EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of the γ-tubulin ring complex-capped microtubule.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 31, Issue 7, Page 1124-1133, Year 2024
掲載日	2024年4月12日
著者	Amol Aher / Linas Urnavicius / Allen Xue / Kasahun Neselu / Tarun M Kapoor /
PubMed 要旨	Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules ...Microtubules are composed of α-tubulin and β-tubulin dimers positioned head-to-tail to form protofilaments that associate laterally in varying numbers. It is not known how cellular microtubules assemble with the canonical 13-protofilament architecture, resulting in micrometer-scale α/β-tubulin tracks for intracellular transport that align with, rather than spiral along, the long axis of the filament. We report that the human ~2.3 MDa γ-tubulin ring complex (γ-TuRC), an essential regulator of microtubule formation that contains 14 γ-tubulins, selectively nucleates 13-protofilament microtubules. Cryogenic electron microscopy reconstructions of γ-TuRC-capped microtubule minus ends reveal the extensive intra-domain and inter-domain motions of γ-TuRC subunits that accommodate luminal bridge components and establish lateral and longitudinal interactions between γ-tubulins and α-tubulins. Our structures suggest that γ-TuRC, an inefficient nucleation template owing to its splayed conformation, can transform into a compacted cap at the microtubule minus end and set the lattice architecture of cellular microtubules.
リンク	Nat Struct Mol Biol / PubMed:38609661 / PubMed Central
手法	EM (単粒子)
解像度	2.66 - 8.5 Å
構造データ	43085 8va2 EMDB-43085, PDB-8va2: Symmetry expanded map of 2 gamma-tubulins bound to 2 alpha tubulins in gamma tubulin ring complex capped microtubule end. 手法: EM (単粒子) / 解像度: 4.5 Å 43481 8vrd EMDB-43481: Density map for free recombinant gamma tubulin ring complex PDB-8vrd: Rigid body fitted model for free recombinant gamma tubulin ring complex. 手法: EM (単粒子) / 解像度: 7.0 Å 43482 8vrj EMDB-43482: Density map for gamma tubulin ring complex capped microtubule PDB-8vrj: Rigid body fitted model for gamma tubulin ring complex capped microtubule 手法: EM (単粒子) / 解像度: 7.7 Å 43483 8vrk EMDB-43483: Refined density map of gamma tubulin ring complex capped microtubule PDB-8vrk: Rigid body fitted model for refined density map of gamma tubulin ring complex capped microtubule 手法: EM (単粒子) / 解像度: 8.5 Å 43519 8vt7 EMDB-43519, PDB-8vt7: Structure of the gamma tubulin ring complex nucleated microtubule protofilament. 手法: EM (単粒子) / 解像度: 2.66 Å
化合物	ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM
由来	Trichoplusia ni (イラクサキンウワバ) homo sapiens (ヒト)
キーワード	CELL CYCLE / gamma tubulin ring complex / CYTOSOLIC PROTEIN / Complex / Microtubule nucleation complex bound to a microtubule / Microtubule