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Structure paper

TitleStructure of the 70S Ribosome from the Human Pathogen Acinetobacter baumannii in Complex with Clinically Relevant Antibiotics.
Journal, issue, pagesStructure, Vol. 28, Issue 10, Page 1087-11100.e3, Year 2020
Publish dateOct 6, 2020
AuthorsDavid Nicholson / Thomas A Edwards / Alex J O'Neill / Neil A Ranson /
PubMed AbstractAcinetobacter baumannii is a Gram-negative bacterium primarily associated with hospital-acquired, often multidrug-resistant (MDR) infections. The ribosome-targeting antibiotics amikacin and ...Acinetobacter baumannii is a Gram-negative bacterium primarily associated with hospital-acquired, often multidrug-resistant (MDR) infections. The ribosome-targeting antibiotics amikacin and tigecycline are among the limited arsenal of drugs available for treatment of such infections. We present high-resolution structures of the 70S ribosome from A. baumannii in complex with these antibiotics, as determined by cryoelectron microscopy. Comparison with the ribosomes of other bacteria reveals several unique structural features at functionally important sites, including around the exit of the polypeptide tunnel and the periphery of the subunit interface. The structures also reveal the mode and site of interaction of these drugs with the ribosome. This work paves the way for the design of new inhibitors of translation to address infections caused by MDR A. baumannii.
External linksStructure / PubMed:32857965 / PubMed Central
MethodsEM (single particle)
Resolution2.5 - 3.0 Å
Structure data

10809

6yhs

EMDB-10809, PDB-6yhs:
Acinetobacter baumannii ribosome-amikacin complex - 50S subunit
Method: EM (single particle) / Resolution: 2.7 Å

10869

6ypu

EMDB-10869, PDB-6ypu:
Acinetobacter baumannii ribosome-amikacin complex - 30S subunit body
Method: EM (single particle) / Resolution: 2.9 Å

10892

6ys5

EMDB-10892, PDB-6ys5:
Acinetobacter baumannii ribosome-amikacin complex - 30S subunit head
Method: EM (single particle) / Resolution: 3.0 Å

10898

6ysi

EMDB-10898, PDB-6ysi:
Acinetobacter baumannii ribosome-tigecycline complex - 50S subunit
Method: EM (single particle) / Resolution: 2.5 Å

10914

6yt9

EMDB-10914, PDB-6yt9:
Acinetobacter baumannii ribosome-tigecycline complex - 30S subunit body
Method: EM (single particle) / Resolution: 2.7 Å

10915

6ytf

EMDB-10915, PDB-6ytf:
Acinetobacter baumannii ribosome-tigecycline complex - 30S subunit head
Method: EM (single particle) / Resolution: 3.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

ChemComp-AKN:
(2S)-N-[(1R,2S,3S,4R,5S)-4-[(2R,3R,4S,5S,6R)-6-(aminomethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-5-azanyl-2-[(2S,3R,4S,5S / medication, antibiotic*YM

ChemComp-T1C:
TIGECYCLINE / medication, antibiotic*YM

Source
  • acinetobacter baumannii atcc 19606 = cip 70.34 = jcm 6841 (bacteria)
  • acinetobacter baumannii (strain atcc 19606 / dsm 30007 / cip 70.34 / jcm 6841 / nbrc 109757 / ncimb 12457 / nctc 12156 / 81) (bacteria)
  • acinetobacter baumannii (bacteria)
KeywordsRIBOSOME / antibiotic / amikacin / translation / tigecycline

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