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TitleStructure of the γ-tubulin ring complex-capped microtubule.
Journal, issue, pagesNat Struct Mol Biol, Year 2024
Publish dateApr 12, 2024
AuthorsAmol Aher / Linas Urnavicius / Allen Xue / Kasahun Neselu / Tarun M Kapoor /
PubMed AbstractMicrotubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules ...Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules assemble with the canonical 13-protofilament architecture, resulting in micrometer-scale α/β-tubulin tracks for intracellular transport that align with, rather than spiral along, the long axis of the filament. We report that the human ~2.3 MDa γ-tubulin ring complex (γ-TuRC), an essential regulator of microtubule formation that contains 14 γ-tubulins, selectively nucleates 13-protofilament microtubules. Cryogenic electron microscopy reconstructions of γ-TuRC-capped microtubule minus ends reveal the extensive intra-domain and inter-domain motions of γ-TuRC subunits that accommodate luminal bridge components and establish lateral and longitudinal interactions between γ-tubulins and α-tubulins. Our structures suggest that γ-TuRC, an inefficient nucleation template owing to its splayed conformation, can transform into a compacted cap at the microtubule minus end and set the lattice architecture of cellular microtubules.
External linksNat Struct Mol Biol / PubMed:38609661
MethodsEM (single particle)
Resolution2.66 - 8.5 Å
Structure data

43482

8vrj

EMDB-43482: Density map for gamma tubulin ring complex capped microtubule
PDB-8vrj: Rigid body fitted model for gamma tubulin ring complex capped microtubule
Method: EM (single particle) / Resolution: 7.7 Å

43483

8vrk

EMDB-43483: Refined density map of gamma tubulin ring complex capped microtubule
PDB-8vrk: Rigid body fitted model for refined density map of gamma tubulin ring complex capped microtubule
Method: EM (single particle) / Resolution: 8.5 Å

43519

8vt7

EMDB-43519, PDB-8vt7:
Structure of the gamma tubulin ring complex nucleated microtubule protofilament.
Method: EM (single particle) / Resolution: 2.66 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM / Guanosine diphosphate

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
KeywordsCELL CYCLE / Microtubule nucleation complex bound to a microtubule / Microtubule

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