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-Structure paper
| タイトル | Structure-based validation of recombinant light-harvesting complex II. |
|---|---|
| ジャーナル・号・ページ | PNAS Nexus, Vol. 3, Issue 9, Page pgae405, Year 2024 |
| 掲載日 | 2024年9月25日 |
著者 | Soichiro Seki / Tomoko Miyata / Naoko Norioka / Hideaki Tanaka / Genji Kurisu / Keiichi Namba / Ritsuko Fujii / ![]() |
| PubMed 要旨 | Light-harvesting complex II (LHCII) captures sunlight and dissipates excess energy to drive photosynthesis. To elucidate this mechanism, the individual optical properties of pigments in the LHCII ...Light-harvesting complex II (LHCII) captures sunlight and dissipates excess energy to drive photosynthesis. To elucidate this mechanism, the individual optical properties of pigments in the LHCII protein must be identified. In vitro reconstitution with apoproteins synthesized by and pigment-lipid mixtures from natural sources is an effective approach; however, the local environment surrounding each pigment within reconstituted LHCII (rLHCII) has only been indirectly estimated using spectroscopic and biochemical methods. Here, we used cryo-electron microscopy to determine the 3D structure of the rLHCII trimer and found that rLHCII exhibited a structure that was virtually identical to that of native LHCII, with a few exceptions: some C-terminal amino acids were not visible, likely due to aggregation of the His-tags; a carotenoid at the V1 site was not visible; and at site 614 showed mixed occupancy by both chlorophyll and molecules. Our observations confirmed the applicability of the in vitro reconstitution technique. |
リンク | PNAS Nexus / PubMed:39346626 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.17 - 2.57 Å |
| 構造データ | EMDB-38825, PDB-8y15: EMDB-39192, PDB-8yee: ![]() EMDB-39304: Cryo-EM structure of light-harvesting complex II with C1 symmetry ![]() EMDB-39305: Cryo-EM structure of in vitro reconstituted LHCII with C1 symmetry |
| 化合物 | ![]() ChemComp-CHL: ![]() ChemComp-CLA: ![]()
PDB-1lxp: SOLUTION STRUCTURE OF HCV IRES DOMAIN II ![]() ChemComp-NEX: ![]() ChemComp-LHG: ![]() ChemComp-XAT: ![]() ChemComp-HOH: |
| 由来 |
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キーワード | PHOTOSYNTHESIS / Complex / light-harvesting / membrane protein |
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