / Abortive elongation of HIV-1 transcript in the absence of Tat / FGFR2 alternative splicing / transcription preinitiation complex / RNA Polymerase I Transcription Termination / Viral Messenger RNA Synthesis / MicroRNA (miRNA) biogenesis / Signaling by FGFR2 IIIa TM / transcription factor TFIID complex / RNA polymerase II general transcription initiation factor activity / RNA Pol II CTD phosphorylation and interaction with CE during HIV infection / RNA Pol II CTD phosphorylation and interaction with CE / Formation of the Early Elongation Complex / Formation of the HIV-1 Early Elongation Complex / mRNA Capping / DNA-templated transcription elongation / HIV Transcription Initiation / RNA Polymerase II HIV Promoter Escape / Transcription of the HIV genome / RNA Polymerase II Promoter Escape / RNA Polymerase II Transcription Pre-Initiation And Promoter Opening / RNA Polymerase II Transcription Initiation / RNA Polymerase II Transcription Initiation And Promoter Clearance / PIWI-interacting RNA (piRNA) biogenesis / Processing of Capped Intron-Containing Pre-mRNA / mRNA Splicing - Minor Pathway / positive regulation of transcription by RNA polymerase III / aryl hydrocarbon receptor binding / TFIIB-class transcription factor binding / RNA Polymerase I Transcription Initiation / RNA polymerase II transcribes snRNA genes / Pausing and recovery of Tat-mediated HIV elongation / Tat-mediated HIV elongation arrest and recovery / HIV elongation arrest and recovery / Pausing and recovery of HIV elongation / termination of RNA polymerase I transcription / transcription by RNA polymerase III / positive regulation of transcription initiation by RNA polymerase II / nucleolar large rRNA transcription by RNA polymerase I / transcription initiation at RNA polymerase I promoter / Tat-mediated elongation of the HIV-1 transcript / Formation of HIV-1 elongation complex containing HIV-1 Tat / RNA polymerase II core promoter sequence-specific DNA binding / core promoter sequence-specific DNA binding / RNA polymerase II preinitiation complex assembly / Formation of HIV elongation complex in the absence of HIV Tat / termination of RNA polymerase III transcription / transcription initiation at RNA polymerase III promoter / RNA polymerase I complex / RNA Polymerase II Transcription Elongation / RNA polymerase III complex / Formation of RNA Pol II elongation complex / RNA polymerase II, core complex / tRNA transcription by RNA polymerase III / transcription by RNA polymerase I / transcription elongation by RNA polymerase I / RNA Polymerase II Pre-transcription Events / mRNA Splicing - Major Pathway / mRNA Polyadenylation / positive regulation of interferon-beta production / acrosomal vesicle / TBP-class protein binding / Inhibition of DNA recombination at telomere / SIRT1 negatively regulates rRNA expression / TP53 Regulates Transcription of DNA Repair Genes / Transcriptional regulation by small RNAs / RNA Polymerase I Promoter Escape / DNA-templated transcription initiation / euchromatin
Similarity search - Function
snRNA-activating protein complex subunit 1 / snRNA-activating protein complex, subunit 3 / Small nuclear RNA activating complex (SNAPc), subunit 1 / snRNA-activating protein complex (SNAPc), subunit 3 / : / : / BRF2-like, C-terminal cyclin repeat / Transcription factor TFIIIB component B'', Myb domain / Myb DNA-binding like / DNA-directed RNA polymerase III subunit RPC5, C-terminal ...snRNA-activating protein complex subunit 1 / snRNA-activating protein complex, subunit 3 / Small nuclear RNA activating complex (SNAPc), subunit 1 / snRNA-activating protein complex (SNAPc), subunit 3 / : / : / BRF2-like, C-terminal cyclin repeat / Transcription factor TFIIIB component B'', Myb domain / Myb DNA-binding like / DNA-directed RNA polymerase III subunit RPC5, C-terminal / DNA-directed RNA polymerase III subunit RPC5 C-terminal / DNA-directed RNA polymerase III subunit RPC4 / RNA polymerase III RPC4 / DNA-directed RNA polymerase III, subunit Rpc31 / DNA-directed RNA polymerase III subunit Rpc31 / Pol III subunit C11, C-terminal zinc ribbon / DNA-directed RNA polymerase III subunit Rpc5 / : / DNA-directed RNA polymerase III subunit RPC1, N-terminal / DNA-directed RNA polymerase III subunit RPC1, C-terminal / RPC5 protein / RNA polymerase III, subunit Rpc25 / DNA-directed RNA polymerase III subunit RPC9 / RNA polymerase III subunit Rpc25 / RNA polymerase Rpc34 / RNA polymerase III Rpc82, C -terminal / RNA polymerase Rpc34-like / DNA-directed RNA polymerase III subunit RPC3 / : / RNA polymerase Rpc34 subunit / RNA polymerase III subunit RPC82 / DNA-directed RNA polymerase III subunit RPC3, helical hairpin domain / POLR3C, C-terminal winged-helix domain / RNA polymerase III subunit RPC82-related, helix-turn-helix / RNA polymerase III subunit RPC82 helix-turn-helix domain / Myb-like domain profile. / DNA-directed RNA polymerase, subunit E/RPC8 / Myb-like DNA-binding domain / DNA-directed RNA polymerases I and III subunit AC19 / DNA-directed RNA polymerases I and III subunit AC40 / Zinc finger TFIIB-type profile. / Transcription factor TFIIB / Zinc finger, TFIIB-type / TFIIB zinc-binding / TATA-box binding protein, eukaryotic / TATA-box binding protein / TATA-box binding protein, conserved site / Transcription factor TFIID (or TATA-binding protein, TBP) / Transcription factor TFIID repeat signature. / Myb-type HTH DNA-binding domain profile. / SANT domain / Myb domain / TBP domain superfamily / RNA polymerase Rpb4/RPC9, core / DNA-directed RNA-polymerase II subunit / Cyclin-like superfamily / SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains / Rpb4/RPC9 superfamily / RNA polymerase subunit Rpb4/RPC9 / RNA polymerase Rpb4 / SANT/Myb domain / Zinc finger TFIIS-type signature. / RNA polymerase Rpb7-like , N-terminal / RNA polymerase Rpb7-like, N-terminal domain superfamily / RNA polymerase subunit Rpb7-like / SHS2 domain found in N terminus of Rpb7p/Rpc25p/MJ0397 / HRDC-like superfamily / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 5 / RNA polymerase Rpb2, domain 4 / RNA polymerase Rpb2, domain 4 / DNA-directed RNA polymerase, M/15kDa subunit / RNA polymerases M/15 Kd subunit / RNA polymerase subunit 9 / DNA-directed RNA polymerase M, 15kDa subunit, conserved site / RNA polymerases M / 15 Kd subunits signature. / DNA-directed RNA polymerase subunit/transcription factor S / RNA polymerase, Rpb8 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / RNA polymerase Rpb8 / RNA polymerase subunit 8 / RNA polymerase, Rpb5, N-terminal / RNA polymerase Rpb5, N-terminal domain superfamily / RNA polymerase Rpb5, N-terminal domain / DNA-directed RNA polymerase, subunit RPB6 / : / DNA-directed RNA polymerase subunit RPABC5/Rpb10 / RNA polymerases, subunit N, zinc binding site / RNA polymerase subunit RPB10 / RNA polymerases N / 8 kDa subunit / RNA polymerases N / 8 Kd subunits signature. / RNA polymerase, subunit H/Rpb5, conserved site / RNA polymerases H / 23 Kd subunits signature. / DNA directed RNA polymerase, 7 kDa subunit / Zinc finger, TFIIS-type / RNA polymerase archaeal subunit P/eukaryotic subunit RPABC4 / Transcription factor S-II (TFIIS) / Zinc finger TFIIS-type profile. / C2C2 Zinc finger / RNA polymerase subunit CX
Similarity search - Domain/homology
Transcription factor TFIIIB component B'' homolog / DNA-directed RNA polymerase III subunit RPC1 / DNA-directed RNA polymerases I and III subunit RPAC1 / DNA-directed RNA polymerase III subunit RPC7 / DNA-directed RNA polymerase III subunit RPC9 / DNA-directed RNA polymerase III subunit RPC4 / DNA-directed RNA polymerases I and III subunit RPAC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / TATA-box-binding protein / DNA-directed RNA polymerases I, II, and III subunit RPABC3 ...Transcription factor TFIIIB component B'' homolog / DNA-directed RNA polymerase III subunit RPC1 / DNA-directed RNA polymerases I and III subunit RPAC1 / DNA-directed RNA polymerase III subunit RPC7 / DNA-directed RNA polymerase III subunit RPC9 / DNA-directed RNA polymerase III subunit RPC4 / DNA-directed RNA polymerases I and III subunit RPAC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC1 / TATA-box-binding protein / DNA-directed RNA polymerases I, II, and III subunit RPABC3 / DNA-directed RNA polymerases I, II, and III subunit RPABC4 / DNA-directed RNA polymerases I, II, and III subunit RPABC2 / DNA-directed RNA polymerases I, II, and III subunit RPABC5 / snRNA-activating protein complex subunit 1 / snRNA-activating protein complex subunit 4 / snRNA-activating protein complex subunit 3 / DNA-directed RNA polymerase III subunit RPC3 / DNA-directed RNA polymerase III subunit RPC6 / Transcription factor IIIB 50 kDa subunit / DNA-directed RNA polymerase III subunit RPC5 / DNA-directed RNA polymerase III subunit RPC2 / DNA-directed RNA polymerase III subunit RPC10 / DNA-directed RNA polymerase III subunit RPC8
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 4.14 Å
AuthorsShah SZ / Ramsay EP / Cecatiello V / Perry TN / Vannini A
Funding support Italy, 1 items
OrganizationGrant numberCountry
Other government Italy
CitationJournal: Nat Commun / Year: 2025
Title: Structural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters.
Authors: Syed Zawar Shah / Thomas N Perry / Andrea Graziadei / Valentina Cecatiello / Thangavelu Kaliyappan / Agata D Misiaszek / Christoph W Müller / Ewan P Ramsay / Alessandro Vannini /
Abstract: RNA polymerase III (Pol III) transcribes short, essential RNAs, including the U6 small nuclear RNA (snRNA). At U6 snRNA genes, Pol III is recruited by the snRNA Activating Protein Complex (SNAPc) and ...RNA polymerase III (Pol III) transcribes short, essential RNAs, including the U6 small nuclear RNA (snRNA). At U6 snRNA genes, Pol III is recruited by the snRNA Activating Protein Complex (SNAPc) and a Brf2-containing TFIIIB complex, forming a pre-initiation complex (PIC). Uniquely, SNAPc also recruits Pol II at the remaining splicesosomal snRNA genes (U1, 2, 4 and 5). The mechanism of SNAPc cross-polymerase engagement and the role of the SNAPC2 and SNAPC5 subunits remain poorly defined. Here, we present cryo-EM structures of the full-length SNAPc-containing Pol III PIC assembled on the U6 snRNA promoter in the open and melting states at 3.2-4.2 Å resolution. The structural comparison revealed differences with the Saccharomyces cerevisiae Pol III PIC and the basis of selective SNAPc engagement within Pol III and Pol II PICs. Additionally, crosslinking mass spectrometry localizes SNAPC2 and SNAPC5 near the promoter DNA, expanding upon existing descriptions of snRNA Pol III PIC structure.
History
DepositionJun 21, 2024-
Header (metadata) releaseJan 15, 2025-
Map releaseJan 15, 2025-
UpdateJan 15, 2025-
Current statusJan 15, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_50734.png

Downloads & links

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Map

FileDownload / File: emd_50734.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Projections & slices

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Size
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AxesZ (Sec.)Y (Row.)X (Col.)
0.96 Å/pix.
x 420 pix.
= 401.1 Å		0.96 Å/pix.
x 420 pix.
= 401.1 Å		0.96 Å/pix.
x 420 pix.
= 401.1 Å

Surface

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Images are generated by Spider.

Voxel sizeX=Y=Z: 0.955 Å
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Histogram (log scale)
Contour LevelBy AUTHOR: 0.004
Minimum - Maximum-0.03638172 - 0.06303432
Average (Standard dev.)0.00012302057 (±0.0011703098)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 401.1 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: #2

Fileemd_50734_half_map_1.map
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Half map: #1

Fileemd_50734_half_map_2.map
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Sample components

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Entire : RNA Polymerase III Class III Open Mini Pre-Initiation complex 2

EntireName: RNA Polymerase III Class III Open Mini Pre-Initiation complex 2
Components
  • Complex: RNA Polymerase III Class III Open Mini Pre-Initiation complex 2
    • Complex: SNAPc
      • Protein or peptide: snRNA-activating protein complex subunit 1
      • Protein or peptide: snRNA-activating protein complex subunit 4
      • Protein or peptide: snRNA-activating protein complex subunit 3
    • Complex: TFIIIB
      • Protein or peptide: TATA-box-binding protein
      • Protein or peptide: Transcription factor IIIB 50 kDa subunit
      • Protein or peptide: Transcription factor TFIIIB component B'' homolog
    • Complex: DNA
      • DNA: U6_2_Template
      • DNA: U6_2_Non template
    • Complex: RNA Polymerase III
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC1
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC2
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC3
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC4
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC5
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC6
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC7
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC8
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC9
      • Protein or peptide: DNA-directed RNA polymerase III subunit RPC10
      • Protein or peptide: DNA-directed RNA polymerases I and III subunit RPAC1
      • Protein or peptide: DNA-directed RNA polymerases I and III subunit RPAC2
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC1
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC2
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC3
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC4
      • Protein or peptide: DNA-directed RNA polymerases I, II, and III subunit RPABC5
  • Ligand: ZINC ION
  • Ligand: MAGNESIUM ION
  • Ligand: IRON/SULFUR CLUSTER

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Supramolecule #1: RNA Polymerase III Class III Open Mini Pre-Initiation complex 2

SupramoleculeName: RNA Polymerase III Class III Open Mini Pre-Initiation complex 2
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#25

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Supramolecule #2: SNAPc

SupramoleculeName: SNAPc / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #21-#22, #25
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #3: TFIIIB

SupramoleculeName: TFIIIB / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #18-#20
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #4: DNA

SupramoleculeName: DNA / type: complex / ID: 4 / Parent: 1 / Macromolecule list: #23-#24
Source (natural)Organism: Homo sapiens (human)

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Supramolecule #5: RNA Polymerase III

SupramoleculeName: RNA Polymerase III / type: complex / ID: 5 / Parent: 1 / Macromolecule list: #1-#17
Source (natural)Organism: Homo sapiens (human)

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Macromolecule #1: DNA-directed RNA polymerase III subunit RPC1

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 155.860125 KDa
SequenceString: MVKEQFRETD VAKKISHICF GMKSPEEMRQ QAHIQVVSKN LYSQDNQHAP LLYGVLDHRM GTSEKDRPCE TCGKNLADCL GHYGYIDLE LPCFHVGYFR AVIGILQMIC KTCCHIMLSQ EEKKQFLDYL KRPGLTYLQK RGLKKKISDK CRKKNICHHC G AFNGTVKK ...String:
MVKEQFRETD VAKKISHICF GMKSPEEMRQ QAHIQVVSKN LYSQDNQHAP LLYGVLDHRM GTSEKDRPCE TCGKNLADCL GHYGYIDLE LPCFHVGYFR AVIGILQMIC KTCCHIMLSQ EEKKQFLDYL KRPGLTYLQK RGLKKKISDK CRKKNICHHC G AFNGTVKK CGLLKIIHEK YKTNKKVVDP IVSNFLQSFE TAIEHNKEVE PLLGRAQENL NPLVVLNLFK RIPAEDVPLL LM NPEAGKP SDLILTRLLV PPLCIRPSVV SDLKSGTNED DLTMKLTEII FLNDVIKKHR ISGAKTQMIM EDWDFLQLQC ALY INSELS GIPLNMAPKK WTRGFVQRLK GKQGRFRGNL SGKRVDFSGR TVISPDPNLR IDEVAVPVHV AKILTFPEKV NKAN INFLR KLVQNGPEVH PGANFIQQRH TQMKRFLKYG NREKMAQELK YGDIVERHLI DGDVVLFNRQ PSLHKLSIMA HLARV KPHR TFRFNECVCT PYNADFDGDE MNLHLPQTEE AKAEALVLMG TKANLVTPRN GEPLIAAIQD FLTGAYLLTL KDTFFD RAK ACQIIASILV GKDEKIKVRL PPPTILKPVT LWTGKQIFSV ILRPSDDNPV RANLRTKGKQ YCGKGEDLCA NDSYVTI QN SELMSGSMDK GTLGSGSKNN IFYILLRDWG QNEAADAMSR LARLAPVYLS NRGFSIGIGD VTPGQGLLKA KYELLNAG Y KKCDEYIEAL NTGKLQQQPG CTAEETLEAL ILKELSVIRD HAGSACLREL DKSNSPLTMA LCGSKGSFIN ISQMIACVG QQAISGSRVP DGFENRSLPH FEKHSKLPAA KGFVANSFYS GLTPTEFFFH TMAGREGLVD TAVKTAETGY MQRRLVKSLE DLCSQYDLT VRSSTGDIIQ FIYGGDGLDP AAMEGKDEPL EFKRVLDNIK AVFPCPSEPA LSKNELILTT ESIMKKSEFL C CQDSFLQE IKKFIKGVSE KIKKTRDKYG INDNGTTEPR VLYQLDRITP TQVEKFLETC RDKYMRAQME PGSAVGALCA QS IGEPGTQ MTLKTFHFAG VASMNITLGV PRIKEIINAS KAISTPIITA QLDKDDDADY ARLVKGRIEK TLLGEISEYI EEV FLPDDC FILVKLSLER IRLLRLEVNA ETVRYSICTS KLRVKPGDVA VHGEAVVCVT PRENSKSSMY YVLQFLKEDL PKVV VQGIP EVSRAVIHID EQSGKEKYKL LVEGDNLRAV MATHGVKGTR TTSNNTYEVE KTLGIEAART TIINEIQYTM VNHGM SIDR RHVMLLSDLM TYKGEVLGIT RFGLAKMKES VLMLASFEKT ADHLFDAAYF GQKDSVCGVS ECIIMGIPMN IGTGLF KLL HKADRDPNPP KRPLIFDTNE FHIPLVT

UniProtKB: DNA-directed RNA polymerase III subunit RPC1

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Macromolecule #2: DNA-directed RNA polymerase III subunit RPC2

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC2 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: DNA-directed RNA polymerase
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 127.953891 KDa
SequenceString: MDVLAEEFGN LTPEQLAAPI PTVEEKWRLL PAFLKVKGLV KQHIDSFNYF INVEIKKIMK ANEKVTSDAD PMWYLKYLNI YVGLPDVEE SFNVTRPVSP HECRLRDMTY SAPITVDIEY TRGSQRIIRN ALPIGRMPIM LRSSNCVLTG KTPAEFAKLN E CPLDPGGY ...String:
MDVLAEEFGN LTPEQLAAPI PTVEEKWRLL PAFLKVKGLV KQHIDSFNYF INVEIKKIMK ANEKVTSDAD PMWYLKYLNI YVGLPDVEE SFNVTRPVSP HECRLRDMTY SAPITVDIEY TRGSQRIIRN ALPIGRMPIM LRSSNCVLTG KTPAEFAKLN E CPLDPGGY FIVKGVEKVI LIQEQLSKNR IIVEADRKGA VGASVTSSTH EKKSRTNMAV KQGRFYLRHN TLSEDIPIVI IF KAMGVES DQEIVQMIGT EEHVMAAFGP SLEECQKAQI FTQMQALKYI GNKVRRQRMW GGGPKKTKIE EARELLASTI LTH VPVKEF NFRAKCIYTA VMVRRVILAQ GDNKVDDRDY YGNKRLELAG QLLSLLFEDL FKKFNSEMKK IADQVIPKQR AAQF DVVKH MRQDQITNGM VNAISTGNWS LKRFKMDRQG VTQVLSRLSY ISALGMMTRI SSQFEKTRKV SGPRSLQPSQ WGMLC PSDT PEGEACGLVK NLALMTHITT DMEDGPIVKL ASNLGVEDVN LLCGEELSYP NVFLVFLNGN ILGVIRDHKK LVNTFR LMR RAGYINEFVS ISTNLTDRCV YISSDGGRLC RPYIIVKKQK PAVTNKHMEE LAQGYRNFED FLHESLVEYL DVNEEND CN IALYEHTINK DTTHLEIEPF TLLGVCAGLI PYPHHNQSPR NTYQCAMGKQ AMGTIGYNQR NRIDTLMYLL AYPQKPMV K TKTIELIEFE KLPAGQNATV AVMSYSGYDI EDALVLNKAS LDRGFGRCLV YKNAKCTLKR YTNQTFDKVM GPMLDAATR KPIWRHEILD ADGICSPGEK VENKQVLVNK SMPTVTQIPL EGSNVPQQPQ YKDVPITYKG ATDSYIEKVM ISSNAEDAFL IKMLLRQTR RPEIGDKFSS RHGQKGVCGL IVPQEDMPFC DSGICPDIIM NPHGFPSRMT VGKLIELLAG KAGVLDGRFH Y GTAFGGSK VKDVCEDLVR HGYNYLGKDY VTSGITGEPL EAYIYFGPVY YQKLKHMVLD KMHARARGPR AVLTRQPTEG RS RDGGLRL GEMERDCLIG YGASMLLLER LMISSDAFEV DVCGQCGLLG YSGWCHYCKS SCHVSSLRIP YACKLLFQEL QSM NIIPRL KLSKYNE

UniProtKB: DNA-directed RNA polymerase III subunit RPC2

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Macromolecule #3: DNA-directed RNA polymerase III subunit RPC3

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC3 / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 60.692555 KDa
SequenceString: MTQAEIKLCS LLLQEHFGEI VEKIGVHLIR TGSQPLRVIA HDTGTSLDQV KKALCVLVQH NLVSYQVHKR GVVEYEAQCS RVLRMLRYP RYIYTTKTLY SDTGELIVEE LLLNGKLTMS AVVKKVADRL TETMEDGKTM DYAEVSNTFV RLADTHFVQR C PSVPTTEN ...String:
MTQAEIKLCS LLLQEHFGEI VEKIGVHLIR TGSQPLRVIA HDTGTSLDQV KKALCVLVQH NLVSYQVHKR GVVEYEAQCS RVLRMLRYP RYIYTTKTLY SDTGELIVEE LLLNGKLTMS AVVKKVADRL TETMEDGKTM DYAEVSNTFV RLADTHFVQR C PSVPTTEN SDPGPPPPAP TLVINEKDMY LVPKLSLIGK GKRRRSSDED AAGEPKAKRP KYTTDNKEPI PDDGIYWQAN LD RFHQHFR DQAIVSAVAN RMDQTSSEIV RTMLRMSEIT TSSSAPFTQP LSSNEIFRSL PVGYNISKQV LDQYLTLLAD DPL EFVGKS GDSGGGMYVI NLHKALASLA TATLESVVQE RFGSRCARIF RLVLQKKHIE QKQVEDFAMI PAKEAKDMLY KMLS ENFMS LQEIPKTPDH APSRTFYLYT VNILSAARML LHRCYKSIAN LIERRQFETK ENKRLLEKSQ RVEAIIASMQ ATGAE EAQL QEIEEMITAP ERQQLETLKR NVNKLDASEI QVDETIFLLE SYIECTMKRQ

UniProtKB: DNA-directed RNA polymerase III subunit RPC3

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Macromolecule #4: DNA-directed RNA polymerase III subunit RPC4

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC4 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 44.471875 KDa
SequenceString: MSEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL GGVKKKTFTP NIISRKIKEE PKEEVTVKKE KRERDRDRQ REGHGRGRGR PEVIQSHSIF EQGPAEMMKK KGNWDKTVDV SDMGPSHIIN IKKEKRETDE ETKQILRMLE K DDFLDDPG ...String:
MSEGNAAGEP STPGGPRPLL TGARGLIGRR PAPPLTPGRL PSIRSRDLTL GGVKKKTFTP NIISRKIKEE PKEEVTVKKE KRERDRDRQ REGHGRGRGR PEVIQSHSIF EQGPAEMMKK KGNWDKTVDV SDMGPSHIIN IKKEKRETDE ETKQILRMLE K DDFLDDPG LRNDTRNMPV QLPLAHSGWL FKEENDEPDV KPWLAGPKEE DMEVDIPAVK VKEEPRDEEE EAKMKAPPKA AR KTPGLPK DVSVAELLRE LSLTKEEELL FLQLPDTLPG QPPTQDIKPI KTEVQGEDGQ VVLIKQEKDR EAKLAENACT LAD LTEGQV GKLLIRKSGR VQLLLGKVTL DVTMGTACSF LQELVSVGLG DSRTGEMTVL GHVKHKLVCS PDFESLLDHK HR

UniProtKB: DNA-directed RNA polymerase III subunit RPC4

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Macromolecule #5: DNA-directed RNA polymerase III subunit RPC5

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC5 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 80.004031 KDa
SequenceString: MANEEDDPVV QEIDVYLAKS LAEKLYLFQY PVRPASMTYD DIPHLSAKIK PKQQKVELEM AIDTLNPNYC RSKGEQIALN VDGACADET STYSSKLMDK QTFCSSQTTS NTSRYAAALY RQGELHLTPL HGILQLRPSF SYLDKADAKH REREAANEAG D SSQDEAED ...String:
MANEEDDPVV QEIDVYLAKS LAEKLYLFQY PVRPASMTYD DIPHLSAKIK PKQQKVELEM AIDTLNPNYC RSKGEQIALN VDGACADET STYSSKLMDK QTFCSSQTTS NTSRYAAALY RQGELHLTPL HGILQLRPSF SYLDKADAKH REREAANEAG D SSQDEAED DVKQITVRFS RPESEQARQR RVQSYEFLQK KHAEEPWVHL HYYGLRDSRS EHERQYLLCP GSSGVENTEL VK SPSEYLM MLMPPSQEEE KDKPVAPSNV LSMAQLRTLP LADQIKILMK NVKVMPFANL MSLLGPSIDS VAVLRGIQKV AML VQGNWV VKSDILYPKD SSSPHSGVPA EVLCRGRDFV MWKFTQSRWV VRKEVATVTK LCAEDVKDFL EHMAVVRINK GWEF ILPYD GEFIKKHPDV VQRQHMLWTG IQAKLEKVYN LVKETMPKKP DAQSGPAGLV CGDQRIQVAK TKAQQNHALL ERELQ RRKE QLRVPAVPPG VRIKEEPVSE EGEEDEEQEA EEEPMDTSPS GLHSKLANGL PLGRAAGTDS FNGHPPQGCA STPVAR ELK AFVEATFQRQ FVLTLSELKR LFNLHLASLP PGHTLFSGIS DRMLQDTVLA AGCKQILVPF PPQTAASPDE QKVFALW ES GDMSDQHRQV LLEIFSKNYR VRRNMIQSRL TQECGEDLSK QEVDKVLKDC CVSYGGMWYL KGTVQS

UniProtKB: DNA-directed RNA polymerase III subunit RPC5

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Macromolecule #6: DNA-directed RNA polymerase III subunit RPC6

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC6 / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 35.726914 KDa
SequenceString: MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR AVAINRLLSM GQLDLLRSNT GLLYRIKDSQ NAGKMKGSD NQEKLVYQII EDAGNKGIWS RDIRYKSNLP LTEINKILKN LESKKLIKAV KSVAASKKKV YMLYNLQPDR S VTGGAWYS ...String:
MAEVKVKVQP PDADPVEIEN RIIELCHQFP HGITDQVIQN EMPHIEAQQR AVAINRLLSM GQLDLLRSNT GLLYRIKDSQ NAGKMKGSD NQEKLVYQII EDAGNKGIWS RDIRYKSNLP LTEINKILKN LESKKLIKAV KSVAASKKKV YMLYNLQPDR S VTGGAWYS DQDFESEFVE VLNQQCFKFL QSKAETARES KQNPMIQRNS SFASSHEVWK YICELGISKV ELSMEDIETI LN TLIYDGK VEMTIIAAKE GTVGSVDGHM KLYRAVNPII PPTGLVRAPC GLCPVFDDCH EGGEISPSNC IYMTEWLEF

UniProtKB: DNA-directed RNA polymerase III subunit RPC6

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Macromolecule #7: DNA-directed RNA polymerase III subunit RPC7

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC7 / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.95351 KDa
SequenceString: MAGNKGRGRA AYTFNIEAVG FSKGEKLPDV VLKPPPLFPD TDYKPVPLKT GEGEEYMLAL KQELRETMKR MPYFIETPEE RQDIERYSK RYMKVYKEEW IPDWRRLPRE MMPRNKCKKA GPKPKKAKDA GKGTPLTNTE DVLKKMEELE KRGDGEKSDE E NEEKEGSK ...String:
MAGNKGRGRA AYTFNIEAVG FSKGEKLPDV VLKPPPLFPD TDYKPVPLKT GEGEEYMLAL KQELRETMKR MPYFIETPEE RQDIERYSK RYMKVYKEEW IPDWRRLPRE MMPRNKCKKA GPKPKKAKDA GKGTPLTNTE DVLKKMEELE KRGDGEKSDE E NEEKEGSK EKSKEGDDDD DDDAAEQEEY DEEEQEEEND YINSYFEDGD DFGADSDDNM DEATY

UniProtKB: DNA-directed RNA polymerase III subunit RPC7

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Macromolecule #8: DNA-directed RNA polymerase III subunit RPC8

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC8 / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.938846 KDa
SequenceString: MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD ITKLEDAYVF PGDGASHTKV HFRCVVFHPF LDEILIGKI KGCSPEGVHV SLGFFDDILI PPESLQQPAK FDEAEQVWVW EYETEEGAHD LYMDTGEEIR FRVVDESFVD T SPTGPSSA ...String:
MFVLVEMVDT VRIPPWQFER KLNDSIAEEL NKKLANKVVY NVGLCICLFD ITKLEDAYVF PGDGASHTKV HFRCVVFHPF LDEILIGKI KGCSPEGVHV SLGFFDDILI PPESLQQPAK FDEAEQVWVW EYETEEGAHD LYMDTGEEIR FRVVDESFVD T SPTGPSSA DATTSSEELP KKEAPYTLVG SISEPGLGLL SWWTSN

UniProtKB: DNA-directed RNA polymerase III subunit RPC8

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Macromolecule #9: DNA-directed RNA polymerase III subunit RPC9

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC9 / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 16.89399 KDa
SequenceString:
MEVKDANSAL LSNYEVFQLL TDLKEQRKES GKNKHSSGQQ NLNTITYETL KYISKTPCRH QSPEIVREFL TALKSHKLTK AEKLQLLNH RPVTAVEIQL MVEESEERLT EEQIEALLHT VTSILPAEPE AEQKKNTNSN VAMDEEDPA

UniProtKB: DNA-directed RNA polymerase III subunit RPC9

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Macromolecule #10: DNA-directed RNA polymerase III subunit RPC10

MacromoleculeName: DNA-directed RNA polymerase III subunit RPC10 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.354104 KDa
SequenceString:
MLLFCPGCGN GLIVEEGQRC HRFSCNTCPY VHNITRKVTN RKYPKLKEVD DVLGGAAAWE NVDSTAESCP KCEHPRAYFM QLQTRSADE PMTTFYKCCN AQCGHRWRD

UniProtKB: DNA-directed RNA polymerase III subunit RPC10

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Macromolecule #11: DNA-directed RNA polymerases I and III subunit RPAC1

MacromoleculeName: DNA-directed RNA polymerases I and III subunit RPAC1 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 39.301672 KDa
SequenceString: MAASQAVEEM RSRVVLGEFG VRNVHTTDFP GNYSGYDDAW DQDRFEKNFR VDVVHMDENS LEFDMVGIDA AIANAFRRIL LAEVPTMAV EKVLVYNNTS IVQDEILAHR LGLIPIHADP RLFEYRNQGD EEGTEIDTLQ FRLQVRCTRN PHAAKDSSDP N ELYVNHKV ...String:
MAASQAVEEM RSRVVLGEFG VRNVHTTDFP GNYSGYDDAW DQDRFEKNFR VDVVHMDENS LEFDMVGIDA AIANAFRRIL LAEVPTMAV EKVLVYNNTS IVQDEILAHR LGLIPIHADP RLFEYRNQGD EEGTEIDTLQ FRLQVRCTRN PHAAKDSSDP N ELYVNHKV YTRHMTWIPL GNQADLFPEG TIRPVHDDIL IAQLRPGQEI DLLMHCVKGI GKDHAKFSPV ATASYRLLPD IT LLEPVEG EAAEELSRCF SPGVIEVQEV QGKKVARVAN PRLDTFSREI FRNEKLKKVV RLARVRDHYI FSVESTGVLP PDV LVSEAI KVLMGKCRRF LDELDAVQMD

UniProtKB: DNA-directed RNA polymerases I and III subunit RPAC1

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Macromolecule #12: DNA-directed RNA polymerases I and III subunit RPAC2

MacromoleculeName: DNA-directed RNA polymerases I and III subunit RPAC2 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.259222 KDa
SequenceString:
MEEDQELERK ISGLKTSMAE GERKTALEMV QAAGTDRHCV TFVLHEEDHT LGNSLRYMIM KNPEVEFCGY TTTHPSESKI NLRIQTRGT LPAVEPFQRG LNELMNVCQH VLDKFEASIK DYKDQKASRN ESTF

UniProtKB: DNA-directed RNA polymerases I and III subunit RPAC2

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Macromolecule #13: DNA-directed RNA polymerases I, II, and III subunit RPABC1

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC1
type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.584223 KDa
SequenceString: MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN ...String:
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKV YCQRMQEENI TRALIVVQQG MTPSAKQSLV DMAPKYILEQ FLQQELLINI TEHELVPEHV VMTKEEVTEL L ARYKLREN QLPRIQAGDP VARYFGIKRG QVVKIIRPSE TAGRYITYRL VQ

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC1

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Macromolecule #14: DNA-directed RNA polymerases I, II, and III subunit RPABC2

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC2
type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.491026 KDa
SequenceString:
MSDNEDNFDG DDFDDVEEDE GLDDLENAEE EGQENVEILP SGERPQANQK RITTPYMTKY ERARVLGTRA LQIAMCAPVM VELEGETDP LLIAMKELKA RKIPIIIRRY LPDGSYEDWG VDELIITD

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC2

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Macromolecule #15: DNA-directed RNA polymerases I, II, and III subunit RPABC3

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC3
type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 17.162273 KDa
SequenceString:
MAGILFEDIF DVKDIDPEGK KFDRVSRLHC ESESFKMDLI LDVNIQIYPV DLGDKFRLVI ASTLYEDGTL DDGEYNPTDD RPSRADQFE YVMYGKVYRI EGDETSTEAA TRLSAYVSYG GLLMRLQGDA NNLHGFEVDS RVYLLMKKLA F

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC3

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Macromolecule #16: DNA-directed RNA polymerases I, II, and III subunit RPABC4

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC4
type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 7.018244 KDa
SequenceString:
MDTQKDVQPP KQQPMIYICG ECHTENEIKS RDPIRCRECG YRIMYKKRTK RLVVFDAR

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC4

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Macromolecule #17: DNA-directed RNA polymerases I, II, and III subunit RPABC5

MacromoleculeName: DNA-directed RNA polymerases I, II, and III subunit RPABC5
type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 7.655123 KDa
SequenceString:
MIIPVRCFTC GKIVGNKWEA YLGLLQAEYT EGDALDALGL KRYCCRRMLL AHVDLIEKLL NYAPLEK

UniProtKB: DNA-directed RNA polymerases I, II, and III subunit RPABC5

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Macromolecule #18: TATA-box-binding protein

MacromoleculeName: TATA-box-binding protein / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.61573 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAHHHHHHVG TLEVLFQGPS GIVPQLQNIV STVNLGCKLD LKTIALRARN AEYNPKRFAA VIMRIREPRT TALIFSSGKM VCTGAKSEE QSRLAARKYA RVVQKLGFPA KFLDFKIQNM VGSCDVKFPI RLEGLVLTHQ QFSSYEPELF PGLIYRMIKP R IVLLIFVS ...String:
MAHHHHHHVG TLEVLFQGPS GIVPQLQNIV STVNLGCKLD LKTIALRARN AEYNPKRFAA VIMRIREPRT TALIFSSGKM VCTGAKSEE QSRLAARKYA RVVQKLGFPA KFLDFKIQNM VGSCDVKFPI RLEGLVLTHQ QFSSYEPELF PGLIYRMIKP R IVLLIFVS GKVVLTGAKV RAEIYEAFEN IYPILKGFRK TT

UniProtKB: TATA-box-binding protein

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Macromolecule #19: Transcription factor IIIB 50 kDa subunit

MacromoleculeName: Transcription factor IIIB 50 kDa subunit / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 46.587355 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MPGRGRCPDC GSTELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL REVTYSRSTG ENEQVSRSQQ RGLRRVRDLC RVLQLPPTF EDTAVAYYQQ AYRHSGIRAA RLQKKEVLVG CCVLITCRQH NWPLTMGAIC TLLYADLDVF SSTYMQIVKL L GLDVPSLC ...String:
MPGRGRCPDC GSTELVEDSH YSQSQLVCSD CGCVVTEGVL TTTFSDEGNL REVTYSRSTG ENEQVSRSQQ RGLRRVRDLC RVLQLPPTF EDTAVAYYQQ AYRHSGIRAA RLQKKEVLVG CCVLITCRQH NWPLTMGAIC TLLYADLDVF SSTYMQIVKL L GLDVPSLC LAELVKTYCS SFKLFQASPS VPAKYVEDKE KMLSRTMQLV ELANETWLVT GRHPLPVITA ATFLAWQSLQ PA DRLSCSL ARFCKLANVD LPYPASSRLQ ELLAVLLRMA EQLAWLRVLR LDKRSVVKHI GDLLQHRQSL VRSAFRDGTA EVE TREKEP PGWGQGQGEG EVGNNSLGLP QGKRPASPAL LLPPCMLKSP KRICPVPPVS TVTGDENISD SEIEQYLRTP QEVR DFQRA QAARQAATSV PNPP

UniProtKB: Transcription factor IIIB 50 kDa subunit

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Macromolecule #20: Transcription factor TFIIIB component B'' homolog

MacromoleculeName: Transcription factor TFIIIB component B'' homolog / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 55.103477 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MFRRARLSVK PNVRPGVGAR GSTASNPQRG RESPRPPDPA TDSASKPAEP TDVPTVDFGG AEPQEKAPRS STEKTGGDND VEESSRSSS TVSQRRKRIS STSSLVKSSV SVPSESHPLS TINQEAPQPT ATSTKEKQPC SDRYRIYKAQ KLREMLKEEL R KEKKQWKN ...String:
MFRRARLSVK PNVRPGVGAR GSTASNPQRG RESPRPPDPA TDSASKPAEP TDVPTVDFGG AEPQEKAPRS STEKTGGDND VEESSRSSS TVSQRRKRIS STSSLVKSSV SVPSESHPLS TINQEAPQPT ATSTKEKQPC SDRYRIYKAQ KLREMLKEEL R KEKKQWKN KYAINESQRP PDRSKMTMRD FIYYLPDNNP MTSSLEQEKK TEKPSTPVQT REQEGKSTPN AEDNEMEEET DD GPLLVPR VKVAEDGSII LDEESLTVEV LRTKGPCVVE ENDPIFERGS TTTYSSFRKN YYSKPWSNKE TDMFFLAISM VGT DFSMIG QLFPHRARIE IKNKFKREEK TNGWRIDKAF QEKRPFDFDF FAHLLQKVLA EEEKRKQKSV KNHSLKEKKS TKPR KNVKV KKVACEGVNN DPDESMSSRI SDTERSQKDA QTVEEESLTL SREDAEQVAL EVDLNQKKRR RKKQDGANEL GVNNL LENA

UniProtKB: Transcription factor TFIIIB component B'' homolog

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Macromolecule #21: snRNA-activating protein complex subunit 1

MacromoleculeName: snRNA-activating protein complex subunit 1 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 43.074473 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MGTPPGLQTD CEALLSRFQE TDSVRFEDFT ELWRNMKFGT IFCGRMRNLE KNMFTKEALA LAWRYFLPPY TFQIRVGALY LLYGLYNTQ LCQPKQKIRV ALKDWDEVLK FQQDLVNAQH FDAAYIFRKL RLDRAFHFTA MPKLLSYRMK KKIHRAEVTE E FKDPSDRV ...String:
MGTPPGLQTD CEALLSRFQE TDSVRFEDFT ELWRNMKFGT IFCGRMRNLE KNMFTKEALA LAWRYFLPPY TFQIRVGALY LLYGLYNTQ LCQPKQKIRV ALKDWDEVLK FQQDLVNAQH FDAAYIFRKL RLDRAFHFTA MPKLLSYRMK KKIHRAEVTE E FKDPSDRV MKLITSDVLE EMLNVHDHYQ NMKHVISVDK SKPDKALSLI KDDFFDNIKN IVLEHQQWHK DRKNPSLKSK TN DGEEKME GNSQETERCE RAESLAKIKS KAFSVVIQAS KSRRHRQVKL DSSDSDSASG QGQVKATRKK EKKERLKPAG RKM SLRNKG NVQNIHKEDK PLSLSMPVIT EEEENESLSG TEFTASKKRR KH

UniProtKB: snRNA-activating protein complex subunit 1

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Macromolecule #22: snRNA-activating protein complex subunit 4

MacromoleculeName: snRNA-activating protein complex subunit 4 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 165.124812 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MASWSHPQFE KGGGSGGGSW SHPQFEKGGG SENLYFQGSA DVDAEREKIT QEIKELERIL DPGSSGSHVE ISESSLESDS EADSLPSED LDPADPPISE EERWGEASND EDDPKDKTLP EDPETCLQLN MVYQEVIQEK LAEANLLLAQ NREQQEELMR D LAGSKGTK ...String:
MASWSHPQFE KGGGSGGGSW SHPQFEKGGG SENLYFQGSA DVDAEREKIT QEIKELERIL DPGSSGSHVE ISESSLESDS EADSLPSED LDPADPPISE EERWGEASND EDDPKDKTLP EDPETCLQLN MVYQEVIQEK LAEANLLLAQ NREQQEELMR D LAGSKGTK VKDGKSLPPS TYMGHFMKPY FKDKVTGVGP PANEDTREKA AQGIKAFEEL LVTKWKNWEK ALLRKSVVSD RL QRLLQPK LLKLEYLHQK QSKVSSELER QALEKQGREA EKEIQDINQL PEEALLGNRL DSHDWEKISN INFEGSRSAE EIR KFWQNS EHPSINKQEW SREEEERLQA IAAAHGHLEW QKIAEELGTS RSAFQCLQKF QQHNKALKRK EWTEEEDRML TQLV QEMRV GSHIPYRRIV YYMEGRDSMQ LIYRWTKSLD PGLKKGYWAP EEDAKLLQAV AKYGEQDWFK IREEVPGRSD AQCRD RYLR RLHFSLKKGR WNLKEEEQLI ELIEKYGVGH WAKIASELPH RSGSQCLSKW KIMMGKKQGL RRRRRRARHS VRWSST SSS GSSSGSSGGS SSSSSSSSEE DEPEQAQAGE GDRALLSPQY MVPDMDLWVP ARQSTSQPWR GGAGAWLGGP AASLSPP KG SSASQGGSKE ASTTAAAPGE ETSPVQVPAR AHGPVPRSAQ ASHSADTRPA GAEKQALEGG RRLLTVPVET VLRVLRAN T AARSCTQKEQ LRQPPLPTSS PGVSSGDSVA RSHVQWLRHR ATQSGQRRWR HALHRRLLNR RLLLAVTPWV GDVVVPCTQ ASQRPAVVQT QADGLREQLQ QARLASTPVF TLFTQLFHID TAGCLEVVRE RKALPPRLPQ AGARDPPVHL LQASSSAQST PGHLFPNVP AQEASKSASH KGSRRLASSR VERTLPQASL LASTGPRPKP KTVSELLQEK RLQEARAREA TRGPVVLPSQ L LVSSSVIL QPPLPHTPHG RPAPGPTVLN VPLSGPGAPA AAKPGTSGSW QEAGTSAKDK RLSTMQALPL APVFSEAEGT AP AASQAPA LGPGQISVSC PESGLGQSQA PAASRKQGLP EAPPFLPAAP SPTPLPVQPL SLTHIGGPHV ATSVPLPVTW VLT AQGLLP VPVPAVVSLP RPAGTPGPAG LLATLLPPLT ETRAAQGPRA PALSSSWQPP ANMNREPEPS CRTDTPAPPT HALS QSPAE ADGSVAFVPG EAQVAREIPE PRTSSHADPP EAEPPWSGRL PAFGGVIPAT EPRGTPGSPS GTQEPRGPLG LEKLP LRQP GPEKGALDLE KPPLPQPGPE KGALDLGLLS QEGEAATQQW LGGQRGVRVP LLGSRLPYQP PALCSLRALS GLLLHK KAL EHKATSLVVG GEAERPAGAL QASLGLVRGQ LQDNPAYLLL RARFLAAFTL PALLATLAPQ GVRTTLSVPS RVGSESE DE DLLSELELAD RDGQPGCTTA TCPIQGAPDS GKCSASSCLD TSNDPDDLDV LRTRHARHTR KRRRLVAHHH HHHHHHH

UniProtKB: snRNA-activating protein complex subunit 4

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Macromolecule #25: snRNA-activating protein complex subunit 3

MacromoleculeName: snRNA-activating protein complex subunit 3 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 46.812605 KDa
Recombinant expressionOrganism: Trichoplusia ni (cabbage looper)
SequenceString: MAEGSRGGPT CSGVGGRQDP VSGSGGCNFP EYELPELNTR AFHVGAFGEL WRGRLRGAGD LSLREPPASA LPGSQAADSD REDAAVARD LDCSLEAAAE LRAVCGLDKL KCLEDGEDPE VIPENTDLVT LGVRKRFLEH REETITIDRA CRQETFVYEM E SHAIGKKP ...String:
MAEGSRGGPT CSGVGGRQDP VSGSGGCNFP EYELPELNTR AFHVGAFGEL WRGRLRGAGD LSLREPPASA LPGSQAADSD REDAAVARD LDCSLEAAAE LRAVCGLDKL KCLEDGEDPE VIPENTDLVT LGVRKRFLEH REETITIDRA CRQETFVYEM E SHAIGKKP ENSADMIEEG ELILSVNILY PVIFHKHKEH KPYQTMLVLG SQKLTQLRDS IRCVSDLQIG GEFSNTPDQA PE HISKDLY KSAFFYFEGT FYNDKRYPEC RDLSRTIIEW SESHDRGYGK FQTARMEDFT FNDLCIKLGF PYLYCHQGDC EHV IVITDI RLVHHDDCLD RTLYPLLIKK HWLWTRKCFV CKMYTARWVT NNDSFAPEDP CFFCDVCFRM LHYDSEGNKL GEFL AYPYV DPGTFN

UniProtKB: snRNA-activating protein complex subunit 3

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Macromolecule #23: U6_2_Template

MacromoleculeName: U6_2_Template / type: dna / ID: 23 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 30.177291 KDa
SequenceString: (DG)(DT)(DG)(DC)(DT)(DG)(DC)(DC)(DG)(DA) (DA)(DG)(DC)(DG)(DA)(DG)(DC)(DA)(DC)(DG) (DA)(DC)(DT)(DC)(DA)(DA)(DG)(DG)(DG) (DT)(DT)(DA)(DC)(DT)(DG)(DT)(DC)(DA)(DC) (DA) (DC)(DC)(DT)(DA)(DT)(DT) ...String:
(DG)(DT)(DG)(DC)(DT)(DG)(DC)(DC)(DG)(DA) (DA)(DG)(DC)(DG)(DA)(DG)(DC)(DA)(DC)(DG) (DA)(DC)(DT)(DC)(DA)(DA)(DG)(DG)(DG) (DT)(DT)(DA)(DC)(DT)(DG)(DT)(DC)(DA)(DC) (DA) (DC)(DC)(DT)(DA)(DT)(DT)(DT)(DT) (DA)(DA)(DG)(DC)(DC)(DC)(DT)(DT)(DC)(DA) (DA)(DT) (DC)(DA)(DA)(DA)(DT)(DC)(DA) (DT)(DC)(DT)(DT)(DT)(DT)(DG)(DG)(DT)(DT) (DA)(DG)(DG) (DA)(DT)(DA)(DA)(DC)(DT) (DT)(DA)(DT)(DG)(DG)(DT)(DC)(DG)(DG)(DT) (DT)(DT)

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Macromolecule #24: U6_2_Non template

MacromoleculeName: U6_2_Non template / type: dna / ID: 24 / Number of copies: 1 / Classification: DNA
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 30.280418 KDa
SequenceString: (DA)(DA)(DA)(DC)(DC)(DG)(DA)(DC)(DC)(DA) (DT)(DA)(DA)(DG)(DT)(DT)(DA)(DT)(DC)(DC) (DT)(DA)(DA)(DC)(DC)(DA)(DA)(DA)(DA) (DG)(DA)(DT)(DG)(DA)(DT)(DT)(DT)(DG)(DA) (DT) (DT)(DG)(DA)(DA)(DG)(DG) ...String:
(DA)(DA)(DA)(DC)(DC)(DG)(DA)(DC)(DC)(DA) (DT)(DA)(DA)(DG)(DT)(DT)(DA)(DT)(DC)(DC) (DT)(DA)(DA)(DC)(DC)(DA)(DA)(DA)(DA) (DG)(DA)(DT)(DG)(DA)(DT)(DT)(DT)(DG)(DA) (DT) (DT)(DG)(DA)(DA)(DG)(DG)(DG)(DC) (DT)(DT)(DA)(DA)(DA)(DA)(DT)(DA)(DG)(DG) (DT)(DG) (DT)(DG)(DA)(DC)(DA)(DG)(DT) (DA)(DA)(DC)(DC)(DC)(DT)(DT)(DG)(DA)(DG) (DT)(DC)(DG) (DT)(DG)(DC)(DT)(DC)(DG) (DC)(DT)(DT)(DC)(DG)(DG)(DC)(DA)(DG)(DC) (DA)(DC)

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Macromolecule #26: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 26 / Number of copies: 9 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #27: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 27 / Number of copies: 1 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #28: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 28 / Number of copies: 1 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
Component:
ConcentrationName
25.0 mMHEPES
150.0 mMSodium Chloride
5.0 mMMagnesium Chloride
3.0 mMDTT
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 300 / Support film - Material: CARBON / Support film - topology: CONTINUOUS / Support film - Film thickness: 150 / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec.
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Specialist opticsEnergy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV
DetailsFEI Titan Krios G4 (operating at 300 KeV)
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Number real images: 19060 / Average exposure time: 6.26 sec. / Average electron dose: 50.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm / Nominal magnification: 130000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: NONE
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 4.14 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 4.0) / Number images used: 13256
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software: (Name: cryoSPARC (ver. 4.3.1), RELION (ver. 4.0))
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software: (Name: cryoSPARC (ver. 4.3.1), RELION (ver. 4.0))
Final 3D classificationNumber classes: 3 / Software - Name: cryoSPARC (ver. 4.3.1)
FSC plot (resolution estimation)

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