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TitleEbola and Marburg virus matrix layers are locally ordered assemblies of VP40 dimers.
Journal, issue, pagesElife, Vol. 9, Year 2020
Publish dateOct 5, 2020
AuthorsWilliam Wan / Mairi Clarke / Michael J Norris / Larissa Kolesnikova / Alexander Koehler / Zachary A Bornholdt / Stephan Becker / Erica Ollmann Saphire / John Ag Briggs /
PubMed AbstractFiloviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of ...Filoviruses such as Ebola and Marburg virus bud from the host membrane as enveloped virions. This process is achieved by the matrix protein VP40. When expressed alone, VP40 induces budding of filamentous virus-like particles, suggesting that localization to the plasma membrane, oligomerization into a matrix layer, and generation of membrane curvature are intrinsic properties of VP40. There has been no direct information on the structure of VP40 matrix layers within viruses or virus-like particles. We present structures of Ebola and Marburg VP40 matrix layers in intact virus-like particles, and within intact Marburg viruses. VP40 dimers assemble extended chains via C-terminal domain interactions. These chains stack to form 2D matrix lattices below the membrane surface. These lattices form a patchwork assembly across the membrane and suggesting that assembly may begin at multiple points. Our observations define the structure and arrangement of the matrix protein layer that mediates formation of filovirus particles.
External linksElife / PubMed:33016878 / PubMed Central
MethodsEM (subtomogram averaging) / X-ray diffraction
Resolution2.46 - 12.8 Å
Structure data

EMDB-11660:
Structure of VP40 matrix layer in EBOV NP-VP24-VP35-VP40 VLPs
Method: EM (subtomogram averaging) / Resolution: 10.2 Å

EMDB-11661:
Structure of VP40 matrix layer in EBOV VP40 VLPs
Method: EM (subtomogram averaging) / Resolution: 9.8 Å

EMDB-11662:
Structure of VP40 matrix layer in EBOV VP40-GP VLPs
Method: EM (subtomogram averaging) / Resolution: 9.9 Å

EMDB-11663:
Structure of VP40 matrix layer in intact MARV virions
Method: EM (subtomogram averaging) / Resolution: 9.6 Å

EMDB-11664:
Structure of VP40 matrix layer in MARV VP40 VLPs
Method: EM (subtomogram averaging) / Resolution: 10.8 Å

EMDB-11665:
Structure of GP in EBOV VP40-GP VLPs
Method: EM (subtomogram averaging) / Resolution: 12.8 Å

PDB-7jzj:
Crystal structure demonstrating CTD-CTD interactions of Zaire Ebola virus VP40 dimer
Method: X-RAY DIFFRACTION / Resolution: 2.46 Å

PDB-7jzt:
Low resolution crystal structure of Zaire Ebola virus VP40 in space group P6422
Method: X-RAY DIFFRACTION / Resolution: 3.77 Å

Chemicals

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM / Polyethylene glycol

ChemComp-HOH:
WATER / Water

Source
  • Ebola virus - Mayinga
  • Marburg virus - Musoke
  • zaire ebolavirus (strain mayinga-76)
KeywordsVIRAL PROTEIN / VP40 / matrix protein / Ebola virus

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